Chemokine receptor 4 gene silencing blocks neuroblastoma metastasis in vitro

作     者：Xin Chen Yongjie Zhu Lulu Han Hongting Lu Xiwei Hao Qian Dong 

作者机构：Department of Pediatric Surgerythe Affiliated Hospital of Qingdao University Operating Roomthe Affiliated Hospital of Qingdao University 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2014年第9卷第10期

页      面：1063-1067页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100214[医学-肿瘤学] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China,No.81272986 the Natural Science Foundation of Shandong Province,No.ZR2011HZ002 

主　　题：nerve regeneration chemokine receptor 4 small interfering RNA neuroblastoma inva-sion Transwell chamber liposome NSFC grant neural regeneration 

摘      要：This study investigated the effects of small interfering RNA （siRNA）-mediated silencing of chemokine receptor 4 （CXCR4） on the invasion capacity of human neuroblastoma cell line SH-SY5Y in vitro. Three siRNAs targeting CXCR4 were chemically synthesized and individually transfected into SH-SY5Y cells. Expression of CXCR4 mRNA and protein was signiifcantly sup-pressed in transfected cells by all three sequence-speciifc siRNAs compared with control groups. Furthermore, the invasion capacity of SH-SY5Y cells was signiifcantly decreased following trans-fection with CXCR4-speciifc siRNA compared with the control groups. These data demonstrate that down-regulation of CXCR4 can inhibit in vitro invasion of neuroblastoma.