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Arabidopsis MADS-Box Transcription Factor AGL21 Acts as Environmental Surveillance of Seed Germination by Regulating ABI5 Expression

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Molecular Plant 2017年第6期10卷 834-845页

作者： Lin-Hui Yu Jie Wu Zi-Sheng Zhang Zi-Qing Miao Ping-Xia Zhao Zhen Wang Cheng-Bin Xiang School of Life sciences University of Science and Technology of China Hefei Anhui Province 230027 China shanghai center for Plant Stress Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200032 China

Seed germination is a crucial checkpoint for plant survival under unfavorable environmental conditions. Ab- scisic acid （ABA） signaling plays a vital role in integrating environmental information to regulate seed germination. It has been well known that MCMI/AGAMOUS/DEFICIENS/SRF （MADS）-box transcription factors are key regulators of seed and flower development in Arabidopsis. However, little is known about their functions in seed germination. Here we report that MADS-box transcription factor AGL21 is a negative regulator of seed germination and post-germination growth by controlling the expression of ABA-INSENSITIVE 5 （ABIb＇） in Arabidopsis. The AGL21-overexpressing plants were hypersensitive to ABA, salt, and osmotic stresses during seed germination and early post-germination growth, whereas ag121 mutants were less sensitive. We found that AGL21 positively regulated ABI5 expression in seeds. Consistently, genetic analyses showed that AGL21 is epistatic to ABI5 in controlling seed germination. Chromatin immunoprecipitation assays further demonstrated that AGL21 could directly bind to the ABI5 promoter in plant cells. Moreover, we found that AGL21 responded to multiple environmental stresses and plant hormones during seed germination. Taken together, our results suggest that AGL21 acts as a surveillance integrator that incorporates environmental cues and endogenous hormonal signals into ABA signaling to regulate seed germination and early post-germination growth.

关键词： MADS AGL21 seed germination ABA ABI5

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Fibroblast growth factor 7 is a nociceptive modulator secreted via large dense-core vesicles

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分子细胞生物学报:英文版 2015年第5期7卷 466-475页

作者： Hui Liu Qing-Feng Wu Jia-Yin Li Xing-Jun Liu Kai-Cheng Li Yan-Qing Zhong Dan Wu Qiong Wang Yin-Jing Lu Lan Bao Xu Zhang Institute of Neuroscience and State Key Laboratory of Neuroscience CAS Center for Excellence in Brain ScienceShanghai Institutes for Biological SciencesChinese Academy of SciencesShanghai 200031China State Key Laboratory of Cell Biology Institute of Biochemistry and Cell BiologyShanghai Institutes for Biological SciencesChinese Academy of SciencesShanghai 200031China School of Life Science and Technology ShanghaiTech UniversityShanghai 201210China

Fibroblast growth factor(FGF)7,a member of FGF family,is initially found to be secreted from mesenchymal cells to repair epithelial ***,its functions in the nervous system are largely *** present study showed that FGF7 was a neuromodulator localized in the large dense-core vesicles(LDCVs)in nociceptive ***7 was mainly expressed in small-diameter neurons of the dorsal root ganglion and could be transported to the dorsal spinal ***,FGF7 was mostly stored in LDCVs that did not contain neuropeptide substance *** recordings in the spinal cord slice showed that buffer-applied FGF7 increased the amplitude of excitatory post-synaptic current evoked by stimulating the sensory afferent *** tests showed that intrathecally applied FGF7 potentiated the formalin-induced acute nociceptive ***,both acute and inflammatory nociceptive responses were significantly reduced in Fgf7-deficient *** results suggest that FGF7 exerts an excitatory modulation of nociceptive afferent transmission.

