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A facile method to fabricate cell-laden hydrogel microparticles of tunable sizes using thermal inkjet bioprinting

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Droplet 2024年第4期3卷 29-38页

作者： Ratima Suntornnond Wei Long Ng Viktor Shkolnikov Wai Yee Yeong HP-NTU Digital Manufacturing corporate Lab Nanyang Technological University(NTU)Singapore Singapore centre for 3D Printing(Sc3DP) School of Mechanical and Aerospace EngineeringNanyang Technological University(NTU)Singapore HP inc. Palo AltoCaliforniaUSA

This study investigates the application of a drop-on-demand(DOD)thermal inkjet(TIJ)-based bioprinting system for the fabrication of cell-laden hydrogel microparticles(HMPs)with tunable *** TIJ bioprinting technique involves the formation of vapor bubbles within the print chamber through thermal energy,expelling small droplets of bio-ink onto a *** study employs a heat-treated saponified gelatin-based bio-ink,*** bio-ink is modified through methacrylic anhydride functionalization and undergoes subsequent saponification and heat treatment *** concentrations of SPAN 80 surfactant in mineral oil were evaluated to assess their influence on HMP size and *** results indicate a direct correlation,with higher SPAN 80 concentrations resulting in smaller and more stable *** study further investigates the influence of jetting volume on HMP size distribution,revealing that larger jetting volumes lead to increased HMP sizes,attributed to droplet *** is supported by our further study via a Monte carlo simulation,which shows that the mean droplet diameter grows approximately linear with the number of dispensed *** addition,the study demonstrates the capability of the TIJ bioprinting system to achieve multimaterial encapsulation within HMPs,exemplified by staining living cells with distinct cytoplasmic membrane *** presented approach provides insights into the controlled fabrication of cell-laden HMPs,highlighting the versatility of the TIJ bioprinting system for potential applications in tissue engineering and drug delivery.

关键词： printing thermal sizes

Urban rail transit disruption management: Research progress and future directions

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Frontiers of Engineering Management 2024年第1期11卷 79-91页

作者： Lebing WANG jian Gang JIN Lijun SUN Der-Horng LEE School of Naval Architecture Ocean&Civil Engineeringand State Key Laboratory of Ocean EngineeringShanghai Jiao Tong UniversityShanghai 200240China Department of civil Engineering McGill UniversityMontrealQuebec H3A 0C3Canada ZJU-UIUc Institute Zhejiang UniversityHaining 314400China

Urban rail transit (URT) disruptions present considerable challenges due to several factors: i) a high probability of occurrence, arising from facility failures, disasters, and vandalism;ii) substantial negative effects, notably the delay of numerous passengers;iii) an escalating frequency, attributable to the gradual aging of facilities;and iv) severe penalties, including substantial fines for abnormal operation. This article systematically reviews URT disruption management literature from the past decade, categorizing it into pre-disruption and post-disruption measures. The pre-disruption research focuses on reducing the effects of disruptions through network analysis, passenger behavior analysis, resource allocation for protection and backup, and enhancing system resilience. conversely, post-disruption research concentrates on restoring normal operations through train rescheduling and bus bridging services. The review reveals that while post-disruption strategies are thoroughly explored, pre-disruption research is predominantly analytical, with a scarcity of practical pre-emptive solutions. Moreover, future research should focus more on increasing the interchangeability of transport modes, reinforcing redundancy relationships between URT lines, and innovating post-disruption strategies.

关键词： urban rail transit disruption management resilient network train rescheduling bus bridging services

changes in microorganisms and metabolites during long-term fermentation of commercially manufactured anchovy sauce

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Food Science and Human Wellness 2024年

作者： Daehyeon Kim Seong-Eun Park Eun-Ju Kim Seung-Ho Seo Kwang-Moon cho Sun Jae Kwon Hong-Seok Son Department of Biotechnology， college of Life Sciences and Biotechnology， Korea University Sonlab inc. 3. AccuGene inc.

