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Preparation of Red Component in Secondary Metabolites of Recombinant Hansenula anomala N6076 and Its GC-MS Detection

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Agricultural biotechnology 2012年第4期1卷 58-60页

作者： Yonghong FAN Xiang JIN Peihong MAO College of Life Science and Technology Xinjiang University center of Ion beam biotechnology College of Physics Science and TechnologyXinjiang University

[ Objective] This study aimed to investigate the preparation of red component in secondary metabolites of recombinant Hansenula anomala strain N6076 and its GC-MS detection. [ Method] Thin-layer chromatography method was applied for large-scale preparation of red component in the secondary metabolites of re- combinant H. anomala strain N6076. The red component was dissolved in anhydrous ethanol for GC-MS detection and chemical structure comparison in the data- base to identify its type. [ Resultl The red component is preliminarily identified as a quinane compound, while no compound with exactly the same structure as the red component has been found in WILEY, Nist and Nbs compound libraries. [ Conclusion] This study laid the foundation for further NMR structure identification of the obtained red component and investigation of the relationship between its structure and biolo#cal effects.

关键词： Recombinant yeast Metabolitcs Red component Thin-layer chromatography GC-MS

Inhibitory effect of gold nanoparticles conjugated with interferon gamma and methionine on breast cancer cell line

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Asian Pacific Journal of Tropical Biomedicine 2016年第2期6卷 173-178页

作者： Nastaran Mohseni Fatemeh Salehi Sarvestani Mehdi Shafiee Ardestani Fatemeh Kazemi-Lomedasht Masoud Ghorbani biotechnology Research center Biotechnology Department Venom and Biotherapeutics Molecules Laboratory Pasteur Institute of Iran Department of Biology Science and Research Branch Islamic Azad University Department of Radiopharmacy Faculty of Pharmacy Tehran University of Medical Sciences Research and Development Department Pasteur Institute of Iran Department of Virology University of Ottawa

Objective: To develop a gold nanoparticles complex conjugated with interferon-gamma(IFN-g) and methionine along with application of hyperthermia using near-infrared laser beams for the treatment of cancer ***: Gold nanorods(10 nm) were conjugated with IFN-g and methionine using carbodiimide family and characterized after purification by dialysis bags. Breast cancer cells were cultured and incubated with gold nanorods at different concentrations followed by irradiation with near-infrared laser beam. Samples were then evaluated for their viability in order to determine the effect of treatment and variables by MTT ***: Zetasizer results confirmed the conjugation of gold nanorods with methionine and IFN-g. The median percentage of cell viability in 0.30 mg/m L concentration of gold nanorods was 82%. The cell viability reached to 85% at the same concentration of gold nanorods, which existed in the assayed complex. The results of MTT assay showed that the 0.60 mg/m L concentration of gold nanoparticles complex was toxic on tumor cells(P < 0.05). After exposure to hyperthermia, the viability of cells at 6 min decreased to77% in 0.30 mg/m L concentration of gold nanorods ***: The size and concentration of gold nanorods was not cytotoxic. However,their presence during irradiation near-infrared laser increased the number of dead cells during the treatment of cells.

关键词： Gold nanorods Breast cancer cell line Interferon gamma Near-infrared laser

Deleterious effects of electron beam irradiation on development and reproduction of tomato/potato psyllids, Bactericera cockerelli

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Insect Science 2020年第6期27卷 1311-1321页

作者： Jiaxin Lei Jia Meng Ivy WChen Weining Cheng Andrea L.beam Md-Sajedul Islam Woodward D.Bailey Suresh Pillai Keyan Zhu-Salzman Department of Entomology Texas A&M UniversityCollege StationTXUSA Institute for Plant Genomics&biotechnology Texas A&M UniversityCollege StationTXUSA Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau Ministry of AgricultureNorthwest A&F UniversityYanglingShaanxiChina USDA-APHIS-PPQ-S&T-CPHST MiamiFLUSA National center for Electron beam Research Texas A&M UniversityCollege StationTXUSA

The potato/tomato psyllid Bactericera cockerelli causes serious damage to several solanaceous crops by direct feeding and vectoring Candidatus Liberibacter solanacearum,a bacterial *** beam(ebeam)irradiation is an environmentally friendly,chemical-free alternative method that is increasing in use for disinfestation of insect *** hypothesize that this irradiation technology will have detrimental effects on potato psyllid and thus impede its disease *** this end,we explored the effects of ebeam treatment ranging from 50 to 500 Gy on survival,development and reproduction of this *** on psyllids was apparently *** irradiated at 350 Gy,eggs could not hatch,1st instar nymphs failed to emerge,and although a small portion of irradiated 5th instar nymphs survived,the emerged adults were mostly *** in eclosed adults suggests harmful effects of ebeam on *** was seriously impaired when female psyllids were exposed to ebeam at the 5th instar nymphal or young adult stage,presumably due to inability to form *** addition,reciprocal crosses between irradiated and untreated psyllids indicated that female psyllids were more radiosensitive than males to *** together,these findings indicate that ebeam negatively impacted potato psyllid development and reproduction,which would inevitably compromise its disease transmission capacity.A dose of 350 Gy can be considered as a reference dose for effective control of potato psyllids.

