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Expression of a High Mobility Group Protein Isolated from Cucumis sativus Affects the Germination of Arabidopsis thaliana under Abiotic Stress Conditions

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Journal of Integrative Plant Biology 2008年第5期50卷 593-600页

作者： Ji Young Jang Kyung Jin Kwak Hunseung Kang Department of Plant biotechnology Agricultural Plant Stress Research Center and Biotechnology Research InstituteCollege of Agriculture and Life SciencesChonnam National UniversityGwangju 500-757Korea

Although high mobility group B （HMGB） proteins have been identified from a variety of plant species, their importance and functional roles in plant responses to changing environmental conditions are largely unknown. Here, we investigated the functional roles of a CsHMGB isolated from cucumber （Cucumis sativus L.） in plant responses to environmental stimuli. Under normal growth conditions or when subjected to cold stress, no differences in plant growth were found between the wild-type and transgenic Arabidopsis thaliana overexpressing CsHMGB. By contrast, the transgenic Arabidopsis plants displayed retarded germination compared with the wild-type plants when grown under high salt or dehydration stress conditions. Germination of the transgenic plants was delayed by the addition of abscisic acid （ABA）, implying that CsHMGB affects germination through an ABA-dependent way. The expression of CsHMGB had affected only the germination stage, and CsHMGB did not affect the seedling growth of the transgenic plants under the stress conditions. The transcript levels of several germination-responsive genes were modulated by the expression of CsHMGB in Arabidopsis. Taken together, these results suggest that ectopic expression of a CsHMGB in Arabidopsis modulates the expression of several germinationresponsive genes, and thereby affects the germination of Arabidopsis plants under different stress conditions.

关键词： abiotic stress cucumber Cucumis sativus high mobility group protein transgenic Arabidopsis plant.

SSR Analysis of Genetic Relationship and Classification in Chrysanthemum Germplasm Collection

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Horticultural Plant Journal 2018年第2期4卷 73-82页

作者： LUO Chang CHEN Dongliang CHENG Xi LIU Hua LI Yahui HUANG Conglin Beijing Agro-biotechnology Research center Beijing Academy of Agriculture and Forestry Sciences Beijing Engineering Research Center of Functional Floriculture Beijing Key Laboratory of Agricultural Genetic Resources and Biotechnology

To further test the accuracy and rationality of the Chinese classification system for chrysanthemum(Chrysanthemum morifolium), 10 simple sequence repeat(SSR) markers were used to identify a collection of 88 chrysanthemum and its related genera accessions. In total, 42 effective alleles across 88 accessions were detected; 3 429 bands were obtained by PCR amplification, including 2 630 polymorphic bands. The similarity coefficient ranged from 0.53 to 0.88. Cluster analysis based on UPGMA illustrated that the wild species and large-flower cultivars were first divided into two clusters, then the large-flower cultivars formed five distinct groups according to petal type, indicated that petal type can be a classification criterion. In the wild species cluster, C. vestitum and C. zawadaskii grouped with A. trilobata, suggested that the Ajania genera was closely related to the Chrysanthemum genera. ‘Hangbaiju’, ‘Gongju’ and ‘Chuju’ were grouped together, and ‘Boju’, O. longilobus and C. mongolicum constituted another branch, showed a correlation with geographic region of origin. Population structure analysis was subsequently performed with K values ranging from 2 to 10, and the most likely estimate for the population structure is five subpopulations, which is nearly consistent with the clustering results. Principle component analysis was further performed to verify the classification results. The results showed that these SSR markers are very powerful for studying genetic relationships and will be useful tools in the identification and classification of chrysanthemum.

关键词： chrysanthemum SSR polymorphism genetic relationship classification

MONOCLONAL ANTIBODIES FOR DETECTING BACTERIOCIN M2 FROM STRAIN M2 OF Pseudomonas solanacearum

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Chinese Science Bulletin 1992年第1期37卷 56-60页

作者：李汝刚王远程蔡少华张向东谢道昕范云六 biotechnology Research center Chinese Academy of Agricultural Sciences Beijing 100081 PRC

Plant wilt bacteria (P. solanacearum)have a wide host range and cause wilt diseases of more than 200 species from 33 families. Because of no suitable resistant materials to wilt diseases, Fan Yun-liu et al. first proposed that this disease would be controlled with the genetic engineering of bacteriocin. Since bacteriocin M2 from strain M2 of P.

