检索结果-南通市图书馆

Compressed wormlike chain moving out of confined space: A model of DNA ejection from bacteriophage

在线全文

学校读者我要写书评

暂无评论

Acta Mechanica Sinica 2012年第4期28卷 1219-1226页

作者： Ji-Zeng Wang Long Li Hua-Jian Gao Key laboratory of Mechanics on Disaster and Environment in Western China Ministry of EducationSchool of Civil Engineering and MechanicsLanzhou UniversityLanzhou730000 GansuChina School of Engineering Brown UniversityProvidence02912 Rhode IslandUSA

The molecular biomechanics of DNA ejection from bacteriophage is of interest to not only fundamental biological understandings but also practical applications such as the design of advanced site-specific and controllable drug delivery systems. In this paper, we analyze the viscous motion of a semiflexible polymer chain coming out of a strongly confined space as a model to investigate the effects of various structure confinements and frictional resistances encountered during the DNA ejection process. The theoretically predicted relations between the ejection speed, ejection time, ejection length, and other physical parameters, such as the phage type, total genome length and ionic state of external buffer solutions, show excellent agreement with in vitro experimental observations in the literature.

关键词： DNA ejection bacteriophage. Wormlike chain model Confined semi-flexible polymer. Drag coefficient

Complete genome sequence and proteomic analysis of a thermophilic bacteriophage BV1

在线全文

学校读者我要写书评

暂无评论

Acta Oceanologica Sinica 2010年第3期29卷 84-89页

作者： LIU Bin WU Suijie XIE Lianhui Institute of Plant Virology Fujian Agriculture and Forestry University Fuzhou 350002 China College of Food Science Fujian Agriculture and Forestry University Fuzhou 350002 China Key laboratory of Marine Biogenetic Resources Third Institute of Oceanography State Oceanic Administration Xiamen 361005 China

Viruses of thermophiles are of great interest due to their roles in gene transfer, global geochemical cycle and evolution of life on earth. However, the thermophilic bacteriophages have not been studied extensively. In this investigation, a typical bacteriophage BV1 was obtained from a thermophilic bacterium Geobacillus sp. 6k512, which was isolated from an inshore hot spring in Xiamen of China. The BV1 contained a double-stranded linear DNA of 35 055 bp, which encodes 54 open reading frames （ORFs）. Interestingly, eight of the 54 BV1 ORFs shared sequence similarities to genes from human disease-relevant bacteria. Seven proteins of the purified BV1 virions were identified by proteomic analysis. Determination of BV1 functional genomics would facilitate the better understanding of the mechanism for virus-thermophile interaction.

关键词： thermophile bacteriophage genome sequence proteomic analysis

Bifunctional Phage Cocktail for Multiplexed Detection of Pathogens

在线全文

学校读者我要写书评

暂无评论

Bifunctional Phage Cocktail for Multiplexed Detection of Pat...

高度稀释的顺势疗法药物对噬菌体感染的细菌基因水平的作用(英文)

第十一届全国化学生物学学术会议

作者： Xinyi Hong Tian Luan Liuqing Su Tingting Huang Lina Wu Xiaomei Yan MOE Key laboratory of Spectrochemical Analysis ＆ Instrumentation Key Laboratory for Chemical Biology of Fujian ProvinceDepartment of Chemical BiologyCollege of Chemistry and Chemical EngineeringXiamen University

Foodborne diseases represent a serious threat to public health,and rapid detection of foodborne pathogens is essential to the food safety *** are bacterial viruses that specifically infect their host cells and have been used as recognition elements in developing biosensors for *** merits of phages include the low cost and ease of *** the advantage that phage capsid is composed of several capsid proteins,here we report the generation of bifunctional phage "reagents" for bacterial *** phage M13 is a filamentous bacteriophage composed of circular single stranded DNA（ssDNA） encapsulated in approximately 2700 copies of the major coat protein PVIII,and capped with five copies of each of proteins PVII and PXI in one end,and five copies of each of proteins PVI and PIII on the *** genetic enineering,we derived a M13 phage to allow it display targeting ligand and avidin specific peptide,on PIII and PVIII proteins,*** that the engineered phage is bifunctional of both target binding and signal *** kinds of bifunctional phages aimed at the detection of *** O157:H7,Salmonella typhimurium,and Pseudomonas aeruginosa,respectively have been *** experimental results demonstrated that these bifunctional phages can infect their native host cells efficiently and *** cross recognition of non-target bacteria was ***,using the mixture of bifunctional phages as a cocktail,we achieved simultaneous detection of multiple bacterial pathogens of different *** application of bifunctional phages in the pathogen detection of food and environmental samples is expected.

