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food Bioscience 2022年第3期47卷 1358-1367页

作者： Ricardo Gómez-García Ana A.Vilas-Boas Manuela Machado Débora A.Campos Cristóbal N.Aguilar Ana R.Madureira Manuela Pintado Universidade Católica Portuguesa CBQF-Centro de Biotecnologia e Química Fina – Laboratório AssociadoEscola Superior de BiotecnologiaRua Diogo Botelho 13274169-005PortoPortugal bbg-dia.bioprocesses and bioproducts group.food research department School of ChemistryAutonomous University of CoahuilaSaltilloCoahuilaMexico

The objectives of this research work were to evaluate the effect of in vitro gastrointestinal digestion (GIT) on melon peel juice (MPJ) powder from fruit processing industry by-products,considering (i) the recovery and accessibility indexes,(ii) the changes on antioxidant activity,and (iii) the prebiotic *** exposition to GIT conditions a decrease on the total phenolic content (TPC = 65.31%) and antioxidant activity by ABTS = 39.77% and DPPH = 45.91% were ***,these both parameters exhibited stable accessibility,accounting with 81.89%,76.55%,and 54.07% for TPC,ABTS and DPPH,*** gastrointestinal digestion,the non-absorbed fraction exhibited a positive impact on the growth of Bifidobacterium and Lactobacillus strains,possibly associated with the high content of simple sugar (glucose and fructose).This fraction also showed to be safe on Caco-2 intestinal *** findings suggest that MPJ might be used as a potential food functional ingredient.

关键词： Melon by-products Functional ingredients Phenolic antioxidants Simulated digestion Prebiotic effect

Ultrasound-assisted extraction of antioxidant polyphenolic compounds from Nephelium lappaceum L.(Mexican variety) husk

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Asian Pacific Journal of Tropical Medicine 2018年第12期11卷 676-681页

作者： Adriana Méndez-Flores Ayerim Hérnandez-Almanza Aidé Sáenz-Galindo Jesús Morlett-Chávez Cristóbal N Aguilar Juan Ascacio-Valdés bioprocesses and bioproducts research group Food Research Department School of Chemistry Autonomous University of Coahuila Organic Chemistry department School of Chemistry Autonomous University of Coahuila Molecular Biology Laboratory School of Chemistry Autonomous University of Coahuila

Objective: To reach the recovery and identification of antioxidant polyphenolic compounds from Nephelium lappaceum L.(Mexican variety) husk using ultrasound-assisted extraction and liquid chromatography/mass spectrometry as well as the in vitro antioxidant activity. Methods: Rambutan husk extracts were obtained by ultrasound-assisted extraction, mass/volume ratio, water/ethanol percentage and extraction time were evaluated. Once the best extraction condition of polyphenolic compounds was defined, a polyphenolic fraction was recovered using Ambetlite XAD-16. The total content of antioxidant polyphenolic compounds was determined by summation of the total hydrolysable polyphenol and total condensed polyphenol contents. Recovered compounds were identified by FTIR(ATR) spectroscopy and HPLC/ESI/MS. The antioxidant activity was carried out by ABTS, DPPH and lipid oxidation inhibition in vitro methods. Results: In Mexican variety rambutan husk, the total polyphenolic content was 487.67 mg/g, after ultrasound-assisted extraction. According to the HPLC/ESI/MS analysis 12 antioxidant polyphenolic compounds were identified, mostly ellagitannins such as geraniin, corilagin and ellagic acid. The antioxidant activity determined by ABTS, DPPH and lipid oxidation inhibition methods was demonstrated. The main functional groups of the identified compounds were determined by FTIR analysis. Conclusions: It was demonstrated that ultrasound-assisted extraction was effective and allowed the extraction and recovery of antioxidant polyphenolic compounds. Furthermore Mexican variety rambutan husk is an important source for recovering polyphenolic compounds with antioxidant activity, these compounds have potential application for the treatment/prevention of various diseases related to cancer and pathogenic microorganisms.

