Effects of 105 traditional Chinese medicines on the detection ofβ-agonists in medicine extracts and swine urine based on colloidal gold immunochromatographic assay

作     者：WANG Zi-lin FENG Ke-ying GE Xiu-feng MAI Jia-cheng WANG Han-chuan LIU Wen-zi ZHANG Jia-hui SHEN Xiang-guang WANG Zi-lin;FENG Ke-ying;GE Xiu-feng;MAI Jia-cheng;WANG Han-chuan;LIU Wen-zi;ZHANG Jia-hui;SHEN Xiang-guang

作者机构：Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety EvaluationSouth China Agricultural UniversityGuangzhou 510642P.R.China Animal and Poultry Products Quality ControlInspection and Testing CenterMinistry of Agriculture and Rural AffairsGuangzhou 510642P.R.China 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2021年第20卷第6期

页      面：1626-1635页

核心收录：

学科分类：0710[理学-生物学] 090603[农学-临床兽医学] 0832[工学-食品科学与工程（可授工学、农学学位）] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 0905[农学-畜牧学] 0906[农学-兽医学] 09[农学] 0901[农学-作物学] 0703[理学-化学] 0902[农学-园艺学] 0713[理学-生态学] 

基　　金：the Guangdong Public Welfare Research and Capacity Building Project China(2015A020209130) 

主　　题：colloidal gold immunochromatographic assay false positive traditional Chinese medicine β-agonists swine urine 

摘      要：Colloidal gold immunochromatographic assay(CGIA)is commonly used for the on-site detection ofβ-agonists that are sometimes used illegally as feed additives in swine ***,few studies have evaluated the causes of false-positive results that sometimes occur when applying CGIA in agricultural *** this study,we investigated if this false-positive phenomenon is related to the addition of certain traditional Chinese medicines(TCMs)to swine *** established and verified an extraction method for TCMs,and then applied CGIA to detectβ-agonists in the extracts of 105 TCMs and in the urine of swine dosed with TCMs,*** chromatography-tandem mass spectrometry was used to validate the results of the urine samples tested positive forβ-agonists using *** results were also verified using TCMs and colloidal gold test strips produced by different *** extracts of Citri Reticulatae Pericarpium Viride,Citri Reticulatae Pericarpium,Magnoliae Officinalis Cortex,Chaenomelis Fructus,and Rhodiolae Crenulatae Radix Et Rhizoma were tested positive forβ-***,the addition of Citri Reticulatae Pericarpium Viride and Citri Reticulatae Pericarpium to swine feed resulted in false-positive results forβ-agonists in swine *** results provide a new way to explain false-positive CGIA results and provide valuable information for livestock feeding programs.