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Stability and transepithelial transport of oligopeptide(KRQKYD)with hepatocyte-protective activity from Jinhua ham in human intestinal Caco-2 monolayer cells

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food Science and Human Wellness 2024年第3期13卷 1503-1512页

作者： Wen Nie Feiran Xu Kai Zhou Jieying Deng Ying Wang Baocai Xu Engineering research center of Bio-process Ministry of EducationSchool of Food and Biological EngineeringHefei University of TechnologyHefei 230601China School of Biological and food Engineering Chuzhou UniversityChuzhou 239001China

The study evaluated the stability of an oligopeptide(Lys-Arg-Gln-Lys-Tyr-Asp,KRQKYD)and its transport mechanism by simulating gastrointestinal digestion and a model of human intestinal Caco-2 monolayer cells in *** this study,the effects of environmental factors(temperature,pH and NaCl concentration)and simulated gastrointestinal digestion on the stability of KRQKYD were evaluated by indicators of the levels of alanine transaminase(ALT),aspartate transaminase(AST)and malondialdehyde(MDA)in an alcoholinduced hepatocyte injury *** results showed that KRQKYD still maintained satisfactory hepatocyteprotective activity after treatment with different temperatures(20-80℃),pH(3.0-9.0),NaCl concentration(1%-7%)and simulated gastrointestinal digestion,which indicated that KRQKYD showed good stability to environmental factors and simulated gastrointestinal ***,the intact KRQKYD could be absorbed in a model of Caco-2 monolayer cells with a P_(app)value of(9.70±0.53)×10^(-7)cm/*** with an energy inhibitor(sodium azide),a competitive peptide transporter inhibitor(Gly-Pro)and a transcytosis inhibitor wortmannin did not decrease the level of transepithelial KRQKYD transport,indicating that the transport mechanism of KRQKYD was not associated with energy dependent,vector mediated and *** tight junction disruptor cytochalasin D significantly increased the level of transepithelial KRQKYD transport(P<0.05),suggesting that intact KRQKYD was absorbed by paracellular transport.

关键词： Jinhua ham KRQKYD(Lys-Arg-Gln-Lys-Tyr-Asp) Environmental stability Gastrointestinal digestive Transport mechanism

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The Burden of Hypertension-Related Chronic Kidney Disease—China,2010–2019

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China CDC weekly 2024年第14期6卷 282-288页

作者： Youyuan Bu Yueru Liu Maigeng Zhou Peng Yin Kejun Liu Yamin Bai Xuancheng Lu Chinese center for Disease Control and Prevention BeijingChina National center for Chronic and Non-Communicable Disease Control and Prevention Chinese Center For Disease Control and PreventionBeijingChina China National Health development research center BeijingChina

What is already known about this topic?The global burden of chronic kidney disease(CKD)is on the *** is added by this report?In 2019,5.58 million individuals in China were affected by CKD related to hypertension,leading to 70,260 fatalities and 1.69 million disability-adjusted life years(DALYs).The most affected groups were men,older individuals,and residents of western *** the period from 2010–2019,the age-standardized prevalence rate(ASPR)remained constant,and the agestandardized mortality rate(ASMR)and agestandardized DALY rate(ASDR)showed a decreasing ***,there was an increase in the number of cases,deaths,and DALYs associated with this *** are the implications for public health practice?Hypertension significantly contributes to the burden of CKD;therefore,raising awareness and implementing early screening measures are essential.

关键词： Kidney mortality burden

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Aerodynamic/stealth design of S-duct inlet based on discrete adjoint method

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Applied Mathematics and Mechanics(English Edition) 2024年第4期45卷 725-746页

作者： Jun DENG Ke ZHAO Lin ZHOU Wei ZHANG Bowen SHU Jiangtao HUANG Zhenghong GAO School of Aeronautics Northwestern Polytechnical UniversityXi’an 710072China Aerospace Technology Institute of China Aerodynamic Research and Development CenterMianyang 621000Sichuan ProvinceChina

It is a major challenge for the airframe-inlet design of modern combat aircrafts,as the flow and electromagnetic wave propagation in the inlet of stealth aircraft are very *** this study,an aerodynamic/stealth optimization design method for an S-duct inlet is *** upwind scheme is introduced to the aerodynamic adjoint equation to resolve the shock wave and flow *** multilevel fast multipole algorithm(MLFMA)is utilized for the stealth adjoint equation.A dorsal S-duct inlet of flying wing layout is optimized to improve the aerodynamic and stealth *** the aerodynamic and stealth characteristics of the inlet are effectively ***,the optimization results are analyzed,and it shows that the main contradiction between aerodynamic characteristics and stealth characteristics is the centerline and crosssectional *** S-duct is smoothed,and the cross-sectional area is increased to improve the aerodynamic characteristics,while it is completely opposite for the stealth *** radar cross section(RCS)is reduced by phase cancelation for low frequency *** method is suitable for the aerodynamic/stealth design of the aircraft airframe-inlet system.

