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PM_(2.5) obtained from urban areas in Beijing induces apoptosis by activating nuclear factor-kappa B

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Military Medical Research 2017年第4期4卷 200-209页

作者： Hui Peng Xiao-Hong Zhao Ting-Ting Bi Xiao-Yan Yuan Jia-Bin Guo Shuang-Qing Peng Evaluation and Research Center for Toxicology Institute of Disease Control and Prevention Academy of Military Medical Sciences Beijing key laboratory of bioactive substances and functional foods Beijing Union University

Background: Particulate matter(PM), which has adverse effects on citizen health, is a major air pollutant in Beijing city. PM_(2.5) is an indicator of PM in urban areas and can cause serious damage to human health. Many epidemiological studies have shown that nuclear factor-kappa B(NF-κB) is involved in PM_(2.5)-induced cell injury, but the exact mechanisms are not well ***: The cytotoxic effects of PM_(2.5) at 25–1600μg/ml for 24 h were determined by MTT assay in Chinese hamster ovary cells(CHO) cells. Flow cytometry was used to determine the apoptosis rate induced by PM_(2.5). The destabilized enhanced green fluorescent protein(d2 EGFP) green fluorescent protein reporter system was used to determine the NF-κB activity induced by PM_(2.5). The expression of pro-apoptotic Bcl-2-associated death promoter(BAD) proteins induced by PM_(2.5) was determined by Western blotting to explore the relationship between PM_(2.5) and the NF-κB signaling pathway and to determine the toxicological mechanisms of PM_(2.5).Results: PM_(2.5) collected in Beijing urban districts induces cytotoxic effects in CHO cells according to MTT assay with 72.28% cell viability rates even at 200μg/ml PM_(2.5) and flow cytometry assays with 26.97% apoptosis rates at 200μg/ml PM_(2.5). PM_(2.5) increases the activation levels of NF-κB, which have maintained for 24 h. 200μg/ml PM_(2.5) cause activation of NF-κB after exposure for 4 h, the activation peak appears after 13.5 h with a peak value of 25.41%. The average percentage of NF-κB activation in whole 24 h is up to 12.90% by 200μg/ml PM_(2.5). In addition, PM_(2.5) decreases the expression level of the pro-apoptotic protein BAD in a concentration-dependent ***: PM_(2.5) induces NF-κB activation, which persists for 24 h. The expression of pro-apoptotic protein BAD decreased with increased concentrations of PM_(2.5). These findings suggest that PM_(2.5) plays a major role in apoptosis by activating the NF-κB signaling path

关键词： Particulate matter NF-κB pathway Apoptosis BAD protein

Three new ursane-type triterpenoids from Rosmarinus officinalis and their biological activities

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Chinese Journal of Natural Medicines 2022年第2期20卷 155-160页

作者： ZHONG Xiang-Jian ZHOU Na WANG Xin LI Jin-Jie MA Hui JIAO Yue XU Jia-Hui LIN Peng-Cheng SHANG Xiao-Ya Beijing key laboratory of bioactive substances and functional foods Beijing Union UniversityBeijing 100191China Shandong Institute for Product Quality Inspection Jinan 250100China key laboratory for Qinghai-Tibet Plateau Phytochemistry of Qinghai Province Qinghai Nationalities UniversityXining 810000China

Three new ursane-type triterpenoids,3-oxours-12-en-20,28-olide(1),3β-hydroxyurs-12-en-20,28-olide(2)and 3β-hydroxyurs-11,13(18)-dien-20,28-olide(3),were isolated from a potent anti-inflammatory and antibacterial fraction of the ethanolic extract of Rosmarinus *** structures were elucidated by a combination of extensive 1D-and 2D-NMR experiments,MS data and comparisons with literature *** 1-3 exhibited significantly inhibitory effects on nitric oxide production in lipopolysaccharide-activated mouse RAW264.7 macrophages,but no antibacterial activity was found at a concentration of 128μg·mL^(-1).

