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Analysis of the in vitro degradation and the in vivo tissue response to bi-layered 3D-printed scaffolds combining PLA and biphasic PLA/bioglass components--Guidance of the inflammatory response as basis for osteochondral regeneration

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Bioactive Materials 2017年第4期2卷 208-223页

作者： Mike Barbeck Tiziano Serra Patrick Booms Sanja Stojanovic Stevo Najman Elisabeth engel Robert Sader Charles James Kirkpatrick Melba Navarro Shahram Ghanaati Private Office BerlinGermany Institute for Bioengineering of Catalonia(IBEC) Biomaterials for Regenerative MedicineBarcelonaSpain Clinic of Oro-Maxillofacial and Plastic Surgery FORM-LabGoethe University FrankfurtFrankfurtGermany University of Ni's Faculty of MedicineDepartment for Cell and Tissue EngineeringInstitute of Biology and Human GeneticsNi'sSerbia Technical University of Catalonia(UPC) Dpt.Materials Science and MetallurgySpain ciber en bioingenieria Biomateriales y Nanomedicina(CIBER-BBN)Spain

The aim of the present study was the in vitro and in vivo analysis of a bi-layered 3D-printed scaffold combining a PLA layer and a biphasic PLA/bioglass G5 layer for regeneration of osteochondral defects in vivo Focus of the in vitro analysis was on the(molecular)weight loss and the morphological and mechanical variations after immersion in *** in vivo study focused on analysis of the tissue reactions and differences in the implant bed vascularization using an established subcutaneous implantation model in CD-1 mice and established histological and histomorphometrical *** scaffold parts kept their structural integrity,while changes in morphology were observed,especially for the PLA/G5 *** properties decreased with progressive degradation,while the PLA/G5 scaffolds presented higher compressive modulus than PLA *** tissue reaction to PLA included low numbers of BMGCs and minimal vascularization of its implant beds,while the addition of G5 lead to higher numbers of BMGCs and a higher implant bed *** revealed that the use of a bi-layered scaffold shows the ability to observe distinct in vivo response despite the physical proximity of PLA and PLA/G5 ***,the results showed that the addition of G5 enables to reduce scaffold weight loss and to increase mechanical ***,the addition of G5 lead to a higher vascularization of the implant bed required as basis for bone tissue regeneration mediated by higher numbers of BMGCs,while within the PLA parts a significantly lower vascularization was found optimally for chondral ***,this data show that the analyzed bi-layered scaffold may serve as an ideal basis for the regeneration of osteochondral tissue ***,the results show that it might be able to reduce the number of experimental animals required as it may be possible to analyze the tissue response to more than one implant in one experimental animal.

关键词： Bioactive glass Polylactic acid(PLA) Bi-layer scaffold Multinucleated giant cells Bone substitute Vascularization Calcium phosphate glass

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endosomal escape of protein nanoparticles engineered through humanized histidine-rich peptides

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Science China Materials 2020年第4期63卷 644-653页

作者： Hector Lopez-Laguna Rafael Cubarsi Ugutz Unzueta Ramon Mangues Esther Vazquez Antonio Villaverde Institut de Biotecnologia i de Biomedicina Universitat Aut6noma de BarcelonaBellaterra08193 BarcelonaSpain Departament de Genetica i de Microbiologia Universitat Aut6noma de BarcelonaBellaterra08193 BarcelonaSpain ciber de bioingenieria Biomateriales y Nanomedicina(CIBER-BBN)C/Monforte de Lemos 3-528029 MadridSpain Departament de Matematiques Universitat Politecnica de Catalunya08034BarcelonaSpain Institut d'Investigacions Biomediques Sant Pau Hospital de la Santa Creu i Sant Pau08025 BarcelonaSpain

Poly-histidine peptides such as H6(HHHHHH)are used in protein biotechnologies as purification tags,protein-assembling agents and endosomal-escape *** pleiotropic properties of such peptides make them appealing to design protein-based smart materials or nanoparticles for imaging or drug delivery to be produced in form of recombinant ***,the clinical applicability of H6-tagged proteins is restricted by the potential immunogenicity of these *** this study,we have explored several humanized histidine-rich peptides in tumor-targeted modular proteins,which can specifically bind and be internalized by the target cells through the tumoral marker *** were particularly interested in exploring how protein purification,self-assembling and endosomal escape perform in proteins containing the variant histidine-rich *** the tested candidates,the peptide H5 E(HEHEHEHEH)is promising as a good promoter of endosomal escape of the associated fulllength protein upon endosomal *** numerical modelling of cell penetration and endosomal escape of the tested proteins has revealed a negative relationship between the amount of protein internalized into target cells and the efficiency of cytoplasmic *** fact demonstrates that the His-mediated,proton sponge-based endosomal escape saturates at moderate amounts of internalized protein,a fact that might be critical for the design of protein materials for cytosolic molecular delivery.

