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Reassessing the AMPK-MTORC1 balance in autophagy in the central nervous system

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Neural Regeneration Research 2025年第11期20卷 3209-3210页

作者： Marta García-Juan Mario Villa Irene Benito-Cuesta Lara Ordónez-Gutiérrez Francisco Wandosell Centro de biología molecular“Severo Ochoa”(CSIC-UAM) Nicolas Cabrera 1Universidad Autónoma de MadridMadridSpain Fisiología Animal Departamento de BiologíaFacultad de CienciasUniversidad Autónoma de MadridMadridSpain Department of Clinical Neuroscience CMM Karolinska Universitetssjukhuset SolnaStockholmSweden Departamento de bioquímica y biología molecular Universidad Complutense de MadridMadridSpain Centro de Investigación Biomédica en Red de Enfermedades Neurodegenerativas(CIBERNED) MadridSpain

Autophagy is a cellular degradation and recycling system,indispensable for cellular and organ development,homeostasis,and *** cellular process is evolutionarily highly conserved to quality control of many proteins and dysfunctional organelles,which finally recycle components as amino *** process is effective during normal physiology as part of anabolism and plays an additional important role during starvation(Dikic and Elazar,2018).Different types of autophagy have been chara cterized based on their dynamic,mechanism of action,target substrates,and protein *** of them are macroautophagy(hereafter called"autophagy"),microa utophagy,and chape rone-media ted autophagy(Fleming et al.,2022).

关键词： homeostasis system hereafter

Beyond neurodegeneration:engineering amyloids for biocatalysis

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Neural Regeneration Research 2025年第10期20卷 2915-2916页

作者： Andrea Bartolomé-Nafría Javier García-Pardo Salvador Ventura Institut de Biotecnologia i de Biomedicina(IBB)and Departament de bioquímica i biologia molecular Universitat Autònoma de BarcelonaBellaterraBarcelonaSpain Hospital Universitari Parc Taulí Institut d’Investigaciói InnovacióParc Taulí(I3PT-CERCA)Universitat Autònoma de BarcelonaSabadellSpain

Amyloid fibrils are highly organized protein or peptide aggregates,often characterized by a distinctive supramolecular cross-β-sheet *** formation and accumulation of these structures have been traditionally associated with neural or systemic human diseases,such as Alzheimer’s disease,Parkinson’s disease,type-2 diabetes,or amyotrophic lateral sclerosis(Wei et al.,2017;Wittung-Stafshede,2023).

关键词： structure amyloid

molecular aspects of intestinal calcium absorption

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World Journal of Gastroenterology 2015年第23期21卷 7142-7154页

作者： Gabriela Diaz de Barboza Solange Guizzardi Nori Tolosa de Talamoni Cátedra de bioquímica y biología molecular Facultad de Ciencias Médicas Universidad Nacional de Córdoba

Intestinal Ca2+ absorption is a crucial physiological process for maintaining bone mineralization and Ca2+ homeostasis. It occurs through the transcellular and paracellular pathways. The first route comprises 3steps: the entrance of Ca2+ across the brush border membranes(BBM) of enterocytes through epithelial Ca2+ channels TRPV6, TRPV5, and Cav1.3; Ca2+ movement from the BBM to the basolateral membranes by binding proteins with high Ca2+ affinity(such as CB9k); and Ca2+ extrusion into the blood. Plasma membrane Ca2+ ATPase(PMCA1b) and sodium calcium exchanger(NCX1) are mainly involved in the exit of Ca2+ from enterocytes. A novel molecule, the 4.1R protein, seems to be a partner of PMCA1 b, since both molecules colocalize and interact. The paracellular pathway consists of Ca2+ transport through transmembrane proteins of tight junction structures, such as claudins 2, 12, and 15. There is evidence of crosstalk between the transcellular and paracellular pathways in intestinal Ca2+ transport. When intestinal oxidative stress is triggered, there is a decrease in the expression of several molecules of both pathways that inhibit intestinal Ca2+ absorption. Normalization of redox status in the intestine with drugs such as quercetin, ursodeoxycholic acid, or melatonin return intestinal Ca2+ transport to control values. Calcitriol [1,25(OH)2D3] is the major controlling hormone of intestinal Ca2+ transport. It increases the gene and protein expression of most of the molecules involved in both pathways. PTH, thyroid hormones, estrogens, p ro l a c t i n, g ro w t h h o r m o n e, a n d g l u c o c o r t i c o i d s apparently also regulate Ca2+ transport by direct action, indirect mechanism mediated by the increase of renal 1,25(OH)2D3 production, or both. Different physiological conditions, such as growth, pregnancy, lactation, and aging, adjust intestinal Ca2+ absorption according to Ca2+ demands. Better knowledge of the molecular details of intestinal Ca2+ absorption could l

关键词： Intestinal Ca2+ absorption Transcellularpathway Paracellular route 1,25(OH)2D3 PTH Prolactin Estrogen Lactation Pregnancy Aging

