Characterization of the Purified Glutathione S-transferases from Two Psocids Liposcelis bostrychophila and ***

作     者：DOU Wei XIAO Li-sha NIU Jin-zhi JIANG Hong-bo WANG Jin-jun 

作者机构：Key Laboratory of Entomology and Pest Control Engineering College of Plant Protection Southwest University Chongqing 400716 P.R. China 

出 版 物：《Agricultural Sciences in China》 (中国农业科学（英文版）)

年 卷 期：2010年第9卷第7期

页      面：1008-1016页

核心收录：

学科分类：0710[理学-生物学] 090504[农学-特种经济动物饲养（含：蚕、蜂等）] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 0905[农学-畜牧学] 09[农学] 0901[农学-作物学] 0902[农学-园艺学] 

基　　金：funded in part by the National Natural Sciences Foundation of China (30871631) the Specialized Research Fund for the Doctoral Program of Higher Education of China to Prof. Wang Jinjun(200806350009) the Doctoral Program of Southwest University of China (SWU109023) the Fundamental Research Funds for the Central Universities,China(XDJK2009C112) 

主　　题：GSTs purification psocids xenobiotic compounds 

摘      要：Glutathione S-transferases（GSTs） from Liposcelis bostrychophila Badonnel and ***（Enderlein）（Psocoptera：Liposcelididae） were purified by glutathione-agarose affinity chromatography,and characterized subsequently by their Michaelis-Menten kinetics toward the artificial substrates 1-chloro-2,4-dinitrobenzene（CDNB） and reduced glutathione（GSH）,*** specific activity of the purified GST toward CDNB was 2.3-fold higher in *** than in *** the specific activities of purified enzymes varied between the two species,the purification yields were ***-PAGE revealed one band at 23 kDa for both the *** of *** exhibited higher Michaelis-Menten constants（Km） but lower maximal velocity（Vmax） values than those of *** optimum pH for CDNB conjugation of *** and *** GSTs was 7.0 and 7.5,and optimum temperature was 35 and 40°C,*** kinetics showed that cibacron blue,curcumin,bromosulfalein,ethacrynic acid,and carbosulfan had excellent inhibitory effects on GSTs in both species,but the inhibitory effects of beta-cypermethrin,fenpropathrin,tetraethylthiuram disulfide,and diethyl maleate were not significant.