检索结果-南通市图书馆

Effect of Gubenfangxiao decoction on respiratory syncytial virus-induced asthma and expression of asthma susceptibility gene orosomucoid 1-like protein 3 in mice

引用

Journal of Traditional Chinese Medicine 2016年第1期36卷 101-106页

作者： Huang Zhengguang Gao Lijuan Zhao Xia Ling Hao Chen Wenjun Institute of Pediatrics of Nanjing University of Chinese Medicine Jiangsu Key Laboratory of Pediatric Respiratory Disease

OBJECTIVE： To investigate the effect of Gubenfangxiao decoction （GBFXD） on respiratory-syncytial-virus （RSV） -induced asthma and the expression of asthma susceptibility gene, orosomucoid 1-1ike protein 3 （ORMDL3） in mice. METHODS： Seventy-two female BALB/c mice were randomly assigned to normal, model, GBFXD high dose, GBFXD moderate dose, GBFXD low dose and montelukast groups. An asthma model was induced via intraperitoneal injection and aerosol inhalation of ovalbumin （OVA） and repeated intranasal instillation of RSV in all mice, except those in the normal group. All treatments were administered at the first onset of asthma （within 8 weeks of model establishment） and the mice were euthanized after 28 days of treatment. The levels of transforming growth factor-β （TGF-β） and interleukin-6 （IL-6） in bronchoalveolar lavage fluid （BALF） of the mice were measured and the expression of asthma sus- ceptibility gene ORMDL3 in lung tissue was deter- mined using real-time polymerase chain reaction （RT-PCR） and western blotting. RESULTS： Expression of ORMDL3 and levels of TGF-β and IL-6 were significantly higher in the mod- el group （P 〈 0.05, P 〈 0.01） compared with the normal mice. Levels of ORMDL3, TGF-β and IL-6 were significantly lower in all three GBFXD treated groups （P 〈 0.05） compared with the model group. However, the levels in the GBFXD treatment groups did not differ significantly from the montelukast group. CONCLUSION： GBFXD had a therapeutic effect in this experimental model. The functional mechanism of GBFXD may involve multiple factors, including alleviation of airway inflammation, down-regulation of asthma susceptibility gene ORMDL3 and inhibition of airway remodeling.

关键词： asthma ORMDL3 protein, mouse Air-way remodeling Gubenfangxiao decoction

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Interleukin-12 was not involved in promotion of T helper cell differentiation induced by theophylline

引用

Acta Pharmacologica Sinica 2004年第12期25卷 100-104页

作者： Jiong YANG1, Wei GUO, Han-xiang NIE, Su-ping HU Departmentof respiratory disease, Renmin Hospital, Wuhan University, Wuhan 430060, China Department of respiratory disease Renmin Hospital Wuhan University Wuhan 430060

AIM: To investigate the effect of theophylline on the naive T cell differentiation and the probable role of interleukin- 12 (IL-12). METHODS: Naive cord blood T cells were treated with theophylline 10 mg/L for 3 d after stimulation with PHA 100 mg/L. Differentiation of Tcells was analyzed by flow cytometry. Theophylline 10 mg/L and IL-12- mAb 0.025 mg/L were added in cord blood mononuclear cell (CBMC) cultures primed with LPS 1 mg/L to detect the levels of IL-12 and IL-12P40. The whole blood cultures were obtained from twelve health volunteers with or without administration of theophylline (200 mg). Cytokines were measured by enzyme linked immuno-sorbent assay. RESULTS: Theophylline promoted T helper 1 (Th1) cells differentiation from naive T cells (21.9 %±10.3 % vs 9.4 %±5.6 %, P<0.05), but had no significant effect on Th2 deviation. But theophylline inhibited the production of IL-12 and IL-12P40 by CBMC in vitro (28±6 ng/L vs 57±14 ng/L and 88±34 ng/L vs 214±82 ng/L, P<0.01) and reduced IL-12 and IL-12P40 levels in whole blood cultures from healthy subjects (19±11 ng/L vs 31±15 ng/L and 92±13 ng/L vs 196±49 ng/L, P<0.01). CONCLUSION: Theophylline promoted the differentiation of Th1 cells. IL- 12 seemed not to be involved in this process.

