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Journal of Integrative Agriculture 2017年第4期16卷 892-899页

作者： XU Hao-ran HE Lin XIAO Wei SHEN Guang-mao Key laboratory of entomology and Pest control Engineering College of Plant Protection Southwest University Chongqing 400715 P.R.China

Chitin is an important content in the exoskeletons of arthropods, and its hydrolyzation is catalyzed by chitinases during the process of molting, thus, the chitinases are considered as ideal target to interfere the growth of arthropods. This study intends to clarify the characteristic of the chitinases during the development of Tetranychus cinnabarinus, and screen out important genes as potential control targets. The results showed that the total enzyme concentration of chitinases was significantly higher in larva, the first and second nymph than that in egg and adult. Base on the transcriptome data, six unigenes encoding chitinases were identified and their expression patterns in different developmental stages were detected. The expressions of TcCHIT1 and TcCHITIO showed high abundance during the molting process and their expression change during the developmental stages was consistent with the enzyme concentration. The full-length of these two genes were further cloned, and the structural characteristics of their proteins were analyzed by constructing the three-dimensional structure model. The results provide basic information to understand the characteristic of chitinases in T. cinnabarinus and might be considered as target for control.

关键词： Tetranychus cinnabarinus chitinase gene expression protein structure

Identification,Characterization, and Expression of P450 Gene Encoding CYP6BQ13v2 from the Red Flour Beetle,Tribolium castaneum (Herbst) (Coleoptera:Tenebrionidae)

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Agricultural Sciences in China 2009年第10期8卷 1210-1218页

作者： XU Yong-qiang WANG Jin-jun JIANG Hong-bo DOU Wei TANG Pei-an AN Feng-ming Key laboratory of entomology and Pest control Engineering College of Plant Protection Southwest University Chongqing 400716P.R. China

An allele of CYP6BQI3, named CYP6BQ 13v2 （GenBank accession no. FJ209361）, was isolated from the red flour beetle, Tribolium castaneum （Herbst）（Coleoptera： Tenebrionidae） by RT-PCR. The cDNA sequence of CYP6BQ13v2, 1 563 bp in length, contains an open reading frame of 1 554 nucleotides encoding a putative protein of 518 amino acid residues with a predicted molecular weight of 59.92 kDa and a theoretical pl of 7.60. The putative protein contains the classic hemebinding sequence motif F××G×××C×G （residues 456-465） conserved among all P450 enzymes as well as other characteristic motifs of all cytochrome P450s. It shares 98% identity with the previously published sequence of CYP6BQ13 （GenBank accession no. XP967146） from the T. castaneum genome project. Phylogenetic analysis of amino acid sequences from members of various P450 families indicated that there was closer phylogenetic relationship of CYP6BQ 13v2 with CYP302A1 and CYP307A1 mediating synthesis of the insect molting hormone, distant relationship with CYP6B1 metabolizing plant allelochemicals, CYP6D 1 linking to pyrethroid resistance and other members of CYP6 family. Expression test of the gene in the adults and immature stages of T. castaneum by quantitative real-time PCR revealed that CYP6BQ13v2 is expressed in all life stages investigated. The mRNA expression level in 1st instar larvae was 14.9- and 3.86-fold higher than those in pupae and adults, respectively. The CYP6BQ13v2 expression levels appeared in the order of 1st instar larvae, followed by 4th instar larvae, 7th instar larvae, adult, and pupae from high to low. The more bioinformation of CYP6BQ 13v2 was also analyzed.

关键词： Tribolium castaneum cytochrome P450 real-time PCR analysis bioinformatics

Characterization of the Purified Glutathione S-transferases from Two Psocids Liposcelis bostrychophila and ***

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Agricultural Sciences in China 2010年第7期9卷 1008-1016页

作者： DOU Wei XIAO Li-sha NIU Jin-zhi JIANG Hong-bo WANG Jin-jun Key laboratory of entomology and Pest control Engineering College of Plant Protection Southwest University Chongqing 400716 P.R. China

Glutathione S-transferases（GSTs） from Liposcelis bostrychophila Badonnel and ***（Enderlein）（Psocoptera：Liposcelididae） were purified by glutathione-agarose affinity chromatography,and characterized subsequently by their Michaelis-Menten kinetics toward the artificial substrates 1-chloro-2,4-dinitrobenzene（CDNB） and reduced glutathione（GSH）,*** specific activity of the purified GST toward CDNB was 2.3-fold higher in *** than in *** the specific activities of purified enzymes varied between the two species,the purification yields were ***-PAGE revealed one band at 23 kDa for both the *** of *** exhibited higher Michaelis-Menten constants（Km） but lower maximal velocity（Vmax） values than those of *** optimum pH for CDNB conjugation of *** and *** GSTs was 7.0 and 7.5,and optimum temperature was 35 and 40°C,*** kinetics showed that cibacron blue,curcumin,bromosulfalein,ethacrynic acid,and carbosulfan had excellent inhibitory effects on GSTs in both species,but the inhibitory effects of beta-cypermethrin,fenpropathrin,tetraethylthiuram disulfide,and diethyl maleate were not significant.

