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Mannanase improves the growth performance of broilers by alleviating inflammation of the intestinal epithelium and improving intestinal microbiota

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Animal Nutrition 2024年第1期16卷 376-394页

作者： Xiaodan Zhang Huiping Xu Lu Gong Jiao Wang Jianyang Fu Zengpeng Lv Liangjuan Zhou Xuejun Li Qiong Liu Pingyu Xia Yuming Guo State Key Laboratory of Animal Nutrition and Feeding College of Animal Science and Technology China Agricultural University Beijing Strowin biotechnology Co. Ltd.

This experiment aimed to discuss and reveal the effect and mechanism of mannanase on intestinal inflammation in broilers triggered by a soybean meal diet. In this experiment, 384 Arbor Acres broilers at1 d old were randomly divided into 3 treatment groups. The broilers were fed a corn-soybean meal basal diet, a low-energy diet(metabolizable energy reduced by 50 kcal/kg), and a low-energy diet supplemented with 100 mg/kg mannanase for 42 d. The low-energy diet increased feed conversion ratio from0 to 42 d, reduced ileal villus height and villus height-to-crypt depth ratio and upregulated the expression of nuclear factor kappa B(NF-κB) in the ileum(P < 0.05). It also reduced cecal short-chain fatty acids(SCFA), such as acetic acid(P < 0.05). Compared with low-energy diets, the addition of mannanase increased body weight at 42 d, promoted the digestibility of nutrients, and maintained the morphology and integrity of the intestinal epithelium of broilers(P < 0.05). In addition, mannanase upregulated the expression of claudin-1(CLDN1) and zonula occludens-1(ZO-1) in the jejunum at 21 d,downregulated the expression of ileal NF-κB, and increased the content of isobutyric acid in the cecum of broilers(P < 0.05). The results for the ileal microbiota showed that a low-energy diet led to a decrease in the relative abundance of Lactobacillus reuteri in the ileum of broilers. The addition of mannanase increased the relative abundance of Lactobacillus-KC45b and Lactobacillus johnsonii in broilers. Furthermore, a low-energy diet reduced the relative abundance of Butyricicoccus in the intestine of broilers and inhibited oxidative phosphorylation and phosphoinositol metabolism. Mannanase increased the relative abundance of Odoribacter, promoted energy metabolism and N-glycan biosynthesis, and increased the activities of GH3 and GH18. It is concluded that mannanase could improve the growth performance of broilers by reducing the expression of NF-κB in the ileum, increasing the produc

关键词： Broiler Low-energy diet Mannanase Intestinal inflammatory response Intestinal microbiota Metagenome

Reorganization of 3D genome architecture provides insights into pathogenesis of early fatty liver disease in laying hens

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Journal of Animal science and biotechnology 2024年第3期15卷 1086-1100页

作者： Yanli Liu Zhuqing Zheng Chaohui Wang Yumeng Wang Xi Sun Zhouzheng Ren Xin Yang Xiaojun Yang college of Animal science and Technology Northwest A&F UniversityYangling 712100China Institute of Agricultural biotechnology Jingchu University of TechnologyJingmen 448000China

Background Fatty liver disease causes huge economic losses in the poultry industry due to its high occurrence and lethality ***-dimensional(3D)chromatin architecture takes part in disease processing by regulating tran-scriptional *** study is carried out to investigate the alterations of hepatic 3D genome and H3K27ac profiling in early fatty liver(FLS)and reveal their effect on hepatic transcriptional reprogramming in laying *** Results show that FLS model is constructed with obvious phenotypes including hepatic visible lipid deposi-tion as well as higher total triglyceride and cholesterol in serum.A/B compartment switching,topologically associat-ing domain(TAD)and chromatin loop changes are identified by high-throughput/resolution chromosome conforma-tion capture(HiC)*** genes of these alternations in hepatic 3D genome organization significantly enrich pathways related to lipid metabolism and hepatic damage.H3K27ac differential peaks and differential expres-sion genes(DEGs)identified through RNA-seq analysis are also enriched in these ***,certain DEGs are found to correspond with changes in 3D chromatin structure and H3K27ac binding in their *** motif analysis reveals that candidate transcription factors are implicated in regulating transcriptional ***,disturbed folate metabolism is observed,as evidenced by lower folate levels and altered enzyme *** Our findings establish a link between transcriptional reprogramming changes and 3D chromatin struc-ture variations during early FLS formation,which provides candidate transcription factors and folate as targets for FLS prevention or treatment.