Proteomics for discovery of candidate colorectal cancer biomarkers

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World Journal of Gastroenterology 2014年第14期20卷 3804-3824页

作者： Paula lvarez-Chaver Olalla Otero-Estévez María Páez de la Cadena Francisco J Rodríguez-Berrocal Vicenta S Martínez-Zorzano Unidad de Proteómica Servicio de Determinación EstructuralProteómica y GenómicaCentro de Apoyo Científico y Tecnológico a la InvestigaciónUniversidad de VigoCampus As Lagoas-Marcosende área de bioquímica y biología molecular Departamento de BioquímicaGenética e InmunologíaFacultad de BiologíaUniversidad de VigoCampus As Lagoas-Marcosende

Colorectal cancer(CRC)is the second most common cause of cancer-related deaths in Europe and other Western countries,mainly due to the lack of wellvalidated clinically useful biomarkers with enough sensitivity and specificity to detect this disease at early *** it is well known that the pathogenesis of CRC is a progressive accumulation of mutations in multiple genes,much less is known at the proteome ***,in the last years many proteomic studies have been conducted to find new candidate protein biomarkers for diagnosis,prognosis and as therapeutic targets for this malignancy,as well as to elucidate the molecular mechanisms of colorectal *** important advantage of the proteomic approaches is the capacity to look for multiple differentially expressed proteins in a single *** review provides an overview of the recent reports describing the different proteomic tools used for the discovery of new protein markers for CRC such as two-dimensional electrophoresis methods,quantitative mass spectrometry-based techniques or protein ***,we will also focus on the diverse biological samples used for CRC biomarker discovery such as tissue,serum and faeces,besides cell lines and murine models,discussing their advantages and disadvantages,and summarize the most frequently identified candidate CRC markers.

关键词： Colorectal cancer Serum Tissue Proteins Biomarkers Proteomics Protein identification Mass spectrometry

Different Thermostability of Skeletal Muscle Glyceraldehyde-3-phosphate Dehydrogenase from Hibernating and Euthermic Jerboa (Jaculus orientalis)

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Acta Biochimica et Biophysica Sinica 2003年第10期35卷 891-896页

作者： IDDAR Abdelghani,CAMPOS Luis A.,SANCHO Javier,SERRANO Aurelio,SOUKRI Abdelaziz Laboratoire de Biochimie Departamento de bioquímica y biología molecular y Celular Departamento de bioquímica y biología molecular y Celular Instituto de bioquímica Vegetal y Fotosíntesis Laboratoire de Biochimie Unitè de Gènie Enzymatique et biologie Molèculaire Facultè des Sciences-Ain Chock Casablanca Morocco Universidad de Zaragoza Zaragoza Spain Universidad de Zaragoza Zaragoza Spain CSIC-Universidad de Sevilla Sevilla Spain Unitè de Gènie Enzymatique et biologie Molèculaire Facultè des Sciences-Ain Chock Casablanca Morocco

In previous study, we demonstrated that the specific activity of D-glyceraldehyde-3-phosphate dehydrogenase (GAPDH, EC 1.***.1.12) in skeletal muscle of induced hibernating jerboa (hibernating GAPDH) was 3-4 folds lower than that of the one in the skeletal muscle of the euthermic jerboa (euthermic GAPDH). A significant decrease in both GAPDH protein and GapC mRNA levels occurs when hibernating, but the purified hibernating GAPDH is less active than the euthermic GAPDH. To investigate the physico-chemical basis of this lower activity, the behaviour during thermal inactivation of skeletal muscle GAPDH from hibernating and euthermic tissues was examined by a variety of spectroscopic techniques, including fluorescence emission, circular dichroism and ultraviolet absorption. A clear resistance to thermal denaturation was observed in the hibernating GAPDH compared with the euthermic GAPDH. The different temperature of denaturation found in these proteins by both fluorimetry and circular dichroism indicates that there might exist conformational changes of GAPDH upon hibernation that could affect the stability of this enzyme.

关键词： glyceraldehyde-3-phosphate dehydrogenase hibernation thermal denaturation circular dichroism spectroscopy protein stability

TET2, an “ambiguous” player in inflammation

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Neural Regeneration Research 2020年第8期15卷 1481-1482页

作者： Ana María Espinosa-Oliva Miguel Angel Burguillos Departamento de bioquímica y biología molecular Facultad de Farmacia Universidad de Sevilla and Instituto de Biomedicina de Sevilla (IBiS) Hospital Universitario Virgen del Rocío/CSIC

Microglial cells,the“macrophages”from the central nervous system(CNS),perform a variety of roles necessary to keep the homeostasis in the healthy ***,microglial cells are best known for their role as“first responders”through initiation of an innate immune response against a wide variety of deleterious stimuli in the *** controlled inflammatory response is beneficial and disappears once the deleterious stimuli are gone.

