检索结果-南通市图书馆

cNPAS2 inducedβcell dysfunction by regulating KANK1 expression in type 2 diabetes

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Diabetes 2024年第9期15卷 1932-1941页

作者： Yan-Bin Yin Wei Ji Ying-Lan Liu Qian-Hao Gao Dong-Dong He Shi-Lin Xu Jing-Xin Fan Li-Hai Zhang Department of General Surgery The First Affiliated Hospital of Jiamusi UniversityJiamusi 154000Heilongjiang ProvinceChina Department of Anesthesiology Yantai Affiliated Hospital of Binzhou Medical UniversityYantai 264199Shandong ProvinceChina Operating room The First Affiliated Hospital of Jiamusi UniversityJiamusi 154000Heilongjiang ProvinceChina Department of Anesthesiology Huazhong University of Science and Technology Union Jiangbei HospitalWuhan 430100Hubei ProvinceChina Department of Endocrinology The First Affiliated Hospital of Jiamusi UniversityJiamusi 154000Heilongjiang ProvinceChina

BACKGROUND Diabetes mellitus type 2(T2DM)is formed by defective insulin secretion with the addition of peripheral tissue resistance of insulin *** has been affecting over 400 million people all over the *** To explore the pathogenesis of T2DM and to develop and implement new prevention and treatment strategies for *** Receiver operating characteristic(ROC)curve analysis was used to conduct diagnostic *** expression level of genes was determined by reverse transcription-PCR as well as Western *** proliferation assays were performed by cell counting kit-8(CCK-8)*** last,T2DM mice underwent Roux-en-Y gastric bypass *** We found that NPAS2 was significantly up-regulated in isletβcell apoptosis of *** ROC curve revealed that NPAS2 was capable of accurately diagnosing ***2 overexpression did increase the level of *** addition,the CCK-8 test revealed knocking down NPAS2 and KANK1 increased the proliferation of MIN6 *** last,we found that gastric bypass may treat type 2 diabetes by down-regulating NPAS2 and *** This study demonstrated that NPAS2 inducedβcell dysfunction by regulating KANK1 expression in type 2 diabetes,and it may be an underlying therapy target of T2DM.

关键词： Diabetes mellitus type 2 KANK1 NPAS2 Gastric bypass

Migration of Resovist-labeled neural stem cells towards focal rat cerebral ischemic regions as determined by in vivo tracking and magnetic resonance imaging

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2010年第13期5卷 970-974页

作者： Shuang Wang Yuguang Zhao Xiaobo Yan Kuang Fu Lihua Wang Department of Neurology Second Affiliated Hospital Harbin Medical University Harbin 150086 Heilongjiang Province China Department of cell biology Harbin Medical University Harbin 150081 Heilongjiang Province China room of Nuclear Magnetic Resonance Second Affiliated Hospital Harbin Medical University Harbin 150086 Heilongjiang Province China

BACKGROUND： Resovist, a superparamagnetic iron oxide, can be used to label neural stem cells （NSCs）. Magnetic resonance tracking of superparamagnetic iron oxide-labeled NSCs is a non-invasive technique to track transplanted NSCs following focal cerebral ischemia. OBJECTIVE： To observe survival and migration of transplanted NSCs in a rat model of focal ischemia/repeffusion using magnetic resonance imaging （MRI）. DESIGN, TIME AND SETTING： An in vitro, in vivo, tracking study was performed at the Basic Laboratory of Harbin Medical University and the room of MRI, Second Affiliated Hospital of Harbin Medical University, China from December 2006 to December 2009. MATERIALS： Resovist （Schering, Germany） and Achieva 1.5TMR imaging system （Philips, Amsterdam, the Netherlands） were utilized in the present study. METHODS： NSCs were harvested from brain tissues of neonatal Sprague Dawley rats and were labeled with Resovist （11.2μg/mL and 5 ×10^5 cells/mL）. A total of 15 adult, Sprague Dawley rats were randomly assigned to model （n = 9） and control （n = 6） groups. All rats were utilized to establish models of middle cerebral artery occlusion. Rats in the model group were subjected to Resovist-labeled NSCs transplantation by injection of cell suspension into both ventricles （5μL/ ventricle）. Rats in the control group were treated with an equal volume of physiological saline. MAIN OUTCOME MEASURES： Immunocytochemistry, transmission electron microscopy, and Prussian blue staining were employed to observe whether cells phagocytized iron particles. In addition, 3-（4, 5-dimethylthiazol-2-yl）-2, 5-diphenyltetrazolium bromide assay was used to measure viability and differentiation of NSCs labeled by various concentrations of Resovist. MRI was used to trace survival and migration of Resovist-labeled NSCs. RESULTS： Following Resovist and NSCs co-incubation, Prussian blue staining revealed iron particles in cells. In addition, staining was observed in daughter cells following cell division

