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Antineoplastic effects of deoxyelephantopin,a sesquiterpene lactone from Elephantopusscaber, on lung adenocarcinoma (A549) cells

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Journal of Integrative Medicine 2013年第4期11卷 269-277页

作者： Farha A. Kabeer Geetha B. Sreedevi Mangalam S. Nair Dhanya S. Rajalekshmi LathaP. Gopalakrishnan Sujathan Kunjuraman Remani Prathapan Division of Cancer Research Regional Cancer Centre Jawaharlal Nehru Tropical botanic garden and Research Institute National Institute of Interdisciplinary Science and Technology

OBJECTIVE： Deoxyelephantopin, a sesquiterpene lactone from Elephantopus scaber, showed inhibition of the growth of various tumor cells in vitro. In the present study, we investigated the cytotoxicity and apoptosis-inducing capacity of deoxyelephantopin on lung adenocarcinoma （A549） cells. METHODS： The cytotoxic effect of deoxyelephantopin on A549 cells and normal lymphocytes was evaluated using 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide （MTT） assay and 50% inhibitory concentration （IC50） value was determined. The self-renewal and proliferating potential of A549 cells after treatment with deoxyelephantopin were examined by colony formation assay. Cellular morphology of deoxyelephantopin-treated cells was observed using phase- contrast microscopy. The induction of apoptosis was evaluated using acddine orange and ethidium bromide staining, Hoechst 33342 staining, terminal deoxynucleotidyl transferase-mediated dUTP biotin nick end-labeling （TUNEL） assay, DNA fragmentation analysis and Annexin V-fluorescein isothiocyanate staining by flow cytometry. Activation of caspases was detected using fluorogenic substrate specific to caspases 2, 3, 8 and 9 and flow cytometric analysis. The total cellular DNA content and expression of cleaved poly （ADP-ribose） polymerase was also analyzed. RESULTS： Deoxyelephantopin exhibited cytotoxicity to A549 cells （IC50 = 12.287 μg/mL）, however, there was no toxicity towards normal human lymphocytes. Deoxyelephantopin suppressed the colony-forming ability of A549 cells in a dose-dependent manner. Acridine orange, ethidium bromide and Hoechst 33342 staining showed cell shrinkage, chromosomal condensation and nuclear fragmentation, indicating induction of apoptosis. Deoxyelephantopin increased apoptosis of A549 cells, as evidenced by more TUNEL-positive cells. DNA fragmentation and Annexin V staining revealed late-stage apoptotic cell population. Deoxyelephantopin inhibited A549 cell growth by cell cycle arrest at G2/M phase an

关键词： herbal medicine lung neoplasms Elephantopus scaber , deoxyelephantopin apoptosis caspases cell line, tumor A549 cells

Taxonomic history and identity of Musa dasycarpa,*** and *** (Musaceae) in Southeast Asia

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植物分类学报 2008年第2期46卷 230-235页

作者： Markku HKKINEN Henry VRE Xishuangbanna Tropical botanical garden Chinese Academy of Sciences Menglun Mengla Yunnan 666303 China Botanic Garden University of Helsinki P.O. Box 44 (Jyrngntie 2) FI-00014 Finland Finnish Museum of Natural History Botanical Museum University of Helsinki P.O. Box 7 (Unioninkatu 44) FI-00014 Finland

Since the initial description, the name Musa dasycarpa Kurz (1867) has been unclear to most botanists. It has usually been synonymized with M. velutina H. Wendl. & Drude (1875). However, although the original diagnosis was very short, 'fruits hairy', it is adequate. Thus, according to Vienna Codes, M. dasycarpa Kurz has priority over M. velutina H. Wendl. & Drude. The aim of this study is to settle the true identity and to update the description of M. dasycarpa Kurz. For that purpose the names M. dasycarpa and M. velutina are typified. In addi-tion, critical notes regarding M. assamica Bull. are given; it is neotypified here, and considered as conspecific with M. sanguinea Hook. f.

关键词：香蕉科南亚地区分类鉴定野生种质

Male Advertisement Call of the Endangered Leptobrachella tengchongensis(Anura: Megophryidae) from Mount Gaoligongshan, Yunnan Province, China

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Asian Herpetological Research 2021年第2期12卷 221-227页

作者： Ho Yuen YEUNG Xiangyuan HUANG Shenpin YANG Jianhuan YANG Kadcxfrie Conservation China Department Kcuioorie Farm and Botanic GardenLam Kam RoadTai PoN.T.Hong KongChina Gaoligongshan National Nature Reserve(Tengchong Bureau) Tengchong 679100YunnanChina

We present the first description of the acoustic characters of the endangered Tengchong leaf litter toad(Leptobrachella tengchongensis), aiming to inform future ecological studies of the species and taxonomic studies in the genus Leptobrachella. A total of 1179 calls, belonging to 6 adults male individuals, were recorded using TASCAM DR-40 digital sound recorder from its type locality in May 2015 and then analyzed using Raven Pro v.1.6.1 software. The advertisement call of male L. tengchongensis consists of primary advertisement call and secondary advertisement call. The primary advertisement call is invariably composed of two notes and has a call duration of 61 ± 5 ms(23–87 ms, n = 1142). The secondary advertisement call is composed of 3–10 notes(mean 5.25 ± 1.41 notes/call, n = 37) with a call duration of 90 ± 6 ms(37–127 ms, n = 37). The mean inter-call interval is 212 ± 121 ms(88–1121 ms, n = 1092). An introductory note is absent in advertisement calls and the dominant of calls is 4.1–4.8 kHz(at 20 ℃). We discuss the interspecific and intraspecific variations on advertisement calls in L. tengchongensis and among Leptobrachella species, and provide a summary on the major acoustic characters of all Leptobrachella species occurring north of the Isthmus of Kra.