关键词： asthma theophylline helper inducer T lymphocytes Th1 cells Th2 cells interleukin-12

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

The Effect of Ginkgo Biloba Extract on the Expression of PKCα in the Inflammatory Cells and the Level of IL-5 in Induced Sputum of asthmatic Patients

引用

Journal of Huazhong University of Science and Technology(Medical Sciences) 2007年第4期27卷 375-380页

作者：唐以军徐永健熊盛道倪望陈仕新高宝安叶涛曹勇杜春玲 The Department of respiratory disease Taihe Hospital of Yunyang Medical College Department of respiratory disease Tongji HospitalTongji Medical CollegeHuazhong University of Science and Technology

To investigate the effect of the Ginkgo Biloba Extract （GBE） on the asthma and examine its possible mechanisms, 75 asthma patients were divided into 4 groups and the patients were respectively treated with fluticasone propionate for 2 weeks or 4 weeks, or treated with fluticasone propionate plus GBE for 2 weeks or 4 weeks. Fifteen healthy volunteers served as healthy controls. Sputum inhalation with inhaling hypertonic saline （4%-5%） was performed. Lung ventilatory function and forced expiratory volume in one second （FEVI） were measured. The numbers of different cells in induced sputum were calculated. The expression of PKCα in the cells was immunocytochemically detected and the percentages of positive cells in different cells were counted. Interleukin-5 （IL-5） in sputum supernatants was detected with enzyme-linked immunosorbent assay. The percentage of eosinophils, lymphocytes, PKCα positive inflammatory cells and the concentration of IL-5 in asthmatic patients were higher than those in the controls （P〈0.05）, and the eosinophils, lymphocytes, positive expression of PKCα and the level of IL-5 were significantly decreased in asthmatic patients after they were treated with fluticasone propionate or fluticasone propionate plus GBE. However, they were still significantly higher than those of the controls. Compared to the group treated with glucocorticosteroid for 2 weeks, no significant decrease was found in the percentage of eosinophils, lymphocytes, PKCα positive inflammatory cells and the IL-5 in the supernatant of induced sputum. Compared with the group treated with glucocorticosteroid for 2 or 4 weeks, significant decrease in the same parameters was observed in the group treated with fluticasone propionate and GBE for 4 weeks. The IL-5 level in the supernatant of induced sputum was positively correlated with the percentage of PKCα-positive inflammatory cells and the percentage of eosinophils in the induced sputum in asthma patient groups respectively （n=150, r=0.83

关键词： bronchial asthma glucocorticosteroid Ginkgo Biloba Extract proteinase C interleukin-5

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Workup of difficult-to-treat asthma:implications from treatable

引用

Precision Clinical Medicine 2023年第1期6卷 30-37页

作者： Qing Zhang Wen Wen Wu Lei Li Vanessa M.McDonald Yu Cheng Chen Gang Wang Peter G.Gibson Department of respiratory and Critical Care Medicine Clinical Research Center for Respiratory DiseaseWest China HospitalSichuan UniversityChengdu 610041SichuanChina Laboratory of Pulmonary Immunology and Inflammation Frontiers Science Center for Disease-related Molecular NetworkSichuan UniversityChengdu 610041SichuanChina Center of Excellence in Severe asthma and Priority Research Center for asthma and respiratory disease the University of NewcastleNewcastle 2308Australia National Health and Medical Research Council Center for Research Excellence in Severe asthma and Treatable Traits the University of NewcastleNewcastle 2308Australia Cardiology Division Department of MedicineWest China HospitalSichuan UniversityChengdu 610041SichuanChina Department of respiratory and Sleep Medicine John Hunter HospitalHunter Medical Research InstituteNewcastle 2305Australia

