检索结果-南通市图书馆

Microbial Activity Indices: Sensitive Soil Quality Indicators for Trace Metal Stress

在线全文

学校读者我要写书评

暂无评论

Pedosphere 2005年第4期15卷 409-416页

作者： LI Yong-Tao T. BECQUER2 C. QUANTIN M. BENEDETTI P. LAVELLE DAI Jun College of Natural Resources and Environment South China Agricutrual University Guangzhou 510642 （China） UMR 137 Biodiversité et Fonctionnement des Sols Institut de Recherche Pour le Déueloppement （France） Université Paris Sud XI FRE Orsay Terre 2566 bat 504 91405 Cedex （France） UMR-CNRS 7047 Lab. de Géochimie et Métallogénie/Université Pierre et Marie Curie （France）

Physicochemical properties, total and DTPA （diethylenetriaminepentaacetic acid）-extractable Cu, Zn, Pb and Cd contents, microbial biomass carbon （C） content and the organic C mineralization rate of the soils in a long-term trace metal-contaminated paddy region of Guangdong, China were determined to assess the sensitivity of microbial indices to moderately metal-contaminated paddy soils. The mean contents of total Cu, Zn, Pb and Cd were 251, 250, 171, and 2.4 mg kg^-1 respectively. DTPA-extractable metals were correlated positively and significantly with total metals, CEC, and organic C （except for DTPA-extractable Cd）, while they were negatively and highly significantly correlated with pH, totall Fe and Mn. Metal stress resulted in relatively low ratios of microbial biomass C to organic C and in remarkable inhibition of the microbial metabolic quotient and C mineralization rate, which eventually led to increases in soil organic C and C/N. Moreover, microbial respiratory activity showed a stronger correlation to DTPA-extractable metals than to total metal content. Likewise, in the acid paddy soils some “linked” microbial activity indices, such as metabolic quotient and ratios of basal respiration to organic C, especially during initial incubation, were found to be more sensitive indicators of soil trace metal contamination than microbial biomass C or basal respiration alone.

关键词： availability indication microbial characteristics trace metals

The Cellular Populations of Normal Camel (<i>Camelus dromedaries</i>) Milk

在线全文

学校读者我要写书评

暂无评论

Open Journal of Veterinary Medicine 2012年第4期2卷 262-265页

作者： Kazem T. Al-Mohammed Salem Fateh A. Badi Amal I. Al Haroon Ahmed M. Alluwaim Animal Quarantine Ministry of Agriculture Al Bat’ha Saudi Arabia Department of Microbiology and Parasitology College of Veterinary Medicine King Faisal Al Ahsaa Saudi Arabia Department of Microbiology College of Science King Saud University Riyadh Saudi Arabia Immunology Unit Regional diagnostic laboratory Ministry of Health Dammam Saudi Arabia

Overwhelming demand on the camel milk has considered camel mammary glands as an important organ. However, poor research of the camel mammary glands immune system is considered major obstacle in improving the camel welfare. The cellular population of camel milk at the mid-lactation was exploited using overlapping reactive antibodies. The CD markers and adhesion molecules, CD3+, CD8+, WC + 1+, CD62L, CD11b/c (MAC-1)and the LPAM-1 were studied with flow cytometer. The high expression of CD3+, CD8+, WC+1+ and LPAM-1 was detected in all of the examined samples. The CD62L, CD11b/c expression were not detected consistently. The cross reacted antibodies with camel CD markers have revealed interesting overview of the nature of the cellular activities in the camel mammary glands at the lactation period. The level of CD8+ cells is in parallel with the findings at the cattle mammary glands. The high level of WC + 1+ γδ cells in camel milk, despite the stage of the lactation and age, could indicate their significant role in the immunity of the camel mammary glands. The expression of the LPAM-1 on the lymphocytes has provided further support to the notion that the lymphocytes trafficking to the camel mammary glands could be of mucosal nature.