关键词： GSTs purification psocids xenobiotic compounds

Multiple insecticide resistance/susceptibility status of Culex quinquefasciatus,principal vector of bancroftian filariasis from filaria endemic areas of northern India

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Asian Pacific Journal of Tropical Medicine 2011年第6期4卷 426-429页

作者： Kaushal Kumar Abhay K Sharma Sarita Kumar Sunita Patel Manas Sarkar Chauhan LS Centre for Medical entomology and vector Management National Centre for Diseases Control22-Shamnath MargDelhi-110054India Regional Filariasis Training and Research Centre National Centre for Diseases ControlVaranasi-221010India

Objective:To understand the insecticide resistance status of Culex quinquefasciatus Say（Diptera: Culicidae）（***） to deltamethrin,cyfluthrin,permethrin,lambdacyhalothrin, DDT and malathion in filarial endemic areas of Uttar Pradesh,***:Insecticide susceptibility assays were performed on wild-caught adult female *** mosquitoes to deltamethrin（0.05%）,cyfluthrin（0.15%）,permethrin（0.75%）,lambdacyhalothrin （0.05%）,malathion（5.0%） and DDT（4.0%）,the discriminating doses recommended by the World Health Organisation（WHO）.Results:The data showed that *** is highly resistant to DDT and malathion;the mortality was 28.33%and 27.5%,respectively and incipient resistance to synthetic pyrethroids（deltamethrin,cyfluthrin,permethrin,and lambdacyhalothrin）, where mortality ranged from 95.83%in permethrin to 98.33%in cyfluthrin and lambdacyhalothrin. Knockdown times(KDT50) in response to synthetic pyrethroids varied significantly between different insecticides（P<0.01） from 31.480 min for permethrin to 21.650 for cyfluthrin. Conclusions:The results presents here provide the status report of the insecticide resistance/ susceptibility of *** in major filaria endemic areas of northern India.

关键词： Insecticide resistance Filaria DDT Synthetic pyrethroid Malathion Culex quinquefasciatus

Complex interactions among insect viruses-insect vector-arboviruses

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Insect Science 2024年第3期31卷 683-693页

作者： Hui Wang Qian Chen Taiyun Wei State Key laboratory of Ecological Pest control for Fujian and Taiwan Crops Vector-borne Virus Research CenterFujian Agriculture and ForestryUniversityFuzhouChina

Insects are the host or vector of diverse viruses including those that infect vertebrates,plants,and *** viruses reside inside their insect hosts and are vertically transmitted from parent to *** insect virus-host relationship is intricate,as these viruses can impact various aspects of insect biology,such as development,reproduction,sex ratios,and ***-borne viruses(arboviruses)that cause substantial global health or agricultural problems can also be vertically transmitted to insect vector *** infections with insect viruses and arboviruses are common in *** coinfections involve complex interactions,including synergism,dependence,and *** studies have shed light on the influence of insect viruses on the competence of insect vectors for *** this review,we focus on the biological effects of insect viruses on the transmission of arboviruses by *** also discuss the potential mechanisms by which insect viruses affect the ability of hosts to transmit arboviruses,as well as potential strategies for disease control through manipulation of insect *** of the interactions among insect vectors,insect viruses and arboviruses will provide new opportunities for development of innovative strategies to control arbovirus transmission.

关键词： arbovirus insect biology insect vectors insect virus interaction transmission

Some ecological attributes of malarial vector Anopheles superpictus Grassi in endemic foci in southeastern Iran

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Asian Pacific Journal of Tropical Biomedicine 2013年第12期3卷 1003-1008页

作者： Jalil Nejati Hasan Vatandoost Mohammad Ali Oshghi Masud Salehi Ehssan Mozafari Seyed Hasan Moosa-Kazemi Department of Medical entomology & vector control School of Public HealthTehran University of Medical Sciences Malaria vector control Manager Centre for Disease Control & PreventionZahedan University of Medical Sciences Research Center for Infectious Diseases and Tropical Medicine Zahedan University of Medical Sciences

Objective:To determine the bionomics and susceptibility status of the malarial vector Anopheles superpictus(***)to different insecticides in the Sistan-Baluchestan province which has the highest malarial prevalence in ***:Different sampling methods,in addition to scoring abdominal conditions,were used to define the seasonal activity and endo/exophilic behavior of this *** addition,the standard WHO susceptibility tests were applied on adult field ***:Most adult mosquitoes were collected from outdoor *** peak of seasonal activity of *** occurred at the end of *** larvae were collected from natural and permanent breeding places with full sunlight and no *** feeding activities occurred around *** with the abdominal conditions of adult mosquitoes collected indoors,the abdominal conditions of adult mosquitoes collected outdoors were gravid and *** species was suspected to be resistant to DDT,but was susceptible to other ***:*** was present in almost all outdoor shelters,and the ratios of gravid,semigravid/unfed,and freshly fed confirmed that this species had a higher tendency to rest outdoors than *** behavior can protect *** from indoor residual spraying in this malarious *** the best of our knowledge,this is the first report on the susceptibility status of *** in Southeastern *** do not suggest the use of DDT for indoor residual spraying in southeast Iran.

