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rna interference of a trehalose-6-phosphate synthase gene reveals its roles in the biosynthesis of chitin and lipids in Heortia vitessoides(Lepidoptera:Crambidae)

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Insect Science 2020年第2期27卷 212-223页

作者： Jing-Xiang Chen Zi-Hao Lyu Chun-Yan Wang Jie Cheng Tong Lin College of Forestry and Landscape architecture South China Agricultural UniversityGuangzhouChina

Trehalose 6-phosphate synthase(TPS),an enzyme that hydrolyzes two glucose molecules to yield trchalose,plays a pivotal role in various physiological *** this study,we cloned the trehalose-6-phosphate synthase gene(HvTPS)and investigated its expression patterns in various tssues and d:velopmental stages in Heortia vitessoides Moore(Lepidoptera:Crambidac).HvTPS was highly expressed in the fat body and after pupation or before *** knocked down TPS in *** by rna interference and found that 3.0μg of dsHvTPS resulted in optimal interference at 24 h and 36 h post-injection and caused a sharp decline in the survival rate during the 5th instar larval-pupal stage and obviously abnormal or lethal *** to the controls,TPS activity and trehalose contents were significantly lower and the glucose content was significantly higher 24 h or 36 h after injection with 3.0μg of ***,the silencing of HvTPS suppressed the cxpression of six key genecs in the chitin biosynthesis pathway and one key gene related to lipid *** expression levels of two genes associated with lipid biosynthesis were *** results strongly suggest that HvTPS is essential for the normal growth and development of *** and provide a reference for further studies of the utility of key genes involved in chitin and lipid biosynthesis for controlling insect development.

关键词： chitin biosynthesis Heortia vitessoides Moore lipid biosynthesis metamor-phosis rna interference trehalose-6-phosphate synthase

A KH Domain-Containing Putative rna-Binding Protein Is Critical for Heat Stress-Responsive Gene Regulation and Thermotolerance in Arabidopsis

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Molecular Plant 2013年第2期6卷 386-395页

作者： Qingmei Guan Changlong Wen Haitao Zeng Jianhua Zhu Department of Plant Science and Landscape architecture University of Maryland College Park MD 20742 USA

Heat stress is a severe environmental factor that significantly reduces plant growth and delays develop-ment. Heat stress factors （HSFs） are a class of transcription factors that are synthesized rapidly in response to elevations in temperature and are responsible for the transcription of many heat stress-responsive genes including those encoding heat shock proteins （HSPs）. There are 21 HSFs in Arabidopsis, and recent studies have established that the HSFA1 family members are master regulators for the remaining HSFs. However, very little is known about upstream molecular factors that control the expression of HSFA1 genes and other HSF genes under heat stress. Through a forward genetic analysis, we identified RCF3, a K homology （KH） domain-containing nuclear-localized putative rna-binding protein. RCF3 is a neg- ative regulator of most HSFs, including HSFAla, HSFAlb, and HSFAld. In contrast, RCF3 positively controls the expression of HSFAle, HSFA3, HSFAg, HSFB3, and DREB2C. Consistently with the overall increased accumulation of heat-responsive genes, the rcf3 mutant plants are more tolerant than the wild-type to heat stress. Together, our results suggest that a KH domain-containing putative rna-binding protein RCF3 is an important upstream regulator for heat stress-responsive gene expression and thermotolerance in Arabidopsis.

关键词： rna-binding protein RCF3 HSF HSR

Aberrant expression of enzymes regulating m^6A mrna methylation: implication in cancer

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Cancer Biology & Medicine 2018年第4期15卷 323-334页

作者： Natalia Pinello Stephanie Sun Justin Jong-Leong Wong Epigenetics and rna Biology Program Centenary Institute The University of Sydney Sydney Medical School The University of Sydney

N^6-methyladenosine(m^6 A) is an essential rna modification that regulates key cellular processes, including stem cell renewal,cellular differentiation, and response to DNA damage. Unsurprisingly, aberrant m^6 A methylation has been implicated in the development and maintenance of diverse human cancers. Altered m^6 A levels affect rna processing, mrna degradation, and translation of mrnas into proteins, thereby disrupting gene expression regulation and promoting tumorigenesis. Recent studies have reported that the abnormal expression of m^6 A regulatory enzymes affects m^6 A abundance and consequently dysregulates the expression of tumor suppressor genes and oncogenes, including MYC, SOCS2, ADAM19, and PTEN. In this review, we discuss the specific roles of m^6 A missing space "writers", "erasers", and "readers" in normal physiology and how their altered expression promotes tumorigenesis. We also describe the potential of exploiting the aberrant expression of these enzymes for cancer diagnosis, prognosis, and the development of novel therapies.