Traditional stepwise approach usually adjusts the treatment regimen based on changes in asthma symptoms and severity to achieve good asthma ***,due to the generalized heterogeneity and complexity of asthma,its therapeutic efficacy in difficultto-treat asthma is ***,a precision medicine approach based on the identification and intervention of treatable traits of chronic airway disease has been proposed and appears to be of greater benefit to *** reported a 71-year-old male with uncontrolled asthma and multiple exacerbations over the past *** complained of persistent dyspnea despite high-dose of inhaled corticosteroids plus other *** this patient have some potential treatable traits contributing to difficult-to-treat asthma?Through a multidimensional assessment of three domains including pulmonary,extrapulmonary,and behavioral/risk factors,15 treatable traits were identified in the patient,mainly including airflow limitation,eosinophilic airway inflammation,small airway dysfunction,exacerbation prone,dilated cardiomyopathy,diabetes mellitus,inhaler device polypharmacy,smoking,and the absence of an asthma action *** targeted treatment for these treatable traits,the patient experienced significant improvement in dyspnea and he could maintain good asthma control with low-dose inhaled corticosteroids and long-acting β_(2)-*** study shows that,in response to the limitation of a stepwise approach to therapy,treatable traits is a new strategy where patients are individually assessed for a specified set of treatable problems,and an individualized treatment program is developed and implemented based on this multidimensional assessment,especially for difficult-to-treat asthma.

关键词： difficult-to-treat asthma treatable traits multidimensional assessment comorbidity stepwise approach

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Impaired CTLA-4 responses in COPD are associated with systemic inflammation

引用

Cellular & Molecular Immunology 2014年第6期11卷 606-608页

作者： Dino BA Tan Sonia Fernandez PatriciaPrice Martyn A French Philip J Thompson Yuben P Moodley centre for asthma Allergy and Respiratory Research Lung Institute of Western Australia Nedlands Australia School of Medicine and Pharmacology Nedlands Australia School of Pathology and Laboratory Medicine University of Western Australia Nedlands Australia Department of Clinical Immunology Perth Australia Department of respiratory and Sleep Medicine Royal Perth Hospital Perth Australia

Systemic inflammation is a feature of chronic obstructive pulmo- nary disease （COPD）. Defects in T cell-mediated anti-inflamma- tory pathways such as cytotoxic T lymphocyte antigen-4 （CTLA- 4） may promote damaging inflammation. This study provides novel data implicating the impaired induction of an anti-inflam- matory molecule, CTLA-4 in the elevated inflammation observed in COPD patients. Low induction of CTLA-4 in COPD patients paralleled increased markers of systemic inflammation ex vivo and increased T-cell responses to a bacterial superantigen, staphylo- coccal enterotoxin-B （SEB） in vitro. This mechanism may explain the increased inflammation in COPD patients.

关键词： Impaired responses associated

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Amplified fragment length polymorphism analysis on D5S436 locus and its application to linkage analysis in gene diagnosis of asthma

引用

Chinese Medical Journal 1999年第2期112卷 17-19页

作者：黄克武翁心植刘敬忠朱章菱肖白阎梅张洪玉王辰 Beijing respiratory disease Research Center Beijing Red Cross Chaoyang Hospital Beijing 100020 China.

Objectives To detect the polymorphism of D5S436 locus, and to carry out an amplified fragment length polymorphism (Amp FLP) genotyping linkage analysis in asthma *** Allele and genotype frequencies of the high polymorphic D5S436 locus were determined in 92 unrelated Chinese individuals by using polymerase chain reaction (PCR). The amplified fragments were separated by denaturing polyacrylamide gel electrophoresis and silver staining techniques. Results Eight alleles and 22 genotypes were observed in this population. The heterozygosity was 78.26%. The polymorphism information content (PIC) was 0.76. Amp FLP genotyping linkage analyses were carried out in four asthma families, demonstrating that the locus was inherited according to Mendel's law and had a clear *** The PIC of D5S436 is high in Chinese individuals, so it could be used as a genetic marker of asthma in Chinese and might be useful in the gene diagnosis of asthma.

