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Fungal diversity in adult date palm(Phoenix dactylifera L.) revealed by culture-dependent and culture-independent approaches

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2013年第12期14卷 1084-1099页

作者： Ines BEN CHOBBA Amine ELLEUCH Imen AYADI Lamia KHANNOUS Ahmed NAMSI Frederique CERQUEIRA Noureddine DRIRA Néji gHARSALLAH Tatiana VALLAEYS Laboratoire de Biotechnologies Végétales Appliquées à l'Amélioration des Cultures LBVAAC Faculté des Sciences de SfaxB.P. 1171 3000 Sfax Tunisia UMR 5119 Université de Montpellier 2 Sciences et Techniques pl E. BataillonCC093 34095 Montpellier France Laboratoire de Microorganismes et de Biomolécules Centre de Biotechnologie de Sfax Université de Sfax Route Sidi MansourB.P. 1177 3018 Sfax Tunisia Laboratoire de Phytopathologie Oasienne Centre Régional de Recherches en Agriculture Oasienne à DegacheDegache 2260 Tunisia Plateforme Séquen age-génotypage SFR "Montpellier Environnement Biodiversité" ISEM Université de Montpellier 234095 Montpellier France

Endophytic flora plays a vital role in the colonization and survival of host plants, especially in harsh environments, such as arid regions. This flora may, however, contain pathogenic species responsible for various troublesome host diseases. The present study is aimed at investigating the diversity of both cultivable and non-cultivable endophytic fungal floras in the internal tissues(roots and leaves) of Tunisian date palm trees(Phoenix dactylifera). Accordingly, 13 isolates from both root and leaf samples, exhibiting distinct colony morphology, were selected from potato dextrose agar(PDA) medium and identified by a sequence match search wherein their 18S–28S internal transcribed spacer(ITS) sequences were compared to those available in public databases. These findings revealed that the cultivable root and leaf isolates fell into two groups, namely Nectriaceae and Pleosporaceae. Additionally, total DNA from palm roots and leaves was further extracted and ITS fragments were amplified. restriction fragment length polymorphism(RFLP) analysis of the ITS from 200 fungal clones(leaves: 100; roots: 100) using HaeIII restriction enzyme revealed 13 distinct patterns that were further sequenced and led to the identification of Alternaria, Cladosporium, Davidiella(Cladosporium teleomorph), Pythium, Curvularia, and uncharacterized fungal endophytes. Both approaches confirmed that while the roots were predominantly colonized by Fusaria(members of the Nectriaceae family), the leaves were essentially colonized by Alternaria(members of the Pleosporaceae family). Overall, the findings of the present study constitute, to the authors' knowledge, the first extensive report on the diversity of endophytic fungal flora associated with date palm trees(P. dactylifera).

关键词： Date palm tree(Phoenix dactylifera) Endophytic cultivable fungi rDNA internal transcribed spacer(ITS) Phylogenetic analysis Total DNA diversity analysis

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Identification of Microorganisms in Poultry Farms in N’djamena and the Border Areas of Hadjer-Lamis and Chari-Baguirmi Chad

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Journal of Agricultural Chemistry and Environment 2024年第2期13卷 223-234页

作者： Abakar Abbo Zakaria Bebanto Antipas Ban-Bo Nadine Terei Bongo Naré Richard gandolo Abdelsalam Adoum Doutoum Labortoire de Rattachement IRED (Institut de recherches en Elevage pour le Dé veloppement) University of N&#8217Djamena N&#8217Djamena Chad Laboratory of Biochemistry-Cellular and Molecular Biology-Microbiology (L2BCM) Faculty of Exact and Applied Sciences University of N&#8217Djamena N&#8217Djamena Chad Livestock research Institute for development (IRED) N&#8217Djamena Chad Adam Barka University of Abé ch&#233 Ab&#233ch&#233 Chad

