检索结果-南通市图书馆

Insights gained from single-cell analysis of chimeric antigen receptor T-cell immunotherapy in cancer

Military Medical Research 2024年第5期11卷 717-746页

作者： Lu Tang Zhong-Pei Huang Heng Mei Yu Hu Institute of Hematology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430022China Hubei Clinical Medical Center of cell therapy for Neoplastic Disease Wuhan430022China Key Laboratory of Biological Targeted therapy The Ministry of EducationWuhan430022China Hubei Key Laboratory of Biological Targeted therapy Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430022China

Advances in chimeric antigen receptor(CAR)-T cell therapy have significantly improved clinical outcomes of patients with relapsed or refractory hematologic ***,progress is still hindered as clinical benefit is only available for a fraction of patients.A lack of understanding of CAR-T cell behaviors in vivo at the single-cell level impedes their more extensive application in clinical *** evidence suggests that single-cell sequencing techniques can help perfect the receptor design,guide gene-based T cell modification,and optimize the CAR-T manufacturing conditions,and all of them are essential for long-term immunosurveillance and more favorable clinical *** information generated by employing these methods also potentially informs our understanding of the numerous complex factors that dictate therapeutic efficacy and *** this review,we discuss the reasons why CAR-T immunotherapy fails in clinical practice and what this field has learned since the milestone of single-cell sequencing *** further outline recent advances in the application of single-cell analyses in CAR-T ***,we provide an overview of single-cell studies focusing on target antigens,CAR-transgene integration,and preclinical research and clinical applications,and then discuss how it will affect the future of CAR-T cell therapy.

关键词： Single-cell sequencing Cancer immunotherapy CAR-T therapy cell heterogeneity Trajectory inference Tumor microenvironment

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Monitoring checkpoint inhibitors:predictive biomarkers in immunotherapy

引用

Frontiers of Medicine 2019年第1期13卷 32-44页

作者： Min Zhang Jingwen Yang Wenjing Hua Zhong Li Zenghui Xu Qijun Qian ShangHai cell therapy Group Co. Ltd.Shanghai 201805China

Immunotherapy has become the fourth cancer therapy after surgery, chemotherapy, and radiotherapy. In particular, immune checkpoint inhibitors are proved to be unprecedentedly in increasing the overall survival rates of patients with refractory cancers, such as advanced melanoma, non-small cell lung cancer, and renal cell carcinoma. However, inhibitor therapies are only effective in a small proportion of patients with problems, such as side effects and high costs. Therefore, doctors urgently need reliable predictive biomarkers for checkpoint inhibitor therapies to choose the optimal therapies. Here, we review the biomarkers that can serve as potential predictors of the outcomes of immune checkpoint inhibitor treatment, including tumor-specific profiles and tumor microenvironment evaluation and other factors.

关键词： immune checkpoint companion diagnosis PD-L1 tumor mutation burden immune score

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Antiviral therapy of hepatitis C as curative treatment of indolent B-cell lymphoma

引用

World Journal of Gastroenterology 2016年第38期22卷 8447-8458页

作者： Michele Merli Giuseppe Carli Luca Arcaini Carlo Visco Division of Hematology University Hospital Ospedale di Circolo & Fondazione MacchiUniversity of Insubria Department of cell therapy and Hematology San Bortolo Hospital Department of Molecular Medicine University of Pavia

The association of hepatitis C virus(HCV) and B-cell non-Hodgkin lymphomas(NHL) has been highlighted by several epidemiological and biological insights; however the most convincing evidence is represented by interventional studies demonstrating the capability of antiviral treatment(AT) with interferon(IFN) with or without ribavirin to induce the regression of indolent lymphomas, especially of marginal-zone origin. In the largest published retrospective study(100 patients) the overall response rate(ORR) after first-line IFN-based AT was 77%(44% complete responses) and responses were sustainable(median duration of response 33 mo). These results were confirmed by a recent metaanalysis on 254 patients, demonstrating an ORR of 73%. Moreover this analysis confirmed the highly significant correlation between the achievement of viral eradication sustained virological response(SVR) and hematological responses. Two large prospective studies demonstrated that AT is associated with improved survival and argue in favor of current guidelines' recommendation of AT as preferential first-line option in asymptomatic patients with HCV-associated indolent NHL. The recently approved direct-acting antiviral agents(DAAs) revolutionized the treatment of HCV infection, leading to SVR approaching 100% in all genotypes. Very preliminary data of IFN-free DAAs therapy in indolent HCV-positive NHL seem to confirm their activity in inducing lymphoma regression.

