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Inhibiting ceramide synthase 5 expression in microglia decreases neuroinflammation after spinal cord injury

Neural Regeneration Research 2025年第10期20卷 2955-2968页

作者： Wei Zhang Yubao Lu Ruoqi Shen Yingjie Wu Chenrui Liu Xingxing Fang Liangming Zhang Bin Liu Limin Rong Department of Spine Surgery The Third Affiliated HospitalSun Yat-sen UniversityGuangzhouGuangdong ProvinceChina National Medical products Administration(NMPA)Key Laboratory for quality Research and Evaluation of Cell products GuangzhouGuangdong ProvinceChina Guangdong Provincial Center for quality control of Minimally Invasive Spine Surgery GuangzhouGuangdong ProvinceChina Guangdong Provincial Center for Engineering and Technology Research of Minimally Invasive Spine Surgery GuangzhouGuangdong ProvinceChina

Microglia,the resident monocyte of the central nervous system,play a crucial role in the response to spinal cord ***,the precise mechanism remains *** investigate the molecular mechanisms by which microglia regulate the neuroinflammatory response to spinal cord injury,we performed single-cell RNA sequencing dataset analysis,focusing on changes in microglial *** found that the MG1 subpopulation emerged in the acute/subacute phase of spinal cord injury and expressed genes related to cell pyroptosis,sphingomyelin metabolism,and neuroinflammation at high ***,we established a mouse model of contusive injury and performed intrathecal injection of siRNA and molecular inhibitors to validate the role of ceramide synthase 5 in the neuroinflammatory responses and pyroptosis after spinal cord ***,we established a PC12-BV2 cell co-culture system and found that ceramide synthase 5 and pyroptosis-associated proteins were highly expressed to induce the apoptosis of neuron *** ceramide synthase 5 expression in a mouse model of spinal cord injury effectively reduced ***,ceramide synthase 5-induced pyroptosis was dependent on activation of the NLRP3 signaling *** ceramide synthase 5 expression in microglia in vivo reduced neuronal apoptosis and promoted recovery of neurological ***2g7 formed a“bridge”between sphingolipid metabolism and ceramide synthase 5-mediated cell death by inhibiting the NLRP3 signaling ***,these findings suggest that inhibiting ceramide synthase 5 expression in microglia after spinal cord injury effectively suppressed microglial pyroptosis mediated by NLRP3,thereby exerting neuroprotective effects.

关键词： ceramide synthase 5 gasdermin D microglia neuroinflammation NLRP3 nuclear factor kappa B Pla2g7 pyroptosis sphingomyelin metabolism spinal cord injury

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Avian influenza virus surveillance in migratory birds in Egypt revealed a novel reassortant H6N2 subtype

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Avian Research 2019年第4期10卷 507-516页

作者： Ali M.Zanaty Ahmed M.Erfan Wessam H.Mady Fatma Amer Ahmed A.Nour Neveen Rabie Mohamed Samy Abdullah A.Selim Wafaa M.M.Hassan Mahmoud M.Naguib National Laboratory for Veterinary quality control on poultry Production Animal Health Research InstituteGiza 12618Egypt Zoonosis Science Centre Department of Medical Biochemistry and MicrobiologyUppsala University75123 UppsalaSweden

Background:Avian influenza viruses(AIVs)have been identified from more than 100 different species of wild birds around the *** migratory birds can act as potential spreaders for AIVs to domestic birds between different *** is situated on important migratory flyways for wild birds between different *** much is known about circulation of zoonotic potential H5N1 and H9N2 AIVs in domestic poultry in Egypt,little is known about the pivotal role of migratory birds in the maintenance and transmission of the viruses in ***:Targeted AIV surveillance has been conducted in 2017 in different wetlands areas in Northern and Eastern ***:AIV of subtype H5 was detected in two bird *** addition,a novel reassortant strain of the H6N2 subtype was identified which reveals the continuous risk of new influenza virus(es)introduction into *** novel virus possesses a reassortant pattern originating from different AIV gene ***:Intervention control strategies should be performed to minimize the possible contact of domestic birds with wild birds to lower the risk of virus transmission at this *** addition,constant monitoring of AIVs in migratory birds is essential in the early detection of influenza virus introduction into Egypt.