关键词： acoustic analysis amphibians bioacoustics breeding ecology Leptolalax taxonomy Tengchong

A taxonomic revision of Musa aurantiaca (Musaceae) in Southeast Asia

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植物分类学报 2008年第1期46卷 89-92页

作者： Markku HKKINEN Henry VRE Xishuangbanna Tropical botanical garden Chinese Academy of Sciences Menglun Mengla Yunnan 666303 China Botanic Garden University of Helsinki P.O. Box 44 (Jyrngntie 2) FI-00014 Finland Finnish Museum of Natural History Botanical Museum University of Helsinki P.O. Box 7 (Unioninkatu 44) FI-00014 Finland)

Since the initial description, the name Musa aurantiaca Baker (1893) has been unclear to most botanists. The aim of this study is to settle its true identity and to update the description. The plant is distributed in the regions of Upper Assam and Arunachal Pradesh, India, Northern Myanmar and Tibet, China where it occurs commonly but it is not mentioned in Chinese literature at all. In this paper, the authors also review the description and the literature history of M. aurantiaca from 1893 to the present. Musa aurantiaca Baker is typified here.

关键词：香蕉科东南亚分类学野生香蕉芭蕉

Dismantling Melaspileaceae:a first phylogenetic study of Buelliella,Hemigrapha,Karschia,Labrocarpon and Melaspilea

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Fungal Diversity 2015年第2期71卷 141-164页

作者： Damien Ertz Paul Diederich Department Bryophytes-Thallophytes(BT) Botanic Garden MeiseNieuwelaan 38B–1860 MeiseBelgium Musée national d’histoire naturelle 25 rue MunsterL–2160 LuxembourgLuxembourg

Melaspileaceae is a heterogeneous group of Ascomycota including lichenized,lichenicolous and saprobic fungi.A first phylogenetic study of Melaspileaceae is presented and is based on mtSSU and nuLSU sequence *** obtained 49 new sequences for 28 specimens representing 15 *** genera Buelliella,Hemigrapha,Karschia,Labrocarpon and Melaspilea *** included in a molecular phylogeny for the first *** is recovered as polyphyletic,with members placed in two main lineages of *** *** included in *** is placed in synonymy with *** genus Encephalographa is placed in *** genera Buelliella,Karschia,Labrocarpon and several members of Melaspilea are demonstrated to belong to Asterinales,while Hemigrapha is confirmed in this *** genera Melaspileella,Melaspileopsis,Stictographa are reinstated for former Melaspilea species now placed in *** cezannei is described as new,and the new combinations Melaspilea costaricensis,***,***,Melaspileella proximella and Melaspileopsis diplasiospora are *** as newly defined includes lichenized and saprobic *** lichenicolous and saprobic life styles form different intermixed lineages in Asterinales that do not include lichenized *** phylogenetic data provide a first framework for dismantling further the genus Melaspilea for which most of the species are expected to belong to Asterinales.

关键词： Asterinales Eremithallales Lichenicolous fungi Phylogeny Taxonomy

Natural traps of spores and pollen grains from the region surrounding Wenbi Reservoir, Yunnan, China

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科学通报 2013年第S1期58卷 162-168页

作者： SONG XiaoYan BERA Subir YAO YiFeng BLACKMORE Stephen LI ChengSen State Key Laboratory of Systematic and Evolutionary Botany Institute of BotanyChinese Academy of Sciences Shanxi Agricultural University Department of Botany University of Calcutta Royal botanic garden Edinburgh

We did a comparative analysis of pollen and spores from five kinds of natural pollen traps at Wenbi Reservoir, northwest Yunnan, China. The natural traps were the surface soil, ground inhabiting mosses, spider webs, tree bark and moss/lichen on trees. These traps showed qualitative and quantitative variations in the pollen and spore spectra. The palynomorphs recovered from the soil samples of trees and shrubs ranged from 90.54% to 95.98% of the total counts. These counts were higher than counts from ground mosses(53.13%–81.66%), spider webs(61.71%), bark samples(53.8%) and moss/lichen on trees(50%–53.6%). The result of pollen/spore analysis showed that surface soil, ground mosses and spider webs better reflect the local vegetation compared with tree bark or moss/lichen on trees.