关键词： Mexican variety rambutan Ultra-sound assisted extraction Antioxidant polyphenolic compounds HPLC/ESI/MS

Bioactive Compounds Recovery from Larrea tridentata by Green Ultrasound- Assisted Extraction

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Phyton-International Journal of Experimental Botany 2024年第11期93卷 3091-3107页

作者： Muyideen Olaitan Bamidele Jose Sandoval-Cortes Maria Liliana Flores-Lopez Olga Berenice Alvarez Perez Monica Lizeth Chavez Gonzalez Cristobal Noe Aguilar bioprocesses and bioproducts research group Food Research DepartmentSchool of ChemistryUniversidad Autonoma de CoahuilaSaltillo25070Mexico Centro de Investigacion e Innovacion Cientifica y Tecnologica Universidad Autonoma de CoahuilaSaltillo25070Mexico

Postharvest losses remain a signiﬁcant challenge,particularly in developing countries that have inadequate *** plants offer an eco-friendly and sustainable solution for managing diseases and pests in agri-cultural *** plants are rich in bioactive compounds,such as alkaloids,ﬂavonoids,terpenoids,phenolics,and essential oils,many of which have proven antimicrobial,antifungal,insecticidal,and antioxidant *** characteristics make medicinal plants viable candidates for postharvest disease and pest *** these,Larrea tridentata(creosote bush)is particularly notable for its bioactive compounds with strong antifungal *** potential applications include agriculture,food preservation,and *** study aimed to evaluate how different solvent mixtures affect the efﬁciency of ultrasound-assisted extraction,total polyphenol content,antioxidant capacity,and antifungal activity of *** leaves and ***ﬁndings revealed that the 60%ethanol ultrasound-assisted extract of *** leaves(ULL 60%)contained the highest concentration of bioactive compounds,including hydrolysable tannins(690.2 mg GAE/100 g)and condensed tan-nins(329.9 mg CE/100 g).All extracts demonstrated notable antioxidant activity in ABTS,DPPH,and FRAP assays,with ethanol-based extracts showing greater antioxidant potential than their aqueous *** terms of antifungal efﬁcacy,100%ethanol leaf extract exhibited the strongest inhibition against Fusarium oxyspor-um(60.03%),whereas 50%ethanol extract effectively inhibited Alternaria alternata(53.61%).Six major polyphe-nolic compounds were identiﬁed using reverse-phase high-performance liquid chromatography coupled with electrospray ionization mass spectrometry(RP-HPLC-ESI-MS).These include quercetin,luteolin,3,4-dihydrox-yphenylethanol,elenolic acid,nordihydroguaiaretic acid(NDGA),and *** compounds are known to have antibacterial,antifungal and antioxidant propert

关键词： Phytopathogens bioactive compounds desert plants Fusarium oxysporum Alternaria alternata

Development and characterization of whey protein films incorporated with tarbush polyphenols and candelilla wax

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food Bioscience 2022年第1期45卷 1-7页

作者： Olga B.Alvarez-Perez Janeth M.Ventura-Sobrevilla Cristian Torres-Leon Romeo Rojas-Molina Raúl Rodríguez-Herrera Miguel A.Aguilar-Gonzalez Cristobal N.Aguilar bioprocesses and bioproducts research group Food Research DepartmentSchool of ChemistryUniversidad Autonoma de Coahuila25280SaltilloCoahuilaMexico School of Health Sciences Universidad Autonoma de Coahuila26090Piedras NegrasCoahuilaMexico School of Agronomy Research Center and Development for Food IndustryUniversidad Autonoma de Nuevo Leon6050General EscobedoNuevo LeonMexico Center for research and Advanced Studies of the National Polytechnique Institute Parque Industrial Saltillo-Ramos ArizpeCoahuilaMexico

Edible films were prepared with whey protein incorporating candelilla wax,and glycerol in aqueous *** polyphenols were added as bioactive *** optimization based on the water vapor permeability(WVP)of the formulation was performed using a Box-Behnken *** films were characterized by the determination of WVP,moisture,solubility,color,antioxidant and antifungal *** electron microscopy(SEM)was also performed to observe the surface and cross-section *** lowest permeability values were found with concentrations of 1.27,0.3 and 0.5% of protein,wax and glycerol *** was proved that the addition of polyphenols into films causes a significant effect only on solubility and transparency *** films have suitable properties as a barrier for the goods they could be applied *** is proposed for use in foods such as fresh or minimally processed fruits and vegetables.