关键词： Avian in fluenza virus H6N2 Egypt Reassortment Wild birds

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Effects of 105 traditional Chinese medicines on the detection ofβ-agonists in medicine extracts and swine urine based on colloidal gold immunochromatographic assay

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Journal of Integrative Agriculture 2021年第6期20卷 1626-1635页

作者： WANG Zi-lin FENG Ke-ying GE Xiu-feng MAI Jia-cheng WANG Han-chuan LIU Wen-zi ZHANG Jia-hui SHEN Xiang-guang Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation South China Agricultural UniversityGuangzhou 510642P.R.China animal and poultry products quality control Inspection and Testing CenterMinistry of Agriculture and Rural AffairsGuangzhou 510642P.R.China

Colloidal gold immunochromatographic assay(CGIA)is commonly used for the on-site detection ofβ-agonists that are sometimes used illegally as feed additives in swine ***,few studies have evaluated the causes of false-positive results that sometimes occur when applying CGIA in agricultural *** this study,we investigated if this false-positive phenomenon is related to the addition of certain traditional Chinese medicines(TCMs)to swine *** established and verified an extraction method for TCMs,and then applied CGIA to detectβ-agonists in the extracts of 105 TCMs and in the urine of swine dosed with TCMs,*** chromatography-tandem mass spectrometry was used to validate the results of the urine samples tested positive forβ-agonists using *** results were also verified using TCMs and colloidal gold test strips produced by different *** extracts of Citri Reticulatae Pericarpium Viride,Citri Reticulatae Pericarpium,Magnoliae Officinalis Cortex,Chaenomelis Fructus,and Rhodiolae Crenulatae Radix Et Rhizoma were tested positive forβ-***,the addition of Citri Reticulatae Pericarpium Viride and Citri Reticulatae Pericarpium to swine feed resulted in false-positive results forβ-agonists in swine *** results provide a new way to explain false-positive CGIA results and provide valuable information for livestock feeding programs.

关键词： colloidal gold immunochromatographic assay false positive traditional Chinese medicine β-agonists swine urine

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Insights into the mechanism of L-malic acid on drip loss of chicken meat under commercial conditions

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Journal of animal Science and Biotechnology 2024年第3期15卷 1312-1324页

作者： Haijun Sun Xue Yan Lu Wang Ruimin Zhu Meixia Chen Jingdong Yin Xin Zhang State Key Laboratory of animal Nutrition and Feeding College of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193China New Hope Liuhe Co. Ltd./Key Laboratory of Feed and Livestock and Poultry Products Quality&Safety ControlMinistry of AgricultureChengduSichuan 610023China Institute of animal Husbandry and Veterinary Medicine Beijing Academy of Agriculture and Forestry SciencesBeijing 100097China

Background A deterioration in the meat quality of broilers has attracted much more attention in recent years.L-malic acid(MA)is evidenced to decrease meat drip loss in broilers,but the underlying molecular mechanisms are still ***’s also not sure whether the outputs obtained under experimental conditions can be obtained in a com-mercial ***,we investigated the effects and mechanisms of dietary MA supplementation on chicken meat drip loss at large-scale *** Results showed that the growth performance and drip loss were improved by MA *** metabolome revealed that L-2-aminoadipic acid,β-aminoisobutyric acid,eicosapentaenoic acid,and nicotinamide,as well as amino acid metabolism pathways connected to the improvements of meat quality by MA *** transcriptome analysis further indicated that the effect of MA on drip loss was also related to the proper immune response,evidenced by the enhanced B cell receptor signaling pathway,NF-κB signaling pathway,TNF signaling pathway,and IL-17 signaling *** We provided evidence that MA decreased chicken meat drip loss under commercial *** and transcriptome revealed a comprehensive understanding of the underlying ***,MA could be used as a promising dietary supplement for enhancing the water-holding capacity of chicken meat.