Introduction: On the outskirts of Ndjamena, semi-industrial poultry farming and traditional poultry farming are practised informally on almost all poultry farms in Chad. This type of poultry farming is faced with real health problems attributable to a lack of monitoring of the vaccination schedule, inadequate compliance with biosecurity measures and poor application of the Ichikawa rule based on the 5 M’s. Objective: The aim of this article is to identify the microorganisms responsible for contamination of poultry farms in the study area. Method: The study was carried out from 28/04/2022 to 31/01/2023 on the basis of 300 samples taken from feed, drinking water, droppings and scrapings from poultry housing surfaces in the 30 farms that served as a framework for our research. Sampling was of the simple random type, and farms were selected on the basis of the farmers’ consent. The data were recorded on pre-established survey forms. Our study was cross-sectional, descriptive and prospective. Bacteria were isolated using the reference method NF EN ISO 6579 for Salmonella spp. and cultured on the specific medium eosin methylene blue (EMB) for Escherichia coli, Pseudomonas and Citrobacter freundii. results: The following results emerged from this study: Escherichia coli (5.33%), Pseudomonas (1.33%), Citrobacter freundii (12%) and Salmonella paratyphi (21.68%). Conclusion: Of the 300 samples analysed, 121 (40.33%) were contaminated with pathogens. This high level of contamination is a health problem. The study shows that biosecurity is less satisfactory on the farms visited. Nevertheless, farms with a very satisfactory level of biosafety ensure food safety and variety for the population.

关键词： Microorganisms Identification Poultry Farms N’Djamena Hadjer-Lamis Chari-Baguirmi(Chad)

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Protein Profiles of Pod Borer Maruca resistant Transgenic Cowpea

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American Journal of Plant Sciences 2023年第12期14卷 1453-1463页

作者： Mounyratou Rabo Teyioue Benoit Joseph Batieno Assita Traoré-Barro Salimata Traoré Orokia Coulibaly Aboubacar Toguyeni Chantal Kaboré-Zoungrana Oumar Traoré Laboratoire National de Biosécurité Agence Nationale de Biosécurité (ANB) Ouagadougou Burkina Faso Institut de l’Environnement et recherches Agricoles Département Productions Végétales Ouagadougou Burkina Faso Laboratoire d’etudes et de recherches sur les ressources Naturelles et les Sciences de l’Environnement (LERNSE) Université Nazi BONI Bobo-Dioulasso Burkina Faso Laboratoire de génétique et amélioration des plantes (LAgAMEP) Institut de l’Environnement et Recherches Agricoles Ouagadougou Burkina Faso Laboratoire de protection et défense des cultures (LAPROdeC) Institut de l’Environnement et Recherches Agricoles Ouagadougou Burkina Faso

The grain legume cowpea Vigna unguiculata (L.) Walp. is a major protein source used for food and feed in Sub-Saharan Africa. The crop is affected by the pod borer Maruca vitrata against which transgenic lines were developed as part of the genetic control approach. This study aimed to assess the protein profiles in seeds and leaves of transgenic cowpea lines and their non-transgenic near-isogenic counterparts. Crude protein content was determined by the Kjeldahl method, and soluble proteins were quantified using Bradford dye binding assay. The average crude protein content ranged between 21.61% and 26.58% in the seeds and between 10.86% and 17.90% in the leaves. Total solubility varied between 13.03% and 20.64%. Osborne’s protein fractions contents in the seeds were 52.41% - 69.52% (albumin), 4.62% - 7.19% (globulin), 7.95% - 11.40% (glutelin) and 3% - 4% (prolamin). In any case, protein content differed significantly between cowpea genotypes but not between pairs of transgenic/non-transgenic lines. Insecticidal Cry1Ab protein expressed by transgenic lines was only detected in the albumin and globulin fractions. Altogether, these findings enhance our understanding of the effects of genetic modification on cowpea protein content and composition, with potential implications for nutritional and safety assessments.

关键词： Cowpea Protein Cry1Ab Protein Fractions

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Trifluoromethyl radical triggered radical cyclization of N-benzoyl ynamides leading to isoindolinones

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Science China Chemistry 2019年第11期62卷 1542-1546页

作者： Maud Cassé Christian Nisole Héloise Dossmann Yves gimbert Jean-Marie Fourquez Laure Haberkorn Cyril Ollivier Louis Fensterbank Sorbonne Université CNRSInstitut Parisien de Chimie MoléculaireIPCM75005 ParisFrance Institut de recherches Servier 78290 Croissy-sur-SeineFrance Département de Chimie Moléculaire CNRSUniversitéGrenoble Alpes38610 GièresFrance

Under photocatalytic reductive conditions,trifluoromethyl radical addition onto an ynamide followed by cyclization on a benzoyl moiety produces diverse isoindolinone platforms with good *** selectivity of the radical cyclization,N-benzoyl vs.N-benzyl as radical acceptor and the E/Z ratio of isomers have been rationalized by modeling.