关键词： fibroblast growth factor 7 dorsal root ganglion large dense-core vesicle nociceptive modulator inflammatory pain

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Journal of Genetics and Genomics 2017年第1期44卷 63-66页

作者： BO Yuan Tian-lin Cheng Kan Yang Xu Zhang Zilong Qiu Institute of Neuroscience Key Laboratory of Primate Neurobiology CAS Center for Excellence in Brain Science and Intelligence Technology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China Institute of Chemistry and Bioengineering Donghua University Shanghai 200051 China shanghai Clinical Research center Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China

Methyl-CpG binding protein 2 （MeCP2） has a crucial role in transcriptional regulation and neural development （Ausi6 et al., 2014）. Loss of function mutations of MECP2 in human lead to Rett syndrome （RTT）, a severe neurodevelopmental disorders （Amir et al., 1999）, whereas individuals with the chromosomal duplications containing the MECP2 locus showed severe autism-like symptoms （Ramocki et al., 2009）.

关键词： FGF Autism-related protein MeCP2 regulates FGF13 expression and emotional behaviors microRNAs

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Intermittent hypoxia attenuates ischemia/reperfusion induced apoptosis in cardiac myocytes via regulating Bcl-2/Bax expression

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Cell Research 2003年第5期13卷 385-391页

作者： JIAN WEN DONG, HAI FENG ZHU, WEI ZHONG ZHU, HAI LEI DING, TIE MIN MA, ZHAO NIAN ZHOU 1 Laboratory of Hypoxic Cardiovascular Physiology, shanghai institutes for biological sciences, Chinese Academy of sciences, shanghai 200031, China.2 Department of Physiology and Pathophysiology, Health Science center Peking University, Beijing 100083 China Laboratory of Hypoxic Cardiovascular Physiology Shanghai Institutes for Biological SciencesChinese Academy of Sciences Shanghai China Department of Physiology and Pathophysiology Health Science Center Peking University Beijing China

Intermittent hypoxia has been shown to provide myocardial protection against ishemia/reperfusion-induced injury. Cardiac myocyte loss through apoptosis has been reported in ischemia/reperfusion injury. Our aim was to investigate whether intermittent hypoxia could attenuate ischemia/reperfusion-induced apoptosis in cardiac myocytes and its potential mechanisms. Adult male Sprague-Dawley rats were exposed to hypoxia simulated 5000 m in a hypobaric chamber for 6 h/day, lasting 42 days. Normoxia group rats were kept under normoxic conditions. Isolated perfused hearts from both groups were subjected to 30 min of global ischemia followed by 60 min reperfusion. Incidence of apoptosis in cardiac myocytes was determined by terminal deoxynucleotidyl transferase mediated dUTP nick end labeling （TUNEL） and DNA agarose gel electrophoresis. Expressions of apoptosis related proteins, Bax and Bcl-2, in cytosolic and membrane fraction were detected by Western Blotting. After ischemia/reperfusion, enhanced recovery of ca

关键词： intermittent hypoxia apoptosis cardiac myocytes Bax Bcl-2.

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High-resolution gene profiling of infection process indicates serine metabolism adaptation of Fusarium graminearum in host

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Science Bulletin 2017年第11期62卷 758-760页

作者： Qing-Ning Xie Lei-Jie Jia Yan-Zhang Wang Ren-Tao Song Wei-Hua Tang School of Life sciences Shanghai University National Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant SciencesInstitute of Plant Physiology and EcologyShanghai Institutes for Biological SciencesChinese Academy of Sciences

Fusarium graminearum,an important fungal pathogen,can cause head blight,seedling blight and crown rot on wheat(Triticum aestivum),stalk rot and ear rot on maize(Zea mays)[1,2].Annually,billions of dollars are lost wor... 详细信息

关键词：禾谷镰刀菌感染过程基因分析高分辨率酸代谢脱氧雪腐镰刀菌烯醇菌丝宿主

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Progress in the detection of human genome structural variations

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Science China(Life sciences) 2009年第6期52卷 560-567页

作者： WU XueMei1,3 & XIAO HuaSheng1,2 1 center of Functional Genomics,Key Laboratory of System Biology,shanghai institutes for biological sciences,Chinese Academy of sciences,shanghai 200031,China 2 National Engineering center for Biochip at shanghai,shanghai 201203,China 3 Graduate School of the Chinese Academy of sciences,shanghai 200031,China center of Functional Genomics Key Laboratory of System Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China Graduate School of the Chinese Academy of sciences Shanghai China National Engineering center for Biochip at shanghai Shanghai China