关键词： Rosmarinus officinalis Isolation and purification Triterpenoid Biological activity

Fermented soy whey induced changes on intestinal microbiota and metabolic inﬂuence in mice

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Food Science and Human Wellness 2022年第1期11卷 41-48页

作者： Wei Han Xuhui Zhuang Qian Liu Bo Sun Haijiang Miao Xiaolin Zhang Academy of State Administration of Grain Beijing 100037China Beijing key laboratory of bioactive substances and functional foods Beijing Union UniversityBeijing 100191China COFCO Nutrition and Health Institute Beijing 102209China

Soy whey(SW)is generated as process waste while preparing soy protein isolates(SPI),and causing severe environmental ***,its value-added utilization is of prime importance for transforming and upgrading traditional *** study aims to utilize SW as a substrate for the growth of probiotics and produce a SW based *** a series of trials,the effect of the dietary supplementation with this fermented SW(FSW)was analyzed on ICR mice's body weight,metabolites,and intestinal microbiota in 4 *** results showed that,when SW was concentrated 15 times,the count of viable Lactobacillus casei reached 3.4×10^(9) CFU/mL by liquid fermentation method,which was the highest viable cell count among all test *** this FSW,the protein,amino acid,total dietary fibre,soluble dietary fibre,and oligosaccharide were 2.10%,1.63%,0.52%,0.51% and 0.79%,*** to two control group,the total yields of the short chain fatty acids(SCFAs)were significantly improved(75%-125% at average),while the SCFAs structure was also significantly changed(especially acetic acid and butyrate)in the faeces of mice fed ***,FSW dietary addition was associated with the diversity and richness of the intestinal ***,with mice's body weight loss,Firmicutes/Bacteroides ratio reduced accordingly(<1.21),and the abundance of Akkermansia muciniphila was significantly increased(the maximum amount was about 0.013%).In summary,our results indicated that the dietary supplementation of FSW affected mice's intestinal microbiota and metabolism and improved their health profile.

关键词： Soy whey Microbiota Short chain fatty acids Lactobacillus casei Akkermansia muciniphila

Magnetic Resonance Imaging of Alzheimer's Disease:from Diagnosis to Therapeutic Evaluation

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Chinese Journal of Integrative Medicine 2010年第3期16卷 276-282页

作者：顾雪梅姜招峰黄汉昌 Beijing Military General Hospital Beijing key laboratory of bioactive substances and functional foods Beijing Union University College of Arts and Science Beijing Union University

Alzheimer＇s disease（AD）is a devastating late-life dementia that produces progressive loss of memory and mental faculties in elderly *** is important to identify the earliest evidence of AD and to monitor the development of this disease for us to make positive response to its *** resonance imaging（MRI）is powerful to image the tissue or organ without *** can be employed to diagnose the early AD development and monitor the key biomarker development in *** may be helpful not only in diagnosing early AD,but also in evaluating its *** article reviews the progress of MRI on the diagnosis and detection of AD,and makes comments on its therapeutic application.

关键词： Alzheimer's disease diagnosis magnetic resonance imaging

Curcumin protects rat cortical neurons from Cu(Ⅱ)-Induced oxidative damage by the manner of coordination with Cu(Ⅱ) directly

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Curcumin protects rat cortical neurons from Cu(Ⅱ)-Induced o...

