Isolation and characterization of Pseudomonas otitidis WL-13 and its capacity to decolorize triphenylmethane dyes

作     者：WU Jing JUNG Byung-Gil KIM Kyoung-Sook LEE Young-Choon SUNG Nak-Chang 

作者机构：Department of Biotechnology Dong-A University Busan 604-714 South Korea Brain Korea 21 Center for Silver-Bio Industrialization Dong-A University Busan 604-714 South Korea Department of Environmental Engineering College of Engineering Dong-A University. Busan 604-714 South Korea 

出 版 物：《Journal of Environmental Sciences》 (环境科学学报（英文版）)

年 卷 期：2009年第21卷第7期

页      面：960-964页

核心收录：

学科分类：0832[工学-食品科学与工程（可授工学、农学学位）] 083002[工学-环境工程] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 08[工学] 083201[工学-食品科学] 

基　　金：supported by the Dong-A University Research Fund in 2007 

主　　题：adsorption decolorization Pseudomonas otitidis WL-13 triphenylmethane dye 

摘      要：Pseudomonas otitidis WL-13, which has a high capacity to decolorize triphenylmethane dyes, was isolated from activated sludge obtained from a wastewater treatment plant of a dyeing industry. This strain exhibited a remarkable color-removal capability when tested against several triphenylmethane dyes under both shaking and static conditions at high concentrations of dyes. More than 95% of Malachite Green and Brilliant Green was removed within 12 h at 500 μmol/L dye concentration under shaking conditions. Crystal Violet lost about 13% of its color under the same conditions tested. The rate of decolorization increased when the M9 medium was supplemented with yeast extract. The optimum pH and temperature for color removal were 7-9 and 35-40℃, respectively. The observed changes in the visible spectra and the inspection of bacterial growth indicated the color-removal by the adsorption of dye to the cells during incubation with strains.