The emerging of high-throughput and high-resolution genomic technologies led to the detection of submicroscopic variants ranging from 1 kb to 3 Mb in the human *** variants include copy number variations(CNVs),inversions,insertions,deletions and other complex rearrangements of DNA *** paper briefly reviews the commonly used technologies to discover both genomic structural variants and their potential ***,we highlight the array-based,PCR-based and sequencing-based assays,including array-based comparative genomic hybridization(aCGH),representational oligonucleotide microarray analysis(ROMA),multiplex amplifiable probe hybridization(MAPH),multiplex ligation-dependent probe amplification(MLPA),paired-end mapping(PEM),and next-generation DNA sequencing ***,we discuss the limitations and challenges of current assays and give advices on how to make the database of genomic variations more reliable.

关键词： structural variation cytogenetic microarray PCR paired end mapping(PEM) next-generation sequencing

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Construction of a mutant of Actinoplanes sp. N902-109 that produces a new rapamycin analog

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Chinese Journal of Natural Medicines 2018年第3期16卷 210-218页

作者： HUANG He GAO Ping ZHAO Qi HU Hai-Feng shanghai Institute of Pharmaceutical Industry Shanghai 201203 China Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200032 China

In the present study, we introduced point mutations into Ac_rap A which encodes a polyketide synthase responsible for rapamycin biosynthesis in Actinoplanes sp. N902-109, in order to construct a mutant with an inactivated enoylreductase(ER) domain, which was able to synthesize a new rapamycin analog. Based on the homologous recombination induced by double-strand breaks in chromosome mediated by endonuclease I-SceI, the site-directed mutation in the first ER domain of Ac_rapA was introduced using non-replicating plasmid pL YERIA combined with an I-SceI expression plasmid. Three amino acid residues of the active center, Ala-Gly-Gly, were converted to Ala-Ser-Pro. The broth of the mutant strain SIPI-027 was analyzed by HPLC and a new peak with the similar UV spectrum to that of rapamycin was found. The sample of the new peak was prepared by solvent extraction, column chromatography, and crystallization methods. The structure of new compound, named as SIPI-rapxin, was elucidated by determining and analyzing its MS and NMR spectra and its biological activity was assessed using mixed lymphocyte reaction(MLR). An ER domain–deficient mutant of Actinoplanes sp. N902-109, named as SIPI-027, was constructed, which produced a novel rapamycin analog SIPI-rapxin and its structure was elucidated to be 35, 36-didehydro-27-O-demethylrapamycin. The biological activity of SIPI-rapxin was better than that of rapamycin. In conclusion, inactivation of the first ER domain of rap A, one of the modular polyketide synthase responsible for macro-lactone synthesis of rapamycin, gave rise to a mutant capable of producing a novel rapamycin analog, 35, 36-didehydro-27-O-demethylrapamycin, demonstrating that the enoylreductase domain was responsible for the reduction of the double bond between C-35 and C-36 during rapamycin synthesis.

关键词： Actinoplanes sp。N902-109 Rapamycin 结构的类似物 Polyketide synthase Enoylreductase

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Association study of the single nucleotide polymorphisms in adiponectin-associated genes with type 2 diabetes in Han Chinese

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Journal of Genetics and Genomics 2009年第7期36卷 417-423页

作者： Yabing Wang Di Zhang Yun Liu Yifeng Yang Teng Zhao Jie Xu Sheng Li Zuofeng Zhang Guoyin Feng Lin He He Xu Institute for Nutritional sciences Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China Bio-X center Shanghai Jiao Tong University Shanghai 200030 China Department ofEpidemiology UCLA School of Public Health Los Angeles California 90095-1772 USA institutes of Biomedical sciences Fudan University Shanghai 200032 China

