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The new kid on the block in insect pest management:sprayable RNAi goes commercial

Science China(Life Sciences) 2024年第8期67卷 1766-1768页

作者： Junjie Yan Ralf Nauen Stuart Reitz Andrei Alyokhin Jiang Zhang David Mota-Sanchez Yonggyun Kim Subba Reddy Palli Silvia I.Rondon Brian A.Nault Juan Luis Jurat-Fuentes Michael S.Crossley William E.Snyder Angharad M.R.Gatehouse Myron P.Zalucki Bruce E.Tabashnik Yulin Gao State Key Laboratory for Biology of plant disease and Insect pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193China Bayer AG Crop Science DivisionR&DPest ControlMonheim 40789Germany Department of Crop and Soil Science Oregon State UniversityOntario 97914USA School of Biology and Ecology University of MaineOrono 04469USA Shenzhen Branch Guangdong Laboratory of Lingnan Modern AgricultureKey Laboratory of Synthetic BiologyMinistry of Agriculture and Rural AffairsAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518000China Department of Entomology Michigan State UniversityEast Lansing 48864USA Department of plant Medicals Andong National UniversityAndong 36729Republic of Korea Department of Entomology University of KentuckyLexington 40503USA Oregon State University Oregon Integrated Pest Management CenterCorvallis 97838USA Department of Entomology Cornell UniversityCornell AgriTechGeneva 14456USA Department of Entomology and plant Pathology University of TennesseeKnoxville 37996USA Department of Entomology and Wildlife Ecology University of DelawareNewark 19716USA Department of Entomology University of GeorgiaAthensGeorgia 30602USA School of Natural and Environmental Sciences Newcastle UniversityNewcastle upon TyneNE17RUUK School of the Environment University of QueenslandBrisbane 4072Australia Shandong engineering research center for Environment-Friendly Agricultural pest Management College of Plant Health and MedicineQingdao Agricultural UniversityQingdao 266109China Department of Entomology University of ArizonaTucson 85721USA National center of Excellence for Tuber and Root Crop research Chinese Academy of Agricultural SciencesBeijing 100081China

Insect pest damage to crops is a threat to global food security(Tilman et al.,2011).Climate change,the evolution of insecticide resistance,and the phasing out of insecticides due to environmental and safety concerns exacerbate this *** urgently need safe and effective crop protection tools to sustainably generate yields that meet ever-increasing global demand.

关键词： crops damage pest

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Efficient genome editing in rice with miniature Cas12f variants

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aBIOTECH 2024年第2期5卷 184-188页

作者： Zhengyan Ye Yuanyan Zhang Shiqi He Shaokang Li Longjiong Luo Yanbiao Zhou Junjie Tan Jianmin Wan Sanya Institute of Nanjing Agricultural University State Key Laboratory of Crop Genetics&Germplasm Enhancement and UtilizationProvince and Ministry Co-sponsored Collaborative Innovation Center for Modern Crop ProductionNanjing Agricultural UniversityNanjing 210095China Zhongshan Biological Breeding Laboratory No.50 Zhongling StreetNanjing 210014China Key Laboratory of Southern Rice Innovation&Improvement Ministry of Agriculture and Rural Affairs/Hunan Engineering Laboratory of Disease and Pest Resistant Rice BreedingYuan Longping High-Tech Agriculture Co.LtdChangsha 410001China

Genome editing,particularly using the CRISPR/Cas system,has revolutionized biological research and crop *** the widespread use of CRISPR/Cas9,it faces limitations such as PAM sequence requirements and challenges in delivering its large protein into plant *** hypercompact Cas12f,derived from Acidibacillus sulfuroxidans(AsCas12f),stands out due to its small size of only 422 amino acids and its preference for a T-rich motif,presenting advantageous features over ***,its editing efficiency is extremely low in *** studies have generated two AsCas12f variants,AsCas12f-YHAM and AsCas12f-HKRA,demonstrating higher editing efficiencies in mammalian cells,yet their performance in plants remains *** this study,through a systematic investigation of genome cleavage activity in rice,we unveiled a substantial enhancement in editing efficiency for both AsCas12f variants,particularly for AsCas12f-HKRA,which achieved an editing efficiency of up to 53%.Furthermore,our analysis revealed that AsCas12f predominantly induces deletion in the target DNA,displaying a unique deletion pattern primarily concentrated at positions 12,13,23,and 24,resulting in deletion size mainly of 10 and 11 bp,suggesting significant potential for targeted DNA deletion using *** findings expand the toolbox for efficient genome editing in plants,offering promising prospects for precise genetic modifications in agriculture.