Mitochondrial dysfunction and cellularmetabolic deficiency in Alzheimer's disease

中国生物化学与分子生物学会第十届会员代表大会暨全国学术会议

作者： Hanchang Huang Zhaofeng Jiang Changjun Lin Beijing key laboratory of bioactive substances and functional foods Beijing Union University College of Arts and Science Beijing Union University

Background: A large number of evidence has suggested that Cu(II) is close relative to the Alzheimer’s disease *** level of serum Cu(II) in AD patients is about 115 percent of the normal aged people,and also the abnormal elevated Cu(II) has been detected at the core of and around the senile plaques,mainly deposited amyloid-B peptide(AB) in AD ***(II) on one hand may promote amyloid-B peptide to deposit in senile plaques,and on the other hand induce oxidative damage to neurons by the redox *** has

关键词： Alzheimer’s disease(AD) Coordination Copper Curcumin Oxidative Damage

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Neuroscience Bulletin 2012年第5期28卷 631-640页

作者： Xue-Mei Gu Han-Chang Huang Zhao-Feng Jiang Beijing Military General Hospital Beijing 100700 China Beijing key laboratory of bioactive substances and functional foods Beijing Union University Beijing 100191 China College of Life Science and Technology Beijing University of Chemical Technology Beijing 100029 China

Alzheimer＇s disease （AD） is an age-related neurodegenerative disorder. The pathology of AD includes amyloid-β （Aβ） deposits in neuritic plaques and neurofibrillary tangles composed of hyperphosphorylated tau, as well as neuronal loss in specific brain regions. Increasing epidemiological and functional neuroimaging evidence indicates that global and regional disruptions in brain metabolism are involved in the pathogenesis of this disease. Aβ precursor protein is cleaved to produce both extracellular and intracellular Aβ, accumulation of which might interfere with the homeostasis of cellular metabolism. Mitochondria are highly dynamic organelles that not only supply the main energy to the cell but also regulate apoptosis. Mitochondrial dysfunction might contribute to Aβ neurotoxicity. In this review, we summarize the pathways ofAβ generation and its potential neurotoxic effects on cellular metabolism and mitochondrial dysfunction.

关键词： Alzheimer’s disease amyloid-β metabolic deficiency mitochondrial dysfunction

Association Between Polymorphisms of DNA Repair Gene XRCC1 and DNA Damage in Asbestos-Exposed Workers

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Biomedical and Environmental Sciences 2006年第3期19卷 232-238页

作者： XIAO-HONG ZHAO CUANG JIA YONG-QUAN LIU SHAO-WEI LIU LEI YAN YU JIN NIAN LIU Beijing key laboratory of bioactive substances and functional foods Be（jing Union University Beijing 100083 China Department of Occupational and Environmental Health Sciences School of Public Health Peking University Beijing 100083 China Institue of Haidian Health Inspection and Supervision Beijing 100080 China

Objective To compare the asbestos-induced DNA damage and repair capacities of DNA damage between 104 asbestosexposed workers and 101 control workers in Qingdao City of China and to investigate the possible association between polymorphisms in codon 399 of XRCC1 and susceptibility to asbestosis. Methods DNA damage levels in peripheral blood lymphocytes were determined by comet assay, and XRCC1 genetic polymorphisms of DNA samples from 51 asbestosis cases and 53 non-asbestosis workers with a similar asbestos exposure history were analyzed by PCR/RFLP. Results The basal comet scores （3.95±2.95） were significantly higher in asbestos-exposed workers than in control workers （0.10±0.28）. After 1 h H2O2 stimulation, DNA damage of lymphocytes exhibited different increases. After a 4 h repair period, the comet scores were 50.98±19.53 in asbestos-exposed workers and 18.32±12.04 in controls. The residual DNA damage （RD） was significantly greater （P〈0.01） in asbestos-exposed workers （35.62%） than in controls （27.75%）. XRCC1 genetic polymorphism in 104 asbestos-exposed workers was not associated with increased risk of asbestosis. But compared with polymorphisms in the DNA repair gene XRCC1 （polymorphisms in codon 399） and the DNA damage induced by asbestos, the comet scores in asbestosis cases with Gin/Gin, Gln/Arg, and Arg/Arg were 40.26±18.94, 38.03±28.22, and 32.01±11.65, respectively, which were higher than those in non-asbestosis workers with the same genotypes （25.58±11.08, 37.08±14.74, and 29.38±10.15）. There were significant differences in the comet scores between asbestosis cases and non-asbestosis workers with Gin/Gin by Student＇s t-test （P〈0.05 or 0.01）. The comet scores were higher in asbestosis workers with Gin/Gin than in those with Arg/Arg and in non-asbestosis workers exposed to asbestos, but without statistically significant difference. Conclusions Exposure to asbestos may be related to DNA damage or the capacity of cells to repair H2O2-induced DNA damage. DNA re

