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Robust transcriptional indicators of immune cell death revealed by spatiotemporal transcriptome analyses

Molecular Plant 2022年第6期15卷 1059-1075页

作者： Jose Salguero-Linares Irene Serrano Nerea Ruiz-Solani Marta Salas-Gómez Ujjal Jyoti Phukan Victor Manuel González MartíBernardo-Faura Marc Valls David Rengel Nuria S.coll Centre for Research in Agricultural Genomics(CRAG) CSIC-IRTA-UAB-UBCampus UABBellaterra08193 BarcelonaSpain LIPM Universitéde ToulouseINRACNRS84195 Castanet-TolosanFrance INRAE GeT-PlaGeGenotoul31326 Castanet-TolosanFrance Department of Genetics Universitat de Barcelona08028 BarcelonaSpain Consejo Superior de Investigaciones Científicas(CSIC) BarcelonaSpain Department of Plant Molecular Biology and Physiology.Albrechtvon Haller Institute for Plant Sciences niversity of GottingenJulia-Lermontowa-Weg3.37077 GottingenGrmany Institut de Phammacologie et de Biologie Structurale IPBS Universite deToulouse CNRSUPSBP 64182205 route de Nartxonne310077 Toulouse Cedex 04France

Recognition of a pathogen by the plant immune system often triggers a form of regulated cell death traditionally known as the hypersensitive response(HR).This type of cell death occurs precisely at the site of pathogen recognition,and it is restricted to a few *** research has shed light on how plant immune receptors are mechanistically ***,two central key questions remain largely unresolved:how does cell death zonation take place,and what are the mechanisms that underpin this phenomenon?Consequently,bona fide transcriptional indicators of HR are lacking,which prevents deeper insight into its mechanisms before cell death becomes macroscopic and precludes early or live *** this study,to identify the transcriptional indicators of HR we used the paradigmatic Arabidopsis thaliana–Pseudomonas syringae pathosystem and performed a spatiotemporally resolved gene expression analysis that compared infected cells that will undergo HR upon pathogen recognition with bystander cells that will stay alive and activate *** data revealed unique and time-dependent differences in the repertoire of differentially expressed genes,expression profiles,and biological processes derived from tissue undergoing HR and that of its ***,we generated a pipeline based on concatenated pairwise comparisons between time,zone,and treatment that enabled us to define 13 robust transcriptional HR *** these genes,the promoter of an uncharacterized AAA-ATPase was used to obtain a fluorescent reporter transgenic line that displays a strong spatiotemporally resolved signal specifically in cells that will later undergo pathogen-triggered cell *** valuable set of genes can be used to define cells that are destined to die upon infection with HR-triggering bacteria,opening new avenues for specific and/or high-throughput techniques to study HR processes at a single-cell level.

关键词： Arabidopsis thaliana cell death indicator effector-triggered immunity hypersensitive response pattern-triggered immunity plant immunity Pseudomonas syringae

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Spatio-temporal visualization of light transport in complex photonic structures

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Light(Science & Applications) 2016年第1期5卷 714-722页

作者： Lorenzo Pattelli Romolo Savo Matteo Burresi Diederik S Wiersma European Laboratory for Non-linear Spectroscopy(LENS) Universitàdi FirenzeSesto Fiorentino(FI)50019Italy Istituto Nazionale di Ottica(CNR-INO) Firenze(FI)50125Italy Dipartimento di Fisica e Astronomia Universitàdi FirenzeSesto Fiorentino(FI)50019Italy Laboratoire Kastler Brossel UMR 8552CNRSEcole Normale SupérieureUniversitéPierre et Marie CurieCollège de France24 rue LhomondParis 75005France Gestione SILO Srl via di Castelpulci 14/dScandicci(FI)50010Italy

Spatio-temporal imaging of light propagation is very important in photonics because it provides the most direct tool available to study the interaction between light and its host ***-ps time resolution is needed to investigate the fine and complex structural features that characterize disordered and heterogeneous structures,which are responsible for a rich array of transport physics that have not yet been fully explored.A newly developed wide-field imaging system enables us to present a spatiotemporal study on light transport in various disordered media,revealing properties that could not be properly assessed using standard *** extending our investigation to an almost transparent membrane,a configuration that has been difficult to characterize until now,we unveil the peculiar physics exhibited by such thin scattering systems with transport features that go beyond mainstream diffusion modeling,despite the occurrence of multiple scattering.