This study was performed to investigate the changes in microbial communities and metabolites during the long-term fermentation of commercially manufactured anchovy sauce. Samples of commercial anchovy sauce were collected from large-scale fermentation tanks with fermentation periods of up to 7.87 years. The complex bacterial community was simplified to two genera， Tetragenococcus and Halanaerobium， after approximately 0.55 years of fermentation. Although genera， such as Saccharomyces， cladosporium， candida， and Aspergillus， were relatively dominant， no clear pattern was identified in fungal community analysis. The longitudinal metabolite profile demonstrated that approximately half (55.8%) of the metabolites present in anchovy sauce were produced within a year of fermentation due to rapid fermentation. Despite the static microbial community， the contents of several metabolites including amino acids and biogenic amines changed continuously during the long-term fermentation of anchovy sauce. This study provides novel insights into the changes in microbiota and metabolites in fish sauce produced without any starter inoculation.

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An all-in-one FeO_(x)-rGO sponge fabricated by solid-phase microwave thermal shock for water evaporation and purification

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Journal of Environmental Sciences 2024年第4期138卷 671-683页

作者： Youkun Sun Xiuwen Zhao Xueling Song Jinchen Fan Junhe Yang Yingchun Miao Shuning Xiao School of Materials and chemistry University of Shanghai for Science and TechnologyShanghai 200093China college of chemistry and Environmental Science Qujing Normal UniversityQujing 655011China Prytula Igor collaborate Innovation center for Diamond Shanghai Jian Qiao UniversityShanghai 201306China

Developing high-efficiency photothermal seawater desalination devices is of significant importance in addressing the shortage of *** much effort made into photothermal materials,there is an urgent need to design a rapidly synthesized photothermal evaporator for the comprehensive purification of complex ***,we report on all-in-one FeOx-rGO photothermal sponges synthesized via solid-phase microwave thermal *** narrow band gap of the semiconductor material Fe_(3)O_(4) greatly reduces the recombination of electron-hole pairs,enhancing non-radiative relaxation light *** abundantπorbitals in rGO promote electron excitation and thermal vibration between the *** of the surface hydrophilicity and hydrophobicity promotes salt resistance while simultaneously achieving the purification of various complex polluted *** optimized GFM-3 sponge exhibitedan enhanced photothermal conversion rate of 97.3% and a water evaporation rate of 2.04 kg/(m^(2)·hr),showing promising synergistic water purification *** findings provide a highly efficient photothermal sponge for practical applicationsof seawater desalination and purification,as well as develop a super-rapid processing methodology for evaporation devices.

关键词： Desalination Photothermal device Microwave synthesis Water purification FeO_(x)-rGO

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基于交通波特征的车道级车流溯源方法

交通运输系统工程与信息 2024年第1期24卷 159-167页

作者：袁见刘福强安琨郑喆马万经俞秋田同济大学道路与交通工程教育部重点实验室上海201804 麦吉尔大学土木工程系加拿大蒙特利尔H3A0C3 浙江数智交院科技股份有限公司杭州310000

为支撑城市道路中低渗透率轨迹数据条件下车流溯源分析,本文提出一种基于交通波特征的车道级车流溯源方法。基于NGSIM(Next Generation Simulation)数据集中的真实车辆轨迹信息,解析不同来源车流的交通波特征差异性;结合信号配时方案,... 详细信息