关键词： Camel Mammary Glands Wc1 Cd8 Lymphocyte Trafficking Lactation

A highly potent and stable pan-coronavirus fusion inhibitor as a candidate prophylactic and therapeutic for COVID-19 and other coronavirus diseases

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Acta Pharmaceutica Sinica B 2022年第4期12卷 1652-1661页

作者： Jie Zhou Wei Xu Zezhong Liu Chao Wang Shuai Xia Qiaoshuai Lan Yanxing Cai Shan Su Jing Pu Lixiao Xing Youhua Xie Lu Lu Shibo Jiang Qian Wang Key laboratory of Medical Molecular Virology(MOE/NHC/CAMS) School of Basic Medical Sciences and BSL-3 FacilityShanghai Institute of Infectious Diseases and BiosecurityFudan UniversityShanghai 200032China State Key laboratory of Toxicology and Medical Countermeasures Beijing Institute of Pharmacology and ToxicologyBeijing 100850China

The development of broad-spectrum antivirals against human coronaviruses(HCoVs)is critical to combat the current coronavirus disease 2019(COVID-19)pandemic caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)and its variants,as well as future outbreaks of emerging *** have previously identified a polyethylene glycol-conjugated(PEGylated)lipopeptide,EK1C4,with potent pan-CoV fusion inhibitory ***,PEG linkers in peptide or protein drugs may reduce stability or induce anti-PEG antibodies in ***,we herein report the design and synthesis of a series of dePEGylated lipopeptide-based pan-CoV fusion inhibitors featuring the replacement of the PEG linker with amino acids in the heptad repeat 2 C-terminal fragment(HR2-CF)of *** these lipopeptides,EKL1C showed the most potent inhibitory activity against infection by SARS-CoV-2 and its spike(S)mutants,as well as other HCoVs and some bat SARS-related coronaviruses(SARSr-CoVs)*** dePEGylated lipopeptide EKL1C exhibited significantly stronger resistance to proteolytic enzymes,better metabolic stability in mouse serum,higher thermostability than the PEGylated lipopeptide EK1C4,suggesting that EKL1C could be further developed as a candidate prophylactic and therapeutic for COVID-19 and other coronavirus diseases.

关键词： Coronavirus Lipopeptide SARS-CoV-2 Polyethylene glycol Fusion inhibitor

Discovery and characterization of novel paramyxoviruses from bat samples in China

在线全文

学校读者我要写书评

暂无评论

Virologica Sinica 2023年第2期38卷 198-207页

作者： Haoxiang Su Yuyang Wang Yelin Han Qi Jin Fan Yang Zhiqiang Wu NHC Key laboratory of Systems Biology of Pathogens Institute of Pathogen BiologyChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing100730China Key laboratory of Respiratory Disease Pathogenomics Chinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing100730China

Many paramyxoviruses are responsible for a variety of mild to severe human and animal *** on the novel discoveries over the past several decades,the family Paramyxoviridae infecting various hosts across the world includes 4 subfamilies,17 classified genera and 78 species ***,no systematic surveys of bat paramyxoviruses are available from the Chinese *** this study,13,064 samples from 54 bat species were collected and a comprehensive paramyxovirus survey was *** obtained 94 new genome sequences distributed across paramyxoviruses from 22 bat species in seven *** phylodynamic and phylogenetic analyses showed that there were four different lineages in the Jeilongvirus *** on available data,results of host and region switches showed that the bat colony was partial to interior,whereas the rodent colony was exported,and the felines and hedgehogs were most likely the intermediate hosts from Scotophilus *** than *** on the evolutionary trend,genus Jeilongvirus may have originated from Mus *** Australia,then transmitted to bats and rodents in Africa,Asia and Europe,and finally to bats and rodents in America.

关键词： Paramyxovirus Jeilongvirus bat Host and region switches China

Evaluation of a Rapid Immunochromatographic Middle East Respiratory Syndrome Coronavirus Antigen Detection Assay

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Infectious Microbes & Diseases 2022年第4期4卷 175-177页

作者： Susanna Kar-Pui Lau Kenneth Sze-Ming Li Jade Lee-Lee Teng Sunitha Joseph Hayes Kam-Hei Luk Joshua Fung Ulrich Wernery Patrick Chiu-Yat Woo Department of Microbiology School of Clinical MedicineLi Ka Shing Faculty of MedicineThe University of Hong KongPokfulamHong KongChina Central Veterinary research laboratory DubaiUnited Arab Emirates PhD Program in Translational Medicine and Department of Life Sciences National Chung Hsing UniversityTaichungTaiwan The iEGG and Animal Biotechnology research Center National Chung Hsing UniversityTaichungTaiwan

Middle East respiratory syndrome coronavirus(MERS-CoV)infection in humans has a high mortality of>30%.Dromedaries are the reservoir of MERS-CoV and the main source of human ***,MERS-CoV infections in dromedaries are usually *** diagnosis of MERS-CoV infection in these animals is important in preventing camel-to-human transmission of the *** possible cross-reactivity of a previously reported rapid nucleocapsid protein-based antigen detection assay for MERS-CoV was examined with different CoVs,including Tylonycteris bat CoV HKU4,dromedary camel CoV UAE-HKU23,human CoV-229E,human CoV-OC43,severe acute respiratory syndrome CoV-2 and rabbit CoV HKU14,where none of them showed false-positive *** assay was further validated using quantitative real-time reverse transcription-polymerase chain reaction-confirmed MERS-CoV-positive and MERS-CoV-negative dromedary nasal samples collected in Dubai,the United Arab Emirates,which showed that the rapid antigen detection assay has a specificity of 100%and sensitivity of 91.7%.