关键词： 3D chromatin architecture Fatty liver disease Folate H3K27ac profiling Transcription reprogramming

Comparative metabolomics reveal histamine biodegradation mechanism by salt stressed Bacillus subtilis JZXJ-7

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food science and Human Wellness 2024年

作者： Rundong Wang Yijia Deng Yuhao Zhang Xuepeng Li Ravi Gooneratne Jianrong Li college of food science and Engineering Bohai University Chongqing Key Laboratory of Speciality food Co-Built by Sichuan and Chongqing Department of Wine food and Molecular Biosciences Faculty of Agriculture and Life sciences Lincoln University Lincoln 7647 New Zealand college of food science Southwest University college of food science and Engineering Lingnan Normal University

Bacillus subtilis (B. subtilis) JZXJ-7 isolated from shrimp paste can significantly degrade histamine under salt stress but the mechanism is unclear. This study aims to evaluate the effect of 170 and 340 mmol/L NaCl on B. subtilis JZXJ-7 growth， histamine degradation， antioxidant enzymes [catalase (CAT)， superoxide dismutase (SOD) and glutathione S-transferase (GST)] activities and Na+， K+-ATPase activity. Furthermore， comparative metabolomics was used to investigate histamine biodegradation mechanism by *** JZXJ-7 subjected to salt stress. Both 170 and 340 mmol/L NaCl promoted B. subtilis JZXJ-7 growth in late stages of reproduction (32 - 48 h)， increased histamine degradation rate by 64.85% and 79.87% (P food fermentation industry.

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Understanding the mechanism underlying the anti-inflammatory effect of dietary flavonoids on IBD: a focus on gut microbiota and its metabolites involved in SCFA， bile acid， tryptophan and lipopolysaccharides

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food science and Human Wellness 2024年

作者： Huihui Xiong Hui Chen Lingli Chen Kehui Ouyang Suyun Lin Wenjun Wang college of food science and Engineering Jiangxi Agricultural University college of Animal science and Technology Jiangxi Agricultural University

Inflammatory bowel disease (IBD) is a chronic gastrointestinal disease with a high incidence. Treatment for IBD includes medications and diet， and common anti-inflammatory medications have limitations like drug resistance and serious adverse effects. Accumulating evidence has demonstrated that dietary flavonoids exhibit an alleviative effect on IBD by influencing gut microbiota. The microbiota-derived metabolites also regulate IBD and maintain intestinal homeostasis. In this review， we investigate the therapeutic effect of gut microbiota and metabolites on IBD by intestinal immune and intestinal barrier function. We demonstrate the underlying mechanism of dietary flavonoids as an anti-inflammatory molecule alleviating IBD by regulating gut microbiota， short chain fatty acid (SCFA)， bile acid (BA)， tryptophan (Trp) metabolism and lipopolysaccharides (LPS)-toll-like receptor 4 (TLR4) signaling pathway. Based on structural differences of flavonoids， we summarize the recent research progress on the role of different dietary flavonoids in alleviating IBD by gut microbiota and metabolites in animal and clinical trials. This review indicates that dietary flavonoids targeting gut microbiota and metabolites provide a promising strategy for the treatment of inflammation and novel insights into the management of IBD.

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High glucose reduces Nrf2-dependent cRAGE release and enhances inflammasome-dependent IL-1βproduction in monocytes:the modulatory effects of EGCG

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food science and Human Wellness 2024年第3期13卷 1531-1542页

作者： Chi-Hao Wu Yin-Hsuan Chang Chin-Lin Hsu Sheng-Yi Chen Gow-Chin Yen Graduate Programs of Nutrition science School of Life ScienceTaiwan Normal UniversityTaipei 106209TaiwanChina Department of food science and biotechnology Chung Hsing UniversityTaichung 40227TaiwanChina School of Nutrition Chung Shan Medical UniversityTaichung 40201TaiwanChina

