检索结果-南通市图书馆

Cloning,expression in Escherichia coli,and enzymatic properties of laccase from Aeromonas hydrophila WL-11

在线全文

学校读者我要写书评

暂无评论

Journal of Environmental Sciences 2010年第4期22卷 635-640页

作者： Jing Wu Kyoung-Sook Kim Jai-Heon Lee Young-Choon Lee Department of biotechnology Dong-A University Busan 604-714 South Korea. Brain Korea 21 center for silver-bio industrialization Dong-A University Busan 604-714 South Korea

A strain WL-11 with high laccase activity was isolated from activated sludge collected from the effluent treatment plant of a textile and dyeing industry. It was identified as Aeromonas hydrophila by physiological test and 16S rDNA sequence analysis. A gene encoding of laccase from a newly isolated Aeromonas hydrophila WL-11 was cloned and characterized. Nucleotide sequence analysis showed an open reading frame of 1605 bp encoding a polypeptide comprised of 534 amino acids. The primary structure of the enzyme predicted the structural features characteristic of other laccases, including the conserved regions of four histidine-rich copper-binding sites. The predicted amino acid sequence showed a high homology （more than 60%） with bacterial laccases in the genome and protein databases and the highest degree of similarity （61% identity） was observed with the multicopper oxidase of KlebsieUa sp. 601. When expressed in Escherichia coli, the recombinant enzyme was overproduced in the cytoplasm as soluble and active form. The purified enzyme had an optimum pH of 2.6 and 8.0 for ABTS （2,2＇-azino-bis（3-ethylbenzthiazolinesulfonic acid） and DMP （2,6-dimethoxyphenol）, respectively. The kinetic study on ABTS revealed a higher affinity of this enzyme to this substrate than DMP.

关键词： cloning expression enzymatic properties laccase Aeromonas hydrophila

Isolation and characterization of Pseudomonas otitidis WL-13 and its capacity to decolorize triphenylmethane dyes

在线全文

学校读者我要写书评

暂无评论

Journal of Environmental Sciences 2009年第7期21卷 960-964页

作者： WU Jing JUNG Byung-Gil KIM Kyoung-Sook LEE Young-Choon SUNG Nak-Chang Department of biotechnology Dong-A University Busan 604-714 South Korea Brain Korea 21 center for silver-bio industrialization Dong-A University Busan 604-714 South Korea Department of Environmental Engineering College of Engineering Dong-A University. Busan 604-714 South Korea

Pseudomonas otitidis WL-13, which has a high capacity to decolorize triphenylmethane dyes, was isolated from activated sludge obtained from a wastewater treatment plant of a dyeing industry. This strain exhibited a remarkable color-removal capability when tested against several triphenylmethane dyes under both shaking and static conditions at high concentrations of dyes. More than 95% of Malachite Green and Brilliant Green was removed within 12 h at 500 μmol/L dye concentration under shaking conditions. Crystal Violet lost about 13% of its color under the same conditions tested. The rate of decolorization increased when the M9 medium was supplemented with yeast extract. The optimum pH and temperature for color removal were 7-9 and 35-40℃, respectively. The observed changes in the visible spectra and the inspection of bacterial growth indicated the color-removal by the adsorption of dye to the cells during incubation with strains.

关键词： adsorption decolorization Pseudomonas otitidis WL-13 triphenylmethane dye

The chromosome-level reference genome assembly for Dendrobium officinale and its utility of functional genomics research and molecular breeding study

在线全文

学校读者我要写书评

暂无评论

Acta Pharmaceutica Sinica B 2021年第7期11卷 2080-2092页

作者： Zhitao Niu Fei Zhu Yajuan Fan Chao Li Benhou Zhang Shuying Zhu Zhenyu Hou Mengting Wang Jiapeng Yang Qingyun Xue Wei Liu Xiaoyu Ding College of Life Sciences Nanjing Normal UniversityChina&Jiangsu Provincial Engineering Research Center for Technical Industrialization for DendrobiumsNanjing 210023China