关键词： Drip loss Immune response L-malic acid Meat quality Metabolome Transcriptome

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Dietary nano-selenium alleviated intestinal damage of juvenile grass carp(Ctenopharyngodon idella)induced by high-fat diet:Insight from intestinal morphology,tight junction,inflammation,anti-oxidization and intestinal microbiota

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animal Nutrition 2022年第1期8卷 235-248页

作者： Sha Liu Haibo Yu Pengju Li Chi Wang Guohao Liu Xiaotian Zhang Cheng Zhang Meng Qi Hong Ji College of animal Science and Technology Northwest A&F UniversityYangling712100China Key Laboratory of Se-enriched products Development and quality control Ministry of Agriculture and Rural AffairsAnkang725000China

In recent years,high-fat diet(HFD)has been widely applied in aquaculture,which reduces the intestinal health of cultured *** current study evaluated the protective effects of nano-selenium(nano-Se)on intestinal health of juvenile grass carp(Ctenopharyngodon idella)fed with HFD.A total of 135 experimental fish were fed with a regular diet(Con),a HFD(HFD)and a HFD containing nano-Se at 0.6 mg/kg(HSe)for 10 *** results showed that dietary nano-Se significantly improved the survival rate and feed efficiency which were reduced by HFD in juvenile grass carp(P<0.05).Also,nano-Se(0.6 mg/kg)supplement alleviated intestinal damage caused by the HFD,thus maintaining the integrity of the ***,it significantly up-regulated the expression of genes related to tight junction(ZO-1,claudin-3 and occludin),anti-oxidization(GPx4a and GPx4b),and the protein of ZO-1 in the intestine of juvenile grass carp,which were depressed by the HFD(P<0.05).Furthermore,nano-Se supplementation significantly suppressed the expressions of genes related to the inflammation,including inflammatory cytokines(IL-8,IL-1β,IFN-γ,TNF-αand IL-6),signaling molecules(TLR4,p38 MAPK and NF-kB p65),and protein expression of NF-kB p65 and TNF-αin the intestine of juvenile grass carp which were induced by the HFD(P<0.05).Besides,dietary nano-Se normalized the intestinal microbiota imbalance of juvenile grass carp caused by the HFD through increasing the abundance of the beneficial bacteria,e.g.,***,dietary nano-Se increased the production of short chain fatty acids(SCFA)in the intestine,especially for butyric acid and caproic acid,which were negatively related to the increase of intestinal permeability and *** summary,supply of nano-Se(0.6 mg/kg)in HFD could effectively alleviate intestinal injury of juvenile grass carp by improving intestinal barrier function and reducing intestinal inflammation and oxidative *** positive effects may be due to the regulatio

关键词： Nano-Se High-fat diet Inflammation Intestinal microbiota Short chainfattyacid

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Early inoculation with caecal fermentation broth alters small intestine morphology,gene expression of tight junction proteins in the ileum,and the caecal metabolomic profiling of broilers

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Journal of animal Science and Biotechnology 2020年第2期11卷 440-452页

作者： Yujie Gong Wenrui Xia Xueting Wen Wentao Lyu Yingping Xiao Hua Yang Xiaoting Zou Key Laboratory for Molecular animal Nutrition of Ministry of Education Feed Sciences InstituteCollege of Animal SciencesZhejiang University(Zijingang Campus)Hangzhou 310058China State Key Laboratory for quality and Safety of Agro-products Institute of Quality and Standards for Agro-productsZhejiang Academy of Agricultural SciencesHangzhou 310021China

