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Photonics Research 2024年第4期12卷 821-832页

作者： WEI SHI YINGCHUAN HE JIANLIN WANG LULU ZHOU JIANWEI CHEN LIWEI ZHOU ZEYU XI ZHEN WANG KE FANG YIMING LI department of biomedical Engineering Southern University of Science and TechnologyShenzhen 518055China

Single-molecule localization microscopy(SMLM)enables three-dimensional(3D)investigation of nanoscale structures in biological samples,offering unique insights into their ***,traditional 3D super-resolution microscopy using high numerical aperture(NA)objectives is limited by imaging depth of field(DOF),restricting their practical application to relatively thin biological ***,we developed a unified solution for thick sample super-resolution imaging using a deformable mirror(DM)which served for fast remote focusing,optimized point spread function(PSF)engineering,and accurate aberration *** effectively correcting the system aberrations introduced during remote focusing and sample aberrations at different imaging depths,we achieved high-accuracy,large DOF imaging(~8μm)of the whole-cell organelles[i.e.,nuclear pore complex(NPC),microtubules,and mitochondria]with a nearly uniform resolution of approximately 35 nm across the entire cellular volume.

关键词： enable mirror resolution

Transient stimulated Raman scattering spectroscopy and imaging

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Light(Science & Applications) 2024年第4期13卷 654-663页

作者： Qiaozhi Yu Zhengjian Yao Jiaqi Zhou Wenhao Yu Chenjie Zhuang Yafeng Qi Hanqing Xiong National biomedical Imaging Center College of Future TechnologyPeking UniversityBeijing 100871China biomedical Engineering department College of Future TechnologyPeking UniversityBeijing 100871China

Stimulated Raman scattering(SRS)has been developed as an essential quantitative contrast for chemical imaging in recent ***,while spectral lines near the natural linewidth limit can be routinely achieved by state-of-the-art spontaneous Raman microscopes,spectral broadening is inevitable for current mainstream SRS imaging *** is because those SRS signals are all measured in the frequency *** is a compromise between sensitivity and spectral resolution:as the nonlinear process benefits from pulsed excitations,the fundamental time-energy uncertainty limits the spectral ***,the spectral range and acquisition speed are mutually *** we report transient stimulated Raman scattering(T-SRS),an alternative time-domain strategy that bypasses all these fundamental conjugations.T-SRS is achieved by quantum coherence manipulation:we encode the vibrational oscillations in the stimulated Raman loss(SRL)signal by femtosecond pulse-pair sequence excited vibrational wave packet *** Raman spectrum was then achieved by Fourier transform of the time-domain SRL *** all Raman modes are impulsively and simultaneously excited,T-SRS features the natural-linewidth-limit spectral line shapes,laser-bandwidth-determined spectral range,and improved ***~150-fs laser pulses,we boost the sensitivity of typical Raman modes to the sub-mM *** all-plane-mirror high-speed time-delay scanning,we further demonstrated hyperspectral SRS imaging of live-cell metabolism and high-density multiplexed imaging with the natural-linewidth-limit spectral resolution.T-SRS shall find valuable applications for advanced Raman imaging.

关键词： scattering excited mutually

Noncontact ultrasound sensing based on Mach-Zehnder homodyne interferometer for photoacoustic imaging

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Chinese Optics Letters 2024年第3期22卷 101-106页

作者：龙星胡毅成王一冰李长辉 department of biomedical Engineering College of Future TechnologyPeking UniversityBeijing 100871China National biomedical Imaging Center Peking UniversityBeijing 100871China

We present a novel noncontact ultrasound(US)and photoacoustic imaging(PAI)system,overcoming the limitations of traditional coupling *** a long coherent length laser,we employ a homodyne free-space Mach-Zehnder setup with zero-crossing triggering,achieving a noise equivalent pressure of 703 Pa at 5 MHz and a-6 dB bandwidth of 1 to8.54 *** address the phase uncertainty inherent in the homodyne *** the noncontact US probe enables photoacoustic computed tomography(PACT).Phantom studies demonstrate imaging performance and system stability,underscoring the potential of our system for noncontact US sensing and PAI.