关键词： Bactericera cockerelli development electron beam(ebeam) irradiation reproductive system

Micro and Nanofabrication methods to control cell-substrate interactions and cell behavior: A review from the tissue engineering perspective

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Bioactive Materials 2018年第3期3卷 355-369页

作者： Menekse Ermis Ezgi Antmen Vasif Hasirci BIOMATEN Middle East Technical University(METU)Center of Excellence in Biomaterials and Tissue EngineeringAnkaraTurkey METU Department of Biomedical EngineeringAnkaraTurkey METU Department of BiotechnologyAnkaraTurkey METU Department of Biological SciencesAnkaraTurkey

Cell-substrate interactions play a crucial role in the design of better biomaterials and integration ofimplants with the tissues. Adhesion is the binding process of the cells to the substrate through interactionsbetween the surface molecules of the cell membrane and the substrate. There are severalfactors that affect cell adhesion including substrate surface chemistry, topography, and stiffness. Thesefactors physically and chemically guide and influence the adhesion strength, spreading, shape and fate ofthe cell. Recently, technological advances enabled us to precisely engineer the geometry and chemistry ofsubstrate surfaces enabling the control of the interaction cells with the substrate. Some of the mostcommonly used surface engineering methods for eliciting the desired cellular responses on biomaterialsare photolithography, electron beam lithography, microcontact printing, and microfluidics. Thesemethods allow production of nano- and micron level substrate features that can control cell adhesion,migration, differentiation, shape of the cells and the nuclei as well as measurement of the forces involvedin such activities. This review aims to summarize the current techniques and associate these techniqueswith cellular responses in order to emphasize the effect of chemistry, dimensions, density and design ofsurface patterns on cell-substrate interactions. We conclude with future projections in the field of cellsubstrateinteractions in the hope of providing an outlook for the future studies.

关键词： Microfabrication Micropattern Cell-material interaction Differentiation Cell adhesion

Development of an active-site titrant for SARS-CoV-2 main protease as an indispensable tool for evaluating enzyme kinetics

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Acta Pharmaceutica Sinica B 2024年第5期14卷 2349-2357页

作者： Rabea Voget Julian Breidenbach Tobias Claff Alexandra Hingst Katharina Sylvester Christian Steinebach Lan Phuong Vu Renato H.Weiße Ulrike Bartz Norbert Sträter Christa E.Müller Michael Gütschow Pharmaceutical Institute Pharmaceutical&Medicinal ChemistryUniversity of BonnBonn 53121Germany Institute of Bioanalytical Chemistry Center for Biotechnology and BiomedicineLeipzig UniversityLeipzig 04103Germany Department of Natural Sciences University of Applied Sciences Bonn-Rhein-SiegRheinbach 53359Germany

A titrant for the SARS-CoV-2 main protease(M^(pro))was developed that enables,for the first time,the exact determination of the concentration of the enzymatically active M^(pro) by active-site *** covalent binding mode of the tetrapeptidic titrant was elucidated by the determination of the crystal structure of the enzyme–titrant *** fluorogenic substrates of M^(pro),including a prototypical,internally quenched Dabcyl-EDANS peptide,were compared in terms of solubility under typical assay *** exploiting the new titrant,key kinetic parameters for the M^(pro)-catalyzed cleavage of these substrates were determined.

关键词： COVID-19 SARS-CoV-2 Main protease Peptide nitriles Fluorogenic substrates Active-site titration X-ray crystallography Inner filter effect

Converting lateral scanning into axial focusing to speed up three-dimensional microscopy

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Light(Science & Applications) 2020年第1期9卷 429-440页

作者： Tonmoy Chakraborty Bingying Chen Stephan Daetwyler Bo-Jui Chang Oliver Vanderpoorten Etai Sapoznik Clemens F.Kaminski Tuomas P.J.Knowles Kevin M.Dean Reto Fiolka Department of Cell Biology UT Southwestern Medical CenterDallasTXUSA Department of Physics and Astronomy University of New MexicoAlbuquerqueNMUSA Department of Chemical Engineering and biotechnology University of CambridgeCambridge CB30ASUK Department of Chemistry University of CambridgeLensfield RoadCambridge CB21EWUK Lyda Hill Department of Bioinformatics UT Southwestern Medical CenterDallasTXUSA

In optical microscopy,the slow axial scanning rate of the objective or the sample has traditionally limited the speed of volumetric ***,by conjugating either a movable mirror to the image plane in a remote-focusing geometry or an electrically tuneable lens(ETL)to the back focal plane,rapid axial scanning has been ***,mechanical actuation of a mirror limits the axial scanning rate(usually only 10–100 Hz for piezoelectric or voice coil-based actuators),while ETLs introduce spherical and higher-order aberrations that prevent high-resolution *** an effort to overcome these limitations,we introduce a novel optical design that transforms a lateral-scan motion into a spherical aberration-free axial scan that can be used for high-resolution *** a galvanometric mirror,we scan a laser beam laterally in a remote-focusing arm,which is then back-reflected from different heights of a mirror in the image *** characterize the optical performance of this remote-focusing technique and use it to accelerate axially swept light-sheet microscopy by an order of magnitude,allowing the quantification of rapid vesicular dynamics in three *** also demonstrate resonant remote focusing at 12 kHz with a two-photon raster-scanning microscope,which allows rapid imaging of brain tissues and zebrafish cardiac dynamics with diffraction-limited resolution.