关键词： Non-hodgkin lymphomas Hepatitis C virus Antiviral therapy Interferon Ribavirin Sofosbuvir Direct-acting antiviral agents Marginal zone lymphomas

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Intelligent tunable CAR-T cell therapy leads the new trend

引用

Synthetic and Systems Biotechnology 2023年第4期8卷 606-609页

作者： Jiayi Cheng Mingyao Liu Jiqin Zhang Shanghai Frontiers Science Center of Genome Editing and cell therapy Shanghai Key Laboratory of Regulatory BiologyInstitute of Biomedical Sciences and School of Life SciencesEast China Normal UniversityShanghaiChina

Adoptive transfer of T cells engineered with chimeric antigen receptor(CAR)has been proved to have robust anti-tumor effects against hematological ***,problems about safety and efficacy,such as cytokine release syndrome(CRS),T cell exhaustion and antigen escape are still raised when patients are treated with CAR-T ***,CAR-T therapy has limited applications in treating solid tumors,owing to inefficient infiltration and poor functional persistence of CAR-T cells and diverse immunosuppression in tumor *** order to overcome these limitations and broad its applications,multiple controllable CAR-T technologies were *** this article,we review the designs of intelligent controlled CAR-T technologies and the innovations that they bring about in recent years.

关键词： Chimeric antigen receptor T Tumor Safety and efficacy On and off systems Autologous universal system Synthetic notch system

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Hepato-biliary profile of potential candidate liver progenitor cells from healthy rat liver

引用

World Journal of Gastroenterology 2012年第27期18卷 3511-3519页

作者： Cédric Maerckx Isabelle Scheers Tatiana Tondreau David Campard Omar Nyabi Mustapha Najimi Etienne Sokal Laboratory of Pediatric Hepatology and cell therapy Université Catholique de Louvain1200 BrusselsBelgium

AIM： To evaluate the presence of progenitor cells in healthy adult rat liver displaying the equivalent ad- vanced hepatogenic profile as that obtained in humans. METHODS： Rat fibroblastic-like liver derived cells （rFLDC） were obtained from collagenase-isolated liver cell suspensions and characterized and their phenotype profile determined using flow cytometry, immunocyto- chemistry, reverse transcription polymerase chain reac- tion and functional assays. RESULTS： rFLDC exhibit fibroblastoid morphology, ex- press mesenchymal （CD73, CD90, vimentin, m-smooth muscle actin）, hepatocyte （UGTIA1, CK8） and biliary （CK19） markers. Moreover, these cells are able to store glycogen, and have glucose 6 phosphatase activity, but not UGTIA1 activity. Under the hepatogenic differentia- tion protocol, rFLDC display an up-regulation of hepatocyte markers expression （albumin, tryptophan 2,3-di- oxygenase, G6Pase） correlated to a down-regulation of the expression of the biliary marker CK19. CONCLUSION： Advanced hepatic features observed in human liver progenitor cells could not be demonstrated in rFLDC. However, we demonstrated the presence of an original rodent hepato-biliary cell type.

关键词： Hepato biliary profile Hepatogenic differentiation Liver Progenitor cell Rat

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Labeling of cynomolgus monkey bone marrow-derived mesenchymal stem cells for cell tracking by multimodality imaging

引用

Science China(Life Sciences) 2011年第11期54卷 981-987页

作者： REN ZhenHua WANG JiaYin ZOU ChunLin GUAN YunQian ZHANG Yu Alex cell therapy Center Xuanwu Hospital Capital Medical University and Key Laboratory of Neurodegeneration Ministry of Education Beijing 100053 China Department of Anatomy Anhui Medical University Hefei 230032 China

Recently,transplantation of allogeneic and autologous cells has been used for regenerative medicine.A critical issue is monitoring migration and homing of transplanted cells,as well as engraftment efficiency and functional capability in *** of superparamagnetic iron oxide(SPIO) particles by magnetic resonance imaging(MRI) has been used in animal models and clinical settings to track labeled cells.A major limitation of MRI is that the signals do not show biological characteristics of transplanted cells in *** marrow mesenchymal stem cells(MSCs) have been extensively investigated for their various therapeutic properties,and exhibit the potential to differentiate into cells of diverse *** this study,cynomolgus monkey MSCs(cMSCs) were labeled with Molday ION Rhodamine-BTM(MIRB),a new SPIO agent,to investigate and characterize the biophysical and MRI properties of labeled cMSCs in vitro and in *** results indicate that MIRB is biocompatible and useful for cMSCs labeling and cell tracking by multimodality *** method is helpful for detection of transplanted stem cells in vivo,which is required for understanding mechanisms of cell therapy.