关键词： diffusive approximation disordered media light transport optical gating wide-field imaging

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Synthesis of New 2-Phenylamino-4<i>H</i>-chromene-3-carbonitrile Derivatives and Their Effects on Tumor Cell Lines and against Protein Kinases

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International Journal of Organic Chemistry 2020年第2期10卷 88-103页

作者： Ali Bouattour Mehdi Fakhfakh Souhir Abid Ludovic Paquin Rémy Le Guével Thierry Charlier Sandrine Ruchaud Stéphane Bach Jean-Pierre Bazureau Houcine Ammar Laboratoire de Chimie Appliquée: Hétérocycles Corps Gras et Polymères Faculté des Sciences de Sfax Université de Sfax Sfax Tunisie Département de Chimie Collège des Sciences et des Arts Université de Jouf Al Qurayyat KSA Institut des Sciences Chimiques de Rennes (ISCR) UMR CNRS 6226 Université de Rennes 1 Campus de Beaulieu Rennes France S2 Wave Platform ScanMAT UMS 2001CNRS Université de Rennes 1 Campus de Beaulieu Rennes France Université de Rennes 1 ImPACcell Platform Campus Villejean Rennes France Sorbonne Universités UPMC Université Paris 06 CNRS USR3151 Protein Phosphorylation and Human Disease Unit KISSf Platform Station Biologique Place Georges Teissier Roscoff France

The synthesis of 2-phenylimino-4H-chromene-3-carbonitriles 6(a-d) in good overall yields using an efficient and practical methodology in 3 steps has been implemented in this present work. The first step was a heterocyclization between 2-hydroxybenzaldehyde 1 and propanedinitrile 2 which produced 2-iminocoumarin 3 which was submitted to nitrogen/nitrogen displacement in the presence of aromatic primary amine 4. In the third step, reduction of 5 led to the desired 2-phenylimino-4H-chromene-3-carbonitriles 6. Compounds 5(a-d) and 6(a-d) were evaluated for their potential in vitro cytotoxicity against six selected tumor cell lines (Huh7-D12, Caco2, MDA-MB231, HCT 116, PC3 and NCI-H727) and tested for their protein kinase inhibition on eight selected protein kinases. Among them, compounds 5c and 6b exhibited inhibition on HsCK1e (5c: 44% and 6b: 42% at 1 μM) and 5c for cytotoxicity on PC3 cell lines (63% at 25 μM).

关键词： Iminocoumarin 2H-benzopyran 2-Imino-2H-benzopyran 4H-chromene 2-Amino-4H-chromene Nitrogen/Nitrogen Displacement Protein Kinase Inhibition Cytotoxicity

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顺势疗法药物白砷剂抑制暴露于三氧化二砷的大肠杆菌细胞内活性氧的产生并上调其抗三氧化二砷基因的表达(英文)

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中西医结合学报 2012年第2期10卷 210-227页

作者： Arnab De Durba Das Suman Dutta Debrup Chakraborty Naoual Boujedaini Anisur Rahman Khuda-Bukhsh Cytogenetics and Molecular Biology Laboratory Department of ZoologyUniversity of Kalyani Boiron Laboratory LyonFrance

目的:检验顺势疗法药物Arsenicum Album 30C(Ars Alb 30C)是否能够降低亚砷酸钠对大肠杆菌(Escherichiacoli)的毒性。方法:将在标准培养基中培养至对数生长期的大肠杆菌暴露于低剂量砷剂下。1或2 mmol/L亚砷酸钠单独作用于大肠杆菌作为... 详细信息