Background:The establishment of stable microbiota in early life is beneficial to the *** in the intestinal environment during early life play a crucial role in modulating the gut ***,early intervention to change the intestinal environment can be regarded as a new regulation strategy for the growth and health of ***,the effects of intestinal environmental changes on host physiology and metabolism are rarely *** study was conducted to investigate the effects of early inoculation with caecal fermentation broth on small intestine morphology,gene expression of tight junction proteins in the ileum,and cecum microbial metabolism of ***:Our data showed that early inoculation with caecal fermentation broth could improve intestine *** small intestine villus height was significantly increased(Pcontrol group,especially on day 28.A similar result was observed in the ratio of villus height to crypt depth(Pcontrol group(C group).The differentially expressed metabolites were identified,and they were mainly functionally enriched in beta-alanine metabolism and biosynthesis of unsaturated fatty *** addition,1,3-diaminopropane was selected as a key biomarker that responded to early inoculation with caecal fermentation ***:These re

关键词： Broilers Early inoculation Gene expression Intestine morphology Metabolomics profiling

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Ruminal bacterial community is associated with the variations of total milk solid content in Holstein lactating cows

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animal Nutrition 2022年第2期9卷 175-183页

作者： Kaizhen Liu Yangdong Zhang Guoxin Huang Nan Zheng Shengguo Zhao Jiaqi Wang State Key Laboratory of animal Nutrition Institute of Animal SciencesChinese Academy of Agricultural SciencesBeijing 100193China Key Laboratory of quality&Safety control for Milk and Dairy products of Ministry of Agriculture and Rural Affairs Institute of Animal SciencesChinese Academy of Agricultural SciencesBeijing 100193China

Total milk solid(TMS)content directly reflects the quality of *** bacteria ferment dietary components,the process of which generates the precursors for the synthesis of milk solid,therefore,the variation in rumen bacterial community could be associated with milk solid in dairy *** this study,45 healthy mid-lactation Holstein dairy cows with the similar body weight,lactation stage,and milk yield were initially used for the selection of 10 cows with high TMS(HS)and 10 cows with low TMS(LS).All those animals were under the same feeding management,and the individual milk yield was recorded for 14 consecutive days before milk and rumen fluid were *** fluid was used to determine bacterial community by 16S rRNA gene sequencing *** HS cows had significantly greater feed intake and milk TMS,fat,protein content than LS cows(P0.05),nor was the acetate-to-propionate ratio,pH value,ammonia nitrogen and microbial crude protein concentrations(P>0.05).Significant differences in the relative abundances of some bacterial genera were found between HS and LS ***’s rank correlation analysis revealed that TMS content was correlated positively with the abundances of Ruminococcaceae UCG-014,Ruminococcaceae NK4A214 group,Prevotellaceae UCG-001,Butyrivibrio 2,Prevotellaceae UCG-003,Candidatus Saccharimonas,Ruminococcus 2,Lachnospiraceae XPB1014 group,probable genus 10,Eubacterium ventriosum group,but negatively correlated with *** addition,Ruminococcaceae UCG-014,Ruminococcus 2,Ruminococcaceae UCG001,probable genus 10 and Eubacterium ventriosum group might boost the total VFA production in the *** conclusion,the dry matter intake of dairy cows and some special b

关键词： Dairy cow Total milk solid content Rumen bacteria Milk fat

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New strategy for COVID-19 vaccination:targeting the receptor-binding domain of the SARS-CoV-2 spike protein

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Cellular & Molecular Immunology 2021年第2期18卷 243-244页

作者： Junzhi Wang Key Laboratory of the Ministry of Health for Research on quality and Standardization of Biotech products National Institutes for Food and Drug ControlBeijingChina

The unexpected outbreak of SARS-CoV-2 infection,with over 42.5 million confirmed cases and more than 1.1 million deaths globally,^(1)has posed a great threat to public *** global efforts to control this viral infection,the pandemic is still *** facing such a serious,unprecedented situation,vaccines are likely the most promising method to control this newly identified coronavirus.A recent study from Yang et al.^(2)showed that a recombinant spike receptor-binding domain(RBD)protein of SARS-CoV-2 prepared from insect cells induced protective immunity in nonhuman primates,providing a feasible vaccine candidate for COVID-19 control(Fig.1).