关键词： S-duct inlet aerodynamic/stealth optimization design discrete adjoint upwind scheme multilevel fast multipole algorithm(MLFMA)

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research on organized method and regulatory mechanism of Cell cultured meat based on modified silk

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food Science and Human Wellness 2024年

作者： Feng Yang Haijuan Hu Yingying Li Shilei Li Wenting Liu Yu Qi Shouwei Wang China Meat research center Beijing Institute of food Science

Cell cultured meat has been extensively studied as an environmentally friendly， energy-saving and more effective technology. However， there are many technical bottlenecks， especially the regulatory mechanism and manufacturing method of in vitro myogenesis. Based on an edible modified silk protein scaffold， with 3D culturing， in situ differentiated and transcriptome analysis， this study describes novel scaffolds and fabrication methods for cell cultured meat. The results showed that the effective space and utilization efficiency for cell culture of the scaffold is 26-1000 that of the traditional culture dish; it could form a tissue-like structure. Transcriptomics revealed the regulatory pathways and key factors of different cycles. It clarifies that the multi-cycle process of myoblast myogenesis in vitro is different from the single feedback regulation in vivo. More importantly， a novel scaffold-based cell cultured meat manufacturing method was developed， further develop a new tissue culture solution that is different from existing cell culture meat *** manufacturing processes， it provides a new cell culture meat technology system， provides a theoretical basis for the regulation of cell proliferation and muscle growth， and lays the technical foundation for in situ tissue culture of cell cultured meat in vitro.

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An anti-icing scaling method for wind tunnel tests of aircraft thermal ice protection system

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Chinese Journal of Aeronautics 2024年第6期37卷 1-6页

作者： Yu LIU Qiang WANG Xian YI Ningli CHEN Jinghao REN Weihao LI Key Laboratory of Icing and Anti/de-icing China Aerodynamics Research and Development CenterMianyang 621000China State Key Laboratory of Aerodynamics Mianyang 621000China

The efficiency of the aircraft Ice Protection Systems(IPSs)needs to be verified through icing wind tunnel ***,the scaling method for testing the IPSs has not been systematically established yet,and further research is *** the present study,a scaling method specifically designed for thermal IPSs was derived from the governing equation of thin water *** scaling parameters were adopted to address the heat and mass transfer involved in the thermal anti-icing *** method validation,icing wind tunnel tests were conducted using a jet engine nacelle model equipped with a bleed air *** non-dimensional surface temperature and runback ice closely matched for both the reference and scaled *** validation confirms that the scaling method is capable of achieving the similarity of surface temperature and the runback ice *** anti-icing scaling method can serve as an important supplement to the existing icing similarity theory.

关键词： Scaling method Ice protection system Water film Anti-icing test Icing wind tunnel

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Efficient biosynthesis of creatine by whole-cell catalysis from guanidinoacetic acid in Corynebacterium glutamicum

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Synthetic and Systems Biotechnology 2024年第1期9卷 99-107页

作者： Chunjian Li Pengdong Sun Guoqing Wei Yuqi Zhu Jingyuan Li Yanfeng Liu Jian Chen Yang Deng College of food Science and Engineering Qingdao Agricultural UniversityQingdao 266109China Key Laboratory of Special food Processing(Co-construction by Ministry and Province) Ministry of Agriculture Rural AffairsQingdao Agricultural UniversityQingdao 266109China Shandong Technology Innovation center of Special food Qingdao 266109China Qingdao Special food research Institute Qingdao 266109China Qingdao Nuoan Baite Biotechnology Co. Ltd.Qingdao 266109China Science center for Future foods Engineering Research Center of Ministry of Education on Food Synthetic Biotechnologyand Jiangsu Province Engineering Research Center of Food Synthetic BiotechnologyJiangnan UniversityWuxi 214122China Key Laboratory of Carbohydrate Chemistry and Biotechnology Ministry of EducationSchool of BiotechnologyJiangnan UniversityWuxi 214122China