关键词： MERS-CoV rapid antigen detection assay dromedary camels

TRADE-OFFS IN THE DESIGN OF SUSTAINABLE CROPPING SYSTEMS AT A REGIONAL LEVEL:A CASE STUDY ON THE NORTH CHINA PLAIN

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Frontiers of Agricultural Science and Engineering 2022年第2期9卷 295-308页

作者： Jeroen C.J.GROOT Xiaolin YANG Farming Systems Ecology Wageningen University&ResearchP.O.Box 4306700 AK Wageningenthe Netherlands International Maize and Wheat Improvement Center(CIMMYT) Sustainable Intensification ProgramCarretera México-VeracruzKm.45El Batán56237 TexcocoMéxico Alliance of Bioversity International and CIAT PerformanceInnovation and Strategic Analysis for Impact unitVia di San Domenico 100153 RomeItaly College of Water Resources&Civil Engineering China Agricultural UniversityBeijing 100193China

Since the Green Revolution cropping systems have been progressively homogenized and intensified with increasing rates of inputs such as fertilizers,pesticides and *** has resulted in higher crop productivity but also a high environmental burden due to increased pollution and water *** identify opportunities for increasing the productivity and reducing the environmental impact of cropping systems,it is crucial to assess the associated *** paper presents a model-based analysis of how 30 different crop rotations practiced in the North China Plain could be combined at the regional level to overcome trade-offs between indicators of economic,food security,and environmental *** model uses evolutionary multi-objective optimization to maximize revenues,livestock products,dietary and vitamin C yield,and to minimize the decline of the groundwater *** modeling revealed substantial trade-offs between objectives of maximizing productivity and profitability versus minimizing ground water decline,and between production of livestock products and vitamin C *** strategies each defining a specific combination of cropping systems and contributing to different extents to the various objectives were *** of these six strategies could be used to find opportunities to mitigate the tradeoffs between *** was concluded that a holistic analysis of the potential of a diversity cropping systems at a regional level is needed to find integrative solutions for challenges due to conflicting objectives for food production,economic viability and environmental protection.

关键词： crop rotation food security multi-objective optimization water use

The persistent prevalence and evolution of cross-family recombinant coronavirus GCCDC1 among a bat population:a two-year follow-up

在线全文

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2017年第12期60卷 1357-1363页

作者： Joseph O.Obameso Hong Li Hao Jia Min Han Shiyan Zhu Canping Huang Yuhui Zhao Min Zhao Yu Bai Fei Yuan Honglan Zhao Xia Peng Wen Xu Wenjie Tan Yingze Zhao Kwok-Yung Yuen William J.Liu Lin Lu George F.Gao CAS Key laboratory of Pathogenic Microbiology and Immunology Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China Yunnan Provincial Center for Disease Control and Prevention Kunming 650022China University of Chinese Academy of Sciences Beijing 100049China College of laboratory Medicine and Life Sciences Wenzhou Medical UniversityWenzhou 325035China Key laboratory of Medical Virology and Viral Diseases Ministry of Health of People's Republic of ChinaNational Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China State Key laboratory of Emerging Infectious Diseases The University of Hong KongHong Kong Special Administrative RegionHong KongChina

bats are connected with the increasing numbers of emerging and re-emerging viruses that may break the species barrier and spread into the human population. Coronaviruses are one of the most common viruses discovered in bats, which were considered as the natural source of recent human-susceptible coronaviruses, i.e. SARS-COV and MERS-CoV. Our previous study reported the discovery of a bat-derived putative cross-family recombinant coronavirus with a reovirus gene p10, named as Ro-batCoV GCCDC1. In this report, through a two-year follow-up of a special bat population in one specific cave of south China, we illustrate that Ro-batCoV GCCDC1 persistently circulates among bats. Notably, through the longitudinal observation, we identified the dynamic evolution of Ro-batCoV GCCDC1 in bats represented by continuously recombination events. Our study provides the first glimpse of the virus evolution in one longitudinally observed bat population cohort and underlines the surveillance and pre-warning of potential interspecies transmittable viruses in bats.