关键词： protein materials nanoparticles genetic design endosomal escape poly-histidines

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Tools for the diagnosis of hepatitis C virus infection and hepatic fibrosis staging

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World Journal of Gastroenterology 2014年第13期20卷 3431-3442页

作者： Verónica Saludes Victoria González Ramon Planas Lurdes Matas Vicente Ausina Elisa Martró Institut d’ Investigació en Ciències de la Salut Germans Trias i Pujol Hospital Universitari Germans Trias i Pujol (Universitat Autònoma de Barcelona)08916 BadalonaSpain ciber Epidemiología y Salud Pública (ciberESP) 08003 BarcelonaSpain Centre for Epidemiological Studies on HIV/STI in Catalonia (CEEISCAT)-ICO 08916 BadalonaSpain Liver Unit Hospital Universitari Germans Trias i Pujol08916 BadalonaSpain Ramon Planas CIBER Enfermedades Hepáticas y Digestivas (CIBEREHD)08036 BarcelonaSpain ciber enfermedades Respiratorias (ciberES) 07110 BunyolaSpain

Hepatitis C virus(HCV)infection represents a major public health *** C can be cured bytherapy,but many infected individuals are unaware of their *** HCV screening,fast diagnosis and characterization,and hepatic fibrosis staging are highly relevant for controlling transmission,treating infected patients and,consequently,avoiding end-stage liver *** to HCV can be determined with high sensitivity and specificity with currently available third generation serology ***,the use of point-of-care tests can increase HCV screening ***,active HCV infection must be confirmed by direct diagnosis ***,HCV genotyping is required prior to starting any ***,high-volume clinical laboratories use different types of automated platforms,which have simplified sample processing,reduced hands-on-time,minimized contamination risks and human error and ensured full traceability of *** advances have also been made in the field of fibrosis stage assessment with the development of non-invasive methods,such as imaging techniques and serum-based ***,no single test is currently available that is able to completely replace liver *** review focuses on approved commercial tools used to diagnose HCV infection and the recommended hepatic fibrosis staging tests.

关键词： Hepatitis C virus Diagnosis Real-time polymerase chain reaction Serology Hepatitis C virus-RNA quantification Hepatitis C virus genotyping Hepatic fibrosis staging

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Ocular findings in Zucker Diabetic Fatty rats emphasize the key role of neuroglia degeneration in diabetic retinopathy pathophysiology

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Neural Regeneration Research 2018年第2期13卷 239-240页

作者： ivan fernandez-bueno yolanda diebold instituto universitario de oftalmobiología aplicada university of valladolidValladolidSpain red temática de investigación cooperativa en salud(retics) oftaredinstituto de salud carlos IIIMadridSpain biomedical research networking center on bioengineering biomaterials and nanomedicineciber-bbnValladolidSpain

Diabetes mellitus is a leading cause of acquired vision loss and one of the world＇s fastest growing chronic diseases. Diabetic retinopathy （DR）, a specific complication of chronic hyperglycemia, is the leading cause of acquired vision loss worldwide in middle-aged and there- fore economically active people that also increases the medical and economic burden on the society （Klein, 2007）.

关键词： Ocular findings in Zucker Diabetic Fatty rats emphasize the key role of neuroglia degeneration in diabetic retinopathy pathophysiology

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Emotional evaluation of lighting in university classrooms:A preliminary study

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Frontiers of Architectural Research 2018年第4期7卷 600-609页

作者： Nuria Castilla Carmen Llinares Fabio Bisegna Vicente Blanca-Gimenez Dpto.Construcciones Arquitectonicas Universitat Politecnica de ValenciaCamino de Veras/nValencia 46022Spain Instituto de Investigacion e Innovacion en bioingenieria i3BUniversitat Politecnica de ValenciaCamina de Veras/nValencia 46022Spain Dept.of Astronautical Electrical and Energetic Engineering (DIAEE)SAPIENZA University of RomeVia Eudossiana 18Rome 00184Italy

In educational environments an improvement in the quality of interior lighting has a direct benefit in increasing productivity and alertness of students and teachers, as well as very important implications for the energy efficiency of the education facilities but when a replacement with different lighting is analyzed, research may be tarnished by users’ pre-formed opinions, influenced by manufacturers and advertising. Consequently, it is necessary to understand the users’ point of view, even before of being subjected to any change in the lighting *** on the Kansei engineering framework, the general objective of this paper is to evaluate and compare the subjective evaluation of students’ pre-formed opinions to lighting provided by two types of lamps (fluorescent and LED). The subjective assessment of 427 university students has been compared over four years. The results show significant differences in students’ subjective evaluation. This finding highlights the existence of symbolic or functional attributes of the usefulness perceived by the student that could influence on investigations in which different types of lighting are compared.