关键词： CRISPR/Cas Cas12 AsCas12f Rice Genome editing

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In vitro Germination and Micropropagation of Aconitum vilmorinianum:An Important Medicinal plant in China

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Phyton-International Journal of Experimental Botany 2022年第10期91卷 2331-2348页

作者： Zongmin Mou Fei Ye Fangchen Shen Dake Zhao School of Ecology and Environmental Science Yunnan UniversityKunming650504China biocontrol engineering research center of plant disease and pest Yunnan UniversityKunming650504China biocontrol engineering research center of Crop disease and pest Yunnan UniversityKunming650504China Kunming Municipal Hospital of Traditional Chinese Medicine Kunming650500China

Aconitum vilmorinianum,a well-known traditional Chinese herb,is recently being threatened by overexploitation and environment *** study was conducted to provide propagation methods through in vitro germination and explant *** was stimulated up to 66.00%on Murashige and Skoog(MS)medium containing 2.0 mg L^(−1)6-benzylaminopurine(BAP),0.1 mg L^(−1)1-napthaleneacetic acid(NAA),and 30 g L^(−1)*** bacteria(Pantoea agglomerans,Erwinia persicina,and Pseudomonas tolaasii)would be responsible for consistent contamination during *** latter two were effectively eradicated after *** influence of explant types and hormone combinations on direct and indirect organogenesis was evaluated in the present *** frequency of shoot induction from axillary bud explants was 100%on the MS fortified with 2.0 mg L^(−1)BAP and 0.3 mg L^(−1)*** multiplication was optimized on MS medium supplemented with 0.1 mg L^(−1)thidiazuron(TDZ)and 0.1 mg L^(−1)*** callus induction percentage(96.67%)was obtained from stem segments on MS medium with 2.0 mg L^(−1)2,4-D,then successfully regenerated into shoots on MS medium in the presence of 0.1 mg L^(−1)TDZ and 0.2 mg L^(−1)*** present work could be useful for the utilization and conservation of this valuable species.

关键词： Aconitum vilmorinianum seed surface bacteria in vitro multiplication organogenesis

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Online diagnosis platform for tomato seedling diseases in greenhouse production

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International Journal of Agricultural and Biological engineering 2024年第1期17卷 80-89页

作者： Xin Jin Xiaowu Zhu Jiangtao Ji Mingyong Li Xiaolin Xie Bo Zhao College of Agricultural Equipment engineering Henan University of Science and TechnologyLuoyang 471003HenanChina Science&Technology Innovation center for Completed Set Equipment Longmen LaboratoryLuoyang 471023HenanChina Institute of Mechatronics Technology and Application China Mechanized Agriculture Science Research Institute Group Co.LtdBeijing 100020China

The facility-based production method is an important stage in the development of modern agriculture,lifting natural light and temperature restrictions and helping to improve agricultural production *** address the problems of difficulty and low accuracy in detecting pests and diseases in the dense production environment of tomato facilities,an online diagnosis platform for tomato plant diseases based on deep learning and cluster fusion was proposed by collecting images of eight major prevalent pests and diseases during the growing period of tomatoes in a facility-based *** diagnostic platform consists of three main parts:pest and disease information detection,clustering and decision-making of detection results,and platform diagnostic ***,based on the You Only Look Once(YOLO)algorithm,the key information of the disease was extracted by adding attention module(CBAM),multi-scale feature fusion was performed using weighted bi-directional feature pyramid network(BiFPN),and the overall construction was designed to be compressed and lightweight;Secondly,the k-means clustering algorithm is used to fuse with the deep learning results to output pest identification decision values to further improve the accuracy of identification applications;Finally,a detection platform was designed and developed using Python,including the front-end,back-end,and database of the system to realize online diagnosis and interaction of tomato plant pests and *** experiment shows that the algorithm detects tomato plant diseases and insect pests with mAP(mean Average Precision)of 92.7%,weights of 12.8 Megabyte(M),inference time of 33.6 *** with the current mainstream single-stage detection series algorithms,the improved algorithm model has achieved better performance;The accuracy rate of the platform diagnosis output pests and diseases information of 91.2%for images and 95.2%for *** is a great significance to tomato pest control research and t