目的:检验顺势疗法药物Arsenicum Album 30C(Ars Alb 30C)是否能够降低亚砷酸钠对大肠杆菌(Escherichiacoli)的毒性。方法:将在标准培养基中培养至对数生长期的大肠杆菌暴露于低剂量砷剂下。1或2 mmol/L亚砷酸钠单独作用于大肠杆菌作为对照,在此基础上加入Ars Alb 30C作为治疗组,或加入按顺势疗法原则配置的乙醇作为安慰剂组。分别于45 min和90 min后检测大肠杆菌的葡萄糖摄取量,细胞内己糖激酶、脂质过氧化物酶、超氧化物歧化酶及过氧化氢酶活性,细胞内外亚砷酸钠含量,细胞生长情况,细胞膜电位,DNA损伤情况,细胞内活性氧、三磷酸腺苷及自由型谷胱甘肽含量,以及arsB和ptsG基因表达情况。实验按照随机分组原则及盲法原则进行。结果:暴露于亚砷酸钠的大肠杆菌的葡萄糖摄取量、细胞内活性氧、脂质过氧化反应及DNA损伤增加;己糖激酶、超氧化物歧化酶及过氧化氢酶活性降低;细胞内三磷酸腺苷及自由型谷胱甘肽含量降低;细胞膜电位降低且细胞生长缓慢;arsB和ptsG基因表达水平增高。Ars Alb 30C作用后降低了亚砷酸钠对大肠杆菌的毒性,表现为抑制细胞内活性氧的生成和对细胞生长的促进作用。结论:Ars Alb 30C能够降低亚砷酸钠对大肠杆菌的毒性,证实了这一顺势疗法原则下高度稀释的药物的效用。

关键词：砷剂大肠杆菌顺势疗法活性氧 DNA损伤腺苷三磷酸逆转录聚合酶链反应

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Context-free Grammar and Hierarchy Processing on Macaque Monkeys

Context-free Grammar and Hierarchy Processing on Macaque Mon...

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中国神经科学学会第十二届全国学术会议

作者： Xinjian Jiang Tenghai Long Stanislas Dehaene Liping Wang Institute of Neuroscience Key Laboratory of Primate Neurobiology CAS Center for Excellence in Brain Science and Intelligence Technology Chinese Academy of Sciences Key laboratory of Brain Functional Genomics Institute of Cognitive Neuroscience School of Psychology and Cognitive Science East China Normal University collège de france Cognitive Neuroimaging Unit CEA DSV/I2BM INSERM Université Paris Sud Université Paris-Saclay Neuro Spin center

What does seem to be unique to our species in both human languages and expressive power in other domains is hierarchical structure. With this fundamental characteristic, humans are endowed with a finite cognitive computational system that generates infinity of meaningful expressions. This thus has risen to an important question: To what extent does hierarchical syntax deviate from the vocal communication signals of nonhuman animals? It has been proposed that the important distinction between human and non-human animal appears to be between the levels of regular or ‘finite state’ grammars and context-free or ‘phrase structure’ grammars. However, the true grammatical ability in nonhuman animals still remained unclear. Here, we tested three macaque monkeys on a newly designed paradigm: delayed sequence recall task. On each trial the monkeys viewed two（‘A-B’） or three（‘A-B-C’） sequentially presented spatial locations in a sample period, followed by a test period that showing six locations（with three distractors） together. The task was to touch the locations by either repeating（e.g. ‘ABC|ABC’） or reversing（e.g. ‘ABC|CBA’） their original orders without touching distractors. The results showed that all three monkeys successfully learned and generated the sequence defined by a centre-embedding, context-free grammar（mirror structure） and further spontaneously generalized the rule to untrained spatial sequences. These results suggest the context-free grammar is also available to nonhuman animals and probably not uniquely human. Our paradigm should allow us test higher-level of supra-regular grammars in the Chomsky hierarchy and investigate the neural correlates of recursive rules on non-human primates.

