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Neural Regeneration Research 2022年第11期17卷 2425-2426页

作者： Esperanza Martínez Sylvain Marcellini Juan Pablo Henríquez Neuromuscular Studies lab (NeSt lab) Group for the Study of Developmental Processes (GDeP) Department of Cell BiologyFaculty of Biological SciencesUniversidad de Concepción laboratory of Development and Evolution (LADE)

The neuromuscular junction(NMJ)is the peripheral synapse controlling muscle contraction and coordinated movement in a wide variety of *** humans,the mature NMJ is the primary target of morphological disassembly and fu... 详细信息

关键词： system contraction synapse

Chromium detoxification mechanism induced growth and antioxidant responses in vetiver(Chrysopogon zizanioides(L.) Roberty)

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Journal of Central South University 2019年第2期26卷 489-500页

作者： AN Wen-hui LI Wai-chin WU Chuan School of Metallurgy and Environment Central South University Plant Genetic Engineering and Molecular biology lab Department of Biotechnology Periyar University Department of Science and Environmental Studies The Education University of Hong Kong Department of biology Western Kentucky University Department of Marine Science University of Calcutta

This study investigated the chromium(Cr)detoxification mechanism-induced changes in growth and antioxidant defence enzyme activities in Chrysopogon *** growth decreased by 36.8%and 45.0%in the shoots and roots,respectively,in the 50 mg/L Cr *** accumulation was higher in root(9807μg/g DW)than in shoots(8730μg/g DW).Photosynthetic pigments and malondialdehyde content increased up to the 30 mg/L Cr treatment,whereas they declined at higher *** activity of superoxide dismutase(SOD),catalase(CAT)and peroxidase(POX)were increased significantly with increasing of Cr dose but slightly declined at higher *** banding patterns revealed the expression of multiple bands for SOD,CAT and POX enzymes,and the band intensity decreased at high doses of Cr *** results indicate that higher Cr doses increased the oxidative stress by over production of reactive oxygen species(ROS)in vetiver that had potential tolerance mechanism to Cr as evidenced by enhanced level of antioxidative enzymes,photosynthetic pigments,MDA ***,vetiver has evolved a mechanism for detoxification and accumulates higher concentration of toxic *** study provides a better understanding of how vetiver detoxifies Cr.

关键词： chromium Chrysopogon zizanioides detoxification mechanism antioxidant enzymes phytoremediation

Cell surface engineering with polyelectrolyte-stabilized magnetic nanoparticles： A facile approach for fabrication of artificial multicellular tissue-mimicking clusters

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Nano Research 2015年第8期8卷 2515-2532页

作者： Maria R. Dzamukova Ekaterina A. Naumenko Elvira V. Rozhina Alexander A. Trifonov Rawil F. Fakhrullin Bionanotechnology lab Institute of Fundamental Medicine and Biology Kazan Federal University Kreml uraml 18 Kazan 420008Republic of Tatarstan Russian Federation

Regenerative medicine requires new ways to assemble and manipulate cells for fabrication of tissue-like constructs. Here we report a novel approach for cell surface engineering of human cells using polymer-stabilized magnetic nanoparticles （MNPs）. Cationic polyelectrolyte-coated MNPs are directly deposited onto cellular membranes, producing a mesoporous semi-permeable layer and rendering cells magnetically responsive. Deposition of MNPs can be completed within minutes, under cell-friendly conditions （room temperature and physiologic media）. Microscopy （TEM, SEM, AFM, and enhanced dark-field imaging） revealed the intercalation of nanoparticles into the cellular microvilli network. A detailed viability investigation was performed and suggested that MNPs do not inhibit membrane integrity, enzymatic activity, adhesion, proliferation, or cytoskeleton formation, and do not induce apoptosis in either cancer or primary cells. Finally, magnetically functionalized cells were employed to fabricate viable layered planar （two-cell layers） cell sheets and 3D multicellular spheroids.