关键词： plant wilt bacteria bacteriocin hybridoma cell lines monoclonal antibodies.

EXPRESSION OF THE Bacillus thuringiensis SUBSP, KURSTAKI HD-1 δ-ENDOTOXIN GENE IN Escherichia coli

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Chinese Science Bulletin 1992年第2期37卷 146-149页

作者：陈骐范云六 biotechnology Research center Chinese Academy of Agricultural Sciences Beijing 100081 PRC

In 1987, Vaeck and Fischhoff reported the transformation of the B. thuringiensis (abbreviated to B. t. ) δ-endotoxin gene into tobacco and tomato respectively, and obtained the transgenic plants. Since then, more att... 详细信息

关键词： Bacillus thuringiensis δ-endotoxin gene expression.

Anticancer activity test of ethyl acetate extract of endophytic fungi isolated from soursop leaf(Annona muricata L.)

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Asian Pacific Journal of Tropical Medicine 2017年第6期10卷 635-640页

作者： Minarni I Made Artika Heddy Julistiono Nurliani Bermawie Eny Ida Riyanti Hasim Akhmad Endang Zainal Hasan Department of biochemistry Faculty of Mathematics and SciencesBogor Agricultural UniversityDarmaga CampusBogor 16680Indonesia Research center for Biology The Indonesian Institute of Sciences(LIPI)Cibinong-Bogor Indonesia Research Institute of Medicinal and Aromatic Plants Agricultural Research AgencyBogor-Indonesia center for biotechnology and Genetic Resources of Agriculture Agricultural Research AgencyBogor-Indonesia

Objective: To analyze anticancer activity of an ethyl acetate extract of endophytic fungi isolated from soursop leaf(Annona muricata L.). Methods: Anticancer activity of fungal extracts was determined by observing its toxicity against MCF-7(Michigan Cancer Foundation-7) cells in vitro by using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) assay method. At an extract concentration of 100 μg/m L, 4 isolates out of 12 showed high activity against the cancer cell growth. The four isolates were then selected for further IC50 determination, by measuring the inhibition of cancer cell proliferation at extract concentration of 25 μg/m L, 50 μg/m L, 100 μg/m L, 200 μg/m L and 400 μg/m L. Results: Results showed that isolate Sir-G5 had the highest anticancer activity with an IC50 of 19.20 μg/m L. The best isolates were screened again using a normal cell(Chang cells) to determine its toxicity against normal cells. Results indicated that the extracts do not affect the proliferation of normal cells. Molecular identification showed that the fungal isolate Sir-G5 has a close relationship with Phomopsis sp. Conclusions: The endophytic fungi isolated from soursop leaf has the potential to be used as a source of anticancer agents.

关键词： Annona muricata L Anticancer MCF-7 Endophytic fungi Soursop leaf

Expressed sequence tags (ESTs) analysis of the ripening Vitis amurensis cv. Shuang Hong berry skins

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Journal of Forestry Research 2013年第3期24卷 495-502页

作者： JI Xiang-Nan LI Feng YANG Cheng-Jun LI Bo WANG Jun ZHANG Wen center for Viticulture and Enology College of Food Science & Nutritional Engineering China Agricultural University College of Forestry Northeast Forestry University College of Agriculture & biotechnology China Agricultural University

Vitis amurensis is a valuable resource for wine production. Ripening of the grape berry is the key phase which determines the com- position of wine. To better understand the gene expression that manifest in V. amurensis berry skins during the ripening, cDNA library of V. amurensis berry skins was constructed. A total of 935 high quality ex- pressed sequence tags （ESTs） were obtained from the library. These ESTs represent 636 unigenes, including 108 contigs and 528 singletons. The EST analysis was performed and genes were assigned to functional categories according to their primary BLAST match. Of these 25.35% were involved with metabolism, 6.27% with cell rescue and defense, 6.84% energy, 11.68% protein synthesis, 18.8% protein activity regula- tion, 11.11% cell structure, 7.98% transport, 6.27% transcription and the remaining 5.7% were signal transduction. The generated ESTs were characterized by the gene ontology analysis and were categorized ac- cording to its cellular component, molecular function and biological process. In the cDNA library, some genes are relevant to the biosynthesis of anthocyanins, while some genes are related to grape berry maturation.