关键词： bone marrow mesenchymal stem cells MRI Molday ION Rhodamine-BTM transplantation nonhuman primate

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Semi-3D cultures using Laminin 221 as a coating material for human induced pluripotent stem cells

引用

Regenerative Biomaterials 2022年第1期9卷 775-793页

作者： Yoshiki Nakashima Shinsuke Yoshida Masayoshi Tsukahara Kyoto University Center for iPS cell Research and Application Foundation(CiRA Foundation) Facility for iPS Cell Therapy(FiT)Kyoto 606-8397Japan

It was previously believed that human induced pluripotent stem cells(hiPSCs)did not show adhesion to the coating material Laminin 221,which is known to have specific affinity for *** this study,we report that human mononuclear cell-derived hiPSCs,established with Sendai virus vector,form peninsular-like colonies rather than embryonic stem cell-like colonies;these peninsular-like colonies can be passaged more than 10 times after ***,initializationdeficient cells with residual Sendai virus vector adhered to the coating material Laminin 511 but not to Laminin ***,the expression of undifferentiated markers tended to be higher in hiPSCs established on Laminin 221 than on Laminin *** Laminin 221,hiPSCs15M66 showed a semi-floating colony *** expression of various markers of cell polarity was significantly lower in hiPSCs cultured on Laminin 221 than in hiPSCs cultured on Laminin ***,201B7 and 15M66 hiPSCs showed 3D cardiomyocyte differentiation on Laminin ***,the coating material Laminin 221 provides semi-floating culture conditions for the establishment,culture and induced differentiation of hiPSCs.

关键词： human induced pluripotent stem cells(hiPSCs) regenerative medicine coating material cardiomyocyte therapeutic cells

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Treating amyotrophic lateral sclerosis with allogeneic Schwann cell-derived exosomal vesicles: a case report

引用

Neural Regeneration Research 2025年第4期20卷 1207-1216页

作者： Pascal J.Goldschmidt-Clermont Aisha Khan George Jimsheleishvili Patricia Graham Adriana Brooks Risset Silvera Alexander J.P.Goldschmidt Damien D.Pearse W.Dalton Dietrich Allan D.Levi James D.Guest Miller School of Medicine University of MiamiMiamiFLUSA Stem cell Institute Miller School of MedicineUniversity of MiamiMiamiFLUSA The Miami Project to Cure Paralysis Miller School of MedicineUniversity of MiamiMiamiFLUSA Alzady International LLC MiamiFLUSA Neurological Surgery Miller School of MedicineUniversity of MiamiMiamiFLUSA

Schwann cells are essential for the maintenance and function of motor neurons,axonal networks,and the neuromuscular *** amyotrophic lateral sclerosis,where motor neuron function is progressively lost,Schwann cell function may also be ***,important signaling and potential trophic activities of Schwann cell-derived exosomal vesicles have been *** case report describes the treatment of a patient with advanced amyotrophic lateral sclerosis using serial intravenous infusions of allogeneic Schwann cell-derived exosomal vesicles,marking,to our knowledge,the first instance of such *** 81-year-old male patient presented with a 1.5-year history of rapidly progressive amyotrophic lateral *** initial diagnosis,the patient underwent a combination of generic riluzole,sodium phenylbutyrate for the treatment of amyotrophic lateral sclerosis,and *** patient volunteered to participate in an FDA-approved single-patient expanded access treatment and received weekly intravenous infusions of allogeneic Schwann cell-derived exosomal vesicles to potentially restore impaired Schwann cell and motor neuron *** confirmed that cultured Schwann cells obtained from the amyotrophic lateral sclerosis patient via sural nerve biopsy appeared impaired(senescent)and that exposure of the patient’s Schwann cells to allogeneic Schwann cell-derived exosomal vesicles,cultured expanded from a cadaver donor improved their growth capacity in *** a period of observation lasting 10 weeks,during which amyotrophic lateral sclerosis Functional Rating Scale-Revised and pulmonary function were regularly monitored,the patient received weekly consecutive infusions of 1.54×1012(×2),and then consecutive infusions of 7.5×1012(×6)allogeneic Schwann cell-derived exosomal vesicles diluted in 40 mL of Dulbecco’s phosphate-buffered *** of the infusions were associated with adverse events such as infusion reactions(allergic or otherwise)or

