High universality of matK primers for barcoding gymnosperms

作     者：Ram C.POUDEL Alan FORREST 

作者机构：Key Laboratory of Biodiversity and BiogeographyKunming Institute of BotanyChinese Academy of Sciences Germplasm Bank of Wild Species in Southwest ChinaKunming Institute of BotanyChinese Academy of Sciences The Graduate University of Chinese Academy of Sciences Royal Botanic Garden Edinburgh 

出 版 物：《Journal of Systematics and Evolution》 (植物分类学报(英文版))

年 卷 期：2011年第49卷第3期

页      面：169-175页

核心收录：

学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学] 

基　　金：supported by the Research Fund for the Large-Scale Scientific Facilities of Chinese Academy of Sciences(Grant No. 2009-LSF-GBOWS-01) the West Light Programme of the Chinese Academy of Sciences (Grant No. 9223111W1) the Talent Project of YunnanProvince,China (Grant No. 2008PY064) grateful to the Gordon and Betty Moore Foundation (#2535) for funding which supported the development of matK primers described in this paper 

主　　题：DNA barcoding matK gymnosperm primer universality. 

摘      要：DNA barcoding is a tool to provide rapid and accurate taxonomic identification using a standard DNA region.A two-marker combination of matK+rbcL was formally proposed as the core barcode for land plants by the Consortium for the Barcode of Life Plant Working ***,there are currently no barcoding primers for matK showing high universality in *** used 57 gymnosperm species representing 40 genera,11 families and four subclasses to evaluate the universality of nine candidate matK primers and one rbcL primer in this ***(1F/724R) of rbcL is proposed here as a universal primer for gymnosperms due to high *** of the nine candidate matK primers(GymF1A/GymR1A) is proposed as the best universal matK primer for gymnosperms because of high polymerase chain reaction success and routine generation of high quality bidirectional sequences.A specific matK primer for Ephedra was newly designed in this study,which performed well on the sampled *** primers proposed here for rbcL and matK can be easily and successfully amplified for most gymnosperms.