关键词： Edible films Whey protein Shelf life Candelilla wax Euphorbia antisyphilitica Fluorensia cernua polyphenols

Bacteriocins as antimicrobial and preservative agents in food:Biosynthesis,separation and application

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food Bioscience 2022年第2期46卷 749-771页

作者： Deepak Kumar Verma Mamta Thakur Smita Singh Soubhagya Tripathy Alok Kumar Gupta Deepika Baranwal Ami R.Patel Nihir Shah Gemilang Lara Utama Alaa Kareem Niamah M´onica L.Ch´avez-Gonz´alez Carolina Flores Gallegos Cristobal Noe Aguilar Prem Prakash Srivastav Agricultural and food Engineering department Indian Institute of Technology KharagpurKharagpur721302West BengalIndia department of food Technology School of SciencesITM UniversityGwalior474001Madhya PradeshIndia department of Nutrition and Dietetics University Institute of Applied Health SciencesChandigarh UniversityChandigarh140413PunjabIndia Division of Post-harvest Management ICAR-Central Institute for Subtropical Horticulture(Ministry of Agriculture and Farmers WelfareGovernment.of India)Lucknow(UP)226101India department of Home Science Arya Mahila PG CollegeBanaras Hindu UniversityVaranasiUttar Pradesh221005India Division of Dairy Microbiology Mansinhbhai Institute of Dairy and Food Technology-MIDFTDudhsagar Dairy CampusMehsana384002GujaratIndia Faculty of Agro-Industrial Technology Universitas PadjadjaranSumedang45363Indonesia Center for Environment and Sustainability Science Universitas PadjadjaranBandung40132Indonesia department of food Science College of AgricultureUniversity of BasrahBasra CityIraq bioprocesses and bioproducts research group and Nanobioscience group.food research department School of Chemistry.Autonomous University of CoahuilaSaltillo Campus25280CoahuilaMexico

Emerging evidence shows the trend of using safe and natural preservatives like bacteriocins in food *** increasing demand by the food industry to extend keeping quality and prevent spoilage of various food products has appealed for new preservatives and new methods of ***,to discover the novel spectrum of antimicrobial compounds that could effectively battle food-borne pathogens,bacteriocins have been intensively studied in the last few ***,great attention has been paid to the application of lactic acid-bacterial bacteriocins targeting food spoilage-causing or pathogenic microorganisms with no significant side *** mechanisms of action like pore-formation,retarding cell-wall/nucleic acid/protein synthesis have been proposed and described for diverse ***,the syntheses,purification of bacteriocins in addition to their applications as antimicrobial and preservative agents in food processing are reviewed in *** review will also discuss the preservative effect of bacteriocins and their combinations having multiple modes of action(hurdle approach)to reduce the microbial load as well as antimicrobial resistance.

关键词： Lactic acid bacteria Bacteriocins Preservative food-borne pathogens food processing

Use of wastes from the tea and coffee industries for the production of cellulases using fungi isolated from the Western Ghats of India

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Systems Microbiology and Biomanufacturing 2021年第1期1卷 33-41页

作者： S.A.Saldana-Mendoza J.A.Ascacio-Valdes A.S.Palacios-Ponce J.C.Contreras-Esquivel R.Rodriguez-Herrera H.A.Ruiz J.L.Martinez-Hernandez S.Sugathan C.N.Aguilar bioprocesses and bioproducts research group Food Research DepartmentAutonomous University of Coahuila25280 SaltilloMexico Faculty of Engineering in Mechanics and Production Sciences Polytechnic School of LitoralESPOLCampus Gustavo Galindo Km.30.5 Vía PerimetralP.O.Box 09-01-5863GuayaquilEcuador Microbiology Division Jawaharial Nehru Botanical Garden and Research InstitutePalodeThiruvananthapuramKerala 695562India

In this study,coffee pulp(Coffea arabica)and green tea(Camellia sinensis)residues were characterized for use as a substrate of solid-state fermentation for cellulases *** invasion rate was evaluated,as well as cellulases production by strains of Aspergillus niger and Trichoderma asperellum from the western Ghats of India,on coffee pulp,green tea,and a mixture of both substrates(50:50).***(AFP)strain was found to have the highest growth rate(0.409±0.021 mm/h)using a mixture of both *** production of cellulases by *** was unsatisfactory due to the presence of polyphenols in the supports to which *** cellulases are more *** production of cellulases by *** was linked to the pH of the supports,favouring the use of T and *** was found that the extracts produced by ***(28A strain using a mixture substrate,28A,and 20A strains using only green tea as a substrate)presented the highest cellulase activities when evaluated using a plate technique producing degradation halos of 2.3±0.1 cm of *** 28A strain did not require mineral enrichment media for cellulase production using green tea residues as support of solid-state fermentation.