关键词： neural stem cells Resovist magnetic resonance imaging superparamagnetic iron oxide tracking neural regeneration

维普期刊数据库

Actin Fringe Is Correlated with Tip Growth Velocity of Pollen Tubes

在线全文

学校读者我要写书评

暂无评论

Molecular Plant 2012年第5期5卷 1160-1162页

作者： Huaijian Dong Weike Pei Ren Haiyun Key Laboratory of cell Proliferation and Regulation biology of Ministry of Education and College of Life Science Beijing Normal University Beijing 100875 China

Dear Editor, As an essential element for pollen tube tip growth, actJn cytoskeleton has been a focus for decades. Recently, both rapid-freeze and room-temperature fixation have revealed the presence of a subapical cortical F-actin （actin fringe） as a consistent feature of lily pollen tubes （Lovy-Wheeler et al., 2005）. The actin fringe consists of a palisade of short longitudinally oriented actin cables that start about 1-5~m behind the extreme apex and extend basally for an additional 5-10pm （Lovy-Wheeler et al., 2005）. MeanwhJJe, observations in live cells using Lifeact-mEGFP, an actin probe consisting of the first 17 amino acids from the budding yeast ABP-140 fused to GFP, which does not affect the dynamics of actin, compellingly support the presence of a cortical actin fringe in lilv pollen tubes ~Vidali et al.. 2009~.

关键词： F-肌动蛋白花粉管生长速度氨基酸组成必需元素顶端生长快速冷冻芽殖酵母

Effect of propofol on generation of inflammatory mediator of monocytes

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Pacific Journal of Tropical Medicine 2015年第11期8卷 940-945页

作者： Yun Nie Yan-Xi Lu Li-Hong Lv Operating room First People's Hospital of Ji'nan

Objective:To evaluate the effect of propofol with different concentrations on the expression of inflammatory mediators of mterleukin and tumor-necrosis factor- α(TNF- α) by stimulating the mouse primary monocytes and human monocytic cell line with lipopolysaccharide(LPS)and also discuss the effeet of propofol on the secretion of inflammatory mediator and its possible molecular ***:The mononuclear cells of mouse spleen were separated and then purified to obtain the primary *** dose-effect relationship of production of pro-inflammatory cytokines by monocytes which were stimulated by LPS,namely the monocytes were stimulated by the dose of 0-500 ng/mL for 24 *** was employed to detect the concentration of IL-6,TL-8 and TNF-α.The effect of propofol on the secretion of above pro-inflammatory cytokines by the monocytes was *** were divided into the control group,the 0.1%DMSO group,the LPS group and the treatment group with LPS + different dose of propofol(propofol 1-100 μg/mL).ELISA was employed to detect the concentration of IL-6,IL-8 and TNF-α.The change in the expression of important signaling molecules in Toll-like receptor and NF-κ B signaling pathway was detected after THP-1 cells were treated with ***:The concentration of TNF- α was(3 863±153)pg/mL after 12 h of stimulation by LPS and then its concentration was decreased *** the concentration of IL-6 and IL-8 was relatively high after 24 h of stimulation by LPS,(5 627±330) pg/mL and(1 626±200) pg/mL,*** propofol could inhibit the expression of IL-6,IL-8 and TNF- α caused by *** the intervention treatment of 50 μg/mL propofol,the concentration of IL-6,IL-8 and TNF-α was significantly decreased(P<0.01).Conclusions:The propofol can inhibit the expression of TLR-4 and NF-κ B to inhibit the activation of p38 and the expression of pro-inflammatory cytokines.

关键词： Propofol LPS IL-6 IL-8 TNF-α Anti-inflammation

Studies on the Temperature Effect on Bacteriorhodopsin of Purple and Blue Membrane by Fluorescence and Absorption Spectroscopy

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Acta Biochimica et Biophysica Sinica 2006年第10期38卷 691-696页

作者： Lan-Ying CHENG~(1,2) Yue ZHANG~3 Shi-Gui LIU~1 Kun-Sheng HU~3 Kang-Cheng RUAN~2* 1 College of Life Science,Sichuan University,Chengdu 610064,China 2 State Key Laboratory of Molecular biology,Institute of Biochemistry and cell biology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200031,China 3 Institute of Biophysics,Academia Sinica,Beijing 100101,China State Key Laboratory of Molecular biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China

Fluorescence and absorption spectra were used to study the temperature effect on theconformation of bacteriorhodopsin (bR) in the blue and purple membranes (termed as bRb and bRprespectively).The maximum emission wavelengths of tryptophan fluorescence in both proteins at roomtemperature are 340 nm,and the fluorescence quantum yield of bRb is about 1.4 fold higher than that of *** temperature increases,the tryptophan fluorescence of bRb decreases,while the tryptophan fluorescenceof bRp *** binding study of extrinsic fluorescent probe bis-ANS indicated that the probe can bindonly to bRb,but not to *** results suggest that significant structural difference existed between bRband *** was also found that both kinds of bR are highly thermal *** maximum wavelength of theprotein fluorescence emission only shifted from 340 nm to 346 nm at 100℃.More interestingly,as tempera-ture increased,the characteristic absorption peak of bRb at 605 nm decreased and a new absorption peak at380 nm *** transition occurred at a narrow temperature range (65℃-70℃).These facts indicatedthat an intermediate can be induced by high *** phenomenon has not been reported before.