关键词： Anopheles superpictus Malaria Iran

Ecdysteroids in the larvae of Opogona sacchari(Bojer),a invasive alien pest

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Chinese Science Bulletin 2007年第2期52卷 205-208页

作者： WANG FangHai ZHANG GuRen WEN RuiZhen HE GuoFeng State Key laboratory for Biological control & Institute of entomology Sun Yat-sen University Guangzhou 510275 China

In order to understand the composition and quantitative variation of ecdysteriods in the larvae of Opogona sacchari (Bojer), a invasive alien pest, we analyzed the larval ecdysteroid composition and titers in this pest. The main component of ecdysteriods in the larvae of O. sacchari is 20-hydroxyecdysone, and also there is a little 26-hydroxyecdysone. The titer of ecdysteriods in the larvae from the 1st instar to the 7th instar was gotten higher gradually compared on ng ecdyster-oid/larva, but no regularity could be found about the titer if compared on ng ecdysteroid/g avoirdupois. There was only one peak of ecdysteroids (0.5475 ng/larva) showed at day 2 during the developmental time of the 6th instar larvae. However, there were two peaks appearing during the developmental time of the 7th instar larvae , one peak (0.29415 ng/larva) at day 3, another (0.214 ng/larva) at day 5.

关键词：外来生物幼虫害虫植物

Apoptosis of Spodoptera litura larval hemocytes induced byheavy metal zinc

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Chinese Science Bulletin 2005年第24期50卷 2856-2860页

作者： XIA Qiang SUN Hongxia HU Xinjun SHU Yinghua GU Dexiang ZHANG Guren State Key laboratory for Biological control & Institute of entomology Sun Yat-sen University Guangzhou 510275 China

By adding different amount of zinc into the artificial medium of the insect larvae, the zinc-induced apoptosis of the larvae haemocytes of the herbivorous insect Spodoptera litura Fabricius was investigated with flow cy- tometer. The results showed that the increase of zinc dose in the artificial feed led to the accumulations of zinc in the lar- val hemolymph and fat body, and more zinc was accumu- lated in fat body than in hemolymph. The apoptosis of hemocytes was significantly induced at high zinc concentra- tion (1000 mg·kg?1) in the insect diet, and the apoptosis rate was 63.63%, which was remarkably higher than that at con- trol and lower concentrations (50―500 mg·kg?1). This sug- gests that the high dose of zinc in the artificial diet of S. litura larvae could induce the apoptosis of the larval hemocytes of S. litura.

关键词：重金属污染锌草地夜蛾幼虫血细胞细胞凋亡

Ecdysteroids in the pupae of Opogona sacchari (Bojer), a new invasive alien pest

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Chinese Science Bulletin 2006年第11期51卷 1324-1327页

作者： WANG Fanghai ZHANG Guren WEN Ruizhen HE Guofeng State Key laboratory for Biological control & Institute of entomology Sun Yat-sen University Guangzhou 510275 China

In order to understand the composition and quantitative variation of ecdysteriods in the pu- pae of Opogona sacchari (Bojer), a new invasive alien pest, we analyzed the pupal ecdysteroid com- position and titers in this pest. The main component of ecdysteriods in the pupae of O. sacchari is 20-hydroxyecdysone. The titre of ecdysteriods in both male pupae and female pupae get higher gradually, and then decreased slowly after reaching a peak (80 ng/pupa). The titre of ecdysteriods was about 30 ng/pupa in the later stage of pupae. The peak of male pupae appeared on the third day, but the peak of female pupae lasted from the second day to the fourth day during pupal developments. The ecdysteroid content was higher in female pupae than in male pupae during specific pupal time (the 1st, 2nd, 4th, 5th, 6th day). We also found that the titre of ec- dysteroids in posterior part of pupae was higher than in anterior part of pupae obviously.

关键词：蜕皮甾体蛹入侵有害物 Opogona sacchari

Approach from the laboratory to the field: New strategies in the control of mosquito-borne infectious diseases

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Science China Life Sciences 2025年第1期 303-305页

作者： Ziqian Xu Lianpan Dai Jun Liu National Key laboratory of Intelligent Tracking and Forecasting for Infectious Diseases (NITFID) National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and Prevention CAS Key laboratory of Pathogen Microbiology and Immunology Institute of MicrobiologyChinese Academy of Sciences

vector-borne viruses, transmitted among humans and animal hosts through bites of vectors such as mosquitoes and ticks,pose a great and ongoing threat to public health and biosafety(Yang et al., 2022).Among them, dengue virus-induced epidemics, have dramatically increased worldwide in recent decades. The report released by the World Health Organization(WHO) at the end of 2023 showed that dengue epidemic globally surged in2023, with the number of reported cases nearing a historical peak.

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