关键词： Vitis amurensis expressed sequence tags (ESTs) cDNAlibrary unigene annotation gene ontology

RAPD marker linked with sbm-1, a gene conferring resistance to pea seed-born mosaic virus

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Chinese Science Bulletin 1996年第21期41卷 1831-1834页

作者：李汝刚范云六 N.F.Weeden biotechnology Research center Chinese Academy of Agricultural Sciences Beijing 100081 China Department of Horticulture Cornell UniversityUSA

DNA markers linked with trait of disease-resistance offer a very useful tool forselection of putative disease-resistant progeny and cloning disease-resistant ***-born mosaic virus(PSbMV)is spread mainly through planting infected pea seeds andby non-persistent aphid *** strains of PSbMV have been identified,designated as P-1,L and *** P-1 is most widely *** recessive

关键词： pea pea seed-born mosaic virus RAPD.

Investigating Interaction Between Biochanin A and Human Serum Albumin by Multi-spectroscopic and Molecular Simulation Methods

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Transactions of Tianjin University 2017年第4期23卷 325-333页

作者： Zhaohui Xue Aiqing Cheng Yanni Li Wancong Yu Xiaohong Kou School of Chemical Engineering and Technology Tianjin University Medical Plant Lab Tianjin Research Center of Agricultural Biotechnology

Biochanin A (BCA), the most abundant isoflavone in chickpeas, presents a wide range of biological activities, such as hypolipidaemic, anti-oxidative, anti-proliferative, and estrogen-like effects. We investigated the interaction between BCA and human serum albumin (HSA) via several techniques. UV–Vis absorption spectroscopy verified the conformational variation of HSA after BCA addition, and fluorescence spectroscopy revealed the relevant binding parameters. Circular dichroism spectroscopy was used to estimate the secondary structural changes of HSA with and without BCA. Molecular docking and dynamics simulations were then applied to study the characteristics of HSA with BCA. Energy decomposition analysis was used to prove that Trp214 in subdomain IIA of HSA is the most likely binding site of BCA. Van der Waals forces and hydrophobic interactions may play important roles during the binding process. All of our results showed that BCA presents significant binding affinity to HSA, thus confirming that the role of HSA has as an efficient transporter of biomolecules. © 2017, Tianjin University and Springer-Verlag Berlin Heidelberg.

关键词： Absorption spectroscopy Binding energy Bins Body fluids Dichroism Flavonoids Fluorescence spectroscopy Hydrophobicity Molecular biology Molecular dynamics Van der Waals forces

Biochanin A and CPe-Ⅲ Peptide Improved Hepatic Infl ammation by Regulating the Hepatic Lipid Metabolic Pathways in Diet-Induced Obese Mice

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Transactions of Tianjin University 2018年第3期24卷 234-243页

作者： Zhaohui Xue Ru Wang Haichao Wen Wancong Yu Xiaohong Kou School of Chemical Engineering and Technology Tianjin University Medical Plant Laboratory Tianjin Research Center of Agricultural Biotechnology

Biochanin A(BCA) and CPe-Ⅲ peptide, which both exist in chickpea(Cicer arietinum L.), possess significant antihyperlipidemic properties. However, the actualmechanisms ofthose compounds in inhibiting the dysregulation oflipid metabolism and complicated inflammation have not been wellcharacterized. This study investigated the effects ofBCA, CPe-Ⅲ peptide, and combined BCA and CPe-Ⅲ peptide(BC) on the expression ofgenes involved in hepatic lipid and inflammation metabolism. Results demonstrated that BCA, CPe-Ⅲ peptide, and BC significantly attenuated hepatitis and hyperlipidemia by downregulating those genes involved in pro-inflammatory cytokines(TNF-α), hepatic fatty acid(FA) synthesis(ACC1 and FAS), cholesterolmetabolism(SREBP2, HMGCR, and PCSK9), and upregulating key regulators involved in FA oxidation(PPARα and FABP1), lipolysis(ATGL), LDLR, reverse cholesteroltransport(ABCA1, SR-B1, and LXRα), and cholesterolcatabolism(CYP7 A1). Moreover, they also altered the expression oflipid metabolism-related proteins, including SREBP2, PCSK9, LDLR, ABCA1, and CYP7 A1. Finally, these results revealed that the combination treatment ofBCA and CPe-Ⅲ peptide resulted in greater antihyperlipidemic activity compared with individualcompounds.

关键词： Biochanin A CPe-Ⅲ peptide Lipid metabolism Infl ammation Gene expression Protein expression