Genomic compositions and phylogenetic analysis of Shigella boydii subgroup

作     者：WANG Jing ZHANG Xiaobing PENG Junping YANG E BIN Wen YANG Jian DONG Jie SUN Lilian XU Xingye JIN Qi 

作者机构：State Key Laboratory for Molecular Virology and Genetic EngineeringBeijing 100176China School of MedicineXi’an Jiaotong UniversityXi’an 710061China School of Pharmaceutical EngineeringShengyang Pharmaceutical UniversityShengyang 110016China 

出 版 物：《Science China(Life Sciences)》 (中国科学(生命科学英文版))

年 卷 期：2006年第49卷第1期

页      面：46-52页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 07[理学] 071005[理学-微生物学] 10[医学] 

基　　金：International Science and Technology Co-operation Project, (2001AA223116) National High-tech Research and Development Program, (2001AA223011) National Key Research and Development Program of China, NKRDPC, (G1999054103) 

主　　题：S.boydii comparative genomic hybridization(CGH) strain-specific ORFs evolution 

摘      要：Comparative Genomic Hybridization (CGH) microarray analysis was used tocompare the genomic compositions of all eighteen Shigella boydii serotype representative *** results indicated the genomic backbone of this subgroup contained 2552 ORFs homologous tononpatho-genic E. coli K12. Compared with the genome of K12199 ORFs were found to be absent in allS. boydii serotype representatives, including mainly outer membrane protein genes and O-antigenbiosynthesis genes. Yet the specific ORFs of S. boydii subgroup contained basically bacteriophagegenes and the function unknown (FUN) genes. Some iron metabolism, transport and type Ⅱ secretionsystem related genes were found in most representative strains. According to the CGH phylogeneticanalysis, the eighteen S. boydii serotype representatives were divided into four groups, in whichserotype C13 strain was remarkably distinguished from the other serotype strains. This groupingresult corresponded to the distribution of some metabolism related genes. Furthermore, the analysisof genome backbone genes, specific genes, and the phylogenetic trees allowed us to discover theevolution laws of S. boydii and to find out important clues to pathogenesis research, vaccinationand the therapeutic medicine development.