Benzydamine hydrochloride ameliorates ethanol-induced inflammation in RAW 264.7 macrophages by stabilizing redox homeostasis

作     者：Tiasha Dasgupta Venkatraman Manickam Tiasha Dasgupta;Venkatraman Manickam

作者机构：Department of Biomedical SciencesSchool of Biosciences and TechnologyVellore Institute of TechnologyVellore 632014Tamil NaduIndia 

出 版 物：《Asian Pacific Journal of Tropical Biomedicine》 (亚太热带生物医学杂志（英文版）)

年 卷 期：2024年第14卷第2期

页      面：73-81,I0006-I0009页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：supported by Indian Council of Medical Research(ICMR) the Government of India agency research grant(F.N.5/9/1328/2020-Nut) 

主　　题：Alcohol Benzydamine hydrochloride Inflammation Oxidative stress Apoptosis 

摘      要：Objective:To evaluate the protective effect of benzydamine hydrochloride against ethanol-induced oxidative stress and inflammation in RAW 264.7 ***:RAW 264.7 macrophages were treated with ethanol(100 mM)and benzydamine hydrochloride(7.5μM).The imflammatory status was confirmed by measuring pro-(TNF-αand IL-6)and anti-inflammatory(IL-10)cytokines through ELISA and RT-PCR *** oxygen species generation and mitochondrial membrane potential were investigated to study the protective role of benzydamine hydrochloride against ethanol-induced oxidative *** detection was also investigated using flow cytometry and acridine orange/ethidium bromide ***:Benzydamine hydrochloride significantly decreased the secretion of TNF-αand IL-6,as well as the generation of reactive oxygen species inside the cells,thereby stabilizing the mitochondrial membrane potential and reducing DNA *** ethanol-induced cellular necrosis was also reversed by the administration of benzydamine ***:Benzydamine hydrochloride ameliorates ethanol-induced cell apoptosis and inflammation in RAW macrophages.