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Chemokine receptor 4 gene silencing blocks neuroblastoma metastasis in vitro

Neural Regeneration Research 2014年第10期9卷 1063-1067页

作者： Xin Chen Yongjie Zhu Lulu Han Hongting Lu Xiwei Hao Qian Dong Department of Pediatric Surgery the Affiliated Hospital of Qingdao University Operating Room the Affiliated Hospital of Qingdao University

This study investigated the effects of small interfering RNA （siRNA）-mediated silencing of chemokine receptor 4 （CXCR4） on the invasion capacity of human neuroblastoma cell line SH-SY5Y in vitro. Three siRNAs targeting CXCR4 were chemically synthesized and individually transfected into SH-SY5Y cells. Expression of CXCR4 mRNA and protein was signiifcantly sup-pressed in transfected cells by all three sequence-speciifc siRNAs compared with control groups. Furthermore, the invasion capacity of SH-SY5Y cells was signiifcantly decreased following trans-fection with CXCR4-speciifc siRNA compared with the control groups. These data demonstrate that down-regulation of CXCR4 can inhibit in vitro invasion of neuroblastoma.

关键词： nerve regeneration chemokine receptor 4 small interfering RNA neuroblastoma inva-sion Transwell chamber liposome NSFC grant neural regeneration

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Effects of hypoxia inducible factor-1alpha siRNA on theinvasion of human Hela cells and expression of relatedproteins

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Frontiers of Medicine 2009年第3期3卷 303-308页

作者： Bin YANG Xianglin YUAN Yanmei ZOU Qingsong XI Guoxian LONG Qiang FU Guangyuan HU Center of Oncology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China First Ward of Internal Medicine Tumor Hospital of Hubei ProvinceWuhan 430067China

The effects of hypoxia on the invasion and the related protein expression of Hela cells and the role of hypoxia inducible factor-1α(HIF-1α)were *** Hela cells were divided into three groups,namely,H0(non-transfected Hela cells),H1(pGenesil-1 empty plasmid-transfected Hela cells),and H2(HIF-1α-shRNA plasmid-transfected Hela cells),and were cultured under hypoxia(1%O2)and normoxia for 48 *** expression of HIF-1α,E-cadherin,β-catenin,and actin was detected using Western *** scratch test and the invasion assay were applied to examine the invasion in each *** expression of HIF-1α,E-cadherin,andβ-catenin in tumor grafts was assayed *** blot results revealed that the bands of HIF-1α,E-cadherin,β-catenin,and actin proteins were detected in the H0 and H1 groups under hypoxia for 48 *** expression of E-cadherin,β-catenin,and actin was detected in the H2 group under hypoxia for 48 h,and *** the H0,H1,and H2 groups under normoxia,and the H2 group under hypoxia for 48 h,no expression of HIF-1αwas *** scratch test showed that the invasive ability in the H2 group was signiﬁcantly ***-cally,it was found that there was a signiﬁcant difference in the expression of HIF-1α,E-cadherin,andβ-catenin between the H1 and H2 groups(Pinvasion of human cervical cancer Hela cells and the expression of related proteins(E-cadherin,β-catenin,and actin)depend on HIF-1α.

关键词： hypoxia actin β-catenin E-cadherin inva-sion

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