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Potential treatment with Chinese and Western medicine targeting NSP14 of SARS-CoV-2

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Journal of Pharmaceutical Analysis 2021年第3期11卷 272-277页

作者： Chao Liu Xiaoxiao Zhu Yiyao Lu Xianqin Zhang Xu Jia Tai Yang Non-coding rna and Drug Discovery Key Laboratory of Sichuan Province Chengdu Medical CollegeChengduSichuan610500China Basic Medical School Chengdu Medical CollegeChengduSichuan610500China School of Pharmacy Chengdu Medical CollegeChengduSichuan610500China

The outbreak of coronavirus disease 2019(COVID-19)caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)is a serious global health *** raises an urgent need for the development of effective drugs against the deadly ***-CoV-2 non-structural protein 14(NSP14)carrying rna cap guanine N7-methyltransferase and 30-50 exoribonuclease activities could be a potential drug target for ***14 of SARS-CoV-2 shares 98.7%of similarity with the one(PDB 5NFY)of acute respiratory syndrome(SARS)by ***,the SARS-CoV-2 NSP14 structures were modelled by Modeller 9.18 using SARS NSP14(PDB 5NFY)as template for virtual *** on the docking score from AutoDock Vina1.1.2,18 small molecule drugs were selected for further *** on the 5 ns MD simulation trajectory,binding free energy(DG)was calculated by MM/GBSA *** calculated binding free energies of Saquinavir,Hypericin,Baicalein and Bromocriptine for the N-terminus of the homology model wereà37.2711±3.2160,à30.1746±3.1914,à23.8953±4.4800,andà34.1350±4.3683 kcal/mol,respectively,while the calculated binding free energies wereà60.2757±4.7708,à30.9955±2.9975,à46.3099±3.5689,andà59.8104±3.5389 kcal/mol,respectively,when binding to the ***,the compounds including Saquinavir,Hypericin,Baicalein and Bromocriptine could bind to the N-terminus and C-terminus of the homology model of the SARS-CoV-2 NSP14,providing a candidate drug against SARS-CoV-2 for further study.

关键词： SARS-CoV-2 Nonstructural protein 14(NSP14) ZINC database Drug docking

Supramolecular Polymerization of Hydrogen-Bonded Trimers in Bulk and Aqueous Medium

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CCS Chemistry 2024年第6期6卷 1458-1465页

作者： Freek V.de Graaf Stef A.H.Jansen Sandra M.C.Schoenmakers Bas van Genabeek Marle E.J.Vleugels Bart W.L.van den Bersselaar E.W.Meijer Ghislaine Vantomme Institute for Complex Molecular Systems Laboratory of Macromolecular and Organic ChemistryEindhoven University of Technology5600 MBEindhoven School of Chemistry and rna Institute University of New South WalesSydneyNew South Wales 2052

Synthetic molecules that form functional noncovalent assemblies in water have received much attention for their potential applications as *** this work,we introduce a novel water-compatible monomer based on the phthalhydrazide moiety that assembles into hydrogen-bonded trimeric *** bulk,the material exhibits a cylindrical nanostructure in the liquid-crystalline phase at elevated temperatures,morphologically distinct from the crystalline lamellar *** water,these molecules effectively form cylinders in a one-dimensional fashion,yielding fibrous *** formation of these supramolecular polymers follows a cooperative mechanism,as evidenced by denaturation *** trimerized pattern represents a new category of aqueous su pramolecular polymers with future prospects for functional complex molecular systems.

关键词： noncovalent chemistry supramolecular polymers hydrogen bonding cooperativity nanostructure

An optimized high-throughput SARS-CoV-2 dual reporter trans-complementation system for antiviral screening in vitro and in vivo

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Virologica Sinica 2024年第3期39卷 447-458页

作者： Yingjian Li Xue Tan Jikai Deng Xuemei Liu Qianyun Liu Zhen Zhang Xiaoya Huang Chao Shen Ke Xu Li Zhou Yu Chen State Key Laboratory of Virology RNA InstituteCollege of Life Sciences and Frontier Science Center for Immunology and MetabolismWuhan UniversityWuhan430072China Institute for Vaccine Research at Animal Bio-safety Level III Laboratory Wuhan University School of MedicineWuhan430071China

The severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)is still epidemic around the *** manipulation of SARS-CoV-2 is restricted to biosafety level 3 laboratories(BSL-3).In this study,we developed a SARS-CoV-2ΔN-GFP-HiBiT replicon delivery particles(RDPs)encoding a dual reporter gene,GFP-HiBiT,capable of producing both GFP signal and luciferase *** optimal selection of the reporter gene,GFP-HiBiT demonstrated superior stability and convenience for antiviral ***,we established a RDP infection mouse model by delivering the N gene into K18-hACE2 KI mouse through *** mouse model supports RDP replication and can be utilized for in vivo antiviral *** summary,the RDP system serves as a valuable tool for efficient antiviral screening and studying the gene function of ***,this system can be manipulated in BSL-2 laboratories,decreasing the threshold of experimental requirements.

关键词： SARS-CoV-2 RDP Antiviral evaluation Mouse model BSL-2

The novel roles of ATP in modulating functions of sperm in ***

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The novel roles of ATP in modulating functions of sperm in *...

Efficient BoPDS Gene Editing in Cabbage by the CRISPR/Cas9 System

第五届中国秀丽线虫大会

作者： Xia Wang Qiushi Wang Ruijun He Long Miao Laboratory of Noncoding rna Institute of BiophysicsChinese Academy of Sciences

＜正＞Adenosine 5’-triphosphate（ATP）,as energy molecule or signaling molecule,plays multifunctional roles in various cellular activities,such as cell migration,neutrophils chemotaxis ***,the scenario about roles of ATP in nematode sperm cell remains *** we study functions of ATP in *** sperm and show that ATP is stored in membranous organelles（MOs）,

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Horticultural Plant Journal 2019年第4期5卷 164-169页

作者： Cunfa Ma Mengci Liu Qinfei Li Jun Si Xuesong Ren Hongyuan Song Key Laboratory of Horticulture Science for Southern Mountains Regions Ministry of EducationCollege of Horticulture and Landscape ArchitectureSouthwest University

Genome editing offers great advantages in identifying gene function and generating agronomically important mutations in crops. Here,we report that the genome of cabbage(Brassica oleracea var. capitata), an important cruciferous vegetable, can also be precisely edited by a CRISPR/Cas9 system stacked with multiple single-guide rna-expressing cassettes. When the phytoene desaturase Bo PDS gene was used as the target gene, an albino-phenotype transgenic shoot in T0 Basta-resistant lines was observed, and 37.5% of the transgenic cabbage shoots carried Bo PDS gene mutations as a result of nucleotide deletions at the expected position. Moreover, mutations were detected in sites with the same target sequence in gene Bol016089 which is paralogous to the Bo PDS gene. Our results show that the CRISPR/Cas9 system is a powerful tool for cabbage variety improvement by genome editing.

关键词： Cabbage CRISPR/Cas9 BoPDS Genome editing Mutation

Large-scale long terminal repeat insertions produced a significant set of novel transcripts in cotton

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Science China(Life Sciences) 2023年第8期66卷 1711-1724页

作者： Yan Yang Xingpeng Wen Zhiguo Wu Kun Wang Yuxian Zhu Institute for Advanced Studies Wuhan UniversityWuhan 430072China College of Life Sciences Wuhan UniversityWuhan 430072China Hubei Hongshan Laboratory Wuhan 430072China TaiKang Center for Life and Medical Sciences RNA InstituteRemin HospitalWuhan UniversityWuhan 430072China

Genomic analysis has revealed that the 1,637-Mb Gossypium arboreum genome contains approximately 81%transposable elements(TEs),while only 57%of the 735-Mb *** genome is occupied by *** this study,we investigated whether there were unknown transcripts associated with TE or TE fragments and,if so,how these new transcripts were evolved and *** sequence depths increased from 4 to 100 G,a total of 10,284 novel intergenic transcripts(intergenic genes)were *** average,approximately 84%of these intergenic transcripts possibly overlapped with the long terminal repeat(LTR)insertions in the otherwise untranscribed intergenic regions and were expressed at relatively low *** of these intergenic transcripts possessed no transcription activation markers,while the majority of the regular genic genes possessed at least one such *** without transcription activation markers formed their+1 and-1 nucleosomes more closely(only(117±1.4)bp apart),while twice as big spaces(approximately(403.5±46.0)bp apart)were detected for genes with the activation *** analysis of 183 previously assembled genomes across three different kingdoms demonstrated systematically that intergenic transcript numbers in a given genome correlated positively with its LTR *** analysis revealed that genic genes originated during one of the whole-genome duplication events around 137.7million years ago(MYA)for all eudicot genomes or 13.7 MYA for the Gossypium family,respectively,while the intergenic transcripts evolved around 1.6 MYA,resultant of the last LTR *** characterization of these low-transcribed intergenic transcripts can facilitate our understanding of the potential biological roles played by LTRs during speciation and diversifications.