关键词： rna modification N^6-methyladenosine (m^6A) cancer tumor suppressor oncogene

Viroids:Small Probes for Exploring the Vast Universe of rna Trafficking in Plants

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Journal of Integrative Plant Biology 2010年第1期52卷 28-39页

作者： Ying Wang Biao Ding Department of Plant Cellular and Molecular Biology and Plant Biotechnology Center The Ohio State University The Center for rna Biology The Ohio State University Molecular Cellular and DeVelopmental Biology Program The Ohio State University Columbus OH 43210 USA

Cell-to-cell and long-distance trafficking of rna is a rapidly evolving frontier of integrative plant biology that broadly impacts studies on plant growth and development, spread of infectious agents and plant defense responses. The fundamental questions being pursued at the forefronts revolve around function, mechanism and evolution. In the present review, we will first use specific examples to illustrate the biological importance of cell-to-cell and long-distance trafficking of rna. We then focus our discussion on research findings obtained using viroids that have advanced our understanding of the underlying mechanisms involved in rna trafficking. We further use viroid examples to illustrate the great diversity of trafficking machinery evolved by plants, as well as the promise for new insights in the years ahead. Finally, we discuss the prospect of integrating findings from different experimental systems to achieve a systems-based understanding of rna trafficking function, mechanism and evolution.

关键词： VIROIDS PLANT defenses rna PLANT evolution PLANT cells & tissues

Interaction effects of DNA, rna-polymerase, and cellular fluid on the local dynamic behaviors of DNA

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Applied Mathematics and Mechanics(English Edition) 2020年第4期41卷 623-636页

作者： Weipeng HU Zichen DENG School of Civil Engineering and architecture Xi’an University of TechnologyXi’an 710048China State Key Laboratory of Eco-Hydraulics in Northwest Arid Region of China Xi’an University of TechnologyXi’an 710048China School of Mechanics Civil Engineering and ArchitectureNorthwestern Polytechnical UniversityXi’an 710072China

In view of the complex structure and environment,the dynamic analysis on deoxyribonucleic acid(DNA)is a challenge in the biophysics *** the local interaction with ribonucleic acid(rna)-polymerase as well as the dissipative effect of cellular fluid,a coupling sine-Gordon-type dynamic model is used to describe the rotational motions of the bases in ***,the approximate symmetric form is ***,the wave form and the wave velocity of the kink solution to the proposed dynamic model are investigated by a Runge-Kutta structure-preserving scheme based on the generalized multi-symplectic *** numerical results indicate that,the strengthening of the local interaction between DNA and rna-polymerase described by the coupling potential makes the form of the kink solution steep,while the appearance of the friction between DNA and cellular fluid makes the form of the kink solution *** addition,the appearance of the friction decreases the velocities of both the symplectic configuration and the anti-symplectic configuration with different *** above findings are beneficial to comprehend the DNA transcription mechanism.

关键词： deoxyribonucleic acid (DNA) dynamics local interaction generalized multisymplectic idea kink solution Runge-Kutta scheme

长链非编码rna与弥漫性大B细胞淋巴瘤关系的研究进展

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中国实验血液学杂志 2018年第2期26卷 609-614页

作者：吴艳珺邢依然郁多男扬州大学非编码rna研究中心江苏省非编码RNA基础与临床转化重点实验室江苏扬州225001

弥漫性大B细胞淋巴瘤(diffuse large B-cell lymphoma,DLBCL)发病机制未明,治疗效果差,发生率与死亡率仍在不断攀升,因此探究DLBCL的发病机制与发现新的分子诊断标记和治疗靶点尤为重要。长链非编码rna(long non-coding rna,lnc rna)是... 详细信息