Dendrobium officinale,an important medicinal plant of the genus Dendrobium in Orchidaceae family,has been used as traditional Chinese medicine(TCM)for nearly thousands of ***,we report the first chromosome-level reference genome of ***,based on Pac bio long-reads,Illumina short-reads and HiC *** high-quality assembled genome is 1.23 Gb long,with contig N50 of 1.44 Mb.A total of 93.53%genome sequences were assembled into 19 pseudochromosomes with a super scaffold N50 of 63.07 *** comparative genomic analysis,we explored the expanded gene families of ***,and also their impact on environmental adaptation and biosynthesis of secondary *** further performed detailed transcriptional analysis of ***,and identified the candidate genes involved in the biosynthesis of three main active ingredients,including polysaccharides,alkaloids and *** addition,the MODIFYING WALL LIGNIN-1(MWL1)gene,which inferred from Genome-Wide Association Studies(GWAS)based on the resequencing date from *** and five related species and their morphologic features,may contribute to the plant production(yield of stems)of ***,the high-quality reference genome reported in this study could benefits functional genomics research and molecular breeding of ***.

关键词： Dendrobium officinale Genome Active ingredients GWAS Plant production

Production of Itaconic Acid Through Microbiological Fermentation of Inexpensive Materials

在线全文

学校读者我要写书评

暂无评论

Journal of bioresources and bioproducts 2019年第3期4卷 1-8页

作者： Jing YANG Hao XU Jianchun JIANG Ning ZHANG Jingcong XIE Min WEI Jian ZHAO Institute of Chemical Industry of Forest Products CAFNational Engineering Laboratory for Biomass Chemical UtilizationKey and Open Laboratory of Forest Chemical EngineeringSFAKey Laboratory of Biomass Energy and MaterialJiangsu ProvinceNanjing 210042China Co-Innovation center of Efficient Processing and Utilization of Forest Resources Nanjing Forestry UniversityNanjing 210037China

Itaconic acid(IA)is a precursor of various chemicals,which has been on the US Department of Energy’s list of the top 12 platform chemicals that can potentially be produced from *** IA is mainly produced through a bio-fermentation method with sugar as raw ***,the high cost and low yield restrict the industrial application of the *** review summarized research advances in the IA production from alternative *** particular,the types of substrates as well as existing problems in the IA fermentation,strain breeding,and process regulation were *** it is pointed that decreasing the price of the IA through use of alternative substrates and increasing the titer of the IA through genetic engineering should be further focused on to support the industrialization of the IA.

关键词： itaconic acid fermentation Aspergillus terreus biomass

Comparison of cellular responses across multiple passage numbers in Ba/F3-BCR-ABL cells induced by silver nanoparticles

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2012年第10期55卷 898-905页

作者： GUO DaWei ZHANG XiuYan HUANG ZhiHai ZHOU XueFeng ZHU LingYing ZHAO Yun GU Ning liangsu Key Laboratory for biomaterials and Devices State Key Laboratory of BioelectronicsSchool of Biological Science&Medical EngineeringSoutheast UniversityNanjing 210096China Suzhou Key Laboratory of biomedical Materials and Technology Research Institute of Southeast University in SuzhouSuzhou 215123China Cyrus Tang Hematology center Jiangsu Institute o[HematologyFirst Affiliated HospitalSooehow UniversitySuzhou 215123China

With the rapid development of nanotechnology and increasingly broad bio-application of engineered nanomaterials, their bio- hazards have become a serious public concern. It is believed that the chemical nature, particle size, morphology, and surface chemistry of nanomaterials are key parameters that influence their toxicity. Although cultured ceils have been widely used to evaluate nanomaterial toxicity, it remains unclear whether the passage of these cells affects the evaluation results. In the pre- sent study, Ba/F3 cells transfected with the BCR-ABL gene were subcultured to study the effect of passage number on cell sta- bility and their cellular responses upon exposure to nanomaterials. The results demonstrated that proliferation, cellular senes- cence, BCR-ABL gene expression, cell cycle and apoptosis were stable across multiple passages. Senescence and BCR-ABL gene expression of cells from different passage cells were unchanged when treated with silver nanoparticles （AgNPs）. In addi- tion, the cells at multiple passage numbers were all arrested in the G2/M phase and apoptosis was induced by the AgNPs. These nanoparticles could enter cells via endocytosis and localize in the cndosomes, which were also not influenced by passage number. These data suggest that short-term passage would not affect cultured cell stability and toxicity assessment using these cells would be consistent when maintained appropriately.

关键词： silver nanoparticles (AgNPs) cellular response cell passage stability uptake