关键词： Asbestos Asbestosis DNA damage XRCC 1 DNA repair Polymorphisms Comet assays

Calcineurin B prevents cardiac ischemia/reperfusion injury by up regulating expression of Bcl-2 family proteins

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Calcineurin B prevents cardiac ischemia/reperfusion injury b...

Isolation of exogenous fungi from Notoginseng Radix et Rhizoma and preliminary analysis of toxigenic fungi

第十届全国酶学学术讨论会

作者：郭俊霞 Shengquan Mi Jing Li Wei Liu Jinbo Cheng 魏群 Department of Biochemistry and Molecular Biology Beijing Key Laboratory of Genetic Engineering Medicine and BiotechnologyBeijing Normal University Beijing key laboratory of bioactive substances and functional foods College of Arts and Science of Beijing Union University

Calcineurin B(CnB) is the regulatory subunit of calcineurin(Cn),a Ca/ calmodulin-dependent serine/threonine protein *** suggests that increased CnB expression decreases ischemia/reperfusion injury to the heart,and decreasing calcineurin activity increases susceptibility to cardiac *** hypothesized that pretreatment with CriB might suffice to activate calcineurin and protect cardiomyocytes during subsequent ischemia/reperfusion *** isolated perfused rat hearts,CnB improved

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Journal of Future foods 2024年第2期4卷 179-182页

作者： Lei Zhou Xiangsheng Zhao Xiaomin Liu Hefang Wan Chun Sui Xuyu Chen key laboratory of bioactive substances and Resources Utilization of Chinese Herbal Medicine Ministry of EducationNational Engineering Laboratory for Breeding of Endangered Medicinal MaterialsInstitute of Medicinal Plant Development(IMPLAD)Chinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100193China Hainan Provincial key laboratory of Resources Conservation and Development of Southern Medicine Hainan Branch of the Institute of Medicinal Plant DevelopmentChinese Academy of Medical Sciences&Peking Union Medical CollegeHaikou 570311China

Notoginseng Radix et Rhizoma(Sanqi in Chinese)is a precious traditional Chinese herbal *** has the effect of dispersing blood stasis and stopping bleeding,reducing swelling and fixing ***,it tends to contaminate with harmful fungi during storage,which may make it much less *** order to understand the fungal contamination of Notoginseng Radix et Rhizoma and master its composition of the exogenous *** surface fungi of Notoginseng Radix et Rhizoma samples collected from six Chinese provinces and districts were investigated by using dilution plate *** of aflatoxins by UPLC-MS/*** results showed that Penicillium citrinum was dominantly isolated from Notoginseng Radix et Rhizoma samples from No.1 to *** flavus,which produces aflatoxin,was dominantly isolated from Notoginseng Radix et Rhizoma samples from No.5 and *** addition,kinds of mycotoxin were assayed which were produced by three of those identified *** three fungi strains produced aflatoxin B1(AFB1)and one strain HBSQ1-5 additionally produced other three kinds of mycotoxin,AFB2,AFG1 and *** is the results implied that it will be very important to take serious cautions when using Notoginseng Radix et *** well as,understanding the composition of the exogenous fungi of Notoginseng Radix et Rhizoma and the strains of toxin-producing fungi,which can play an important role in guiding the storage of Notoginseng Radix et Rhizoma.

关键词： Notoginseng Radix et Rhizoma Exogenous fungal Amplicon sequencing Mycotoxin