Interleukin-12 was not involved in promotion of T helper cell differentiation induced by theophylline

作     者：Jiong YANG1, Wei GUO, Han-xiang NIE, Su-ping HU Departmentof Respiratory Disease, Renmin Hospital, Wuhan University, Wuhan 430060, China 

作者机构：Department of Respiratory Disease Renmin Hospital Wuhan University Wuhan 430060 

出 版 物：《Acta Pharmacologica Sinica》 (中国药理学报(英文版))

年 卷 期：2004年第25卷第12期

页      面：100-104页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100706[医学-药理学] 1002[医学-临床医学] 100602[医学-中西医结合临床] 10[医学] 

主　　题：asthma theophylline helper inducer T lymphocytes Th1 cells Th2 cells interleukin-12 

摘      要：AIM: To investigate the effect of theophylline on the naive T cell differentiation and the probable role of interleukin- 12 (IL-12). METHODS: Naive cord blood T cells were treated with theophylline 10 mg/L for 3 d after stimulation with PHA 100 mg/L. Differentiation of Tcells was analyzed by flow cytometry. Theophylline 10 mg/L and IL-12- mAb 0.025 mg/L were added in cord blood mononuclear cell (CBMC) cultures primed with LPS 1 mg/L to detect the levels of IL-12 and IL-12P40. The whole blood cultures were obtained from twelve health volunteers with or without administration of theophylline (200 mg). Cytokines were measured by enzyme linked immuno-sorbent assay. RESULTS: Theophylline promoted T helper 1 (Th1) cells differentiation from naive T cells (21.9 %±10.3 % vs 9.4 %±5.6 %, P0.05), but had no significant effect on Th2 deviation. But theophylline inhibited the production of IL-12 and IL-12P40 by CBMC in vitro (28±6 ng/L vs 57±14 ng/L and 88±34 ng/L vs 214±82 ng/L, P0.01) and reduced IL-12 and IL-12P40 levels in whole blood cultures from healthy subjects (19±11 ng/L vs 31±15 ng/L and 92±13 ng/L vs 196±49 ng/L, P0.01). CONCLUSION: Theophylline promoted the differentiation of Th1 cells. IL- 12 seemed not to be involved in this process.