关键词： Bifunctional phage multiplex bacterial detection flow cytometry

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

中西医结合学报 2012年第4期10卷 462-470页

作者： Santu Kumar Saha Sreemanti Das Anisur Rahman Khuda-Bukhsh Cytogenetics and Molecular Biology laboratory Department of ZoologyUniversity of KalyaniKalyani 741235India

目的:探讨具有抗病毒作用的高度稀释的顺势疗法药物对噬菌体感染的大肠杆菌在基因水平调节噬菌体ΦX174 DNA的作用。方法:本研究之所以选用噬菌体ΦX174是因为其对大肠杆菌的宿主特异性及其在宿主内进行溶菌素基因E的组成性表达。采用... 详细信息

目的:探讨具有抗病毒作用的高度稀释的顺势疗法药物对噬菌体感染的大肠杆菌在基因水平调节噬菌体ΦX174 DNA的作用。方法:本研究之所以选用噬菌体ΦX174是因为其对大肠杆菌的宿主特异性及其在宿主内进行溶菌素基因E的组成性表达。采用顶层琼脂法,计数琼脂板上的斑块数量以衡量不同的顺势疗法药物对噬菌体感染的大肠杆菌的保护作用。被噬菌体感染的大肠杆菌接受不同顺势疗法药物的干预,以高度稀释的乙醇做为安慰剂对照,并加设空白对照组。琼脂板上的斑块数量表明菌群被噬菌体ΦX174感染并溶解的数量。反之,我们在用顺势疗法药物干预前将噬菌体ΦX174混入药物中,再与细菌作用,以确定药物本身对感染细菌的噬菌体ΦX174没有作用。结果:每一种顺势疗法药物干预后的细菌琼脂板上的斑块数量均较安慰剂对照组和空白对照组有显著下降;而混入药物的噬菌体ΦX174感染细菌后,琼脂板上的斑块数量并无明显下降。因为噬菌体ΦX174在细菌内开始其溶菌过程,斑块数量的下降可能是因为溶菌素基因E被抑制或者整个噬菌体ΦX174的DNA被大肠杆菌内的基因产物(抑制酶)所破坏。结论:本研究的结果证实了高度稀释的顺势疗法药物对噬菌体感染的大肠杆菌在基因水平有调节作用。

关键词：细菌噬菌体φX174 大肠杆菌顺势疗法基因表达调控菌斑试验

A<i>β</i>-Like Peptide Displayed on bacteriophage T7 Catalyzes Chromate and Uranyl Reduction

在线全文

学校读者我要写书评

暂无评论

Journal of Environmental Protection 2013年第8期4卷 857-868页

作者： Hongjun Jin Chiann-Tso Lin Jianying Shang Michael J. Wilkins Yingbin Liu Wei Gong Weiping Xu Thomas C. Squier Philip E. Long 1Fundamental & Computational Sciences Directorate Pacific Northwest National Laboratory Richland USA 2Department of Radi-ological Science Washington University School of Medicine St. Louis USA Earth Sciences Division Lawrence Berkeley National Laboratory Berkeley USA Fundamental & Computational Sciences Directorate Pacific Northwest National Laboratory Richland USA Xinhua Hospital Affiliated to Shanghai Jiaotong University Shanghai China

In order to discover genes capable of catalyzing the reductive immobilization of toxic chromate and uranyl ions, we have created a T7 bacteriophage library containing cDNA from environmental microbes (i.e., Geobacter sulfurreducens and Shewanella oneidensis MR-1) that are known to mediate the reduction of chromate and uranyl ions. After three rounds of screening, ten bacteriophage mutants were found to mediate the NADH-dependent reduction of chromate and uranyl ions whose cDNA encodes polypeptide chains ranging 14 to 73 amino acids in length. All identified sequences contain disordered structural motifs similar to the β-amyloid peptide (Aβ) known to promote aggregation and formation of high-affinity metal binding sites. Confirmation of this structural similarity involved phage display of the 42 amino-acid Aβ-peptides that have been found to catalyze the NADH-dependent reduction of both chromate and uranyl ions. Transmission electron microscopy (TEM) and X-ray absorption near edge structure (XANES) measurements confirm that reduced uranium is present on the surface of bacteriophage expressing the Aβ-peptide. The surface-displayed Aβ-like peptide on bacteriophage has the potential to couple naturally occurring electron transfer shuttles present in soils to promote economically viable remediation of contaminated sites containing toxic chromate and uranyl ions.