关键词： coronavirus bat population evolution

HLA-B-associated transcript 3 (bat3) stabilizes and activates p53 in a HAUSP-dependent manner

在线全文

学校读者我要写书评

暂无评论

Journal of Molecular Cell Biology 2020年第2期12卷 99-112页

作者： Rui Zhang Di Cui TengXue Yue Lang Yunfan Zhang Lianjie Li Haili Sun Yu Kuang Gebin Li Jun Tang Department of Basic Veterinary College of Veterinary MedicineChina Agricultural UniversityBeijing 100193China State Key laboratory of Agrobiotechnology China Agricultural UniversityBeijing 100193China Department of Clinical Veterinary College of Veterinary MedicineChina Agricultural UniversityBeijing 100193China Clinical research Institute Zhejiang Provincial People's HospitalPeople's Hospital of Hangzhou Medical CollegeHangzhou 310014China

The p53 pathway is a highly complex signaling network including several key *** is a critical component of the p53 pathway acting as a deubiquitinase for both p53 and its key repressor ***,we identified a novel HAUSP-interacting protein,HLA-B-associated transcript 3(bat3)and found it to be capable of inducing p53 stabilization and activation via a HAUSP-dependent mechanism,resulting in cell growth ***,the deubiquitylating enzymatic activity of HAUSP was not required for this ***-immunoprecipitation showed that p53 coexisted in a complex with bat3 and HAUSP in vivo,and HAUSP may serve as a binding mediator to enhance the interaction between p53 and *** studies revealed that formation of this three-protein complex interfered with the binding of p53 to its proteasome receptor S5a and promoted the accumulation of p53 in ***,Mdm2 protein abundance is also regulated by bat3 in the presence of *** of bat3 and HAUSP increases Mdm2 protein levels without influencing the p53–Mdm2 interaction and Mdm2-mediated p53 ubiquitination,indicating that bat3–HAUSP-mediated protein stabilization is not specific to p53 and different mechanisms may be involved in bat3-mediated regulation of p53–Mdm2 ***,our study unravels a novel mechanism by which p53 is stabilized and activated by HAUSP-mediated interaction with bat3 and implies that bat3 might function as a tumor suppressor through the stabilization of p53.

关键词： bat3 p53 stabilization HAUSP S5a proteasome recognition

Myotis bat STING attenuates aging-related inflammation in female mice

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Zoological research 2024年第5期45卷 961-971页

作者： Xi Wang Jing-Kun Jia Qi Wang Jing-Wen Gong Ang Li Jia Su Peng Zhou Wuhan Institute of Virology Chinese Academy of SciencesWuhanHubei 430071China Guangzhou National laboratory Guangzhou International Bio IslandGuangzhouGuangdong 510005China University of Chinese Academy of Sciences Beijing 100000China State Key laboratory of Respiratory Disease First Affiliated Hospital of Guangzhou Medical SchoolGuangzhouGuangdong 510005China

bats,notable as the only flying mammals,serve as natural reservoir hosts for various highly pathogenic viruses in humans(e.g.,SARS-CoV and Ebola virus).Furthermore,bats exhibit an unparalleled longevity among mammals relative to their size,particularly the Myotis bats,which can live up to 40 ***,the mechanisms underlying these distinctive traits remain incompletely *** our prior research,we demonstrated that bats exhibit dampened STING-interferon activation,potentially conferring upon them the capacity to mitigate virus-or aging-induced *** substantiate this hypothesis,we established the first in vivo bat-mouse model for aging studies by integrating Myotis davidii bat STING(MdSTING)into the mouse *** monitored the genotypes of these mice and performed a longitudinal comparative transcriptomic analysis on MdSTING and wild-type mice over a 3-year aging *** transcriptomic analysis indicated a reduction in aging-related inflammation in female MdSTING mice,as evidenced by significantly lower levels of pro-inflammatory cytokines and chemokines,immunopathology,and neutrophil recruitment in aged female MdSTING mice compared to aged wild-type mice in *** results indicated that MdSTING knock-in attenuates the aging-related inflammatory response and may also improve the healthspan in mice in a sex-dependent *** the underlying mechanism awaits further study,this research has critical implications for bat longevity research,potentially contributing to our comprehension of healthy aging in humans.

关键词： bat STING Longevity Aging-related inflammation Virus reservoir host