Effects of nitrogen ion irradiation on endoglucanase activity and gene mutation of Bacillus subtilis Bac01

作     者：LU Jie MAO Peihong JIN Xiang YU Long YING Hanjie 

作者机构：College of Biotechnology and Pharmaceutical Engineering Nanjing University of Technology 210009 Nanjing China Center oflon Beam Biotechnology College of Physics Science and Technology Xinjiang University 830008 Urumqi China 

出 版 物：《Nuclear Science and Techniques》 (核技术（英文）)

年 卷 期：2009年第20卷第5期

页      面：271-276页

核心收录：

学科分类：08[工学] 082701[工学-核能科学与工程] 0827[工学-核科学与技术] 

基　　金：Supported by the Natural Science Foundations of Xinjiang (No.2009211B06) Xinjiang University (No. QN070109) 

主　　题：放射性 能级 核技术 研究 氮离子 

摘      要：Bacillus subtilis Bac01 was mutated by 15 keV N+ ions of 1.5×1016 cm-2. The mutant strain Bac11 with high yield of endoglucanase was isolated using carboxymethylcellulose sodium and congo red indicative plates. It exhibited higher endoglucanase activitiy (381.89IU) than the original strain Bac01 (93.33IU). Two 1,500 bp endoglucanase gene fragments were obtained with PCR amplification from B. subtilis Bac01 and mutant strain Bac11. BLAST comparison result indicated that 10 nucleotides mutated. Bioinformatics methods were used to analyze the two predicted amino acid sequences, and it was found that 5 amino acid residues changed, being all in the cellulose-binding domain of endoglucanase.