关键词： asthma D5S436 locus gene linkage

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Kinetics of the accumulation of group 2 innate lymphoid cells in IL-33-induced and IL-25-induced murine models of asthma:a potential role for the chemokine CXCL16

引用

Cellular & Molecular Immunology 2019年第1期16卷 75-86页

作者： Yan Li Shihao Chen Yafei Chi Yiran Yang Xiwen Chen Huating Wang Zhe Lv Jingjing Wang Linjie Yuan Ping Huang Kewu Huang Chris JCorrigan Wei Wang Sun Ying Department of Immunology School of Basic Medical SciencesCapital Medical UniversityBeijingP.R.China Department of Laboratory Animal Sciences Capital Medical UniversityBeijingChina Department of respiratory and Critical Care Medicine Beijing Chao-Yang HospitalCapital Medical University and Beijing Institute of Respiratory MedicineBeijingChina Faculty of Life Sciences&Medicine School of Immunology and Microbial SciencesAsthma UK Centre in Allergic Mechanisms of AsthmaKing’s College LondonLondonUK

ILC2s are implicated in asthma pathogenesis, but little is known about the mechanisms underlying their accumulation in *** investigated the time course of ILC2 accumulation in different tissues in murine models of asthma induced by a serial per-nasalchallenge with ovalbumin (OVA), house dust mice (HDM), IL-25 and IL-33 and explored the potential roles of ILC2-attractingchemokines in this phenomenon. Flow cytometry was used to enumerate ILC2s at various time points. The effects of cytokines andchemokines on ILC2 migration were measured in vitro using a chemotaxis assay and in vivo using small animal imaging. Comparedwith saline and OVA challenge, both IL-25 and IL-33 challenge alone induced significant accumulation of ILC2s in the mediastinallymph nodes, lung tissue and bronchoalveolar lavage fluid of challenged animals, but with a distinct potency and kinetics. In vitro,IL-33 and CXCL16, but not IL-25 or CCL25, directly induced ILC2 migration. Small animal in vivo imaging further confirmed that asingle intranasal provocation with IL-33 or CXCL16 was sufficient to induce the accumulation of ILC2s in the lungs followinginjection via the tail vein. Moreover, IL-33-induced ILC2 migration involved the activation of ERK1/2, p38, Akt, JNK and NF-κB, whileCXCL16-induced ILC2 migration involved the activation of ERK1/2, p38 and Akt. These data support the hypothesis that epitheliumderived IL-25 and IL-33 induce lung accumulation of ILC2s, while IL-33 exerts a direct chemotactic effect in this process. AlthoughILC2s express the chemokine receptors CXCR6 and CCR9, only CXCL16, the ligand of CXCR6, exhibits a direct chemoattractanteffect.

关键词： ILC2s asthma Accumulation IL-33 IL-25 Chemokine

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

MBD2 promotes Th2 differentiation in ovalbumin-induced CD4+T cells

引用

BIOCELL 2023年第11期47卷 2495-2502页

作者： QILU PAN YAN JIANG LINQIAO LI XIAOJING DU QIAN HAN FEIXIANG LING ROU LI SHUYUAN CHU LIN MAI JIANWEI HUANG LIBING MA Department of respiratory and Critical Care Medicine Affiliated Hospital of Guilin Medical UniversityGuilin541001China Laboratory of respiratory disease Affiliated Hospital of Guilin Medical UniversityGuilin541001China