关键词： inflammation homeostasis delete

Oxidative effects of glyphosate on the lipophobic intracellular environment in the microalgae Chlorella vulgaris

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BIOCELL 2022年第3期46卷 795-802页

作者： JUAN MANUEL OSTERA SUSANA PUNTARULO GABRIELA MALANGA Fisicoquímica Facultad de Farmacia y BioquímicaUniversidad de Buenos AiresBuenos AiresCP 1113Argentina Instituto de bioquímica y Medicina molecular(IBIMOL) CONICET-Universidad de Buenos AiresBuenos AiresCP 1113Argentina

The studied hypothesis is that the herbicide glyphosate(GLy)can affect the oxidative balance in the hydrophobic intracellular medium in non-target Chlorella vulgaris *** GLy and RoundUp(RUP)supplementation,affected the growth profile.A significant 42%decrease in the cellular biomass in stationary(St)phase was observed in cultures supplemented with either 5μM of GLy or RUP,as compared to control *** treatment with 0.3μM of GLy generated non-significant effects on the oxidation rate of 2’,7’dichlorofluorescein diacetate(DCFH-DA),neither in exponential(Exp)nor in St phase of development,as compared to control ***,the treatment with either 5μM GLy or 0.3 and 5μM RUP lead to a significant decrease in the DCFH-DA oxidation rate,as compared to control *** lipid radical(LR●)generation rate,detected by Paramagnetic Resonance Spectroscopy(EPR),was significantly increased in the presence of RUP,in Lag and Exp phase of *** non-enzymatic antioxidants,α-Tocopherol(α-T)andβ-Carotene(β-C),are aimed to protect membranes against the damage produced by the radical *** content ofβ-C was not significantly affected,as compared to control cultures,by any of the treatments,in both growth phases of cellular *** content ofα-T was significantly decreased by the supplementation with either 0.3 or 5μM of RUP or 5μM *** LR●/α-T ratio,used as indicator of the oxidative balance in the hydrophobic cellular media,was significantly different between samples obtained from control and RUP-exposed microalgae in both,Exp and St phase of development,with either 0.3 or 5μM *** data presented here showed evidence that suggested that oxidative balance in the hydrophobic environment was affected by either GLy or RUP.

关键词： 2’,7’dichlorofluorescein diacetate oxidation rate Lipid soluble antioxidants Round up Redox balance

Oxidative stress in the hydrophilic medium of algae and invertebrates

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BIOCELL 2016年第1期40卷 35-38页

作者： GABRIELA MALANGA PAULA MARIELA GONZALEZ JUAN MANUEL OSTERA SUSANA PUNTARULO Instituto de bioquímica y Medicina molecular(IBIMOL)-Universidad de Buenos Aires(UBA) CONICETFisicoquímicaFacultad de Farmacia y BioquímicaJunín 956(C1113AAD)Buenos Aires.Argentina.

The harmful effects of the reactive species may be due to the increase in their steady state concentration either by the enhancement of their production rates and/or the decrease of their consumption rate by antioxidant *** ascorbyl radical(A^(·))can be considered as a final product of radical oxidative transformations of ascorbate(AH^(-)).The ratio A^(·)content/AH^(-)content(A^(·)/AH^(-))has been widely used as an interesting tool to estimate mild to moderate oxidative transformations,providing a quick and simple method of diagnosis of stress in the hydrophilic cellular *** aim of this work was to summarize studies on the cellular oxidative condition in algae and invertebrates by assessing the A^(·)/AH^(-)ratio under environmentally changing *** use of indices of oxidative stress increasingly sensitive and,somewhat more specific,can bring a new light to the still unknown world of oxidative responses in marine organisms.

关键词： algae ascorbyl/ascorbate ratio invertebrates oxidative stress

Update on Fe-dependent oxidative metabolism in vivo:An integrative view

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BIOCELL 2016年第1期40卷 39-42页

作者： NATACHA E PILONI ELIZABETH ROBELLO JULIAN G BONETTO SUSANA PUNTARULO Instituto de bioquímica y Medicina molecular(IBIMOL)-Universidad de Buenos Aires(UBA) CONICETFisicoquímicaFacultad de Farmacia y BioquímicaJunín 956(C1113AAD)Buenos Aires.Argentina.

Fe is essential for human life because it constitutes the required cofactor for proteins of diverse biological ***,the development of oxidative stress by exposure to excessive Fe,share signaling pathways with other treatments including activation of redox-sensitive *** study was focused on the comparison on the effects of Fe in the brain and other organs in *** oxidative effects triggered by Fe overload strongly depend not only on the administration protocol,but also on the Fe-compound used,and the studied *** both the liver and the brain,Fe content drastically increased after Fe-dextran ***,the comparatively low lipid peroxidation in the brain as compared to the liver,suggested that Fe-dependent oxidative stress might involve mechanisms of different *** the brain,acute and subchronic administration of Fe-dextran triggered signaling processes that lead to the prevention of injury by the participation of catalase activity as an antioxidant *** brief summary opens a huge range of possible points of risk,as well as opportunities,to encounter situations in which the appropriate election of the Fe management protocol could be able of allow oxidative stress to exert beneficial effects.

关键词： experimental models Fe-overload oxidative stress