关键词： Kansei engineering Lighting Fluorescent LED Subjective assessment University classroom

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Insight into endoplasmic reticulum-mitochondria contacts in human amyotrophic lateral sclerosis

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Neural Regeneration Research 2024年第7期19卷 1407-1408页

作者： Naomi Hartopp Andrea Markovinovic Christopher CJ Miller Patricia Gomez-Suaga Sheffield Institute for Translational Neuroscience University of SheffieldSheffieldUK Department of Basic and Clinical Neuroscience Institute of PsychiatryPsychology and NeuroscienceLondonUK Universidad de Extremadura.Departamento de Bioquímica y Biología Molecular y Genética Facultad de Enfermería y Terapia OcupacionalCáceresSpain Centro de Investigación Biomédica en Red en enfermedades Neurodegenerativas-Instituto de Salud Carlos Ⅲ(ciber-ciberNED-ISCⅢ) MadridSpain Instituto Universitario de Investigación Biosanitaria de Extremadura(INUBE) CáceresSpain

Amyotrophic lateral sclerosis(ALS) is a fastprogressing fatal neurodegenerative disease and the most common form of motor neuron *** is currently no cure and approximately 90% of cases are *** shares genetic causes,clinical and neuropathological features with frontotemporal dementia,the second most common form of presenile *** and frontotemporal dementia are therefore considered a disease spectrum(Abramzon et al.,2020).

关键词： senile sclerosis

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基于临床的普通人群中孤立性肺结节的患病率及其变量:一项横断面研究

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国际医学放射学杂志 2014年第6期 596-597页

作者： N.Gómez-Sáez I.González-lvarez J.Vilar I.Hernández-Aguado M.L.Domingo M.F.Lorente 王玉婷 ciber en Epidemiologíay Salud Pública BarcelonaSpain

摘要目的确定胸部放射学检查中的孤立性肺结节(SPN)患病率以及在非高风险临床人群中与恶性肿瘤相关的特征。方法 2010年—2011年期间,年龄35周岁及以上的病

关键词：肺结节孤立性患病率

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Biomolecular functionalization for enhanced cell–material interactions of poly(methyl methacrylate)surfaces 5th China-Europe Symposium on Biomaterials in Regenerative Medicine(CESB 2015)Hangzhou,China April 7–10,2015

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Regenerative Biomaterials 2015年第3期2卷 167-175页

作者： Xavier Punet Rodolphe Mauchauffe´ Jose´C.Rodrı´guez-Cabello Matilde Alonso Elisabeth engel Miguel A.Mateos-Timoneda Biomaterials for Regenerative Therapies Group Institute for Bioengineering of Catalonia(IBEC)Barcelona 08028Spain ciber en Bioingenerı´a Biomateriales y Nanomedicina(CIBER-BBN)Spain G.I.R.Bioforge Universidad Valladolid(UVA)Valladolid 47011Spain Department of Material Science and Metallurgical engineering Technical University of Catalonia(UPC)Barcelona 08028Spain

The integration of implants or medical devices into the body tissues requires of good cell–material ***,most polymeric materials used for these applications lack on biological cues,which enhanced mid-and long-term implant failure due to weak integration with the surrounding *** used strategies for tissue–material integration focus on functionalization of the material surface by means of natural proteins or short ***,the use of these biomolecules involves major drawbacks such as immunogenic problems and oversimplification of the ***,designed elastin-like recombinamers(ELRs)are used to enhance poly(methyl methacrylate)surface properties and compared against the use of short *** this study,cell response has been analysed for different functionalization conditions in the presence and absence of a competing protein,which interferes on surface–cell interaction by unspecific adsorption on the *** study has shown that ELRs can induce higher rates of cell attachment and stronger cell anchorages than short peptides,being a better choice for surface functionalization.