为支撑城市道路中低渗透率轨迹数据条件下车流溯源分析,本文提出一种基于交通波特征的车道级车流溯源方法。基于NGSIM(Next Generation Simulation)数据集中的真实车辆轨迹信息,解析不同来源车流的交通波特征差异性;结合信号配时方案,从车辆初次停车时刻、集结波起始时空位置、集结波斜率、集结波覆盖长度等多个维度验证了交通波应用于车流溯源的可行性。在此基础上,提取集结波5项特征参数,构建4种基于机器学习的车道级车流实时溯源模型。采用NGSIM数据对模型参数进行训练标定,并对不同归一化方法、不同数据量、不同数据精度下的模型效果进行灵敏性分析。结果表明,在低数据量场景下,特征参数宜采用Min-Max法进行归一化处理,溯源流量平均误差比例最大不超过23.60%;当数据量较为充分时(超过100个信号周期),特征参数宜采用Z-Score法进行归一化处理,平均误差比例最大不超过9.90%,效果最佳的梯度提升回归模型的平均误差低至0.01%。此外,数据误差对不同模型的影响有所差异,但在误差较大时模型不会出现失效问题。本文所构建方法不依赖于固定检测器数据,未来可进一步研究交通波在网络层面的溯源方法。

关键词：智能交通车流时空溯源机器学习车辆轨迹交通波

Assessing efficacy of standard impregnation techniques on die-cast aluminum alloys using X-ray micro-cT

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china Foundry 2024年第3期21卷 276-286页

作者： Ajith Bandara Koichi Kan Katanaga Yusuke Natsuto Soga Akifumi Koike Toru Aoki Hamamatsu TSc Chuo Hatsumei Institute322 lida-choMinami-kuHamamatsu435-0028Japan Graduate School of Medical Photonics ShizuokaUniversityJohoku 3-5-1HamamatsuShizuoka432-8561Japan ANSeeN inc Johoku 3-5-1HamamatsuShizuoka432-8561Japan Research Institute of Electronics ShizuokaUniversityJohoku 3-5-1HamamatsuShizuoka432-8561Japan

Utilizing lightweight Al alloys in various industrial applications requires achieving precise pressure tightness and leak *** pressure impregnation(VPI)with thermosetting polymers is commonly used to address leakage defects in die-cast Al *** this study,the efficacy of the VPI technique in sealing alloy parts was investigated using a combination of nondestructive micro X-ray computed tomography(micro XcT)and a standard leak *** results demonstrate that the commonly used water leak test is insufficient for determining the sealing ***,micro XcT shows distinct advantages by enabling more comprehensive *** reveals the presence of a low atomic number impregnates sealant within casting defects,which has low grey contrast and allows for visualizing primary leakage paths in *** effective atomic number of impregnated resin is 6.75 and that of Al alloy is 13.69 by dual-energy X-ray *** research findings will contribute to enhancing the standard VPI process parameters and the properties of impregnating sealants to improve quality assurance for impregnation in industrial metals.

关键词： nondestructive testing Al alloy die-casting vacuum pressure impregnation micro X-ray computed tomography duel-energy X-ray cT

Development and evaluation of a safe and effective sugar-free herbal lollipop that kills cavity-causing bacteria

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International Journal of Oral Science 2011年第1期3卷 13-20页

作者： chu-hong Hu jian He Randal Eckert Xiao-yang Wu Li-na Li Yan Tian Renate Lux Justin A. Shuffer Faina Gelman Janet Mentes Sue Spackman Janet Bauer Maxwell H. Anderson Wen-yuan Shi School of Dentistry University of California Los Angeles USA c3 jian inc Inglewood USA UcLA School of Nursing University of California Los Angeles USA

Dental caries （tooth decay） is caused by a specific group of cariogenic bacteria, like Streptococcus mutans, which convert dietary sugars into acids that dissolve the mineraI in tooth structure. Killing cariogenic bacteria is an effective way to control or prevent tooth decay. In a previous study, we discovered a novel compound （Glycyrrhizol A）, from the extraction of licorice roots, with strong antimicrobial activity against cariogenic bacteria. In the current study, we developed a method to produce these specific herbal extracts in large quantities, and then used these extracts to develop a sugar-free lollipop that effectively kills cariogenic bacteria like Streptococcus mutans. Further studies showed that these sugar-free lollipops are safe and their antimicrobial activity is stable. Two pilot human studies indicate that a brief application of these lollipops （twice a day for ten days） led to a marked reduction of cariogenic bacteria in oral cavity among most human subjects tested. This herbal lollipop could be a novel tool to promote oral health through functional foods.

