检索结果-南通市图书馆

Constructing efficient bacterial cell factories to enable one-carbon utilization based on quantitative biology:A review

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Quantitative Biology 2024年第1期12卷 1-14页

作者： Yazhen Song Chenxi Feng Difei Zhou Zengxin Ma Lian He Cong Zhang Guihong Yu Yan Zhao Song Yang Xinhui Xing School of Life Sciences Shandong Province Key Laboratory of Applied Mycologyand Qingdao International Center on Microbes Utilizing BiogasQingdao AgriculturalUniversityQingdaoChina Department of Chemical Engineering University of WashingtonSeattleWashingtonUSA Key Laboratory of Systems Bioengineering Ministry of EducationTianjin UniversityTianjinChina Key Laboratory of Industrial Biocatalysis Ministry of EducationDepartment of Chemical EngineeringTsinghua UniversityBeijingChina Center for Synthetic and Systems Biology Tsinghua UniversityBeijingChina Institute of Biopharmaceutical and Health Engineering Tsinghua Shenzhen International Graduate Schooland Insttute of Biomedical Health Technology and EngineeringShenzhen Bay LaboratoryShenzhenChina

Developing methylotrophic cell factories that can efficiently catalyze organic one-carbon(C1)feedstocks derived from electrocatalytic reduction of carbon dioxide into bio-based chemicals and biofuels is of strategic significance for building a carbon-neutral,sustainable economic and industrial *** the rapid advancement of RNA sequencing technology and mass spectrometer analysis,researchers have used these quantitative microbiology methods extensively,especially isotope-based metabolic flux analysis,to study the metabolic processes initiating from C1 feedstocks in natural C1-utilizing bacteria and synthetic C1 *** paper reviews the use of advanced quantitative analysis in recent years to understand the metabolic network and basic principles in the metabolism of natural C1-utilizing bacteria grown on methane,methanol,or *** acquired knowledge serves as a guide to rewire the central methylotrophic metabolism of natural C1-utilizing bacteria to improve the carbon conversion efficiency,and to engineer non-C1-utilizing bacteria into synthetic strains that can use C1 feedstocks as the sole carbon and energy *** progresses ultimately enhance the design and construction of highly efficient C1-based cell factories to synthesize diverse high value-added *** integration of quantitative biology and synthetic biology will advance the iterative cycle of understand–design–build–testing–learning to enhance C1-based biomanufacturing in the future.

关键词： ^(13)C-metabolic flux analysis methylotrophic cell factories one-carbon feedstock quantitative biology

Parameter estimation for whole-body kinetic model of FDG metabolism

在线全文

学校读者我要写书评

暂无评论

Progress in Natural Science:Materials International 2006年第11期16卷 1164-1170页

作者： CUI Yunfeng~1, BAI Jing~1**, CHEN Yingmao~2 and TIAN Jiahe~2(1. Department of biomedical Engineering, Tsinghua University, Beijing 100084, China 2. Department of Nuclear Medicine, General Hospital of PLA, Beijing 100853, China) Department of biomedical Engineering Tsinghua University Beijing 100084 China

Based on the radioactive tracer [ ~ 18 F]2-fluoro-2-deoxy-D-glucose （FDG）, positron emission tomography （PET）, and compartment model, the tracer kinetic study has become an important method to investigate the glucose metabolic kinetics in human body. In this work, the kinetic parameters of three-compartment and four-parameter model for the FDG metabolism in the tissues of myocardium, lung, liver, stomach, spleen, pancreas, and marrow were estimated through some dynamic FDG-PET experiments. Together with published brain and skeletal muscle parameters, a relatively complete whole-body model was presented. In the liver model, the dual blood supply from the hepatic artery and the portal vein to the liver was considered for parameter estimation, and the more accurate results were obtained using the dual-input rather than the single arterial-input. The established whole-body model provides the functional information of FDG metabolism in human body. It can be used to further investigate the glucose metabolism, and also be used for the simulation and visualization of FDG metabolic process in human body.

