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Antimicrobial,hemagglutination and phytotoxic activity of crude ethanolic and aqueous extracts of Seriphidium kurramense

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Journal of Traditional Chinese Medicine 2018年第3期38卷 433-438页

作者： Kafeel Ahmad Amjad Ali Waqar Ahmed Afridi Ramla Somayya Muhammad Jawad Ullah centre of biotechnology and microbiology University of Peshawar

OBJECTIVE: To investigate the antimicrobial activity, hemagglutination and phytotoxic activity of crude ethanolic and aqueous extracts of Seriphidium ***: The extracts were analyzed by agar well diffusion assays against five bacterial species:Staphylococcus aureus(S. aureus), methicillin-resistant S. aureus, Escherichia coli, Klebsiella pneumoniae, Bacillus subtilis, and Salmonella typhi. The extracts were also screened against six fungal species — Aspergillus niger, Aspergillus flavus, Alternaria solani, Rhizoctonia solani, Fusarium solani and Pleurotus florida — using the agar tube diffusion method. Additionally, hemagglutination and phytotoxic activities of the crude ethanolic and aqueous extracts were ***: The crude ethanolic and aqueous extracts showed dose-dependent inhibition of the various tested fungal and bacterial strains. No hemagglutination activity was observed. Both the ethanolic and aqueous extracts showed dose-dependent phytotoxic activity toward Lemna ***: The crude ethanolic and aqueous extracts of Seriphidium kurramense possess good antimicrobial and phytotoxic activities, but no hemagglutination activity.

关键词： Anti-bacterial agents Antifungal agents Phytotoxic Seriphidium kurramense

Phytochemical screening, antioxidant and antibacterial properties of daphne mucronata

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Journal of Traditional Chinese Medicine 2019年第6期39卷 764-771页

作者： Ghosia Lutfullah Asma Shah Kafeel Ahmad Jamila Haider centre of biotechnology and microbiology University of Peshawar

OBJECTIVE:To aim at preliminary phytochemical screening of Daphne mucronata and evaluate its antioxidant and antibacterial ***:Preliminary phytochemical screening was conducted for the crude extracts using standard *** properties of crude methanolic extracts,n-hexane,chloroform,ethyl acetate and aqueous fractions of leaves,roots and bark were evaluated following standard *** antibacterial activities were checked against Acinetobacter baumannii(***),Escherichia coli(***),Pseudomonas aeruginosa(***),Staphylococcus aureus(***),Morganella morganii(***)and vancomycin resistant ***(VRSA).RESULTS:Carbohydrates,saponins,steroids,phenols/tannins,flavonoids and glycosides were present in different parts of Daphne *** extracts showed good antioxidant activity with EC50 values of 157.82-361.61μg/m *** extracts of roots showed good activity against ***(86.95%),***(85.18%)and ***(84.61%).Methanolic extracts of bark were active against ***(65.21%),***(65.21%)and ***(62.96%).Methanolic extracts of leaves showed good activity against ***(78.26%),***(77.78%),***(74.07%),***(73.07%),***(69.56%),VRSA(68%)and Proteus vulgaris(60%).The n-hexane fraction of roots was effective against ***(78.26%).Chlorofom fraction of roots showed moderate activity against ***(60.86%)and ***(61.53%).Ethyl acetate fraction of roots showed moderate activity against ***(69.56%),***(62.96%)and ***(69.23%).CONCLUSION:This study illustrates that Daphne mucronata possesses good antioxidant and antibacterial *** plant could be further exploited as potential natural antioxidant and as a new source of antimicrobials for treatment of various infections.