关键词： cell adhesion elastin-like recombinamer poly(methyl methacrylate) PMMA RGD peptide surface modification

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Human adipose derived stem cells are superior to human osteoblasts (HOB) in bone tissue engineering on a collagen-fibroin-ELR blend

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Bioactive Materials 2017年第2期2卷 71-81页

作者： Esen Sayin Rosti Hama Rashid JoséCarlos Rodríguez-Cabello Ahmed Elsheikh Erkan Türker Baran Vasif Hasirci METU Department of BiotechnologyAnkaraTurkey BIOMATen METU Center of Excellence in Biomaterials and Tissue EngineeringDumlupinar Blvd No:106800 AnkaraTurkey University of Liverpool School of EngineeringL693GH LiverpoolUK BIOFORGE CIBER-BBNCampus“Miguel Delibes”Edificio LUCIAUniversidad de ValladolidPaseo Belen 1947011 ValladolidSpain METU Department of Biological SciencesAnkara06800Turkey

The ultrastructure of the bone provides a unique mechanical strength against compressive, torsional andtensional stresses. An elastin-like recombinamer (ELR) with a nucleation sequence for hydroxyapatitewas incorporated into films prepared from a collagen-silk fibroin blend carrying microchannel patternsto stimulate anisotropic osteogenesis. SEM and fluorescence microscopy showed the alignment ofadipose-derived stem cells (ADSCs) and the human osteoblasts (HOBs) on the ridges and in the groovesof microchannel patterned collagen-fibroin-ELR blend films. The Young's modulus and the ultimatetensile strength (UTS) of untreated films were 0.58 ± 0.13 MPa and 0.18 ± 0.05 MPa, respectively. After 28days of cell culture, ADSC seeded film had a Young's modulus of 1.21 ± 0.42 MPa and UTS of0.32 ± 0.15 MPa which were about 3 fold higher than HOB seeded films. The difference in Young'smodulus was statistically significant (p: 0.02). ADSCs attached, proliferated and mineralized better thanthe HOBs. In the light of these results, ADSCs served as a better cell source than HOBs for bone tissueengineering of collagen-fibroin-ELR based constructs used in this study. We have thus shown theenhancement in the tensile mechanical properties of the bone tissue engineered scaffolds by usingADSCs.

关键词： Adipose-derived stem cells Human osteoblasts Tissue engineering Bone Mechanical properties

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Templated Colloidal Self-Assembly for Lattice Plasmon engineering

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Accounts of Materials Research 2021年第9期2卷 816-827页

作者： Leonardo Scarabelli David Vila-Liarte Agustín Mihi Luis M.Liz-Marzán Institute of Materials Science of Barcelona ICMAB-CSIC08193 BellaterraSpain CIC biomaGUNE Basque Research and Technology Alliance(BRTA)20014 Donostia-San SebastiánSpain Centro de Investigación Biomédica en Red BioingenieríaBiomateriales y Nanomedicina(CIBER-BBN)20014 Donostia-San SebastiánSpain Ikerbasque Basque Foundation for Science48013 BilbaoSpain

CONSPECTUS:Over the past 30 years,the engineering of plasmonic resonances at the nanoscale has progressed dramatically,with important contributions in a variety of different fields,including chemistry,physics,biology,engineering,and ***,heavy optical losses related to the use of noble metals for the fabrication of plasmonic structures hindered their application in various ***,an answer to these long-lasting issues emerged in the use of lattice plasmon resonances(LPRs,also called surface lattice resonances),bringing new excitement in the field of ***,the organization of plasmonic nanoparticles into ordered arrays enables far-field coupling of the scattered light exploiting the diffraction modes of the array,generating plasmonic resonances with bandwidths as narrow as a few nanometers,corresponding to an increase of over 10-fold in the quality factors compared to localized plasmon *** such,LPRs offer new opportunities to harness light−matter interactions at the nanoscale,while generating renewed interest in the self-assembly of colloidal metal nanoparticles,as a scalable approach to the preparation of such plasmonic *** self-assembly emerged as one of the most versatile approaches,being compatible with soft-lithographic techniques such as nanoimprint lithography and amenable to a variety of materials,colloids,and *** self-assembly additionally allows the preparation of arrays where the repeating units are composed of multiple self-assembled nanoparticles(i.e.,plasmonic clusters).In this system,near-field coupling can be finely tuned,thereby showing promising results in biosensing,catalysis,or plasmonic *** this Account,we review the preparation of ordered arrays of clusters of plasmonic *** present various aspects involved in the templated self-assembly of colloidal nanoparticles,with the aim of achieving at the same time close-packed structures within each cavity

关键词： coupling enable versatile

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