Creatine is a naturally occurring derivative of an amino acid commonly utilized in functional foods and ***,the current industrial synthesis of creatine relies on chemical processes,which may hinder its utilization in certain ***,a biological approach was devised that employs whole-cell biocatalysis in the bacterium Corynebacterium glutamicum,which is considered safe for use in food production,to produce safe-for-consumption *** objective of this study was to identify a guanidinoacetate N-methyltransferase(GAMT)with superior catalytic activity for creatine *** employing whole-cell biocatalysis,a gamt gene from Mus caroli(Mcgamt)was cloned and expressed in *** ATCC 13032,resulting in a creatine titer of 3.37 g/***,the study employed a promoter screening strategy that utilized nine native strong promoters in *** to enhance the expression level of *** highest titer was achieved using the P1676 promoter,reaching 4.14 g/*** conditions of whole-cell biocatalysis were further optimized,resulting in a creatine titer of 5.42 g/*** is the first report of successful secretory creatine expression in ***,which provides a safer and eco-friendly approach for the industrial production of creatine.

关键词： Creatine Corynebacterium glutamicum Whole-cell biocatalysis Guanidinoacetate N-Methyltransferase food additive

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Bifidobacterium breve CCFM1078 regulates bone development in growing BALB/c mice via gut microbiota and metabolites to balance bone formation and resorption

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food Science and Human Wellness 2024年

作者： Bowen Li Mengfan Ding Xiaoming Liu Haiqin Chen Jianxin Zhao R.Paul Ross Catherine Stanton Shilong Jiang Wei Chen Bo Yang Nutrition and Metabolism research Division， Innovation center， Heilongjiang Feihe Dairy Co.， Ltd. National Engineering research center for Functional food， Jiangnan University State Key Laboratory of food Science and Technology， Jiangnan University PKUHSC-China Feihe Joint research Institute of Nutrition and Healthy Lifespan development APC Microbiome Ireland， University College Cork School of food Science and Technology， Jiangnan University International Joint research center for Probiotics & Gut Health， Jiangnan University Teagasc food research Centre

This study endeavors to investigate the effects of Bifidobacterium breve CCFM1078 on bone formation and resorption balance in growing BALB/c mice. Newborn BALB/c mice were assigned to the control group (administration saline) and the CCFM1078 group (administration B. breve CCFM1078， 3 × 109CFU/day) in 3-， 4-， and 5-week tests. All the groups have male and female distinctions. Our findings demonstrate that B. breve CCFM1078 exerts on the dynamic equilibrium between bone formation and resorption during the critical period of growth in mice by modulating the composition of gut microbiota and metabolites (hexadecanamide， linoleoyl ethanolamide， and palmitoyl ethanolamide)， the genes and proteins expression related to the GH/IGF-1 axis and Gs/PKA/CREB signaling pathways， as well as downstream osteogenic and osteoclastic differentiation factors. The effects of B. breve CCFM1078 were different with age and gender dependent. This finding suggests B. breve CCFM1078 may have potential applications in regulating bone metabolism in the growth period population.

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Neuropeptide Y receptor Y8b(npy8br)regulates feeding and digestion in Japanese medaka(Oryzias latipes)larvae:evidence from gene knockout

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2024年第7期25卷 605-616页

作者： Xiaodan JIA Ke LU Xufang LIANG College of Fisheries Chinese Perch Research CenterHuazhong Agricultural UniversityWuhan 430070China Engineering research center of Green development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt Ministry of EducationWuhan 430070China

Neuropeptide Y receptor Y8(NPY8R)is a fish-specific receptor with two subtypes,NPY8AR and *** in expression levels during physiological processes or in vivo regulation after ventricular injection suggest that NPY8BR plays an important role in feeding regulation;this has been found in only a few fish,at *** order to better understand the physiological function of npy8br,especially in digestion,we used clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9)technology to generate npy8br-/-japanese medaka(Oryzias latipes).We found that the deletion of npy8br in medaka larvae affected their feeding and digestion ability,ultimately affecting their ***,npy8br deficiency in medaka larvae resulted in decreased feed intake and decreased expression levels of orexigenic genes(npy and agrp).npy8br-/-medaka larvae fed for 10 d(10th day of feeding)still had incompletely digested brine shrimp(Artemia nauplii)in the digestive tract 8 h after feeding,the messenger RNA(mRNA)expression levels of digestion-related genes(amy,lpl,ctra,and ctrb)were significantly decreased,and the activity of amylase,trypsin,and lipase also significantly *** deletion of npy8br in medaka larvae inhibited the growth and significantly decreased the expression of growth-related genes(gh and igf1).Hematoxylin and eosin(H&E)sections of intestinal tissue showed that npy8br-/-medaka larvae had damaged intestine,thinned intestinal wall,and shortened intestinal *** far,this is the first npy8br gene knockout model established in fish and the first demonstration that npy8br plays an important role in digestion.