关键词： natural pollen traps Wenbi Reservoir Yunnan China vegetation

Plant species with extremely small populations(PSESP) in China:A seed and spore biology perspective

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Plant Diversity 2016年第5期38卷 209-220页

作者： Ellie Merrett Wade Jayanthi Nadarajan Xiangyun Yang Daniel Ballesteros Weibang Sun Hugh W.Pritchard Royal botanic gardens KewWellcome Trust Millennium Building The Germplasm Bank of Wild Species Kunming Institute of BotanyChinese Academy of Sciences Kunming botanical garden Kunming Institute of BotanyChinese Academy of Sciences

Approximately one fifth of the world's plants are at risk of *** these,a significant number exist as populations of few individuals,with limited distribution ranges and under enormous pressure due to habitat *** China,these most-at-risk species are described as 'plant species with extremely small populations'(PSESP).Implementing conservation action for such listed species is *** seeds is one of the main means of ex situ conservation for flowering *** storage could provide a simple and economical method for fern ex situ *** and spore germination in nature is a critical step in species regeneration and thus in situ *** what is known about the seed and spore biology(storage and germination) of at-risk species? We have used China's PSESP(the first group listing) as a case study to understand the gaps in knowledge on propagule biology of threatened plant *** found that whilst germination information is available for 28 species(23%of PSESP),storage characteristics are only known for 8%of PSESP(10 species).Moreover,we estimate that 60%of the listed species may require cryopreservation for long-term *** conclude that comparative biology studies are urgently needed on the world's most threatened taxa so that conservation action can progress beyond species listing.

关键词： Threatened species Orchids Storage characteristics Cryopreservation ex situ conservation

Taxonomic synopsis of Berberis(Berberidaceae)from the northern Hengduan mountains region in China,with descriptions of seven new species

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Plant Diversity 2022年第5期44卷 505-517页

作者： Yao-Ke Li Julian Harber Chuan Peng Zhi-Qiang Du Yao-Wu Xing Chih-Chieh Yu CAS Key Laboratory of Tropical Forest Ecology Xishuangbanna Tropical Botanical GardenChinese Academy of SciencesMenglaYunnan666303China University of Chinese Academy of Sciences Beijing100049China Research Associate Missouri Botanic GardenMytholmroydWest YorkshireUK Center of Plant Ecology Core Botanical GardensChinese Academy of SciencesMenglaYunnan666303China

Though Berberis(Berberidaceae)is widely distributed across the Eurasian landmass it is most diverse in the Himalaya-Hengduan Mountain(HHM)*** are more than 200 species in China where it is one of the most common mountain *** study on the taxonomy and evolution of Berberis in this region can thus provide an important insight into the origin and diversification of its flora.A prerequisite to this is mapping and describing the various species of Berberis in the region-a task that despite recent progress is by no means *** is clear that in China there may be a significant number of species still to be described and that even with published species much about their distribution remains to be *** a contribution to the first of these tasks seven new species from the northern Hengduan Mountain of *** and ***:Berberis chinduensis,Berberis degexianensis,Berberis jiajinshanensis,Berberis jinwu,Berberis litangensis,Berberis longquensis and Berberis riparia,are described *** in overall morphology and especially in floral structures with each other and with similar species of Berberis in the same region are *** report is the result of phylogenetic analyses based on plastome and partial nrDNA sequences of both the seven proposed new species and a significant number of similar species already *** conclusions as to the insights provides on the history of the genetic divergence are discussed.

关键词： Berberidaceae Flora of China Herbarium taxonomy Plastome sequences nrDNA sequences

Nutritional Aspects of Two Widely Consumed Wild Edible Mushrooms in Jordan

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Journal of Life Sciences 2013年第6期7卷 653-657页

作者： Ahmad Mohamad Almomany Royal botanic garden Amman-Tell ar Rumman 11910 Jordan

Two wild mushrooms frequently consumed in Jordan were collected form Jordan forests in spring 2012. Each sample was studied in the lab by taking measurements on the morphological structures of the cap, stem, and recording gill＇s color, shape and dimensions. Also smell and taste for each fruiting structure were taken in consideration. Spore print for each sample was also defined. Spores of each mushroom were microscopically tested and measured. These mushrooms were identified as Pleurotus eryngi and Tricholoma terreum. The two mushrooms were evaluated for their physical properties, chemical and mineral composition. Contents of Ca, Na, K, Zn, Mn, Cu, Fe and Mg were determined. For each mushroom species two mixed samples were analyzed. Nitrogen content was detected with Kjeldahl method and protein content （%） was calculated by multiplying nitrogen content with the factor 4.38. The highest protein content calculated on oven dry weight basis was found in Pleurotus eryngi which was around 30.6%, while Tricholoma terreum contained lowest protein content （17.4%）. Pleurotus eryngi accumulated trace elements at high ratios as for P, Ca and Na 0.99%, 0.65% and 0.57% respectively while Tricholoma terreum contained more K （5.1%） than the other wild mushroom. Total soluble carbohydrates （50.8%）, crude fiber （13.4%） and ash （12.2%） percentages were higher in Tricholoma terreum than in Pleurotus eryngi.

关键词： Mushroom Pleurotus Tricholoma protein fat ash minerals.