关键词： compartment model FDG PET parameter estimation.

Metabolomic Analysis of Serum Glycerophospholipid Levels in Eosinophilic and Neutrophilic Asthma

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

biomedical and Environmental Sciences 2019年第2期32卷 96-106页

作者： GAI Xiao Yan ZHANG Li Jiao CHANG Chun GUO Cheng Lin ABULIKEMU Mairipaiti LI Wen Xiong WANG Juan YAO Wan Zhen ZHANG Xu Department of Pulmonary and Critical Care Medicine Peking University Third Hospital Tianjin Key Laboratory of metabolic Diseases and Department of Physiology Tianjin Medical University

Objective To compare the serum glycerophospholipid levels in the inflammatory subtypes of asthma by using targeted metabolomic analysis. Methods Demographic and clinical data were collected from 51 patients with asthma between January 2015 and December 2015. Routine blood and sputum induction tests were performed. Eosinophilic asthma was defined as induced sputum containing ≥ 3% eosinophils, and neutrophilic asthma, as induced sputum containing ≥ 71% neutrophils. Serum metabolic glycerophospholipid profile was determined by liquid chromatography-mass spectrometry. Differences in glycerophospholipid levels between eosinophilic and non-eosinophilic asthma and between neutrophilic and non-neutrophilic asthma were analyzed using partial least squares discriminant analysis. Results The serum lysophosphatidylglycerol level was significantly higher in the group with ≥ 3% eosinophils in sputum than in the group with metabolic glycerophospholipid profile between the group with sputum neutrophils ≥ 71% and the group with sputum neutrophils < 71%. Conclusion Serum lysophosphatidylglycerol is produced abundantly in eosinophilic asthma and may be a biomarker of eosinophilic asthma. This information is helpful for identifying and tailoring treatment for the common asthma subtypes.

关键词： Eosinophilic asthma Neutrophilic asthma Glycerophospholipids Sputum induction Metabolomics

Structural,Functional,and Molecular imaging of Autism Spectrum Disorder

在线全文

学校读者我要写书评

暂无评论

Neuroscience Bulletin 2021年第7期37卷 1051-1071页

作者： Xiaoyi Li Kai Zhang Xiao He Jinyun Zhou Chentao Jin Lesang Shen Yuanxue Gao Mei Tian Hong Zhang Department of Nuclear Medicine and Medical PET Center The Second Hospital of Zhejiang University School of MedicineHangzhou 310009China Key Laboratory of Medical Molecular imaging of Zhejiang Province Hangzhou 310009China Key Laboratory for biomedical Engineering of Ministry of Education Zhejiang UniversityHangzhou 310027China Laboratory for Pathophysiological and Health Science RIKEN Center for Biosystems Dynamics ResearchHyogo 650-0047Japan Department of Surgical Oncology The Second Hospital of Zhejiang University School of MedicineHangzhou 310009China Institute of Nuclear Medicine and Molecular imaging Zhejiang UniversityHangzhou 310009China The College of biomedical Engineering and Instrument Science of Zhejiang University Hangzhou 310027China

Autism spectrum disorder(ASD)is a heterogeneous neurodevelopmental disorder associated with both genetic and environmental *** approaches have been widely employed to parse the neurophysiological mechanisms underlying ASD,and provide critical insights into the anatomical,functional,and neurochemical *** reviewed recent advances in neuroimaging studies that focused on ASD by using magnetic resonance imaging(MRI),positron emission tomography(PET),or single-positron emission tomography(SPECT).Longitudinal structural MRI has delineated an abnormal developmental trajectory of ASD that is associated with cascading neurobiological processes,and functional MRI has pointed to disrupted functional neural ***,PET and SPECT imaging have revealed that metabolic and neurotransmitter abnormalities may contribute to shaping the aberrant neural circuits of *** large-scale,multi-center,multimodal investigations are essential to elucidate the neurophysiological underpinnings of ASD,and facilitate the development of novel diagnostic biomarkers and better-targeted therapy.