关键词： ynamides trifluoromethylation photocatalysis cascade reactions tandem processes isoindolinones modeling

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Entropy Solution to Nonlinear Elliptic Problem with Non-local Boundary Conditions and L^1-data

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Journal of Partial Differential Equations 2017年第3期30卷 201-220页

作者： OUARO Stanislas SOMA Safimba Universite Ouaga I Pr Joseph KI-ZERBO LAboratoire de Mathematique et Informa-tique (LAMI) Unite de Formation et de Recherches en Sciences Exactes et Appliqu~esD~artement de Mathematiques 03 BP 7021 Ouaga 03 Ouagadougou Burkina Faso.

We study a nonlinear elliptic problem with non-local boundary conditions and Ll-data. We prove an existence and uniqueness result of an entropy solution.

关键词： Entropy solution non-local boundary conditions Leray-Lions operator.

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genome-wide differences in hepatitis C-vs alcoholism-associated hepatocellular carcinoma

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World Journal of gastroenterology 2008年第11期14卷 1749-1758页

作者： Céline derambure Cédric Coulouarn Frédérique Caillot Romain Daveau Martine Hiron Michel Scotte Arnaud Franois Celia Duclos Odile goria Marie gueudin Catherine Cavard Benoit Terris Maryvonne Daveau Jean-Philippe Salier Inserm Unité519 and Institut Fédératif de recherches Multidisciplinaires sur les Peptides Facultéde Médecine-PharmacieRouenFrance Inserm Unité519 and Institut Fédératif de recherches Multidisciplinaires sur les Peptides Facultéde Médecine-PharmacieRouenFrance Service de Chirurgie Générale et DigestiveCentre Hospitalier UniversitaireRouenFrance Département de Pathologie Centre Hospitalier UniversitaireRouenFrance Inserm Unité519 and Institut Fédératif de recherches Multidisciplinaires sur les Peptides Facultéde Médecine-PharmacieRouenFrance Service d'Hépato-gastro-enterologieCentre Hospitalier UniversitaireRouenFrance Laboratoire de Virologie and UPres EA 2646 Centre Hospitalier UniversitaireRouenFrance Inserm Unité567 CNRS UMR 8104 UniversitéParis 5Département GDPMInstitut CochinParis France

AIM:To look at a comprehensive picture of etiology- dependent gene abnormalities in hepatocellular carcinoma in Western Europe. MetHODS:With a liver-oriented microarray,transcript levels were compared in nodules and cirrhosis from a training set of patients with hepatocellular carcinoma (alcoholism,12;hepatitis C,10)and 5 *** or tight selection of informative transcripts with an abnormal abundance was statistically valid and the tightly selected transcripts were next quantified by qRTPCR in the nodules from our training set(12+10) and a test set(6+7). resULTS:A selection of 475 transcripts pointed to significant gene over-representation on chromosome 8 (alcoholism)or-2(hepatitis C)and ontology indicated a predominant inflammatory response(alcoholism)or changes in cell cycle regulation,transcription factors and interferon responsiveness(hepatitis C).A stringent selection of 23 transcripts whose differences betweenetiologies were significant in nodules but not in cirrhotic tissue indicated that the above dysregulations take place in tumor but not in the surrounding *** 23 transcripts separated our test set according to etiologies. The inflammation-associated transcripts pointed to limited alterations of free iron metabolism in alcoholic vs hepatitis C tumors. CONCLUSION:etiology-specific abnormalities(chromo- some preference;differences in transcriptomes and related functions)have been identified in hepatocellular carcinoma driven by alcoholism or hepatitis *** may open novel avenues for differential therapies in this disease.