关键词： rna-seq intergenic transcripts long terminal repeat epigenetic modification nucleosomes

Insights from the degradation mechanism of cyclin D into targeted therapy of the cancer cell cycle

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Signal Transduction and Targeted Therapy 2021年第9期6卷 2607-2608页

作者： Maiwen Caudron-Herger Sven Diederichs Division of rna Biology&Cancer German Cancer Research Center(DKFZ)HeidelbergGermany Division of Cancer Research Department of Thoracic SurgeryMedical Center-University of FreiburgUniversity of FreiburgGerman Cancer Consortium(DKTK)-Partner Site FreiburgFreiburgGermany

The control of cell proliferation is an essential feature in development and tissue homeostasis and is prominently deregulated in *** publications in Nature now uncover the mechanism underlying the degradation of cycl... 详细信息

关键词： targeted homeostasis cancer

Control of Directional Macromolecular Trafficking Across Specific Cellular Boundaries:A Key to Integrative Plant Biology

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Journal of Integrative Plant Biology 2007年第8期49卷 1227-1234页

作者： BiaoDing AsukaItaya Department of Plant Cellular and Molecular Biology and Plant Biotechnology Center Ohio State University ColumbusOhio 43210USA The OSU RNA GroupOhio State UniversityColumbusOhio 43210USA MolecularCellular and Developmental Biology ProgramOhio State UniversityColumbusOhio 43210USA

There is now solid evidence that cell-to-cell trafficking of certain proteins and rnas plays a critical role in trans-cellular regulation of gene expression to coordinate cellular differentiation and development. Such trafficking also is critical for viral infection and plant defense. The mechanisms of trafficking remain poorly understood. Although some proteins may move between cells by diffusion, many proteins and rnas move in a highly regulated fashion. Regulation is likely achieved through interactions between distinct protein or rna motifs and cellular factors. Some motifs and factors have been identified. One of the major focuses for future studies is to identify all motifs and their cognate factors and further elucidate their roles in trafficking between specific cells. With increasing information from such studies, we should be able to develop an understanding of the mechanisms that regulate trafficking of various proteins and rnas across all and specific cellular boundaries. On the basis of such mechanistic knowledge, we can further investigate how the trafficking machinery has evolved to regulate developmental and physiological processes in a plant, how pathogens have co-evolved to use this machinery for systemic spread in a plant, and how plants use this machinery for counterdefense.

关键词： CAPRICE GLABRA3 KNOTTED-I phloem plasmodesmata protein trafficking rna trafficking SHORT-ROOT viral movementprotein viroid

Fungal endophytes:an untapped source of biocatalysts

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Fungal Diversity 2012年第3期54卷 19-30页

作者： Trichur S.Suryanarayanan Nagamani Thirunavukkarasu Meenavalli B.Govindarajulu Venkat Gopalan Vivekananda Institute of Tropical Mycology(VINSTROM) RKM VidyapithChennai 600004India Department of Plant Biology and Plant Biotechnology RKM Vivekananda CollegeChennai 600004India Department of Biochemistry and Center for rna Biology The Ohio State UniversityColumbusOH 43210USA

Horizontally transmitted endophytes are an ecological group of fungi that infect living plant tissues and survive in them without causing any disease *** as facets of the endophyte-plant symbiotic relationship are being uncovered,there is an increasing appreciation of the different growth substrates exploited by endophytes and the vast repertoire of secreted enzymes of these *** attributes exemplify the striking biodiversity of fungal endophytes and should motivate bioprospecting these organisms to identify novel biocatalysts that might help address challenges in medicine,food security,energy production and environmental quality.

关键词： Fungal enzymes Microbial bioprospecting Biodiversity