关键词： protective immunity vaccine

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A bivalent recombinant vaccine:a promising strategy against both SARS-CoV-2 variants and wild type of the virus

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Signal Transduction and Targeted Therapy 2021年第8期6卷 2266-2267页

作者： Junzhi Wang Key Laboratory of the Ministry of Health for Research on quality and Standardization of Biotech products National Institutes for Food and Drug ControlBeijingChina

A recent study published in Medcomm by He et al.1 introduced protein subunit vaccines with wild-type and mutant S1 subunit(S1-WT and S1-Mut)of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)to evaluate their immunoprotective *** team further analyzed the neutralization activity of bivalent vaccine composed of S1-WT and S1-Mut for SARS-CoV-2 and mutant strains to explore a universal protective vaccine.

关键词： protective vaccine

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Effects of different ratios of soluble to insoluble dietary fiber on growth performance and intestinal health of piglets

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animal Nutrition 2024年第3期18卷 257-271页

作者： Luya Feng Zhenfu Luo Jing Wang Kunfu Wu Wenliang Wang Zhimou Liu Juping Wen Zhenbin Wang Gregory JDuns Xiaokang Ma Bi'e Tan Hunan Provincial Key Laboratory for the products quality Regulation of Livestock and poultry College of Animal Science and TechnologyHunan Agricultural UniversityChangsha 410128China Yuelushan Laboratory Changsha 410128China Hunan Nuoze Biological Technology Co. Ltd.Yiyang 413001China

This study investigated the impact of different ratios of soluble to insoluble dietary fiber(SDF:IDF)formulations by sugar beet pulp(SBP)supplementation on piglet growth performance,nutrient digestibility,immune function,intestinal morphology,intestinal microbiota and intestinal health.A total of 60 crossbred piglets(Duroc×[Landrace×Yorkshire])at 40 d old with body weight of 10.0±0.3 kg were randomly assigned to 5 treatments with 6 replicates per treatment and 2 piglets per replicate in a 21-d *** dietary treatments included a corn-soybean meal diet(0%SBP supplementation;CON),and diets supplemented with 2%,4%,6%,and 8%SBP,representing different SDF:IDF ratios at 10.16%,13.53%,16.79%,19.86%,and 24.81%,*** results indicated that the 8%SBP treatment had a negative effect on feed-to-gain ratio(linear,P=0.009)compared with the CON treatment(P=0.021).The apparent total tract digestibility(ATTD)of crude protein was lower in treatments supplemented with SBP(P=0.002)and showed a linear decrease(P=0.001),while the ATTD of IDF showed a linear increase(P=0.037)in four SBP treatments compared to the CON *** 4%SBP treatment increased serum concentrations of triglyceride(quadratic,P=0.019)and K(linear,P<0.0037),and decreased alanine transaminase concentration(quadratic,P=0.015)compared with the CON *** concentrations of Cit,Cys,Ile,Leu,Orn,Arg,taurine,urea,1-methylhistidine,α-aminoadipic acid,α-aminobutyric acid and cystathionine in the 4%SBP treatment were highest among all treatments(P<0.05).The serum concentrations of interleukin-6,interleukin-8,interleukin-10,transforming growth factor-β,and tumor necrosis factor-αin the 6%SBP treatment were higher than those in the CON treatment(P<0.05),which also increased mucin-2 and G protein-coupled receptor 41 mRNA expression(P<0.05)in colonic mucosa compared with the CON treatment and improved the intestinal barrier *** containing more than 19.86%SDF:IDF could impair the intestinal healt

关键词： Soluble dietary fiber Insoluble dietary fiber Sugar beet pulp Growth performance Intestinal health Piglet

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