关键词： magnetic nanoparticles artificial multicelluar clusters magnetic modification

p53 INDEPENDENT G_1 ARREST AND APOPTOSIS INDUCED BY ADRIAMYCIN

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Chinese Medical Sciences Journal 1997年第2期12卷 71-75页

作者：邵志敏江明余黎明韩企夏沈镇宙 Department of Surgery Molecular Biology Lab Cancer Hospital Shanghai Medical University Shanghai 200032

The biological activity of adriamycin was investigated in human breast carcinoma (HBC) cells. Adri-amycin inhibited the growth of a number of HBC cell lines and induced G1 arrest followed by apoptosis. In MCF-7 cells that harbor wild-type p53, adriamycin-induced G1 arrest and apoptosis was accompanied by p53-independent regulation of WAF1/CIP1 as well as bax mRNA levels. In MDA-MB-231 cells which possess a mutant p53, adriamycin-induced G1 arrest and apoptosis was also associated with a cpncomitant up-regulation of WAF1/CIP1 mRNA while these cells did not express bax or bcl-2 messages. Thus, adriamycin induces G1 arrest and apoptosis via a unique pathway which appears to involve activation of downstream effectors of p53-independent manner.

关键词： breast cancer apoptosis p53

Anticancer activity of crude acetone and water extracts of Tulbaghia violacea on human oral cancer cells

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Asian Pacific Journal of Tropical Biomedicine 2018年第9期8卷 456-462页

作者： Samkeliso Takaidza Arumugam Madan Kumar Cornelius Cano Ssemakalu Nagabishek Sirpu Natesh Gayathri Karanam Michael Pillay Department of Biotechnology Vaal University of Technology Cancer biology lab Molecular and Nanomedicine Research Unit Sathyabama Institute of Science and Technology

Objective: To evaluate the anticancer activity of crude acetone and water leaf extracts of Tulbaghia violacea on a human oral cancer cell line(KB).Methods: The antioxidant activity of the leaf extracts was evaluated by using the DPPH assay while the anti-proliferative activity was assessed by using the MTT *** morphological characteristics of apoptotic cells were examined by using the dual acridine orange/ethidium bromide *** cytometry was used to evaluate the induction of multi-caspase activity and changes in the cell ***: The acetone and water extracts exhibited antioxidant activity in a concentration dependent *** extracts inhibited the growth of the KB cell line with IC_(50) values of 0.2 mg/mL and 1 mg/mL, respectively for acetone and *** changes such as cell shrinkage, rounding and formation of membrane blebs were observed in the treated *** acridine orange/ethidium bromide staining, the number of apoptotic cells increased as the concentration of the extracts *** activation of multi-caspase activity in KB cells treated with Tulbaghia violacea extracts was concentration dependent, leading to cell death by apoptosis and cell cycle arrest at the G_2/M ***: The acetone and water extracts of Tulbaghia violacea appear to have anti-cancer activity against human oral cancer cells and need to be investigated further.

关键词： Anticancer activity Antioxidant Apoptosis Caspase Cell cycle Tulbaghia

Unveiling the morphogenetic code:A new path at the intersection of physical energies and chemical signaling

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World Journal of Stem Cells 2021年第10期13卷 1382-1393页

作者： Riccardo Tassinari Claudia Cavallini Elena Olivi Valentina Taglioli Chiara Zannini Carlo Ventura National laboratory of Molecular biology and Stem Cell Engineering National Institute of Biostructures and Biosystems–ELDOR LABBologna 40129Italy

In this editorial,we discuss the remarkable role of physical energies in the control of cell signaling networks and in the specification of the architectural plan of both somatic and stem *** particular,we focus on the biological relevance of bioelectricity in the pattern control that orchestrates both developmental and regenerative *** this end,the narrative starts from the dawn of the first studies on animal electricity,reconsidering the pioneer work of Harold Saxton Burr in the light of the current *** finally discuss the most recent evidence showing that bioelectric signaling is an essential component of the informational processes that control pattern specification during embryogenesis,regeneration,or even malignant *** conclude that there is now mounting evidence for the existence of a Morphogenetic Code,and that deciphering this code may lead to unprecedented opportunities for the development of novel paradigms of cure in regenerative and precision medicine.