关键词： Autism spectrum disorder Positron emission tomography Magnetic resonance imaging Molecular imaging Functional connectivity Serotonin Oxytocin

Quantitative proteomics revealed extensive microenvironmental changes after stem cell transplantation in ischemic stroke

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Frontiers of Medicine 2022年第3期16卷 429-441页

作者： Yao Chen Fahuan Song Mengjiao Tu Shuang Wu Xiao He Hao Liu Caiyun Xu Kai Zhang Yuankai Zhu Rui Zhou Chentao Jin Ping Wang Hong Zhang Mei Tian Department of Nuclear Medicine and Medical PET Center The Second Affiliated HospitalZhejiang University School of MedicineHangzhou 310009China Institute of Nuclear Medicine and Molecular imaging Zhejiang UniversityHangzhou 310009China Key Laboratory of Medical Molecular imaging of Zhejiang Province Hangzhou 310009China Shanxi Medical University Taiyuan 030001China Key Laboratory for biomedical Engineering of Ministry of Education Zhejiang UniversityHangzhou 310027China College of biomedical Engineering and Instrument Science Zhejiang UniversityHangzhou 310027China Department of Radiology Zhejiang HospitalHangzhou 310030China Department of PET Center The First Affiliated HospitalZhejiang University School of MedicineHangzhou 310006China

The local microenvironment is essential to stem cell-based therapy for ischemic stroke,and spatiotemporal changes of the microenvironment in the pathological process provide vital clues for understanding the therapeutic ***,relevant studies on microenvironmental changes were mainly confined in the acute phase of stroke,and long-term changes remain *** study aimed to investigate the microenvironmental changes in the subacute and chronic phases of ischemic stroke after stem cell ***,induced pluripotent stem cells(iPSCs)and neural stem cells(NSCs)were transplanted into the ischemic brain established by middle cerebral artery occlusion *** emission tomography imaging and neurological tests were applied to evaluate the metabolic and neurofunctional alterations of rats transplanted with stem *** proteomics was employed to investigate the protein expression profiles in iPSCs-transplanted brain in the subacute and chronic phases of *** with NSCs-transplanted rats,significantly increased glucose metabolism and neurofunctional scores were observed in iPSCs-transplanted *** proteomic data of iPSCs-transplanted rats identified a total of 39 differentially expressed proteins in the subacute and chronic phases,which are involved in various ischemic stroke-related biological processes,including neuronal survival,axonal remodeling,antioxidative stress,and mitochondrial function *** together,our study indicated that iPSCs have a positive therapeutic effect in ischemic stroke and emphasized the wide-ranging microenvironmental changes in the subacute and chronic phases.

关键词： ischemic stroke microenvironment induced pluripotent stem cells(iPSCs) positron emission tomography(PET) quantitative proteomics

Bow hunter’s syndrome successfully treated with a posterior surgical decompression approach:A case report and review of literature

在线全文

学校读者我要写书评

暂无评论

World Journal of Clinical Cases 2022年第14期10卷 4494-4501页

作者： NiccolòOrlandi Francesco Cavallieri Ilaria Grisendi Antonio Romano Reza Ghadirpour Manuela Napoli Claudio Moratti Matteo Zanichelli Rosario Pascarella Franco Valzania Marialuisa Zedde Department of biomedical Metabolic and Neural SciencesCenter for Neuroscience and NeurotechnologyUniversity of Modena and Reggio EmiliaModena 41121Italy Neurology Unit Neuromotor and Rehabilitation DepartmentAzienda USL-IRCCS di Reggio EmiliaReggio Emilia 42123Italy Clinical and Experimental Medicine PhD Program University of Modena and Reggio EmiliaModena 41121Italy Neurosurgery Unit Neuromotor and Rehabilitation DepartmentAzienda USL-IRCCS di Reggio EmiliaReggio Emilia 42123Italy Neuroradiology Unit Department of Diagnostic Imaging and Laboratory MedicineAzienda USL-IRCCS di Reggio EmiliaReggio Emilia 41123Italy