关键词： pest and disease detection YOLO diagnosis platform k-means clustering facility production base

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Characterization of the voltage-gated sodium channel of the Asian citrus psyllid, Diaphorina citri

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Insect Science 2017年第1期24卷 47-59页

作者： Bin Liu Monique R. Coy Jin-Jun Wang Lukasz L. Stelinski Entomology and Nematology Department Citrus Research and Education Center University of Florida Florida USA Key Laboratory of Entomology and pest Control engineering College of Plant Protection Southwest University Chongqing China

The Asian citrus psyllid, Diaphorina citri Kuwayama （Hemiptera： Liviidae）, is an important insect pest of citrus. It is the vector of ＇Candidatus＇ Liberibacter asiaticus, a phloem-limited bacterium that infects citrus, resulting in the disease Huanglongbing （HLB）. disease management relies heavily on suppression of D. cirri populations with insecticides, including pyrethroids. In recent annual surveys to monitor insecticide resistance, reduced susceptibility to fenpropathrin was identified in several field populations olD. citri. The primary target ofpyrethroids is the voltage-gated sodium channel （VGSC）. The VGSC is prone to target-site insensitivity because of mutations that either reduce pyrethroid binding and/or alter gating kinetics. These mutations, known as knockdown resistance or kdr, have been reported in a wide diversity of arthropod species. Alternative splicing, in combination with kdr mutations, has been also associated with reduced pyrethroid efficacy. Here we report the molecular characterization of the VGSC in D. citri along with a survey of alternative splicing across developmental stages of this species. Previous studies demonstrated that D. citri has an exquisite enzymatic arsenal to detoxify insecticides resulting in reduced efficacy. The results from the current investigation demonstrate that target-site insensitivity is also a potential basis for insecticide resistance to pyrethroids in D. citri. The VGSC sequence and its molecular characterization should facilitate early elucidation of the underlying cause of an established case of resistance to pyrethroids. This is the first characterization of a VGSC from a hemipteran to this level of detail, with the majority of the previous studies on dipterans and lepidopterans.

关键词： citrusgreening diseasemanagement knockdownresistance(kdr) target-site insensitivity

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Characterization of the microbial community response to replant diseases in peach orchards

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Journal of Integrative Agriculture 2023年第4期22卷 1082-1092页

作者： LI Wei-hua CHEN Peng WANG Yu-zhu LIU Qi-zhi Laboratory of Entomology and Nematology College of Plant ProtectionChina Agricultural UniversityBeijing 100193P.R.China State Key Laboratory of Integrated Management of pest Insects and Rodents Institute of ZoologyChinese Academy of SciencesBeijing 100101P.R.China Key Laboratory of Natural Enemies Insects of Ministry of Agriculture and Rural Affairs Shandong Provincial Engineering Technology Research Center on Biocontrol of Crop Diseases and Insect PestInstitute of Plant ProtectionShandong Academy of Agricultural SciencesJinan 250100P.R.China Institute of Forestry and Pomology Beijing Academy of Agriculture and Forestry SciencesBeijing 100093P.R.China

This study attempted to monitor the development of microbial communities and reveal the correlation between the soil microbial community and soil nutrient factors over different years following the replanting of peach *** replanted soil(RS)and nonreplanted soil(NRS)were collected from peach orchards with different growth years(1,3,5,7,9,11,and 13 years)in the same *** soil bacterial and fungal community diversities were analyzed by high-throughput sequencing *** analysis(RDA)was used to show the correlation between the soil microbial community and environmental *** alpha diversities of the bacterial and fungal communities indicated that RS contained a higher abundance of bacterial and fungal operational taxonomic units(OTUs)than *** and ANOSIM analyses showed that the soil bacterial and fungal communities were significantly(Pplanting years,and that the main changes occurred in the first and ninth planting *** presence of the bacterial orders Sphingobacteriales,Burkholderiales and Actinomycetales changed significantly after *** bacteria associated with bioremediation,such as Burkholderiales and Intrasporangiaceae,and some harmful pathogens,such as Penicillium and Ophiostomatales,significantly increased after replanting(LDA score>3.0).In addition,the soil nutrient contents were lower in RS than in NRS in the early stage(1–5 years),and the RDA showed that bacterial and fungal phyla are closely associated with environmental variables,including the potential of hydrogen(pH),ammonium nitrogen(AN),available phosphorus(AP)and available potassium(AK).These results lead to a deeper understanding of the microbial responses to replanting in peach orchards.