关键词： Physical energies Stem cells Bioelectricity Electromagnetic radiation Mechanical forces Morphogenetic code

Characterization of SoxB2 and SoxC genes in Amphi-oxus (Branchiostoma belcheri):Implications for their evolutionary conservation

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Science China(Life Sciences) 2009年第9期52卷 813-822页

作者： LIN YuShuang,CHEN DongYan,FAN QiuSheng & ZHANG HongWei Institute of Developmental biology,Life Science College,Key lab of Experimental Teratology of Ministry of Education,Shandong University,Jinan 250100,China Institute of Developmental biology Life Science College Key Lab of Experimental Teratology of Ministry of Education Shandong University Jinan China

Most Sox genes directly affect cell fate determination and differentiation. In this study,we isolated two Sox genes:SoxB2 and SoxC from amphioxus (Branchiostoma belcheri),the closest living invertebrate relative of the vertebrates. Alignments of SoxB2 and SoxC protein sequences and their vertebrate homologs show high conservation of their HMG domains. Phylogenic analysis shows that amphioxus SoxB2 and SoxC fall out of the vertebrate branches,suggesting that vertebrate homologs might arise from gene duplications during evolution. The two genes possess similar spatial and temporal expression patterns during embryogenesis and in adults. They are both maternally inherited. During neurulation,they are expressed in the neural ectoderm and archenterons. In adults,they are expressed not only in the nerve cord,but also in the gut,midgut diverticulum,gill and oocytes. These results suggest that amphioxus SoxB2 and SoxC might co-function and have conserved functions in the nervous system and gonads as their vertebrate homologs.

关键词： amphioxus SoxB2 SoxC neurulation gonads immunity evolution

Comparative analysis of SIMILAR to RCD ONE(SRO)family from tetraploid cotton species and their diploid progenitors depict their significance in cotton growth and development

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Journal of Cotton Research 2024年第1期7卷 45-57页

作者： SHABAN Muhammad TABASSUM Riaz RANA Iqrar Ahmad ATIF Rana Muhammad AZMAT Muhammad Abubakkar IQBAL Zubair MAJEED Sajid AZHAR Muhammad Tehseen Department of Plant Breeding and Genetics University of Agriculture FaisalabadSub-Campus BurewalaVehari 61010Pakistan Department of Chemistry and Biochemistry University of AgricultureFaisalabad 38400Pakistan Centre of Agricultural Biochemistry and Biotechnology University of AgricultureFaisalabad 38400Pakistan Department of Plant Breeding and Genetics University of AgricultureFaisalabad 38400Pakistan Precision Agriculture and Analytics lab National Centre in Big Data and Cloud ComputingCentre for Advanced Studies in Agriculture and Food SecurityUniversity of Agriculture FaisalabadFaisalabad 38400Pakistan Department of Plant Pathology University of CaliforniaDavisCA 95616USA State Key laboratory of Cotton biology Institute of Cotton ResearchChinese Academy of Agricultural SciencesAnyangHenan 455000China Federal Seed Certification and Registration Department Ministry of National Food Security and ResearchIslamabad 44090Pakistan School of Agriculture Sciences Zhengzhou UniversityZhengzhou 450000China

Background SRO(Similar to RCD1)genes family is largely recognized for their importance in the growth,develop-ment,and in responding to environmental ***,genome-wide identification and functional characteri-zation of SRO genes from cotton species have not been reported so *** A total of 36 SRO genes were identified from four cotton *** analysis divided these genes into three groups with distinct *** and chromosomal distribution analysis indicated uneven distribu-tion of GaSRO,GrSRO,GhSRO,and GbSRO genes on A2,D5 genomes,Gh-At,Gh-Dt,Gb-At,and Gb-Dt subgenomes,*** duplication analysis revealed the presence of six duplicated gene pairs among GhSRO *** promoter analysis,several elements responsive to the growth,development and hormones were found in GhSRO genes,implying gene induction during cotton growth and *** miRNAs responsive to plant growth and abiotic stress were predicted to target 12 GhSRO ***-specific expression profiling demonstrated the roles of GhSRO genes in one or more *** addition,specific expression pattern of some GhSRO genes dur-ing ovule development depicted their involvement in these developmental *** The data presented in this report laid a foundation for understanding the classification and functions of SRO genes in cotton.