Soluble receptor for advanced glycation end products(sRAGE)acts as a decoy sequestering of RAGE ligands,thus preventing the activation of the ligand-RAGE axis linking human ***,the molecular mechanisms underlying sRAGE remain *** this study,THP-1 monocytes were cultured in normal glucose(NG,5.5 mmol/L)and high glucose(HG,15 mmol/L)to investigate the effects of diabetesrelevant glucose concentrations on sRAGE and interleukin-1β(IL-1β)*** modulatory effects of epigallocatechin gallate(EGCG)in response to HG challenge were also *** enhanced intracellular reactive oxygen species(ROS)generation and RAGE *** secretion of sRAGE,including esRAGE and cRAGE,was reduced under HG conditions,together with the downregulation of a disintegrin and metallopeptidase 10(ADAM10)and nuclear factor erythroid 2-related factor 2(Nrf2)nuclear ***,the HG effects were counteracted by siRAGE and exacerbated by *** immunoprecipitation results showed that Nrf2 binding to the ADAM10 promoter and HG interfered with this *** data reinforce the notion that RAGE and Nrf2 might be sRAGE-regulating *** HG conditions,the treatment of EGCG reduced ROS generation and RAGE ***-stimulated cRAGE release was likely caused by the upregulation of the Nrf2-ADAM10 *** inhibited HG-mediated NLRP3 inflammasome activation at least partly by stimulating sRAGE,thereby reducing IL-1βrelease.

关键词： Epigallocatechin gallate(EGCG) Inflammasome Nuclear factor erythroid 2-related factor 2(Nrf2) Receptor for advanced glycation end products(RAGE) Soluble RAGE(sRAGE)

Novel silver nanoparticles loaded Cu-based metal organic framework as a promising antimicrobial material for controlling Escherichia coli O157:H7 in pork

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food science and Human Wellness 2024年

作者： Dongbo Yang Jing Yi Xiaoya Li Shuqi Zhang Yiming Xiang Xingxing Liu Zhongguo Shan Haihua Wang college of food science and Engineering Northwest A&F University

The risk of infection following consumption of foodborne pathogens contaminated foods became a significant concern for human health and imposes great economic losses to food industry. Herein， Ag nanoparticles were integrated to Cu-based metal–organic framework (Cu-MOF) for antibacterial activity. The crystal structure， morphology and composition of the prepared composite Ag@Cu-MOF were confirmed by XRD， TG， SEM， TEM， FTIR and XPS analysis. Antibacterial assays revealed that Ag@Cu-MOF exhibited increased inhibitory activity against Escherichia coli O157:H7 in comparison to Cu-MOF. Ag@Cu-MOF treated bacterial cells displayed distinct morphological changes， a decreased ratio of Live/Dead cells， as well as a reduction of intracellular adenosine 5’-triphosphate (ATP). Antibiofilm studies demonstrated that Ag@Cu-MOF could dramatically inhibit biofilm formation and disrupt preformed biofilms by interfering the metabolic activity and decreasing the expression of biofilm-associated genes. food contamination model illustrated that Ag@Cu-MOF significantly prevented the growth of E. coli O157:H7 in packed pork. This study sheds light on the potential of Ag@Cu-MOF as a promising antimicrobial material for preserving pork.

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Untargeted metabolomics combined with 16S rRNA sequencing reveals the characteristics of intestinal metabolism and gut microbiota in hazelnut Cor a 14-allergic BALB/c mice

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food science and Human Wellness 2024年

作者： Chen Chen Kaiyu Mu Qiang Xie Wentong Xue college of food science and Nutritional Engineering China Agricultural University