关键词： Alcoholism Chromosome Cirrhosis Hepatitis C Transcriptomes Protein function

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Effects of glutamine supplementation on gut barrier,glutathione content and acute phase response in malnourished rats during inflammatory shock

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World Journal of gastroenterology 2007年第20期13卷 2833-2840页

作者： Liliana Belmonte Mose Co■ffier Florence Le Pessot Olga Miralles-Barrachina Martine Hiron Antony Leplingard Jean-Franois Lemeland Bernadette Hecketsweiler Maryvonne Daveau Philippe Ducrotté Pierre Déchelotte Appareil Digestif Environnement Nutrition ADEN EA3234Institut Hospitalo-Universitaire de Recherche Biomédicale and Institut Fédératif de Recherches Multidisciplinaires sur les PeptidesFaculté de Médecine-PharmacieRouenFrance Service d’Anatomie Pathologique Centre Hospitalier UniversitaireRouenFrance INSERM unit 519 Institut Hospitalo-Universitaire de Recherche Biomédicale and Institut Fédératif de Recherches Multidisciplinaires sur les PeptidesFaculté de Médecine-PharmacieRouenFrance groupe de Recherche sur les Antimicrobiens et les Microorganismes GRAM EA2656Institut Hospitalo-Universitaire de Recherche Biomédicale and Institut Fédératif de Recherches Multidisciplinaires sur les PeptidesFaculté de Médecine-PharmacieRouenFrance

AIM: To evaluate the effect of glutamine on intestinal mucosa integrity,glutathione stores and acute phase response in protein-depleted rats during an inflammatory shock. MetHODS: Plasma acute phase proteins (APP),jejunal APP mRNA levels,liver and jejunal glutathione concentrations were measured before and one,three and seven days after turpentine injection in 4 groups of control,protein-restricted,protein-restricted rats supplemented with glutamine or protein powder. Bacterial translocation in mesenteric lymph nodes and intestinal morphology were also assessed. resULTS: Protein deprivation and turpentine injection significantly reduced jejunal villus height,and crypt depths. Mucosal glutathione concentration significantly decreased in protein-restricted rats. Before turpentine oil,glutamine supplementation restored villus heights and glutathione concentration (3.24 ± 1.05 vs 1.72 ± 0.46 μmol/g tissue,P glutathione remained low. glutamine markedly increased jejunal α1-acid glycoprotein mRNA level after turpentine oil but did not affect its plasma concentration. Bacterial translocation in protein-restricted rats was not prevented by glutamine or protein powder supplementation. CONCLUSION: glutamine restored gut glutathione stores and villus heights in malnourished rats but had no preventive effect on bacterial translocation in our model.

关键词： Acute phase response glutamine glutathione Intestine Malnutrition

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Effectiveness of Four Integrated Pest Management Approaches in the Control of Fruit Flies (Diptera: Tephritidae) in Mango Agro-Ecosystems in the South-Sudanian Zone of Burkina Faso

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Advances in Entomology 2023年第3期11卷 124-142页

作者： Issaka Zida Karim Nébié Alizèta Sawadogo Boureima Tassembédo Timothée Kiénou Rémy A. Dabiré Souleymane Nacro Centre National de la Recherche Scientifique et Technologique Institut de l’Environnement et de Recherches Agricoles Direction Régionale de Recherches Environnementales et Agricoles de l’Ouest Station de Farako-Ba Bobo-Dioulasso Burkina Faso Ecole Nationale de Formation Agricole de Matourkou Bobo-Dioulasso Burkina Faso Centre National de la Recherche Scientifique et Technologique Institut de l’Environnement et de Recherches Agricoles Centre de Recherches Environnementales Agricoles et de Formation Station de Kamboinsé Ouagadougou Burkina Faso

This study evaluated the effectiveness of four Integrated Pest Management (IPM) approaches in the control of Bactrocera dorsalis (Hendel) [Diptera: Tephritidae] and Ceratitis cosyra (Walker) [Diptera: Tephritidae] during two consecutive mango fruiting seasons (2018 and 2019) in the south-Sudanian zone of Burkina Faso. These approaches, including sanitation + M3 bait station (SM), sanitation + protein gF-120 bait (Sg), sanitation + Timaye + M3 bait station (STM) and sanitation + Timaye + gF-120 bait (STg), were implemented in 12 mango orchards in three provinces of the country. In each province, one mango orchard was used as control. Flies per trap per week (FTW) and damage indices were assessed in treated orchards compared to the control orchards. The efficacy rate of each IPM approach in protecting mango against fruit fly attacks was also determined. The STg approach was the most effective in reducing both B. dorsalis and C. cosyra FTW with the best efficacy rate. Further research should emphasize indigenous and affordable attract-and-kill tools for resource poor farmers.