Introduction:Allergen-specific CD4+T cells play a central role in autoimmune disorders,allergies and asthma,with Th2-type immunity being the typical functional response of CD4+T *** study aimed to investigate the role of MBD2 in regulating Th2 cell ***:Splenic mononuclear cells were extracted from C57BL/6 mice,and CD4+T cells were isolated using magnetic beads and confirmed through flow *** was employed to construct MBD2-silenced CD4+T *** vitro experiments were performed to treat splenogenic mononuclear cells and CD4+T cells with Ovalbumin(OVA),and Th2 cell ratios and IL-4 levels were assessed using flow cytometry and ***:The purity of the isolated CD4+T cells was 95.73%,confirming successful isolation of primary CD4+T *** to the control group,the Th2 cell ratio exhibited an increase in the Th2-induced *** with 5-Aza(concentrations,1-100μM)promoted Th2 cell differentiation and increased IL-4 ***,when combined with Th2 induction and 10μM 5-Aza treatment,silencing MBD2 further amplified Th2 cell ratios and elevated IL-4 levels in cell ***,OVA(concentration,200μg/mL)induced the differentiation of CD4+T cells into Th2 cells and increased IL-4 ***,silencing MBD2 significantly increased the Th2 cell ratio and IL-4 levels in OVA-treated CD4+T ***:In summary,OVA promoted CD4+T cell differentiation into Th2 cells and enhanced IL-4 ***2 was identified as a mediator of Th2 cell differentiation in splenic-derived CD4+T cells,influenced by OVA or 5-Aza treatment.

关键词： 5-Aza MBD2 CD4+T cells Th2 cells Ovalbumin

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

POLYMORPHISM OF ANGIOTENSIN CONVERTING ENZYME GENE AND GENETIC SUSCEPTIBILITY TO asthma WITH FAMILIAL AGGREGATION

引用

Chinese Medical Sciences Journal 2000年第1期15卷 24-28页

作者：高金明林耀广肖毅徐凯峰许文兵朱元珏马毅白彦 Department of respiratory disease Peking Union Medical College Hospital PUMC & CAMS Beijing 100730.

Angiotensin converting enzyme (ACE) plays a key role in the metabolism of angiotensin Ⅱ (AT Ⅱ) and inactivation of bradykinins and tachykinins, which are potent bronchialconstrictors and mediators of inflammation asthma, and ACE is heavily expressed in the lungs. An insertion deletion (D/I) polymorphism of ACE gene has been shown to be associated with levels of ACE. We investigate whether the polymorphism of ACE gene is associated with asthma and bronchial responsiveness. Methods. A case control study was carried out in 50 asthmatics, 7 families with at least 2 asthmatic individuals, and 50 healthy subjects. The insertion/deletion (I/D) polymorphism of ACE gene was amplified by polymerase chain reaction (PCR). Methacholine brocho provocation and pulmonary function tests were performed in all asthmatics. Results. There was an higher gene frequency of DD genotype of ACE gene in asthmatic subjects and families individuals compared with healthy subjects (46%, 53% vs 16%, Pasthmatics carrying non DD alleles than patients with DD genotype (73 78% vs 56 56%, Pasthma. DD genotype of ACE might be a risk factor for the degree of airway obstruction, it could also be implicated in pathogenesis of bronchial hyperresponsiveness.

关键词： asthma genetics polymorphism angiotensin converting enzyme

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Management of severe asthma:from stepwise approach to therapy to treatable traits?

引用

Precision Clinical Medicine 2021年第4期4卷 293-296页

作者： Gang Wang Vanessa M.McDonald Peter G.Gibson Department of respiratory and Critical Care Medicine Clinical Research Center for Respiratory DiseaseWest China HospitalSichuan UniversityChengdu 610041China Laboratory of Pulmonary Immunology and Inflammation Frontiers Science Center for Disease-Related Molecular NetworkSichuan UniversityChengdu 610041China Department of respiratory and Sleep Medicine John Hunter HospitalHunter Medical Research InstituteNewcastle 2305Australia centre of Excellence in Severe asthma and Priority Research centre for asthma and respiratory disease the University of NewcastleNewcastle 2308Australia School of Nursing and Midwifery the University of NewcastleNewcastle 2308Austral

Dear Editor,asthma is a heterogeneous disease,usually characterized by chronic airway *** disease affects more than 300 million individualsworldwide,and poses a significant ongoing health burden,especially for patients with poorly controlled and severe asthma.1 The stepwise approach to therapy recommended in asthma management guidelines is effective.

关键词： asthma stepwise airway

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：