BACKGROUND Bow hunter’s syndrome(BHS)is a rare but surgically treatable cause of vertebrobasilar insufficiency due to dynamic rotational occlusion of the vertebral ***,patients present with posterior circulation transient ischaemic symptoms such as presyncope,syncope,vertigo,diplopia,and horizontal nystagmus,but irreversible deficits,including medullary and cerebellar infarctions,have also been *** SUMMARY A 70-year-old patient presented an acute onset of vertigo and gait instability triggered by right head *** medical history included previous episodes of unilateral left neck and occipital pain followed by light-headedness,sweating,and blurred vision when turning his head,and these episodes were associated with severe degenerative changes in the atlanto-dens and left atlanto-axial facet joints and right rotation of the C2 cervical *** magnetic resonance imaging revealed the presence of acute bilateral cerebellar ischaemic lesions,while static vascular imaging did not reveal any vertebral artery *** ultrasonography and angiography were performed and confirmed the presence of a dynamic occlusion of the vertebral artery V3-V4 segment when the head was rotated to the right secondary to left C1-C2 bone spur *** decompression led to complete resolution of paroxysmal symptoms without neurological *** BHS should be considered in cases of repeated posterior circulation transient ischaemic attack or ischaemic stroke,particularly when associated with high cervical spine abnormalities.

关键词： Bow hunter’s syndrome Stroke Non-invasive duplex ultrasonography Dynamic angiography Neurosurgery Case report

Cell-based drug delivery systems for biomedical applications

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Nano Research 2018年第10期11卷 5240-5257页

作者： Teng Li He Dong Can Zhang Ran Mo State Key Laboratory of Natural Medicines Jiangsu Key Laboratory of Drug Discovery for Metabolic Diseases Center of AdvancedPharmaceuticals and 8iomaterials China Pharmaceutical University Nanjing 210009 China

Spurred by numerous achievements in nanoscience and nanotechnology, the evolution of nanoparticulate drug delivery systems （nano-DDSs） is in its rapid growth period and attracting considerable attention due to their unique advantages in biomedical applications. Natural particulates ranging from mammalian cells to bacteria possess their own distinctive delivery processes and mechanisms, which inspires more design and development of cell-based DDSs by integrating the innate functions of cells with the nanoscale characteristics of nanoparticles. In this review article, we focus on the recent advances in cell-based DDSs for site-specific delivery of therapeutics and enhanced treatment of diseases. The promise and perils of cell-based DDSs are also discussed.

关键词： natural particulates nanotechnology bioinspired drug delivery biomedical application

Lipase-activated glycopeptide nano-assemblies as an antibiotic nano-adjuvant to inhibit Pseudomonas aeruginosa biofilm and enhance antibacterial activity

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China Chemistry 2022年第12期65卷 2538-2547页

作者： Yifei Song Jingkai Zhang Yue Fei Zhangjian Huang Xiaoxuan Liu Li-Li Li CAS Center for Excellence in Nanoscience CAS Key Laboratory for Biomedical Effects of Nanomaterials and NanosafetyNational Center for Nanoscience and Technology(NCNST)Beijing100190China College of Life Science and Technology Beijing University of Chemical TechnologyBeijing100029China State Key Laboratory of Natural Medicines and Jiangsu Key Laboratory of Drug Discovery for metabolic Diseases Center of Drug DiscoveryCenter of Advanced Pharmaceutics and BiomaterialsChina Pharmaceutical UniversityNanjing210009China

Bacterial infections,especially those caused by biofilms,pose a serious threat to public *** this work,we propose a glycopeptide-based antibiotic nano-adjuvant(GalAc-2) that can synergistically enhance the antibacterial activity of antibiotics through its biofilm inhibition *** glycopeptide nanoparticles can be specifically activated by bacterial infection overexpressed lipase and sequentially enlarged by glycosyl exposure on the *** stability and bonding affinity of the nanoparticles were enhanced through the increase in β-sheet *** bacterial growth inhibition rate of the mixture of levofloxacin(LEV) and GalAc-2 at 0.54 μg/m L was over sevenfold higher than that of LEV alone because of the electrostatic and multivalence Lec A interactions that inhibited biofilm formation on the bacterial ***,the mixture of the antibiotic with GalAc-2 significantly inhibited the abscess exposed to skin infection and significantly reduced the infiltration of immune *** believe that GalAc-2 can be further used to enhance broad-spectrum antibiotic sensitization and prolong the lifetime of antibiotics.