关键词： antimicrobial therapy licorice Streptococcus mutans

Robust Machine Learning Mapping of sEMG Signals to Future Actuator commands in Biomechatronic Devices

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Journal of Bionic Engineering 2024年第1期21卷 270-287页

作者： Ali Nasr Sydney Bell Rachel L.Whittaker clark R.Dickerson John McPhee Systems Design Engineering University of WaterlooWaterloo N2L 3G1Canada Kinesiology and Health Sciences University of WaterlooWaterloo N2L3G3Canada

A machine learning model for regression of interrupted Surface Electromyography(sEMG)signals to future control-oriented signals(e.g.,robot’s joint angle and assistive torque)of an active biomechatronic device for high-level myoelectric-based hierarchical control is proposed.A Recurrent Neural Network(RNN)was trained using output data,initially obtained from offline optimization of the biomechatronic(human–robot)device and shifted by the prediction *** input of the RNN consisted of interrupted sEMG signals(to mimic signal disconnections)and previous kinematic signals of the assistive *** RNN with a 0.1-s prediction horizon could predict the control-oriented joint angle and assistive torque with 92%and 86.5%regression accuracy,respectively,for the test *** proposed approach permits a fast,predictive,and direct estimation of control-oriented signals instead of an iterative process that optimizes assistive torque in the inverse dynamic simulation of a multibody human–robot *** with these interrupted input signals significantly improves the regression accuracy in the case of sEMG signal *** Robust Predictive control-oriented Machine Learning(Robust-MuscleNET)model can support volitional high-level myoelectric-based control of biomechatronic devices,such as exoskeletons,prostheses,and assistive/resistive *** work should study the application to prosthesis control as well as the repeatability of the high-level controller with electrode *** low-level hierarchical controller that manages the human–robot interaction,the assistance/resistance strategy,and the actuator coordination should also be studied.

关键词： Myoelectric-based control-Surface electromyography Machine learning Multibody system dynamics Exoskeleton Bionic

Research on the Intervention Effect of Vibroacoustic Therapy in the Treatment of Patients with Depression

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International Journal of Mental Health Promotion 2024年第2期26卷 149-160页

作者： Xiaodan Wang Zheng Xie Guiping Du School of Education Xuchang UniversityXuchang461000China Department of Psychological Medicine Henan Provincial People’s HospitalZhengzhou463599China Department of clinical Psychology Xuchang Jian’an HospitalXuchang461000China

Research purpose:This study implemented somatosensory music therapy on patients with depressive disorders,and explored the effects of somatosensory music therapy on the degree of depression,positive and negative emotions,intuitive stress and autonomic nervous function of *** method:We collected 66 patients diagnosed with depression from the Department of Psychological Medicine of Henan Provincial People’s Hospital,and divided them into a control group and an intervention group according to the random number table,with 33 people in each *** control group received routine psychiatric treatment and *** the basis of the control group,the intervention group received somatosensory music therapy 3 times a week,30 min each time,for 4 consecutive *** Hamilton Depression Scale,Positive and Negative Emotion Scale,Perceived Stress Scale,and Heart Rate Variability Index were collected and analyzed at baseline(T0),2 weeks after treatment(T1)and 4 weeks after treatment(T2).Research results:After 4 weeks of treatment,the HAMD scores of the two groups decreased to varying degrees,and the difference between the groups was statistically significant(pcores of positive emotions in both groups increased to varying degrees,and the scores of negative emotions increased to varying *** scores decreased to varying degrees,and there were statistically significant differences between the positive and negative emotion groups(pcPSS scores of the two groups decreased to varying degrees,and the difference between the two groups was statistically significant(pct of intervention factors alone,after 4 weeks of treatment,SDNN and RMSSD indexes all increased to varying degrees,and the degree of increase in the intervention group was greater than that in the control group,and the difference between the groups was statistically significant(pch con

关键词： Somatosensory music depressive disorder intervention effect perceived stress heart rate variability