关键词： allogeneic amyotrophic lateral sclerosis exosomes infusion neuromuscular junction Schwann cell

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Implanting 1.1B4 human β-cell pseudoislets improves glycaemic control in diabetic severe combined immune deficient mice

引用

World Journal of diabetes 2016年第19期7卷 523-533页

作者： Alastair D Green Srividya Vasu Neville H McClenaghan Peter R Flatt SAAD Centre for Pharmacy and diabetes University of Ulster cell Growth and Metabolism Section National Institute for Diabetes and Digestive and Kidney diseasesNational Institutes of Health

AIM To investigate the potential of implanting pseudoislets formed from human insulin-releasing β-cell lines as an alternative to islet transplantation. METHODS In this study, the anti-diabetic potential of novel human insulin releasing 1.1B4 β-cells was evaluated by implanting the cells, either as free cell suspensions, or as three-dimensional pseudoislets, into the subscapular region of severe combined immune deficient mice rendered diabetic by single high-dose administration of streptozotocin. Metabolic parameters including food and fluid intake, bodyweight and blood glucose were monitored throughout the study. At the end of the study animals were given an intraperitoneal glucosetolerance test. Animals were then culled and blood and tissues were collected for analysis. Insulin and glucagon contents of plasma and tissues were measured by insulin radioimmunoassay and chemiluminescent enzyme-linked immunosorbance assay respectively. Histological analyses of pancreatic islets were carried out by quantitative fluorescence immunohistochemistry staining. RESULTS Both pseudoislet and cell suspension implants yielded well vascularised β-cell masses of similar insulin content. This was associated with progressive amelioration of hyperphagia(P cell(P cell(P cell suspensions eventually exhibited hypoglycaemic complications, pseudoislet recipients displayed a more gradual amelioration of diabetes, and achieved stable blood glucose control similar to non-diabetic mice at the end of the study. CONCLUSION Although further work is needed to address safety issues, these results provide proof of concept for possible therapeutic applica

关键词： Human β-cell line 1.1B4 cell therapy Insulin Pseudoislets

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Determination of glucose deficiency-induced cell death by mitochondrial ATP generation-driven proton homeostasis

引用

Journal of Molecular cell Biology 2017年第5期9卷 395-408页

作者： Yanfen Cui Yuanyuan Wang Miao Liu Li Qiu Pan Xing Xin Wang Guoguang Ying Binghui Li Laboratory of Cancer cell Biology Key Laboratory of Breast Cancer Prevention and Therapy National Clinical Research Center for Cancer Tianjin MedicalUniversity Cancer Institute and Hospital Tianjin 300060 China The First Department of Breast Tumor Key Laboratory of Breast Cancer Prevention and Therapy National Clinical Research Center for Cancer Tianjin MedicalUniversity Cancer Institute and Hospital Tianjin 300060 China

Glucose is one of major nutrients and its catabolism provides energy and/or building bricks for cell proliferation. Glucose deficiency results in cell death. However, the underlying mechanism still remains elusive. By using our recently developed method to monitor real-time cellular apoptosis and necrosis, we show that glucose deprivation can directly elicit necrosis, which is promoted by mito- chondrial impairment, depending on mitochondrial adenosine triphosphate （ATP） generation instead of ATP depletion. We demon- strate that glucose metabolism is the major source to produce protons. Glucose deficiency leads to lack of proton provision while mitochondda[ electron transfer chain continues consuming protons to generate energy, which provokes a compensatory lysosomal proton efflux and resultant increased lysosomal pH. This [ysosomal aikaUnization can trigger apoptosis or necrosis depending on the extent of alkalinization. Taken together, our results build up a metabolic connection between glycolysis, mitochondrion, and iysosome, and reveal an essential role of glucose metabolism in maintaining proton homeostasis to support cell survival.

关键词： glucose deficiency apoptosis necrosis proton homeostasis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：