关键词： mirror axial image

Crystallization Behavior of Palm Kernel Oil Monitored by <i>In-Situ</i>Focused beam Reflectance Measurement (FBRM) and Particles Video Microscope (PVM) during Suspension Crystallization

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Advances in Materials Physics and Chemistry 2015年第1期5卷 31-38页

作者： Leping Dang Shuai Li Hong Zhang Yaobin Si Zhanzhong Wang School of Chemical Engineering and Technology Tianjin University Tianjin China Wilmar biotechnology Research & Development center Co. Ltd. Shanghai China

Crystallization behavior and kinetics study of palm kernel oil (PKO) were investigated using differential scanning calorimetry (DSC) by controlling cooling and reheating rate within a certain range of temperature. The evolution of morphology and particle counts was analyzed by focused beam reflectance measurement (FBRM) and particles video microscope (PVM) at the nucleation stage during suspension crystallization. The particle counts and morphological evolution from needle-like aggregations to amorphous form from PVM were observed during the initial crystallization stage, which meant that a phase transition was likely to occur. This work can give a better understanding of complicated fat system crystallization behavior and provide some critical instructions to control fractionation process.

关键词： Palm Kernel Oil Crystallization Behavior Crystal Morphology In-Situ Analysis

Hydroacoustic estimates of fish biomass and spatial distributions in shallow lakes

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Journal of Oceanology and Limnology 2018年第2期36卷 587-597页

作者： LIAN Yuxi HUANG Geng Matgorzata GODLEWSKA CAI Xingwei LI Chang YE Shaowen LIU Jiashou LI Zhongjie State Key Laboratory of Freshwater Ecology and biotechnology Institute of Hydrobiology Chinese Academy of Sciences Wuhan 430072 China Research center of Aquatic Organism Conservation and Water Ecosystem Restoration in Anhui Province Anqing Normal University Anqing 246133 China University of Chinese Academy of Sciences Beijing 100049 China Polish Academy of Sciences European Regional Centre for Ecohydrology ul. Tylna 3 90 36 4 Lodz Poland

We conducted acoustical surveys with a horizontal beam transducer to detect fish and with a vertical beam transducer to detect depth and macrophytes in two typical shallow lakes along the middle and lower reaches of the Changjiang(Yangtze) River in November 2013. Both lakes are subject to active fish management with annual stocking and removal of large fish. The purpose of the study was to compare hydroacoustic horizontal beam estimates with fish landings. The preliminary results show that the fish distribution patterns dif fered in the two lakes and were af fected by water depth and macrophyte coverage. The hydroacoustically estimated fish biomass matched the commercial catch very well in Niushan Lake, but it was two times higher in Kuilei Lake. However, acoustic estimates included all fish, whereas the catch included only fish >45 cm(smaller ones were released). We were unable to determine the proper regression between acoustic target strength and fish length for the dominant fish species in the two lakes.

关键词： horizontal hydroacoustics assessment of fish abundance fisheries management Chinese shallow lakes spatial distribution

Ultrafast laser system based on noncollinear optical parametric amplification for laser spectroscopy

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Chinese Optics Letters 2015年第12期13卷 32-35页

作者：韩冬佳李妍妍杜鹃王坤李永舫 Tomohiro Miyatake Hitoshi Tamiaki Takayoshi Kobayashi 冷雨欣 State Key Laboratory of High Field Laser Physics Shanghai Institute of Optics and Fine MechanicsChinese Academy of Sciences Laboratory of Advanced Optoelectronic Materials College of ChemistryChemical Engineering and Materials ScienceSoochow University Department of Materials Chemistry Ryukoku University Department of Bioscience and biotechnology Ritsumeikan University Advanced Ultrafast Laser Research center University of Electro-Communications FSA Collaborative Innovation center Shanghai Jiao Tong University

We report the experimental demonstration of transform-limited sub-6 fs pulses at an optimal central wavelength by a tunable noncollinear optical parametric amplification（NOPA） source. Meanwhile, a white light continuum in the near-infrared（NIR） range from 900 to 1100 nm is also successfully generated by focusing the unconverted800 nm beam during NOPA generation on a sapphire rod. Both visible-pump/visible-probe and visible-pump/NIR-probe experiments are realized using the same laser system. As examples, ultrafast photo-induced exciton dynamics inside two kinds of materials are investigated by the visible-pump/visible-probe and visible-pump/NIR-probe spectroscopy, respectively.

关键词： Band structure Infrared devices Laser spectroscopy Pumping (laser) Sapphire Ultrafast lasers