关键词： Antioxidants Anti-bacterial agents Phytochemicals Daphne mucronata

Antifungal, phytotoxic and hemagglutination activity of methanolic extracts of Ocimum basilicum

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Journal of Traditional Chinese Medicine 2016年第6期36卷 794-798页

作者： Kafeel Ahmad Ali talha Khalil Yusra Ramla Somayya centre of biotechnology and microbiology University of Peshawar

OBJECTIVE: To report some of the pharmacological features of the medicinal plant Ocimum ***: In the current studies, Antifungal activity of crude methanolic fraction of Ocimum basilicum was determined against eight pathogenic fungal strains using tube dilution assay. The methanolic fraction was also investigated for phytotoxic and hemagglutination ***: Of the eight strains investigated only Candida albicans and Curvilaria lunata were found to be least affected by plant extract while the rest were significantly inhibited. Moderate phytotoxic activity was observed against lemna minor. Hemagglutination activity showed absence of phytolectins and hence no agglutination of ***: The crude extract of Ocimum basilicum has significant properties against fungi and phytotoxic substances.

关键词： Plants,toxic Antifungal agents Pathogenicity Ocimum basilicum

Professor Arnold L.(“Arny”)Demain (1927-2020):leaving a legacy in the field of Industrial microbiology and biotechnology;a personal appraisal

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Synthetic and Systems biotechnology 2020年第4期5卷 324-327页

作者： Erick J.Vandamme Dept.biotechnology Centre for Industrial Biotechnology and Synthetic BiologyFac.Bioscience EngineeringGhent UniversityGhentBelgium

*** This article is a tribute to-and sadly-an“in memoriam”of Professor Arnold(Arny)***(born 26 April,1927,Brooklyn,New York,USA–deceased on 3 April 2020,Madison,New Jersey,USA).It aims to illustrate his worldwide impact over six decades in the field of industrial microbiology and biotechnology and to testify of his great ability to establish cordial personal human relationships and to create social networks with his students,former collaborators and also with the research and corporate biotech-sector worldwide.

关键词： field illustrate Arnold

Nutritive and Microbiological Quality of Fish Feed Formulated from Local Flours Enriched with Larvaes of Hermetia illucens

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Advances in microbiology 2025年第1期15卷 30-41页

作者： Francine Herodias Béké Nadège Kouadio-N’gbesso Paul Yao Attien N’da Einstein Kouadio Audrey Charlène Asté Comoé Koffi Donatien Benie Adjehi Thomas Dadié Department of Food Sciences and Technologies (STA) Laboratory of Microbiology and Biotechnology Food Nangui Abrogoua University Abidjan Cte dIvoire Laboratory of microbiology Oceanographic Research Center Abidjan Cte dIvoire Laboratory of Agrovalorisation Jean Lorougnon Guede University Daloa Cte dIvoire Agronomic Sciences and Processing Institut National Polytechnique Flix Houphout-Boigny Yamoussoukro Cte dIvoire Department of Biosciences Laboratory of Biotechnology Agriculture and valorization of Biological Resources (LBAVR) Flix Houphout Boigny University Abidjan Cte dIvoire

In aquaculture, feed represents the main component of production costs, and the development of this sector depends on the development of an economical feed formulation that meets the qualitative and quantitative requirements of fish. The aim of this study was to determine the nutritive and microbiological quality of fish feed formulated from local flours enriched with Hermetia illucens larvae. The raw materials used for formulation were fishmeal, corn meal, low-grade rice, soybean meal and Hermetia illucens larvae meal. Different iso-protein feed compositions were prepared with 0%, 10%, 25%, 35%, 50%, 65%, 75% and 100% incorporation of Hermetia illucens larvae meal as a substitute for fish meal. Biochemical and microbiological analyses of these flours were determined using standard methods. The results showed that incorporation of larvae meal had an influence on the biochemical characteristics ash (8.15 to 20.27%), lipid (11.55 to 24.94%), fiber (13.93 to 20.41%) and dry matter (89.65 to 91.19%) of various formulated feed. Loads of fecal Streptococci, Staphylococci, Aeromonas, yeasts and molds ranged from 2.4 to 4.9 log 10 CFU/g;3.6 to 3.9 log 10 CFU/g;2.2 to 2.7 log 10 CFU/g;2.1 to 2.3 log 10 CFU/g, respectively. The level of contamination of these flours was below the microbiological criteria applicable to animal feed. Feed formulated with 0% and 10% Hermetia illucens larvae showed the best nutritive and microbiological characteristics. These results suggest that flours enriched with Hermetia illucens larvae could be used in fish feed.