关键词： bacteriorhodopsin purple membrane blue membrane fluorescence and absorption spectroscopy

Fatty Acid Composition in Freeze-Dried Chinese Mitten Crabs (<i>Eriocheir sinensis</i>)

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Open Journal of Marine Science 2012年第3期2卷 90-95页

作者： Lihong He Feng Li Junyong Tang Department of cell biology Medical College Soochow University Suzhou China

Freeze drying is reported to be the best method of dehydration. Live fresh Chinese mitten crabs (Eriocheir sinensis) were freeze dried. The moisture content, rehydration ratio, and fatty acid composition of freeze-dried crabs were analysed. The applicability of using freeze drying to process high-value E. sinensis, so as to prolong the time duration of their storage and marketing, were discussed. After lyophilisation, the average moisture content was 6%. The physical properties (shape, size, and colour) of the musculature and viscera were maintained well during freeze drying. The rehydration ratio was 2.15 when rehydrated for 30 min at room temperature. The levels of polyunsaturated fatty acids, especially eicosapentaenoic acid and docosahexaenoic acid, were higher in female freeze-dried crabs than in male crabs. After full rehydration, the fatty acid composition of freeze-dried crabs showed no significant differences to that of frozen crabs after thawing. In conclusion, freeze drying can well preserve the physical properties of the edible parts and fatty acid composition of the viscera in high-value E. sinensis. Rehydration has no destruction of the nutritional value regarding to the fatty acid composition. Therefore, freeze drying is a suitable technique for the processing of high-value E. sinensis.

关键词： Eriocheir sinensis Fatty Acid Composition Freeze Drying Frozen Storage Rehydration Ratio Viscera

Observation of a single protein by ultrafast X-ray diffraction

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Light(Science & Applications) 2024年第1期13卷 80-90页

作者： Tomas Ekeberg Dameli Assalauova Johan Bielecki Rebecca Boll Benedikt J.Daurer Lutz A.Eichacker Linda E.Franken Davide E.Galli Luca Gelisio Lars Gumprecht Laura H.Gunn Janos Hajdu Robert Hartmann Dirk Hasse Alexandr Ignatenko Jayanath Koliyadu Olena Kulyk Ruslan Kurta Markus Kuster Wolfgang Lugmayr Jannik Lübke Adrian P.Mancuso Tommaso Mazza Carl Nettelblad Yevheniy Ovcharenko Daniel E.Rivas Max Rose Amit K.Samanta Philipp Schmidt Egor Sobolev Nicusor Timneanu Sergey Usenko Daniel Westphal Tamme Wollweber Lena Worbs Paul Lourdu Xavier Hazem Yousef Kartik Ayyer Henry N.Chapman Jonas A.Sellberg Carolin Seuring Ivan A.Vartanyants Jochen Küpper Michael Meyer Filipe R.N.C.Maia Laboratory of Molecular Biophysics Department of Cell and Molecular BiologyUppsala UniversityHusargatan 3(Box 596)SE-75124 UppsalaSweden Deutsches Electronen-Synchrotron DESY Notkestrasse 8522607 HamburgGermany European XFEL Holzkoppel 422869 SchenefeldGermany Diamond Light Source Harwell Science&Innovation CampusDidcot OX110DEUK University of Stavanger Centre Organelle ResearchRichard-Johnsensgate 44021 StavangerNorway Leibniz Institute for Experimental Virology(HPI) Centre for Structural Systems BiologyNotkestraße 8522607 HamburgGermany Dipartimento di Fisica“Aldo Pontremoli” Universitàdegli Studi di Milanovia Celoria 1620133 MilanoItaly Center for Free-Electron Laser Science DESY22607 HamburgGermany Plant biology Section School of Integrative Plant ScienceCornell UniversityIthacaNY 14853USA PNSensor GmbH Otto-Hahn-Ring 6D-81739 MünchenGermany Biomedical and X-Ray Physics Department of Applied PhysicsAlbaNova University CenterKTH Royal Institute of TechnologySE-10691 StockholmSweden ELI Beamlines/IoP Institute of Physics AS CR v.v.i.Na Slovance 218221 Prague 8Czech Republic Multi-User CryoEM Facility Centre for Structural Systems BiologyNotkestr.8522607 HamburgGermany University Medical Center Hamburg-Eppendorf(UKE) Martinistrasse 5220246 HamburgGermany The Hamburg Center for Ultrafast Imaging Universität HamburgLuruper Chaussee 14922761 HamburgGermany Department of Physics Universität HamburgLuruper Chaussee 14922761 HamburgGermany Department of Chemistry and Physics La Trobe Institute for Molecular ScienceLa Trobe UniversityMelbourneVIC 3086Australia Division of Scientific Computing Science for Life LaboratoryDepartment of Information TechnologyUppsala UniversityBox 337SE-75105 UppsalaSweden European Molecular biology Laboratory c/o DESYNotkestrasse 8522607 HamburgGermany Department of Physics and Astronomy Uppsala UniversityBox 516SE-75120 UppsalaSweden Max Planck Institute for the Structure and Dynamics of Matter Luruper Chaussee 14922761 HamburgGe