关键词： Chromate Uranyl Bioremediation bacteriophage

bacteriophage Endolysins:the Bacteria Killer from Nature

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Bacteriophage Endolysins:the Bacteria Killer from Nature

Choice of Force Fields and Water Models for Sampling Solution Conformations of bacteriophage T4 Lysozyme

2012年鄂粤微生物学学术年会——湖北省暨武汉微生物学会成立六十年庆祝大会

作者： Hang Yang Dian-Bing Wang Qiuhua Dong Zhiping Zhang Zongqiang Cui Jiaoyu Deng Junping Yu Xian-en Zhang Hongping Wei State Key laboratory of Virology Wuhan Institute of VirologyChinese Academy of Sciences

Bacterial resistance is one of the greatest therapeutic challenges worldwide. Significantly,the emergence of super bacteria and Methicillin-resistant Staphylococcus aureus(MRSA) raise serious concerns within the medical community. Exploiting new antibiotics is the most classic strategy that has been widely used to encounter bacterial ***,this strategy has become much more difficult in an age of multidrug-resistant pathogens emerging,and suffers from its *** circumvent these drawbacks,the bacteriophage lysin evolving synchronically with bacteria and targeting the essential element for bacterial viability appears to be particularly *** are expressed in the later phase of double-stranded DNA phages infection to hydrolyze the bacterial cell wall for the release of progeny *** applied exogenously,lysins are able to cleave the integral peptidoglycan bonds of susceptible Gram-positive pathogenic bacteria and result in a rapid cell ***,it has displayed outstanding advantages over other antibiotics attributing to its rapid antibacterial activity and narrow lytic *** importantly,bacteria harbor a very low probability of developing resistance to lysins for its strong binding affinity and essential targets. Herein,plyG has been identified to be the first lysin that with separate domain for recognizing spores and vegetative cells.A 60 amino acid domain in plyG,was found able to specifically recognize *** spores,but not vegetative *** exosporium of the spores was found to be the most probable binding *** is the first time that a lysin for spore-forming bacteria has been found to have separate domains to recognize spores and vegetative cells,which might help to understand the co-evolution of phages with spore-forming *** providing new biomarkers for developing better assays for identifying *** spores,the newly found domain may be helpful in developing plyG as a prevent

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Chinese Journal of Chemical Physics 2021年第4期34卷 487-496,I0004-I0010页

作者： Xin-zheng Du Xin-fan Hua Zhi-yong Zhang MOE Key laboratory for Membraneless&Cellular Dynamics National Science Center for Physical Sciences at the MicroscaleDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230026China

A protein may exist as an ensem-ble of di erent conformations in solution,which cannot be repre-sented by a single static *** dy-namics(MD)simulation has become a useful tool for sampling protein conformations in solution,but force elds and water models are important *** work presents a case study of the bacteriophage T4 lysozyme(T4L).We have found that MD simulations using a classic AMBER99SB force eld and TIP4P water model cannot well describe hinge-bending domain motion of the wild-type T4L at the timescale of one *** combinations,such as a residue-speci c force eld called RSFF2+and a dispersion-corrected water model TIP4P-D,are able to sample reasonable solution conformations of T4L,which are in good agreement with experimental *** primary study may provide candidates of force elds and water models for further investigating conformational transition of T4L.

关键词： Molecular dynamics simulation Force eld Water model Domain motion bacteriophage T4 lysozyme

Research Progress in Phage

在线全文

学校读者我要写书评

暂无评论

Agricultural Science & Technology 2016年第7期17卷 1709-1713页

作者： Mingxu XIE Shigen YE Xiaoyu YANG Key laboratory of Mariculture & Stock Enhancement in Northern China's Sea Ministry of AgricultureDalian Ocean University

With excessive utilization of antibiotics in recent years,bacterial drug resistance problem is serious increasingly,and it is more and more difficult to develop anti-infective drug,while it does not have these problems to use phage controlling *** is a kind of prokaryotic virus,widely exists in the nature and includes bacteriophage,cyanophage and *** to its potential of replacing antibiotics to treat disease,phage receives more and more *** this paper,based on development status of phage research at home and abroad,discovery process,naming method and classification basis of phage are introduced comprehensively,and advantages and limitations of phage applying in prevention and control of bacterial diseases are *** introduce application status of phage in human medicine,prevention and control of diseases for terrestrial animals and aquaculture,and the effects of phage in sewage treatment,prevention and control of microbial contamination of food and detection technology,and point out the shortages of phage in the above ***,we also discuss application prospects of phage in disease prevention and control,environmental protection and food safety.

关键词： Phage Disease prevention and control Advantage Limitation Progress Application

Cellular responses in Bacillus thuringiensis CS33 during bacteriophage BtCS33 infection

在线全文

学校读者我要写书评

暂无评论

Cellular responses in Bacillus thuringiensis CS33 during bac...