Single-nucleotide polymorphisms （SNPs） of ADIPOQ, ADIPOR1, and ADIPOR2 have been associated with type 2 diabetes mellitus （T2DM）, but there are many conflicting results especially in Chinese populations. To investigate the contribution of the adiponectin genes and their receptors to T2DM, a case-control study was performed and 11 SNPs ofADIPOQ, ADIPOR1, and ADIPOR2 were genotyped in 985 T2DM and 1,050 control subjects, rs 16861194 （-11426 A〉G） in the putative promoter of ADIPOQ was associated with T2DM （P = 0.007; OR = 1.29, 95% CI 1.08-1.55）. None of the other 10 SNPs were associated with T2DM in this study, although rs2241766 and rs1501299 were reported to be associated with T2DM in previous Chinese studies. There was also no significant difference found from the ADIPOQ haplotype analysis, which contains rs 16861194. In addition, we also assessed potential gene-gene interactions in three genes and no interactions were found. In conclusion, our results supported the ADIPOQ gene as a possible risk factor for type 2 diabetes in Han Chinese population.

关键词： adiponectin ADIPOQ ADIPORs SNP diabetes

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Learning to memorize: shedding new light on prefrontal functions

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Neuroscience Bulletin 2015年第2期31卷 242-244页

作者： Ning-long Xu Institute of Neuroscience State Key Laboratory of Neuroscience CAS Center for Excellence in Brain Science Shanghai Institutes for Biological Sciences Chinese Academy of Sciences

Working memory is one of the essential higher cognitive functions that actively holds behaviorally-relevant information essential for guiding subsequent actions. It includes subsystems that store and manipulate singlemode or multi-modal sensory information, e.g., spatial information, visual images, auditory scenes, olfactory objects, or any combination of these. In addition to merely holding a certain amount of information for a short period of time, as is generally believed, the cognitive processes involved are far more complex, including the executive and attentional control of short-term memory, permitting interim integration, and the processing, disposal, and retrieval of information. Evolution-wise, working memory is essential for the behavioral flexibility that allows humans and animals to quickly adapt to rapidly changing environments.

关键词： PFC Learning to memorize shedding new light on prefrontal functions

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Novel cyclophilin D inhibitors derived from quinoxaline exhibit highly inhibitory activity against rat mitochondrial swelling and Ca2+ uptake/release

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Acta Pharmacologica Sinica 2005年第10期26卷 1201-1211页

作者： Hong-xia GUO~(2,3), Feng WANG~(2,3), Kun-qian YU~3, Jing CHEN~3, Dong-lu BAI~3, Kai-xian CHEN~3, Xu SHEN~(3,4,5), Hua-liang JIANG~(3,4,5) ~3 Drug Discovery and Design center, State Key Laboratory of Drug Research, shanghai Institute of Materia Medica, shanghai institutes for Biologieal sciences Graduate School of the Chinese Academy of sciences, Chinese Academy of sciences, shanghai 201203, China ~4 School of Pharmacy, East China University of Science and Technology shanghai 200237, China Drug Discovery and Design center State Key Laboratory of Drug Research Shanghai Institute of Materia Medica Shanghai Institutes for Biological Sciences Graduate School of the Chinese Academy of Sciences Shanghai 201203 China.

Aim: To investigate methods for identifying specific cyclophilin D （CypD） inhibitors derived from quinoxaline, thus developing possible lead compounds toinhibit mitochondrial permeability transition （MPT） pore opening. Methods: Kinetic analysis of the CypD/inhibitor interaction was quantitatively performed byusing surface plasmon resonance （SPR） and fluorescence titration （FT） ***50values of these inhibitors were determined by PPIase inhibition activity ***: All the equilibrium dissociation constants （KD） of the seven compoundsbinding to CypD were below 10 μmol/L. The IC50values were all consistent withthe SPR and FT results. Compounds GW2, 5, 6, and 7 had high inhibition activitiesagainst Ca2+-dependent rat liver mitochondrial swelling and Ca2+uptake/*** GW5 had binding selectivity for CypD over CypA. Conclusion: Theagreement between the measured IC50values and the results of SPR and FT suggests that these methods are appropriate and powerful methods for fyingCypD inhibitors. The compounds we screened using these methods （GW1-7） arereasonable CypD inhibitors. Its potent ability to inhibit mitochondrial swelling and the binding selectivity of GW5 indicates that GW5 could potentially be used for inhibiting MPT pore opening.

关键词： cyclophilin quinoxalines surface plasmon resonance mitochondrial permeability transition: fluorescence titration inhibitor

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