关键词： Neuropeptide Y receptor Y8b(npy8br) Japanese medaka(Oryzias latipes) Knockout Feeding Digestion

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A machine learning framework for remaining useful lifetime prediction of li-ion batteries using diverse neural networks

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Energy and AI 2024年第1期15卷 102-135页

作者： Junghwan Lee Huanli Sun Yongshan Liu Xue Li First Automobile Works Group Corporation research and development center No.1 Xinhongqi StreetChangchun130013JilinChina

Accurate prediction of the remaining useful life(RUL)of lithium-ion batteries(LIBs)is pivotal for enhancing their operational efficiency and safety in diverse *** operational advantages,precise RUL predictions can also expedite advancements in cell design and fast-charging methodologies,thereby reducing cycle testing *** artificial neural networks(ANNs)showing promise in this domain,determining the best-fit architecture across varied datasets and optimization approaches remains *** study introduces a machine learning framework for systematically evaluating multiple ANN *** only 30%of a training dataset derived from 124 LIBs subjected to various charging regimes,an extensive evaluation is conducted across 7 ANN *** architecture is optimized in terms of hyperparameters using this framework,a process that spans 145 days on an NVIDIA GeForce RTX 4090 *** optimizing each model to its best configuration,a fair and standardized basis for comparing their RUL predictions is *** research also examines the impact of different cycling windows on predictive *** a stratified partitioning technique underscores the significance of consistent dataset representation across ***,using only the features derived from individual charge–discharge cycles,our top-performing model,based on data from just 40 cycles,achieves a mean absolute percentage error of 10.7%.

关键词： Remaining useful life Artificial neural networks Li-ion batteries Deep neural networks Machine learning Hyperparameter optimization

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Exploration of cyclooxygenase-2 inhibitory peptides from walnut dreg proteins based on in silico and in vitro analysis

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food Science and Human Wellness 2024年第3期13卷 1636-1644页

作者： Zishan Hong Jing Xie Liang Tao Jing-Jing Dai Tingting Li Li Zhang Yuying Bai Xia Hu Jinlian Chen Jun Sheng Yang Tian College of food Science and Technology Yunnan Agricultural UniversityKunming 650201China Yunnan Provincial Key Laboratory of Precision Nutrition and Personalized food Manufacturing Yunnan Agricultural UniversityKunming 650201China Engineering research center of development and Utilization of food and Drug Homologous Resources Ministry of EducationYunnan Agricultural UniversityKunming 650201China School of Tea and Coffee Pu’er UniversityPu’er 665000China Yunnan Provincial Engineering research center for Edible and Medicinal Homologous Functional food Yunnan Agricultural UniversityKunming 650201China Key Laboratory of Pu-er Tea Science Ministry of EducationYunnan Agricultural UniversityKunming 650201China

Walnut dreg protein hydrolysates(WDPHs)exhibit a variety of biological activities,however,the cyclooxygenase-2(COX-2)inhibitory peptide of WDPHs remain *** aim of this study was to rapidly screen for such peptides in WDPHs through a combination of in silico and in vitro *** total,1262 peptide sequences were observed by nano liquid chromatography/tandem mass spectrometry(nano LC-MS/MS)and 4 novel COX-2 inhibitory peptides(AGFP,FPGA,LFPD,and VGFP)were *** kinetic data indicated that AGFP,FPGA,and LFPD displayed mixed-type COX-2 inhibition,whereas VGFP was a non-competitive *** is mainly because the peptides form hydrogen bonds and hydrophobic interactions with residues in the COX-2 active *** results demonstrate that computer analysis combined with in vitro evaluation allows for rapid screening of COX-2 inhibitory peptides in walnut protein dregs.

关键词： Walnut dreg proteins Cyclooxygenase-2 inhibitory peptide Identification Virtual screening Molecular docking

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