关键词： noncontact ultrasound sensing photoacoustic imaging Mach–Zehnder interferometer

Biomimetic microchannel network with functional endothelium formed by sacrificial electrospun fibers inside 3D gelatin methacryloyl(GelMA)hydrogel models

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2024年第1期25卷 79-96页

作者： Haoyu SUN Haiyang MA Li WANG Yang LIU Tian HOU Wenjie TANG Qing YU Meiwen AN Meiling WEN Institute of biomedical Engineering College of Biomedical EngineeringTaiyuan University of TechnologyTaiyuan030024China

Three-dimensional(3D)hydrogel models play a crucial role in tissue engineering for promoting tissue regeneration.A biomimetic microchannel network system in the 3D hydrogel model is necessary for optimal cellular *** report describes the preparation of a biomimetic hydrogel scaffold with an internal microchannel network,using electrospinning techniques and the sacrificial template method for 3D cell *** and cavities were created within the gelatin methacryloyl(GelMA)hydrogel by sacrificing polyvinyl alcohol(PVA)electrospun fibers(>10µm),resulting in the creation of microvessel-like *** characterizations,swelling properties,and biodegradation analysis were conducted to investigate the feasibility of a biomimetic microchannel network hydrogel scaffold for 3D cell culture *** to pure GelMA hydrogel,the hydrogel with microchannels promoted cell proliferation,adhesion,and endothelial tube ***,the results confirmed that the biomimetic microchannel network scaffold had a major impact on the distribution and arrangement of human umbilical vein endothelial cells(HUVECs)and can enable the formation of artificial microvasculature by the culture of HUVECs and cell media perfusion.

关键词： Biomimetic scaffold Photocrosslinking Microchannel network Tissue engineering Artificial microvascular

Video-Based Deception Detection with Non-Contact Heart Rate Monitoring and Multi-Modal Feature Selection

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Journal of Beijing Institute of technology 2024年第3期33卷 175-185页

作者： Yanfeng Li Jincheng Bian Yiqun Gao Rencheng Song department of biomedical Engineering Hefei University of TechnologyHefei 230009China

Deception detection plays a crucial role in criminal *** contain a wealth of information regarding apparent and physiological changes in individuals,and thus can serve as an effective means of deception *** this paper,we investigate video-based deception detection considering both apparent visual features such as eye gaze,head pose and facial action unit(AU),and non-contact heart rate detected by remote photoplethysmography(rPPG)*** wrapper-based feature selection methods combined with the K-nearest neighbor(KNN)and support vector machine(SVM)classifiers are employed to screen the most effective features for deception *** evaluate the performance of the proposed method on both a self-collected physiological-assisted visual deception detection(PV3D)dataset and a public bag-oflies(BOL)*** results demonstrate that the SVM classifier with symbiotic organisms search(SOS)feature selection yields the best overall performance,with an area under the curve(AUC)of 83.27%and accuracy(ACC)of 83.33%for PV3D,and an AUC of 71.18%and ACC of 70.33%for *** demonstrates the stability and effectiveness of the proposed method in video-based deception detection tasks.

关键词： deception detection apparent visual features remote photoplethysmography non-contact heart rate feature selection

Benzydamine hydrochloride ameliorates ethanol-induced inflammation in RAW 264.7 macrophages by stabilizing redox homeostasis

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Asian Pacific Journal of Tropical Biomedicine 2024年第2期14卷 73-81,I0006-I0009页

作者： Tiasha Dasgupta Venkatraman Manickam department of biomedical Sciences School of Biosciences and TechnologyVellore Institute of TechnologyVellore 632014Tamil NaduIndia