关键词： replant disease complex syndrome microbial community high-throughput sequencing environmental variables

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Effects of sesame nectar on longevity and fecundity of seven Lepidoptera and survival of four parasitoid species commonly found in agricultural ecosystems

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Journal of Integrative Agriculture 2017年第11期16卷 2534-2546页

作者： LIU Kai ZHU Ping-yang Lü Zhong-xian CHEN Gui-hua ZHANG Jing-ming Lü Yao-bing LU Yan-hui State Key Laboratory Breeding Base for Zhejiang Sustainable pest and disease Control Institute of Plant Protection and Microbiology Zhejiang Academy of Agricultural Sciences Hangzhou 310021 P.R. China State Key Laboratory of biocontrol School of Life Sciences Sun Yat-sen University Guangzhou 510275 P.R. China Jinhua plant Protection Station Jinhua 321017 P.R. China School of Agricultural and Wine Sciences Charles Sturt University New South Wales 2800 Australia

Ecological engineering involves the use of plants to promote establishment, survival and efficiency of natural enemies in agricultural systems. Some plant species may be hosts or provide resources to some pest species. We assessed the risks and benefits of sesame （Sesamum indicum L.）, as a nectar source for seven economically important Lepidopteran pest and four parasitoid species in a range of vegetable crop systems. Our results showed that the mean Iongevities of arthropod parasitoids Pteromalus puparum （L.）, Encarsia sophia （Girault ＆ Dodd） and male Microplitis tuberculifer （Wesmael） were significantly extended when fed on sesame flowers compared to the water control. Sesame flowers had no effect on adult Iongevities and fecundities of six out of the seven Lepidoptera pest species tested except Plutella xyllostella （L.） females laid more eggs when fed on sesame flowers. It is likely that the increased fecundity is due to accessibility to nectar at the bottom of corolla because of their smaller body sizes. Our findings provide a first step towards better understanding of the risks and benefits of using sesame to implement ecological engineering for the management of vegetable pests.

关键词： Lepidopteran pests natural enemies flowering plant risks and benefits assessment ecological engineering

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Transcriptome Analysis of Female and Male Henosepilachna vigintioctopunctata(Coleoptera:Coccinellidae)Identifies Sex-related Genes

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Horticultural plant Journal 2021年第2期7卷 142-148页

作者： Wei Guo Zhuoqi Liu Jiaqi Yu Jing Lü Xiaoguo Jiao Lan Kong Chunxiao Yang Huipeng Pan Key Laboratory of Bio-pesticide Innovation and Application of Guangdong Province Engineering Technology Research Center of Agricultural Pest Biocontrol of Guangdong ProvinceSouth China Agricultural UniversityGuangzhou 510642China State Key Laboratory of Biocatalysis and Enzyme engineering Center for Behavioral Ecology&EvolutionSchool of Life SciencesHubei UniversityWuhan 430062China Department of Computer Science Eastern Kentucky UniversityRichmond KY 40475USA State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources South China Agricultural UniversityGuangzhou 510642China

Henosepilachna vigintioctopunctata is an emerging horticultural pest of Solanaceae plants in ***,we employed transcriptome sequencing of three female and three male *** libraries to identify sex-related *** recorded 281599294 bp of clean reads,and de novo assembly generated 85206 unigenes,with N_(50) of 1311 bp and average length of 763 *** found 5002 genes highly expressed in males and 2179 genes highly expressed in *** profiles of six unigenes specific to females,six unigenes specific to males,and ten unigenes common to both sexes were verified using semi-quantitive RT-PCR;results showed expression levels of these 22 unigenes were relatively consistent with the transcriptome *** all unigenes as references,we identified 6657 putative simple sequence *** transcriptome sequences and gene expression profiles of female and male *** help explore genetic mechanisms of sexual dimorphism.

关键词： Henosepilachna vigintioctopunctata sex RNA-seq transcriptome annotation differential expression

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Tri-milR29 from Trichoderma breve Targets Chitin Synthase Gene in Botrytis cinerea and its Enhanced disease Control Efficacy through Nanodelievery-base Application

Tri-milR29 from Trichoderma breve Targets Chitin Synthase Ge...