关键词： glycopeptide self-assembly antibiotic nano-adjuvant bacteria biofilm

metabolic reprogramming by Syntenin-1 directs RA FLS and endothelial cell-mediated inflammation and angiogenesis

在线全文

学校读者我要写书评

暂无评论

Cellular & Molecular Immunology 2024年第1期21卷 33-46页

作者： Anja Meyer Stephanie R.Zack Wes Nijim Adel Burgos Vishwa Patel Brian Zanotti Michael V.Volin M.Asif Amin Myles J.Lewis Costantino Pitzalis Shiva Arami Joseph A.Karam Nadera J.Sweiss Shiva Shahrara Jesse Brown VA Medical Center ChicagoILUSA Department of Medicine Division of RheumatologyThe University of Illinois at ChicagoChicagoILUSA Department of Microbiology and Immunology Midwestern UniversityDowners GroveILUSA Division of Rheumatology and Clinical Autoimmunity Center of Excellence University of MichiganAnn ArborMIUSA Centre for Experimental Medicine and Rheumatology William Harvey Research InstituteBarts and The London School of Medicine and DentistryQueen Mary University of LondonLondonUnited Kingdom Centre for Experimental Medicine&Rheumatology William Harvey Research InstituteQueen Mary University of London and Barts NIHR BRC&NHS TrustLondonUK Department of biomedical Sciences Humanitas Universityand Humanitas Research HospitalMilanItaly Department of Orthopedic Surgery the University of Illinois at ChicagoChicagoILUSA

A novel rheumatoid arthritis(RA)synovial fluid protein,Syntenin-1,and its receptor,Syndecan-1(SDC-1),are colocalized on RA synovial tissue endothelial cells and fibroblast-like synoviocytes(FLS).Syntenin-1 exacerbates the inflammatory landscape of endothelial cells and RA FLS by upregulating transcription of IRF1/5/7/9,IL-1β,IL-6,and CCL2 through SDC-1 ligation and HIF1α,or mTOR ***,Syntenin-1 orchestrates RA FLS and endothelial cell invasion via SDC-1 and/or mTOR *** Syntenin-1 reprogrammed endothelial cells,the dynamic expression of metabolic intermediates coincides with escalated glycolysis along with unchanged oxidative factors,AMPK,PGC-1α,citrate,and inactive oxidative ***,RA FLS rewired by Syntenin-1 displayed a modest glycolytic-ATP accompanied by a robust mitochondrial-ATP *** enriched mitochondrial-ATP detected in Syntenin-1 reprogrammed RA FLS was coupled with mitochondrial fusion and fission recapitulated by escalated Mitofusin-2 and DRP1 *** found that VEGFR1/2 and Notch1 networks are responsible for the crosstalk between Syntenin-1 rewired endothelial cells and RA FLS,which are also represented in RA *** to RA explants,morphological and transcriptome studies authenticated the importance of VEGFR1/2,Notch1,RAPTOR,and HIF1αpathways in Syntenin-1 arthritic mice and their obstruction in SDC-1 deficient ***,dysregulation of SDC-1,mTOR,and HIF1αnegated Syntenin-1 inflammatory phenotype in RA explants,while inhibition of HIF1αimpaired synovial angiogenic imprint amplified by *** conclusion,since the current therapies are ineffective on Syntenin-1 and SDC-1 expression in RA synovial tissue and blood,targeting this pathway and its interconnected metabolic intermediates may provide a novel therapeutic strategy.

关键词： Syntenin-1 Syndecan-1 RA FLS RA explants immunometabolism