Cor a 14 is one of the most vital allergens in hazelnuts. However， the features of intestinal metabolites and microbiota associated with Cor a 14-induced allergy remain unclear. In this study， we established a hazelnut Cor a 14-allergic BALB/c mice model， which was distinguished by the dropped temperature and enhanced allergic inflammatory factor levels in serum. Faeces were collected to detect characteristics of the intestinal metabolites by untargeted metabolomics and the gut microbiota by 16S rRNA sequencing. The α- and β-diversity of gut microbiota in Cor a 14-allergic mice differed from the controls with elevated relative abundance of Lactobacillus and reduced relative abundances of Odoribacter， Chloroplast， Alistipes and Lachnospiraceae＿NK4A136＿group. Untargeted metabolomics results revealed that 238 significantly differential metabolites (111 up-regulated， 127 down-regulated) were identified， of which， L-tryptophan， histamine and N-acetylhistamine were remarkably accumulated and primarily enriched in the enhanced L-tryptophan and histidine metabolism pathways. Spearman correlation analysis suggested that L-tryptophan was positively correlated with allergic indicators and screened as the potential biomarker for Cor a 14 allergy. Collectively， our findings uncovered the characteristics of intestinal metabolites and gut microbiota during Cor a 14 anaphylaxis and provided new potential insights for diagnosis of hazelnut allergy.

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Elaidic acid-induced intestinal barrier damage led to gut-liver axis derangement and triggered NLRP3 inflammasome in the liver of SD rats

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food science and Human Wellness 2024年第3期13卷 1279-1291页

作者： Hui Liu Xuenan Li Lu Li Yucai Li Haiyang Yan Yong Pang Wenliang Li Yuan Yuan college of food science and Engineering Jilin UniversityChangchun 130062China School of Public Health Jining Medical UniversityJining 272067China

Previous studies have shown that trans fatty acids(TFA) are associated with several chronic diseases,the gut microbiota is directly influenced by dietary components and linked to chronic *** research investigated the effects of elaidic acid(EA),a typical TFA,on the gut microbiota to understand the underlying mechanisms of TFA-related chronic diseases.16S rDNA gene sequencing on faecal samples from Sprague-Dawley rats were performed to explore the composition change of the gut microbiota by EA gavage for 4 *** results showed that the intake of EA increased the abundance of well-documented harmful bacteria,such as Proteobacteria,Anaerotruncus,Oscillibacter and ***,EA induced translocation of lipopolysaccharides(LPS) and the above pathogenic bacteria,disrupted the intestinal barrier,led to gut-liver axis derangement and TLR4 pathway activation in the ***,EA induced intestinal barrier damage and regulated TLR4-MyD88-NF-κB/MAPK pathways in the liver of SD rats,leading to the activation of NLRP3 inflammasome and inflammatory liver damage.

关键词： Elaidic acid(EA) Gut microbiota Intestinal barrier Gut-liver axis TLR4-MyD88-NF-κB/MAPK pathways NLRP3 inflammasome

Evaluation of the intracellular lipid-lowering effect of polyphenols extract from highland barley in HepG2 cells

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food science and Human Wellness 2024年第1期13卷 454-461页

作者： Yijun Yao Zhifang Li Bowen Qin Xingrong Ju Lifeng Wang college of food science and Engineering/Collaborative Innovation Centre for Modern Grain Circulation and Safety Nanjing University of Finance and EconomicsNanjing 210023China

Active ingredients from highland barley have received considerable attention as natural products for developing treatments and dietary supplements against *** practical application,the research of food combinations is more significant than a specific food *** study investigated the lipid-lowering effect of highland barley polyphenols via lipase assay in vitro and HepG2 cells induced by oleic acid(OA).Five indexes,triglyceride(TG),total cholesterol(T-CHO),low density lipoprotein-cholesterol(LDL-C),aspartate aminotransferase(AST),and alanine aminotransferase(ALT),were used to evaluate the lipidlowering effect of highland barley *** also preliminary studied the lipid-lowering mechanism by Realtime fluorescent quantitative polymerase chain reaction(q PCR).The results indicated that highland barley extract contains many components with lipid-lowering effects,such as hyperoside and *** vitro,the lipase assay showed an 18.4%lipase inhibition rate when the additive contents of highland barley extract were 100μg/m *** intracellular lipid-lowering effect of highland barley extract was examined using 0.25 mmol/L OA-induced HepG2 *** results showed that intracellular TG,LDL-C,and T-CHO content decreased by 34.4%,51.2%,and 18.4%,*** and AST decreased by 51.6%and 20.7%compared with the untreated hyperlipidemic HepG2 cells.q PCR results showed that highland barley polyphenols could up-regulation the expression of lipid metabolism-related genes such as PPARγand Fabp4.

关键词： Highland barley Polyphenols extract Lipid-lowering effect HepG2 cells