Objective:To evaluate the protective effect of benzydamine hydrochloride against ethanol-induced oxidative stress and inflammation in RAW 264.7 ***:RAW 264.7 macrophages were treated with ethanol(100 mM)and benzydamine hydrochloride(7.5μM).The imflammatory status was confirmed by measuring pro-(TNF-αand IL-6)and anti-inflammatory(IL-10)cytokines through ELISA and RT-PCR *** oxygen species generation and mitochondrial membrane potential were investigated to study the protective role of benzydamine hydrochloride against ethanol-induced oxidative *** detection was also investigated using flow cytometry and acridine orange/ethidium bromide ***:Benzydamine hydrochloride significantly decreased the secretion of TNF-αand IL-6,as well as the generation of reactive oxygen species inside the cells,thereby stabilizing the mitochondrial membrane potential and reducing DNA *** ethanol-induced cellular necrosis was also reversed by the administration of benzydamine ***:Benzydamine hydrochloride ameliorates ethanol-induced cell apoptosis and inflammation in RAW macrophages.

关键词： Alcohol Benzydamine hydrochloride Inflammation Oxidative stress Apoptosis

Experimental realization of fractal fretwork metasurface for sound anomalous modulation

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Chinese Physics B 2024年第5期33卷 473-478页

作者：何佳杰于书萌江雪他得安 department of biomedical Engineering School of Information Science and TechnologyFudan UniversityShanghai 200433China Academy for Engineering and technology Fudan UniversityShanghai 200433China

Natural creatures and ancient cultures are full of potential sources to provide inspiration for applied *** by the fractal geometry in nature and the fretwork frame in ancient culture,here we design the acoustic metasurface to realize sound anomalous modulation,which manifests itself as an incident-dependent propagation behavior:sound wave propagating in the forward direction is allowed to transmit with high efficiency while in the backward direction is obviously *** quantitatively investigate the dependences of asymmetric transmission on the propagation direction,incident angle and operating frequency by calculating sound transmittance and energy *** compact fractal fretwork metasurface for acoustic anomalous modulation would promote the development of integrated acoustic devices and expand versatile applications in acoustic communication and information encryption.

关键词： acoustic metasurface fractal geometry sound anomalous modulation

An Overview of PRP-Delivering Scaffolds for Bone and Cartilage Tissue Engineering

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Journal of Bionic Engineering 2024年第2期21卷 674-693页

作者： Somayeh Baghersad Behzad Bolandi Rana Imani Shabnam Afaghi Samira Davoudinia department of Textile Engineering Amirkabir University of Technology(Tehran Polytechnic)Tehran159163-4311Iran department of biomedical Engineering Amirkabir University of Technology(Tehran Polytechnic)Tehran159163-4311Iran

Tissue engineering is nowadays an emerging approach that aims to replace or regenerate diseased or damaged organs with engineered constructs. Considering the key role of growth factors (GFs) in the tissue regeneration process, these biomolecules are considered an important part of the tissue engineering process, so the presence of growth factors in engineered scaffolds can accelerate tissue regeneration by influencing the behavior of cells. Platelet-rich plasma (PRP), as an autologous source of a variety of growth factors, is considered a therapeutic agent for the treatment of degenerative diseases. Regarding its ability to promote the healing process and tissue regeneration, PRP therapy has attracted great attention in bone and cartilage tissue engineering. Incorporating PRP and its derivatives into engineered scaffolds not only bioactivates the scaffold, but the scaffold matrix also acts as a sustained and localized growth factor release system. In addition, the presence of a scaffold can promote the bioactivity of GFs by providing an environment that facilitates their interaction, leading to enhanced effects compared to their free form. This review presents a brief overview of PRP's role in bone and cartilage tissue regeneration with the main focus on scaffold-mediated PRP delivery. In addition, the classification of platelet-rich products, current extraction techniques, terminology, and scaffold bioactivation methods are presented to provide a better understanding of the basics and the key aspects that may affect the effectiveness of therapy in bone and cartilage tissue engineering.