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中国植物病理学会2024年学术年会

作者：刘震 Raja Asad Ali Khan WANG Rui LI Yuejiao 刘铜 School of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication) Hainan University Key Laboratory of Green Prevention and Control of Tropical diseases and pests Ministry of Education (School of Tropical Agriculture and Forestry)Hainan University engineering center of Agricultural Microbial Preparation research and Development of Hainan (Hainan University)

In the process of mycoparasitism,a form of biological control that one fungus attacking another fungus,the exchange of biologically active molecules such as RNAs may occur,playing an important role in *** these molecules,microRNAs(miRNAs) can inhibit translation and regulate gene expression at the post-transcriptional level through complementary base pairing,thereby degrading target mRNAs to prevent ***,a well-known mycoparasitic fungus,is one of the most studied biocontrol *** recent years,small RNAs(named milRNA) have also been discovered in ***,the role of Trichoderma RNAs in their mycoparasitic activity and their targets in pathogenic fungi still needs further *** study verified the cross-species regulatory role of Trichoderma-T069 miRNA,named *** demonstrated that Tri-milR29 from T069 was transferred to ***,a pathogenic fungus,where it targeted the chitin synthase gene,BcCHS7,resulting in a marked decrease in its ***-species transportation of sRNAs may occur through extracellular vesicles(EVs) ***,more research is needed on EVs in *** a series of experiments,we verified that the Tri-milR29 from T069 was transferred to *** through the EVs *** sRNA can effectively prevent and control plant diseases by targeting specific genes in pathogenic microbes through RNA ***,their susceptibility to nuclease degradation,off-target effects,and their low absorption efficiency by target pathogens limit their practical ***,the development of suitable delivery systems to protect and deliver sRNA is *** developed a carbon nanomaterial-based delivery system that enhances the stability and absorption of TrimilR29 by ***,increasing antifungal *** nano-delivered Tri-milR29 significantly lowered the disease incidence caused by *** on various fruits and tomato plants.

关键词： microRNA extracellular vesicles biocontrol mycoparasitism pathogen

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Fermentation, formulation and evaluation of PGPR Bacillus subtilis isolate as a bioagent for reducing occurrence of peanut soil-borne diseases

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Journal of Integrative Agriculture 2019年第9期18卷 2080-2092页

作者： Abdel-Gayed M.Ahmad Abo-Zaid G.Attia Matar S.Mohamed Hafez E.Elsayed Onion Garlic and Oil Crops Research DepartmentPlant Pathology Research InstituteAgricultural Research CenterGiza 12619Egypt Bioprocess Development Department Genetic Engineering and Biotechnology Research InstituteCity of Scientific Research and Technological ApplicationsNew Borg El-Arab CityAlexandria 21934Egypt Chemical engineering Department Faculty of EngineeringJazan UniversityJazan 45142Saudi Arabia plant Protection and Biomolecular Diagnosis Department Arid Lands Cultivation Research InstituteCity of Scientific Research and Technological ApplicationsNew Borg El-Arab CityAlexandria 21934Egypt

Four isolates of Bacillus subtilis coded,B4,B7,B8 and B10 were examined as biocontrol agents for their abilities and antagonistic effect on the in vitro growth of certain phytopathogenic fungi of peanut,Rhizoctonia solani and Sclerotium *** subtilis isolate B4(GenBank accession ***150884)was the highly effective one for inhibiting the fungal mycelial *** fermentation of *** isolate B4 was carried out and the maximum biomass achieved was 4.53 g L-1 at 11 *** subtilis isolate B4 was formulated and evaluated as a biofungicide to reduce peanut soil-borne diseases under greenhouse and field conditions at the side of Rizolex-T(fungicide)as *** by formulated plant growth-promoting rhizobacteria(PGPR)*** B4 and Rizolex-T in a soil infested with ***,*** and mixture of them were more effective in decreasing percentage of damping-off,root and pod rot disease incidence(%)in greenhouse and open field environment during the two seasons 2015 and *** by PGPR gave highly dry weight and number of healthy pods compared to control of fungi treatment which was nearby to dry weights of healthy pods achieved by treatments by Rizolex-T in a soil infested with ***,*** and mixture of *** PGPR *** B4 gave higher increasing of yield percentage than treatment by Rizolex-T in the two evaluated seasons 2015 and *** can conclude that the produced bioforumlated agent was more efficient as fungicide when compared with the other chemical synthesized fungicides,safe for human and the environment and economy.

关键词： peanut soil-borne diseases Bacillus subtilis biocontrol fermentation formulation

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