关键词： Hermetia illucens Fish Feed Nutritive and Microbiological Quality

Characterisation of Resistance Potential to Biofilms Microbial Contaminating Thaumatococcus daniellii and Musa paradisiaca Leaves Used as Food Packaging

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American Journal of Plant Sciences 2025年第1期16卷 180-194页

作者： Comoé Koffi Donatien Benie N’zebo Désiré Kouame Koua Atobla Yao Paul Attien Wako-Tianwa Alice Tuo Adjaratou Traore Noutenin Joelle Diarrassouba Adjehi Dadie Mireille Dosso Department of Biosciences Laboratory of Biotechnology Agriculture and Valorization of Biological Resources University of Flix Houphout Boigny Abidjan Cte dIvoire Department of Food Science and Technology Laboratory of Biotechnology and Food Microbiology (LMBM) University of Nangui-Abrogoua Abidjan Cte dIvoire Department of Bacteriology and Virology Institut Pasteur of Cte dIvoire (IPCI) Abidjan Cte dIvoire Department of Biochemistry-microbiology Laboratory of Agrovalorisation University of Jean Lorougnon GUEDE Daloa Cte dIvoire Department of Medical Sciences University of Alassane Ouattara University Hospital Center (UHC) of Bouak Bouak Cte dIvoire

Despite their biodegradability and economic advantage, plant leaves used as packaging can constitute a public health problem. The aim of this study was to characterize the microbial diversity contaminating plant leaves used as food packaging. In total, two hundred and forty (240) samples composed of Thaumatococcus daniellii and Musa paradisiaca leaves were collected and analyzed. Microbial diversity was assessed using specific medium and biochemical tests. The resistance profile was determined by the Müeller-Hinton agar diffusion method. The resistance (blaSHV, blaIMP, blaTEM) and biofilm formation (pslA, pelA) genes were searched by PCR method. Plant leaves were contaminated by bacterial (68.7%) and fungal (100%) strains. Extreme bacterial (7.1 log10 cfu/cm2) and fungal (3.5 log10 cfu/cm2) loads were obtained on Thaumatococcus daniellii leaves. Bacterial prevalence was 45.1% (S. aureus), 38.8% (E. coli) and 16.1 (P. aeruginosa). In order of decreasing importance, the prevalence of fungal species was 41.1% (A. flavus), 33.1% (A. fumigattus), 13.7% (A. niger) and 12.1% Candida sp. Resistance of E. coli to penicillins ranges from 31.6% to 87.3% and to cephalosporins from 13.3% to 28%. The P. aeruginosa strains were mainly resistant to aztreonam (87.6%). Those of S. aureus showed resistance to tetracycline (67.6), vancomycin (53), erythromycin (44.6) and levofloxacin (32.7). The blaSHV (14.28% to 18.60%) and blaIMP (9.52% to 16.28%) genes were detected in the bacterial strains. P. aeruginosa strains (19.05%) harbored the pslA and pelA genes. The health safety of these biodegradable plant-based packaging contributes to their valorization.

关键词： Resistance Biofilm Genes Thaumatococcus daniellii Musa paradisiaca

Gut virome:New key players in the pathogenesis of inflammatory bowel disease

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World Journal of Methodology 2025年第2期15卷 27-43页

作者： Helal F Hetta Rehab Ahmed Yasmin N Ramadan Hayam Fathy Mohammed Khorshid Mohamed M Mabrouk Mai Hashem Department of Medical microbiology and Immunology Faculty of MedicineAssiut UniversityAssiut 71515Egypt Division of microbiology Immunology and BiotechnologyFaculty of pharmacyUniversity of TabukTabuk 71491Saudi Arabia Division of microbiology Immunology and BiotechnologyDepartment of Natural Products and Alternative MedicineFaculty of PharmacyUniversity of TabukTabuk 71491Saudi Arabia Department of Internal Medicine Division HepatogastroenterologyAssiut UniversityAssiut 71515Egypt Department of Clinical Research Egyptian Developers of Gastroenterology and Endoscopy FoundationCairo 11936Egypt Department of Internal Medicine Faculty of Medicine.Tanta UniversityTanta 31527Egypt Department of Tropical Medicine Gastroenterology and HepatologyAssiut University HospitalAssiut 71515Egypt