关键词： Bactrocera Ceratitis Control IPM Mango Burkina Faso

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Larvicidal activity of Neem oil and three plant essential oils from Senegal against Chrysodeixis chalcites(Esper, 1789)

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Asian Pacific Journal of Tropical Biomedicine 2018年第1期8卷 67-72页

作者： Saliou Ngom Raimundo Cabrera Perez Ma Anta Mbow Rokhaya Fall Saliou Niassy Andreea Cosoveanu Serigne Mbacké Diop El Hadji Barka Ndiaye Moussoukhoye Diop georges Lognay Institut Sénégalais de recherches Agricoles Unidad de Fitopatologia Facultad de Biología Universidad de la Laguna Département de chimie Faculté des Sciences et Techniques Université Cheikh Anta Diop de Dakar development University of Pretoria gembloux Agro-Bio Tech Université de Liège Chimie Analytique Department Agro Bio Tech

Objective: To evaluate the insecticide, larvicidal and repellent activity of the essential oils from Callistemon viminalis, Melaleuca leucadendron, and Hyptis suaveolens against Chrysodeixis chalcites and to compare it with neem oil(Azadirachta indica). Methods: The essential oils of the leaves of these aromatiques plants were extracted by steam distillation and contacts tests were carried out. results: Essential oils in ethanol from Callistemon viminalis showed a higher biological activity than the neem with 100% larval mortality at the concentration of 2 μg/m L for 6 h, 100% and 90% in ethanol from Melaleuca leucadendron and Hyptis suaveolens, respectively at the concentration of 4 μg/m L for 24 h. By inhalation, the essential oils from Melaleuca leucadendron and of Hyptis suaveolens were more effective with mortality rates of larvae 100% and 50% respectively at 2 μg/L air applied after 24 h. Nevertheless, the neem has shown to be a repulsive plant and anti-nutritional plant. A significant difference in the percentages of consumption between leaves treated with neem oil and the control samples was observed(Newman-Keuls test) except for Melaleuca leucadendron. Conclusions: The results of the study highlight remarkable biocide, properties of tested extracts, which provides important opportunities for the development of biopesticides.

关键词： Plant extracts Chemical constituents Insecticidal activity Chrysodeixis

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Phenolic compounds removal by wet air oxidation based processes

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Frontiers of Environmental Science & Engineering 2018年第1期12卷 5-24页

作者： Linbi Zhou Hongbin Cao Claude descorme Yongbing Xie School of Chemical Engineering ＆ Technology Tianjin University Tianjin 300072 China Division of Environmental Engineering and Technology Institute of Process Engineering Chinese Academy of Sciences Beijing 100190 China Institut de recherches sur la catalyse et l＇environnement de Lyon （IRCELYON） CNRS 2 Avenue Albert Einstein 69626 Villeurbanne Cedex France

Wet air oxidation （WAO） and catalytic wet air oxidation （CWAO） are efficient processes to degrade organic pollutants in water. In this paper, we especially reviewed the WAO and CWAO processes for phenolic compounds degradation. It provides a comprehensive introduction to the CWAO processes that could be beneficial to the scientists entering this field of research. The influence of different reaction parameters, such as temperature, oxygen pressure, pH, stirring speed are analyzed in detail; Homogenous catalysts and heterogeneous catalysts including carbon materials, transitional metal oxides and noble metals are extensively discussed, among which Cu based catalysts and Ru catalysts were shown to be the most active. Three different kinds of the reactor implemented for the CWAO （autoclave, packed bed and membrane reactors） are illustrated and compared. To enhance the degradation efficiency and reduce the cost of the CWAO process, biological degradation can be combined to develop an integrated technology.

关键词： Wet air oxidationCatalytic wet air oxidationPhenolic compoundsHeterogeneous catalystsMechanism

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