关键词： Cellulases Fungal strains Solid-state fermentation Agroindustrial wastes Coffee pulp Green tea

Immobilization of Chitosanase on Magnetic Nanoparticles: Preparation, Characterization and Properties

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Journal of Ocean University of China 2022年第5期21卷 1381-1388页

作者： CHENG Yimeng LI Zhaoyue SUN Huihui ZHAO Ling LIU Zhen CAO Rong LIU Qi MAO Xiangzhao College of food Science and Engineering Ocean University of ChinaQingdao 266003China department of food Engineering and Nutrition Yellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdao 266071China Laboratory for Marine Drugs and bioproducts of Qingdao National Laboratory for Marine Science and TechnologyQingdao 266237China

Chitosanase could cleaveβ-1,4-linkage of chitosan to produce chitooligosaccharides(COS)with diverse biological ***,there are many limitations on the use of free chitosanase in industrial *** immobilization is generally considered a valuable strategy in industrial-scale *** this study,the chitosanase Csn-BAC from Bacillus ***-5 was immobilized on Fe_(3)O_(4)-SiO_(2) magnetic nanoparticles(MNPs)to enhance its properties,which could be recovered easily from reaction media using magnetic *** activities of Csn-BAC immobilized with MNPs(MNPs@Csn-BAC)were de-termined with temperature and pH,and the thermal-and pH-stabilities,*** reusability of the MNPs@Csn-BAC was determined in repeated reaction *** enhanced the thermal and pH stability of Csn-BAC compared with the free *** eight reaction cycles using MNPs@Csn-BAC,the residual enzyme activity was 72.15%.Finally,the amount of COS released by MNPs@Csn-BAC was 1.86 times higher than that of the free Csn-BAC in the catalytic performance *** immobilized Csn-BAC exhibits broad application prospects in the production of COS.

关键词： chitosanase immobilization magnetic nanoparticles chitooligosaccharides

Comparative Study on Antineoplastic Activity of Polysaccharide from Four Kinds of Sea Cucumber

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Journal of Ocean University of China 2018年第6期17卷 1473-1478页

作者： ZHAO Ling LIU Qi YANG Xianqing CAO Rong department of food Engineering and Nutrition Yellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdao 266071China Key Laboratory of Aquatic Product Processing Ministry of AgricultureGuangzhou 510300China Laboratory for Marine Drugs and bioproducts of Qingdao National Laboratory for Marine Science and Technology Qingdao 266071China

The antineoplastic activity of polysaccharide was investigated in Stichopus chlorontus, Isostichopus badionotus, Stichopus horrens and Holothuria lessoni massin. Crude polysaccharide was prepared with enzyme hydrolyzation method and purified by anion exchange chromatography using DEAE-sepharose fast flow column. The effect of polysaccharide on cells apoptosis of SiHa and U87 was examined with cell counting kit-8 colorimetry method. Western blotting was used to analyze related proteins of cellular apoptosis including p53 and Bcl-2. Results showed that there were two main components in each sea cucumber polysaccharide, which could be eluted down by 1.0 mol/LNaCl solution. The four types of polysaccharide in the second component were named as SC-2, IB-2, HLM-2 and SH-2, respectively. They were used for comparing the antineoplastic activity. Results showed that SC-2, IB-2, HLM-2 and SH-2 could promote apoptosis of U87 and SiHa cells. SH-2 and HLM-2 were selected for the subsequent experiment to explore the additional effect of U87 and SiHa cells, The protein expressions of Bcl-2 and p53 decreased considerably with the increase of polysaccharide concentration in U87 cells. In SiHa cells, protein expressions of Bcl-2 and high dosage group of p53 decreased significantly, whereas no obvious decrease was observed in other groups. The polysaccharides are more effective in promoting apoptosis of U87 and SiHa cells from S. horrens and H. lessoni massin than from the rest species.