Inflammatory bowel disease(IBD)is a chronic inflammatory illness of the *** the mechanism underlying the pathogenesis of IBD is not fully under-stood,it is believed that a complex combination of host immunological response,environmental exposure,particularly the gut microbiota,and genetic suscept-ibility represents the major *** gut virome is a group of viruses found in great frequency in the gastrointestinal tract of *** gut virome varies greatly among individuals and is influenced by factors including lifestyle,diet,health and disease conditions,geography,and *** majority of research has focused on the significance of gut bacteria in the progression of IBD,although viral populations represent an important component of the *** conducted this review to highlight the viral communities in the gut and their expected roles in the etiopathogenesis of IBD regarding published research to date.

关键词： Inflammatory bowel disease Pathogenesis Gut virome Bacteriophage Eukaryotic viruses

Isolation of a salt tolerant laccase secreting strain of Trichoderma sp. NFCCI-2745 and optimization of culture conditions and assessing its efectiveness in treating saline phenolic efuents

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Journal of Environmental Sciences 2013年第12期25卷 2410-2416页

作者： L.M.Divya G.K.Prasanth C.Sadasivan Department of biotechnology and microbiology Kannur University

Most of the hazardous pollutants are phenolic in nature and persists in the environment. The ability of laccases to oxidize phenolic compounds and reduce molecular oxygen to water has led to intensive studies of these enzymes. Therefore the fungal strains with high laccase activity and substrate affinity that can tolerate harsh environmental conditions have a potential for biotechnological applications. Salt tolerant laccase secreting fungi can be utilized in treatment of saline and phenolic rich industrial effluents such as coir effluent and textile effluent that needed to be diluted several fold before microbial treatment. This is the first study describing the isolation and optimization of a salt tolerant strain of Trichoderma sp. potential for industrial applications. The fungus was identified based on morphological characteristics and was subsequently confirmed with molecular techniques and deposited at National Fungal Culture Collections of India （NFCCI） under the Accession No. Trichoderma viride NFCCI 2745. In contrast to other laccase secreting fungi, light conditions did not exert much influence on laccase production of this strain and salinity enhanced its laccase secretion. The fungus effectively removed the phenolic content of the textile effluent, coir-ret liquor and wood processing effluent within 96 hr of incubation. The tolerance of the fungus to high salinity and phenolic compounds makes this strain ideal for treating saline and phenolic rich industrial effluents.

关键词： laccase optimization phenol tolerant salt tolerant Trichoderma viride

Single carbon metabolism–A new paradigm for microbial bioprocesses?

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Synthetic and Systems biotechnology 2024年第2期9卷 322-329页

作者： Michael Baumschabl Özge Ata Diethard Mattanovich Austrian centre of Industrial biotechnology(ACIB) Vienna1190Austria University of Natural Resources and Life Sciences Department of BiotechnologyInstitute of Microbiology and Microbial BiotechnologyViennaAustria

Increasing atmospheric carbon dioxide levels,a reduction of arable land area and the dependence of first and second generation biotechnology feedstocks on agricultural products,call for alternative,sustainable feedstock sources for industrial *** direct use of CO_(2)or conversion of CO_(2)into other single carbon(C1)sources have great potential as they might help to reduce carbon emissions and do not compete with agricultural land *** we discuss the microbial use of C1 carbon sources,their potential applications in biotechnology,and challenges towards sustainable C1-based industrial biotechnology *** focus on methanol,formic acid,methane,syngas,and CO_(2)as feedstocks for bioprocesses,their assimilation pathways,current and emerging applications,and limitations of their *** mini-review is intended as a first introduction for researchers who are new to the field of C1 biotechnology.

关键词： Sustainability BioeconomyCarbon dioxide Methanol Formate Methane