弥漫性大B细胞淋巴瘤(diffuse large B-cell lymphoma,DLBCL)发病机制未明,治疗效果差,发生率与死亡率仍在不断攀升,因此探究DLBCL的发病机制与发现新的分子诊断标记和治疗靶点尤为重要。长链非编码rna(long non-coding rna,lnc rna)是真核细胞中一类转录本长度大于200 bp的rna,可在转录水平及转录后水平调控其靶基因的表达,并参与多种肿瘤的发生、发展、侵袭及转移等过程。近年来长链非编码rna在弥漫性大B细胞淋巴瘤中的作用不断被发现及证实。本文就HULC、PEG10、Linc rna-p21、HOTAIR、LUNAR1、MALAT1和SubSig Lnc-17等长链非编码rna与弥漫性大B细胞淋巴瘤的关系做一综述,希望为相关基础研究及临床诊断和治疗提供新思路。

关键词：长链非编码rna 弥漫性大B细胞淋巴瘤肿瘤标记物治疗靶点

ISOLATION AND RECONSTITUTION OF THE dsrna-DEPENDENT rna POLYMERASE OF CYTOPLASMIC POLYHEDROSIS VIRUS OF SILKWORM (Bombyx mori)

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Chinese Science Bulletin 1987年第3期32卷 187-191页

作者：巫爱珍孙玉昆 Shanghai Institute of Biochemistry Academia Sinica Shanghai Institute of Biochemistry Academia Sinica? INTRODUCTION The cytoplasmic polyhedrosis virus (CPV)of the silkworm is one of the double-stranded RNA (dsRNA) virus groupt. The virus particles of CPV contain 10 segments of the double-stranded RNA genome and the RNA replicases. As mentioned in the previous paper the RNA replicase was isolated in the form of genome-replicase and

Ⅰ. INTRODUCTION The cytoplasmic polyhedrosis virus (CPV)of the silkworm is one of the double-stranded rna (dsrna) virus groupt. The virus particles of CPV contain 10 segments of the double-stranded rna genome and the... 详细信息

关键词： rna Bombyx stranded silkworm cytoplasmic Genome contained DEAE mentioned remove

DRSP:蛋白质单残基替换结构数据库（英文）

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生物化学与生物物理进展 2016年第8期43卷 810-816页

作者：刘继龙苗智超李雷肖智雄曹洋四川大学生命科学学院生物资源与生态环境教育部重点实验室生长代谢衰老研究中心成都610064 architecture and reactivity of the rna University of Strasbourg Institute of Molecular and Celluar Biology of CNRS67000 Strasbourg France

蛋白质残基替换是基因突变的产物之一,它可能改变蛋白质三维结构,对其生物学功能产生重大影响,因此研究蛋白质残基替换与结构改变的关系具有重要意义.随着实验解析蛋白质结构的数量迅猛增长,越来越多的野生型-突变体被应用于结构生物学... 详细信息

蛋白质残基替换是基因突变的产物之一,它可能改变蛋白质三维结构,对其生物学功能产生重大影响,因此研究蛋白质残基替换与结构改变的关系具有重要意义.随着实验解析蛋白质结构的数量迅猛增长,越来越多的野生型-突变体被应用于结构生物学的比较研究中.本研究从蛋白质三维结构数据库(PDB)出发,收集和计算了大量结构特征数据,构建了一个目前已知最大的野生型-突变体(单残基差异)的结构对数据库DRSP,展示出氨基酸类型和主链偏好性对结构保守性的相关性.DRSP的开放使用可为高精度的蛋白质结构分析预测提供有用信息,它的数据库网址是http://***/drsp/***.

关键词：数据库蛋白质残基替换主链柔性蛋白质结构预测蛋白质设计

Large-scale study of long non-coding rna functions based on structure and expression features

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Science China(Life Sciences) 2013年第10期56卷 953-959页

作者： ZHAO Yi WANG Jian CHEN XiaoWei LUO HaiTao ZHAO YunJie XIAO Yi CHEN RenSheng Bioinformatics Research Group Advanced Computing Research LaboratoryInstitute of Computing TechnologyChinese Academy ofSciences Department of Physics Huazhong University of Science and Technology Laboratory of Bioinformatics and Non-coding rna Institute of BiophysicsChinese Academy of Sciences

Mammals and other complex organisms can transcribe an abundance of long non-coding rnas(lncrnas)that fulfill a wide variety of regulatory roles in many biological *** roles,including as scaffolds and as guides for protein-coding genes,mainly depend on the structure and expression level of *** this review,we focus on the current methods for analyzing lncrna structure and expression,which is basic but necessary information for in-depth,large-scale analysis of lncrna functions.

关键词： non-coding rna structure expression function