关键词： centre-embedding context-free grammar monkey human unique recursion hierarchy

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East and West： Forging A Bridge Across Cultures--An Interview with Prof. John Blair and Prof. Jerusha McCormack

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Frontiers of Literary Studies in China-Selected Publications from Chinese Universities 2013年第1期7卷 142-153页

作者： Blair, John G. McCormack, Jerusha Liu, Yan Beijing Foreign Studies Univ Sch English & Int Studies Beijing Peoples R China Oakland Univ Rochester MI 48309 USA Univ Virginia Charlottesville VA 22903 USA Univ Strasbourg Strasbourg France Univ Geneva Dept English Ordinaire Amer Literature & Civilizat Geneva Switzerland Beijing Foreign Studies Univ Beijing Peoples R China Univ coll Dublin Dublin Ireland Boston Univ Irish Studies Program Boston MA 02215 USA Beijing Int Studies Univ Inst Transcultural Studies Beijing Peoples R China

Interviewees： Prof. John G. Blair and Prof. Jerusha McCormack （Visiting professors at Beijing Foreign Studies University, China; names abbreviated as B. and M. in the following） Interviewer＂ Prof. Yan Liu （The ...

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The role ofin colorectal cancer: from carcinogenesis to clinical management

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慢性疾病与转化医学（英文） 2019年第3期 178-187页

Colorectal cancer (CRC) is a common malignant tumor that affects people worldwide. Metagenomic analyses have shown an enrichment ofFusobacterium nucleatum (F. nucleatum) in colorectal carcinoma tissue; many studies have indicated thatF. nucleatum is closely related to the colorectal carcinogenesis. In this review, we provide the latest information to reveal the related molecular mechanisms. The known virulence factors ofF. nucleatum promote adhesion to intestinal epithelial cells via FadA and Fap2. Besides, Fap2 also binds to immune cells causing immunosuppression. Furthermore,F. nucleatum recruits tumor-infiltrating immune cells, thus yielding a pro-inflammatory microenvironment, which promotes colorectal neoplasia progression.F. nucleatum was also found to potentiate CRC development through toll-like receptor 2 (TLR2)/toll-like receptor 4 (TLR4) signaling and microRNA (miRNA)-21 expression. In addition,F. nucleatum increases CRC recurrence along with chemoresistance by mediating a molecular network of miRNA-18a*, miRNA-4802, and autophagy components. Moreover, viableF. nucleatum was detected in mouse xenografts of human primary colorectal adenocarcinomas through successive passages. These findings indicated that an increased number ofF. nucleatum in the tissues is a biomarker for the diagnosis and prognosis of CRC, and the underlying molecular mechanism can probably provide a potential intervention treatment strategy for patients withF. nucleatum-associated CRC.

关键词： Fusobacterium nucleatum Colorectal carcinoma Carcinogenesis Immune microenvironment Intervention therapy

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Inborn errors of OAS-RNase L in SARS-CoV-2-related multisystem inflammatory syndrome in children

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四川生理科学杂志 2022年第12期44卷 2223-2223页

作者： Danyel Lee Laboratory of Human Genetics of Infectious Diseases Necker Branch INSERM U1163 Paris France Paris City University Imagine Institute Paris France Laboratory of Virology and Infectious Disease The Rockefeller University New York NY USA St. Giles Laboratory of Human Genetics of Infectious Diseases Rockefeller Branch The Rockefeller University New York NY USA Department of Cancer Biology Lerner Research Institute Cleveland Clinic Cleveland OH USA Human Evolutionary Genetics Unit Institut Pasteur Paris City University CNRS UMR 2000 Paris France Doctoral college Sorbonne University Paris France Laboratory of Immunology Lyon Sud Hospital Lyon France Pediatric Intensive Care Department Hospital Sant Joan de Déu Barcelona Spain Kids Corona Platform Barcelona Spain Center for Biomedical Network Research on Epidemiology and Public Health (CIBERESP) Instituto de Salud Carlos III Madrid Spain Department of Surgery and Surgical Specializations Faculty of Medicine and Health Sciences University of Barcelona Barcelona Spain Respiratory and Immunological Dysfunction in Pediatric Critically Ill Patients Institute of Recerca Sant Joan de Déu Barcelona Spain Bursa City Hospital Bursa Turkey Ankara City Hospital Yildirim Beyazit University Ankara Turkey Immunology and Rheumatology Division Department of Pediatrics University of Montreal CHU Sainte-Justine Montreal QC Canada Human Evolutionary Genetics Unit Institut Pasteur Paris City University CNRS UMR 2000 Paris France Center for Translational Research Institut Pasteur Paris City University Paris France Laboratory of Virology and Infectious Disease The Rockefeller University New York NY USA Department of Biochemistry and Center for RNA Science and Therapeutics Case Western Reserve University Cleveland OH USA Department of Surgery Weill Cornell Medical College New York NY USA Laboratory of Virology and Infectious Disease The Rockefeller University New York NY USA Department of Pediatrics School of Me