The idea of using ultrashort X-ray pulses to obtain images of single proteins frozen in time has fascinated and inspired *** was one of the arguments for building X-ray free-electron *** to theory,the extremely intense pulses provide sufficient signal to dispense with using crystals as an amplifier,and the ultrashort pulse duration permits capturing the diffraction data before the sample inevitably *** was first demonstrated on biological samples a decade ago on the giant *** then,a large collaboration has been pushing the limit of the smallest sample that can be *** ability to capture snapshots on the timescale of atomic vibrations,while keeping the sample at room temperature,may allow probing the entire conformational phase space of *** we show the first observation of an X-ray diffraction pattern from a single protein,that of Escherichia coli GroEL which at 14 nm in diameter is the smallest biological sample ever imaged by X-rays,and demonstrate that the concept of diffraction before destruction extends to single *** the pattern,it is possible to determine the approximate orientation of the *** experiment demonstrates the feasibility of ultrafast imaging of single proteins,opening the way to single-molecule time-resolved studies on the femtosecond timescale.

关键词： smallest intense ultrafast

SIRT5 is important for bacterial infection by regulating insulin secretion and glucose homeostasis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Protein & cell 2020年第11期11卷 846-851页

作者： Cuiping Zhang Ke Wang Zuojian Hu Lujie Yang Bin Wei Shan Li Xue Qin Pengyuan Yang Hongxiu Yu Minhang Hospital Institutes of Blomedical Scliences Department of Systems Biology for MedicineSchool of Basic Medical SciencesFudan UniversityShanghai 200032China Department of Clinical Laboratory First Afliated Hospital of Guangxi Medical UniversityNanning 530021China School of Life Science Shanghai UniversityShanghai 200444China Minhang Hospital Institutes of Biomedical Sciences Department of Systems Biology for MedicineSchool of Basic Medical SciencesFudan UniversityRoom 501Mingdao Building131 Dongan RoadShanghai 200032China Minhang Hospital Institutes of Biomedical Sciences Department of Systems Biology for MedicineSchool of Basic Medical SciencesFudan UniversityRoom 201Mingdao Building131 Dongan RoadShanghai 200032China

Dear Editor,Recent studies indicate that cellular metabolism plays a key role in supporting immune cell development,maintenance and function(Norata et al.,2015).For example,sensing of microbial ligands by macrophages triggers increased glucose metabolism,which delivers energy to support antimicrobial inflammation and the production of *** effect further enhances the generation of mitochondrial reactive oxygen species(ROS)to fight infections.

关键词： metabolism supporting homeostasis

维普期刊数据库

Chemokine receptor 4 gene silencing blocks neuroblastoma metastasis in vitro

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2014年第10期9卷 1063-1067页

作者： Xin Chen Yongjie Zhu Lulu Han Hongting Lu Xiwei Hao Qian Dong Department of Pediatric Surgery the Affiliated Hospital of Qingdao University Operating room the Affiliated Hospital of Qingdao University

This study investigated the effects of small interfering RNA （siRNA）-mediated silencing of chemokine receptor 4 （CXCR4） on the invasion capacity of human neuroblastoma cell line SH-SY5Y in vitro. Three siRNAs targeting CXCR4 were chemically synthesized and individually transfected into SH-SY5Y cells. Expression of CXCR4 mRNA and protein was signiifcantly sup-pressed in transfected cells by all three sequence-speciifc siRNAs compared with control groups. Furthermore, the invasion capacity of SH-SY5Y cells was signiifcantly decreased following trans-fection with CXCR4-speciifc siRNA compared with the control groups. These data demonstrate that down-regulation of CXCR4 can inhibit in vitro invasion of neuroblastoma.

关键词： nerve regeneration chemokine receptor 4 small interfering RNA neuroblastoma inva-sion Transwell chamber liposome NSFC grant neural regeneration

维普期刊数据库