关键词： sea cucumber polysaccharide antineoplastic activity

Conversion of Biomass to Hydrocarbon-rich Bio-oil via Microwave-assisted Catalytic Pyrolysis： A Review

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China Petroleum Processing & Petrochemical Technology 2018年第3期20卷 7-16页

作者： Wang Yunpu Zhang Shumeil Yu Zhenting Jiang Lin Liu Yuhuan Ruan Roger Fu Guiming Nanchang University State Key Laboratory of Food Science and Technology Nanchang 330047 Nanchang University Engineering Research Center for Biomass Conversion Ministry of Education Nanchang 330047 Guangdong Provincial Key Laboratory of New and Renewable Energy research and Development Guangzhou 510640 Center for Biorefining and department of bioproducts and Biosystems Engineering University of Minnesota USA

The method for pyrolysis of biomass to manufacture hydrocarbon-rich fuel remains challenging in terms of conversion of multifunctional biomass with high oxygen content and low thermal stability into a high-quality compound, featuring high content of hydrocarbons, low oxygen content, few functional groups, and high thermal stability. This study offers a promising prospect to derive hydrocarbon-rich oil through microwave-assisted fast catalytic pyrolysis by improving the effective hydrogen to carbon ratio(H/Ceff) of the raw materials. The proposed technique can promote the production of high-quality bio-oil through the molecular sieve catalyzed reduction of oxygenated compounds and mutagenic polyaromatic hydrocarbons. This work aims to review and summarize the research progress on microwave copyrolysis and microwave catalytic copyrolysis to demonstrate their benefits on enhancement of bio-oils derived from the biomass. This review focuses on the potential of optimizing the H/Ceff ratio, the microwave absorbent, and the HZSM-5 catalyst during the microwave copyrolysis to produce the valuable liquid fuel. This paper also proposes future directions for the use of this technique to obtain high yields of bio-oils.

关键词： biomass microwave pyrolysis H/Ceff microwave absorbent HZSM-5 catalyst hydrocarbon-rich bio-oil

Determination of Physical, Mechanical and Fire Retardancy Properties of Innovative Particleboard Made from Corn Stalk (Zea mays L.) Particles

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Journal of Renewable Materials 2024年第10期12卷 1729-1756页

作者： Lilik Astari Benoit Belleville Kenji Umemura Alex Filkov Barbara Ozarska Robert H.Crawford School of Agriculture Food and Ecosystem SciencesThe University of MelbourneMelbourne3121Australia research Center for Biomass and bioproducts National Research and Innovation AgencyBogor16911Indonesia research Institute of Sustainable Humanosphere Kyoto UniversityKyoto611-0011Japan School of Agriculture Food and Ecosystem SciencesThe University of MelbourneCreswick3363Australia Faculty of Architecture Building and PlanningThe University of MelbourneMelbourne3010Australia

The demand for particleboard is increasing along with economic and population ***,two major barriers to the manufacture of particleboard are a shortage of raw materials(woodchips)and the emission of formaldehyde from conventional *** by-products such as corn stalks contain an abundance of renewable lignocellulosic *** study evaluates the effect of citric acid as a natural adhesive and fire retardant addition on the physical,mechanical,and fire retardancy properties of particleboards fabricated from corn stalks.A cost-effective and inorganic salt,calcium carbonate,was tested to enhance the fire *** dihydrogen phosphate was also considered as a comparative *** with the addition of calcium carbonate was pretreated with sodium *** particleboards were pressed for 10 min at 200℃.Japanese Industrial Standard JIS A 5908:2022 was used as the benchmark for the physical and mechanical *** retardancy was dynamically tested by simulating a Bushfire Attack Level of 19 kW/m^(2).The particleboard with 25 wt%citric acid had superior mechanical properties and complied with the JIS A 5908 standard for Type 13 base *** with the addition of calcium carbonate(5%and 10%)showed significantly delayed pyrolysis time.

关键词： Particleboard corn stalk fire retardancy citric acid mechanical properties