关键词： Cotton SRO miRNAs Gene duplications Gene expression Ovule development

Protective Effect of Propyl Gallate Against Oxidized Low-Density Lipoprotein-Induced Injury of Endothelial Cells

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Chinese Journal of Integrative Medicine 2015年第4期21卷 299-306页

作者：马路朱晓法吴育云陈可冀史大卓殷惠军 Department of Geriatric Cardiology the Navy General Hospital of Chinese PLA The lab of Cardiovascular Molecular biology Xiyuan HospitalChina Academy of Chinese Medical Sciences

Objective： To evaluate the protective effect of propyl gallate （PG）, an alkyl ester of gallic acid which is an active ingredient of Radix Paeoniae, against oxidized low-density lipoprotein （ox-LDL）-induced apoptosis and death in endothelial cells （ECs） and to find out its preliminary mechanism. Methods： The cultured endothelial cells were divided into normal, model （ox-LDL）, control （fetal bovine serum）, PG high dose （20 p,g/mL）, PG middle dose （10 μg/mL）, and PG low dose （5 μg/mL） groups, each derived from three different pools of umbilical cords. The model of injured human umbilical vein endothelial cells （HUVECs） was induced by ox-LDL. The 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyl-2H-tetrazolium bromide （MTT） assay, Hoechst 33258 staining, flow cytometry and measurement of nitrogen monoxidum （NO） release were used to evaluate the protective effect of PG against ox-LDL-induced apoptosis and death in HUVECs. To find out the mechanism of this protective effect, the expression of endothelial nitric oxide synthase （eNOS） mRNA, eNOS protein expression, immunofluorescence of intracellular reactive oxygen species （ROS） and activities of malondialdehyde （MDA）, superoxidedismutase （SOD） and glutathione peroxidase （GPx） were observed. Results： PG significantly reduced ox-LDL-induced apoptosis and cell death. The percentage of cells death and apoptosis was significantly higher in the ox-LDL group than that in the control group （P〈0.05）. Compared with the control group, the cells death and apoptosis of PG group was no different （P〉0.05）. As compared with the ox-LDL group, results of the PG high dose group showed that cell viability was significantly increased （P〈0.05）, the level of NO release, expression of eNOS mRNA, densitometric value of eNOS protein expression, as well as the activities of SOD and GPx were all significantly higher （all P〈0.05）. Conclusion： PG could potentially serve as a novel endothelial protective agent against ox-LDL-induced injury of endothelial cell.

关键词： propyl gallate oxidized low-density lipoprotein endotheliocyte reactive oxygen species Radix Paeoniae Chinese medicine

Dropout training for SVMs with data augmentation

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Frontiers of Computer Science 2018年第4期12卷 694-713页

作者： Ning CHEN Jun ZHU Jianfei CHEN Ting CHEN MOE Key lab of Bioinformatics Bioinformatics Division and Center for Synthetic and Systems Biology TNLIST Tsinghua University Beijing 100084 China State Key lab of Intelligent Technology and Systems Department of Computer Science and Technology Tsinghua University Beijing 100084 China

Dropout and other feature noising schemes have shown promise in controlling over-fitting by artificially corrupting the training data. Though extensive studies have been performed for generalized linear models, little has been done for support vector machines （SVMs）, one of the most successful approaches for supervised learning. This paper presents dropout training for both linear SVMs and the nonlinear extension with latent representation learning. For linear SVMs, to deal with the intractable expectation of the non-smooth hinge loss under corrupting distributions, we develop an iteratively re-weighted least square （IRLS） algorithm by exploring data augmentation techniques. Our algorithm iteratively minimizes the expectation of a re- weighted least square problem, where the re-weights are analytically updated. For nonlinear latent SVMs, we con- sider learning one layer of latent representations in SVMs and extend the data augmentation technique in conjunction with first-order Taylor-expansion to deal with the intractable expected hinge loss and the nonlinearity of latent representa- tions. Finally, we apply the similar data augmentation ideas to develop a new IRLS algorithm for the expected logistic loss under corrupting distributions, and we further develop a non-linear extension of logistic regression by incorporating one layer of latent representations. Our algorithms offer insights on the connection and difference between the hinge loss and logistic loss in dropout training. Empirical results on several real datasets demonstrate the effectiveness of dropout training on significantly boosting the classification accuracy of both linear and nonlinear SVMs.

关键词： dropout SVMs logistic regression data aug- mentation iteratively reweighted least square