关键词： Platelet-rich plasma Growth factors delivery Scaffold Bone Cartilage

微泡辅助超声引导基因疗法的进展:机制与应用（英文）

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Science China Materials 2024年第9期67卷 2749-2766页

作者：铁真张书豪屈云凤王梦玄刘仁发孙德胜戴志飞 department of biomedical Engineering College of Future TechnologyNational Biomedical Imaging CenterState Key Laboratory of Vascular Homeostasis and RemodelingPeking University department of Ultrasonic Imaging Peking University Shenzhen Hospital

基因治疗作为当今流行的疗法之一,在各种疾病的治疗中取得了巨大的成功.然而,基因治疗的安全性、疗效和适用性也备受关注.微泡辅助超声引导下的基因治疗与传统方法相比具有以下优势:首先,这种策略能使治疗核酸有效地通过体内存在的天然... 详细信息

基因治疗作为当今流行的疗法之一,在各种疾病的治疗中取得了巨大的成功.然而,基因治疗的安全性、疗效和适用性也备受关注.微泡辅助超声引导下的基因治疗与传统方法相比具有以下优势:首先,这种策略能使治疗核酸有效地通过体内存在的天然屏障,如细胞膜屏障、血脑屏障、血视网膜屏障,可使药物富集在传统递送方法无法覆盖的病灶区域,进一步扩大了基因治疗的应用范围.其次,可以通过控制超声波的照射范围,将基因转染区域限制在病变区域,从而有效控制基因治疗脱靶的风险.此外,在超声成像和磁共振成像等多种成像技术的支持下,基因治疗过程可以在图像引导下进行,这将大大提高基因治疗的安全性.本综述重点介绍了超声波增强基因递送策略的进展,关注该策略的相关机制、衍生技术和相关前沿应用,并对该基因递送策略的局限性和未来前景进行了讨论.

关键词： gene therapy microbubble ultrasound cavitation image-guided gene therapy

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Ultra-high spatio-temporal resolution imaging with parallel acquisition-readout structured illumination microscopy(PAR-SIM)

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Light(Science & Applications) 2024年第6期13卷 1140-1158页

作者： Xinzhu Xu Wenyi Wang Liang Qiao Yunzhe Fu Xichuan Ge Kun Zhao Karl Zhanghao Meiling Guan Xin Chen Meiqi Li Dayong Jin Peng Xi department of biomedical Engineering College of Future TechnologyPeking UniversityBeijing100871China Wallace H.Coulter Dept.of biomedical Engineering Georgia Institute of Technology and Emory UniversityAtlanta30332GAUSA National biomedical Imaging Center Peking UniversityBeijing100871China department of biomedical Engineering College of EngineeringSouthern University of Science and TechnologyShenzhen518055GuangdongChina Airy Technologies Co. Ltd.Beijing100086China Eastern Institute for Advanced Study Eastern Institute of TechnologyNingboZhejiang315200China School of Life Science Peking UniversityBeijing100871China Institute for biomedical Materials and Devices(IBMD) Faculty of ScienceUniversity of Technology SydneySydneyNSW2007Australia

Structured illumination microscopy(SIM)has emerged as a promising super-resolution fluorescence imaging technique,offering diverse configurations and computational strategies to mitigate phototoxicity during real-time imaging of biological *** efforts to enhance system frame rates have concentrated on processing algorithms,like rolling reconstruction or reduced frame reconstruction,or on investments in costly sCMOS cameras with accelerated row readout *** this article,we introduce an approach to elevate SIM frame rates and region of interest(ROI)coverage at the hardware level,without necessitating an upsurge in camera expenses or intricate ***,parallel acquisition-readout SIM(PAR-SIM)achieves the highest imaging speed for fluorescence imaging at currently available detector *** using the full frame-width of the detector through synchronizing the pattern generation and image exposure-readout process,we have achieved a fundamentally stupendous information spatial-temporal flux of 132.9 MPixels·s^(−1),9.6-fold that of the latest techniques,with the lowest SNR of−2.11 dB and 100 nm ***-SIM demonstrates its proficiency in successfully reconstructing diverse cellular organelles in dual excitations,even under conditions of low signal due to ultra-short exposure ***,mitochondrial dynamic tubulation and ongoing membrane fusion processes have been captured in live COS-7 cell,recorded with PAR-SIM at an impressive 408 *** posit that this novel parallel exposure-readout mode not only augments SIM pattern modulation for superior frame rates but also holds the potential to benefit other complex imaging systems with a strategic controlling approach.

关键词： hardware illumination resolution