Multisystem inflammatory syndrome in children(MIS-C)is a rare and severe condition that follows benign *** report autosomal recessive deficiencies of OAS1,OAS2,or RNASEL in five unrelated children with *** cytosolic d... 详细信息

关键词： inflammatory children multisystem

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力敏感离子通道Piezo1分布与活性的力学调控及其与细胞转化态的关系

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医用生物力学 2021年第S1期36卷 88-88页

作者：姚明曦 Ajay Tijore Charles D Cox Anushya Hariharan Guy Tran Van Nhieu Boris Martinac Michael Sheetz 南方科技大学生物医学工程系深圳518055 Mechanobiology Institute National University of Singapore Victor Chang Cardiac Research Institute NSW 2010Australia Equipe Communication Intercellulaire et Infections Microbiennes Centre de Recherche Interdisciplinaire en Biologie(CIRB)Collège de FranceFrance Department of Biological Sciences National University of Singapore Molecular MechanoMedicine Program Dept.of Biochemistry and Molecular BiologyUniversity of Texas Medical BranchUSA

目的近十年来新发现广泛表达于多种组织细胞中的力敏感钙离子通道Piezo1在触觉/听觉感受,血压控制,免疫激活等生理过程中起到关键的力学感知作用。经典模型认为,Piezo1在细胞膜上扩散分布并在细胞膜局部表面张力起伏的作用下被激活,但... 详细信息

目的近十年来新发现广泛表达于多种组织细胞中的力敏感钙离子通道Piezo1在触觉/听觉感受,血压控制,免疫激活等生理过程中起到关键的力学感知作用。经典模型认为,Piezo1在细胞膜上扩散分布并在细胞膜局部表面张力起伏的作用下被激活,但其在细胞复杂生理过程中的调控还有很多未知问题尚待回答。方法通过一系列显微成像方法研究了Piezo1的时空分布与活性在不同种类细胞中的力学调控。

关键词：钙离子通道细胞转化力学调控免疫激活血压控制组织细胞经典模型未知问题

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南海北部晚第四纪颗石重量变化及其环境控制因素

南海北部晚第四纪颗石重量变化及其环境控制因素

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中国古生物学会化石藻类专业委员会第十九次学术年会、中国古生物学会微体学分会第十一届会员代表大会暨第十八次学术年会、江苏省古生物学会2020年学术年会

作者：苏翔刘传联 Luc Beaufort 中国科学院边缘海与大洋地质重点实验室中国科学院南海海洋研究所同济大学海洋地质国家重点实验室 Aix Marseille University CNRSIRDINRAColl FranceCEREGE

颗石藻是一类海洋单细胞钙质超微浮游植物,能够分泌形成覆盖在细胞表面的微小盘状碳酸钙"鳞片",称为颗石。由于其独特的生理生态功能,颗石藻已成为研究海洋酸化对生物影响的重要的实验对象。然而对于颗石藻钙化作用如何响应大气CO2浓度... 详细信息

颗石藻是一类海洋单细胞钙质超微浮游植物,能够分泌形成覆盖在细胞表面的微小盘状碳酸钙"鳞片",称为颗石。由于其独特的生理生态功能,颗石藻已成为研究海洋酸化对生物影响的重要的实验对象。然而对于颗石藻钙化作用如何响应大气CO2浓度变化,室内培养实验的结论并不一致,还需从自然状态下探索颗石藻钙化作用的变化规律。本研究选取南海北部钙质化石保存完好的沉积物柱状样MD05-2904孔,对其中颗石的形态重量进行分析,

关键词：南海北部控制因素颗石藻晚第四纪

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