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Genes & Diseases 2024年第4期11卷 106-108页

作者： Gloria Garavito-De Egea Alex Dominguez-Vargas Jorg e l.Velez Gustavo Aroca Luis Fagng Elkin Navarro-Quiroz Zilac Espitaleta Kenny Del Toro-Camargo Leticia Martinez-Ariza Tatiana Gonzalez-Vargas Susana Garcia Mauricio Arcos-Burgos Eduardo Egea Universidad del Norte Division Ciencias de la SaludBarranquillaColombia Universidad Simon Bolivar Facultad de Ciencias de la SaludBarranquillaColombia Clinica de la costa Grupo de Investigacion en NefrologiaBarranquillaColombia Universidad de Antioquia Grupo de Investigacion en Psiquiatria(GIPSI)Departamento de PsiquiatriaInstituto de Investigaciones MedicasFacultad de MedicinaMedellinColombia

Genome-wide association studies(GWASs)have identified hundreds of loci across the human genome conferring susceptibility to autoimmune diseases(AIDs),some of which are shared between more than two ***,this univariate ... 详细信息

关键词： susceptibility diseases diabetes

Clinical Profiles at the Time of diagnosis of SARS-CoV-2 Infection in costa Rica During the Pre-vaccination Period Using a Machine Learning Approach

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Phenomics 2022年第5期2卷 312-322页

作者： Jose Arturo Molina-Mora Alejandra González Sergio Jiménez-Morgan Estela Cordero-Laurent Hebleen Brenes Claudio Soto-Garita Jorge Sequeira-Soto Francisco Duarte-Martínez Centro de Investigación en Enfermedades Tropicales(CIET)and Facultad de Microbiología Universidad de Costa RicaSan José2060Costa Rica Instituto costarricense de Investigación y Enseñanza en Nutrición y Salud(INCIENSA) Tres Ríos 30301Costa Rica Escuela de medicina Universidad de Costa RicaSan José2060Costa Rica

The clinical manifestations of COVID-19,caused by the SARS-CoV-2,define a large spectrum of symptoms that are mainly dependent on the human host *** costa Rica,more than 169,000 cases and 2185 deaths were reported during the year 2020,the pre-vaccination *** describe the clinical presentations at the time of diagnosis of SARS-CoV-2 infection in costa Rica during the pre-vaccination period,we implemented a symptom-based clustering using machine learning to identify clusters or clinical profiles at the population level among 18,974 records of positive *** were compared based on symptoms,risk factors,viral load,and genomic features of the SARS-CoV-2 sequence.A total of 18 symptoms at time of diagnosis of SARS-CoV-2 infection were reported with a frequency>1%,and those were used to identify seven clinical profiles with a specific composition of clinical *** the comparison between clusters,a lower viral load was found for the asymptomatic group,while the risk factors and the SARS-CoV-2 genomic features were distributed among all the *** other distribution patterns were found for age,sex,vital status,and *** conclusion,during the pre-vaccination time in costa Rica,the symptoms at the time of diagnosis of SARS-CoV-2 infection were described in clinical *** host co-morbidities and the SARS-CoV-2 genotypes are not specific of a particular profile,rather they are present in all the groups,including asymptomatic *** addition,this information can be used for decision-making by the local healthcare institutions(first point of contact with health professionals,case definition,or infrastructure).In further analyses,these results will be compared against the profiles of cases during the vaccination period.

关键词： COVID-19 costa Rica Machine learning diagnosis SARS-CoV-2 Clinical profiles

Understanding Ehrlichia canis Infections in Dogs of costa Rica: Hematological Findings and Indicative Clinical Signs

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Open Journal of Veterinary Medicine 2016年第11期6卷 163-175页

作者： Alexander V. Barrantes-González Ana E. Jiménez-Rocha Juan José Romero-Zuñiga Gaby Dolz Programa de Investigación en medicina Poblacional Escuela de Medicina Veterinaria Universidad Nacional Heredia Costa Rica Laboratorio de Parasitología Escuela de Medicina Veterinaria Universidad Nacional Heredia Costa Rica

Background: A cross-sectional study combining different serological and molecular techniques for the detection of Ehrlichia canis in dogs was carried out to determine hemopathological findings and suggestive clinical signs associated with acute, subclinical and chronic infections in the dog population of costa Rica. Objectives: The present study describes and analyzes, in a more representative sampling frame, the clinical and hematological presentation of E. canis infection in dogs of costa Rica in all its clinical stages. Methods: A descriptive analysis of the clinical signs was performed from a 441-dog sample. Serological and molecular techniques for the detection of Ehrlichia canis in dogs were applied. One and two-way ANOVA were carried out to determine the effect of the infection status on the hematological parameters. Results: A total of 0.7% (3/407) dogs were found with acute (seronegative but PCR positive), 29.7% (121/407) with subclinical (seropositive and PCR negative), and 2.5% (10/407) with chronic (seropositive and PCR positive) E. canis infections. Significant hemopathological findings were determined in dogs with acute (thrombocytosis), subclinical and chronic (anemia, thrombocytopenia, leukopenia) E. canis infections. Conclusions: Future studies must determine if dogs with subclinical E. canis infections eliminated the agent without any medication, or if they continue to be persistently infected, and will develop the chronic disease at some point in their lives.

关键词： Ehrlichiosis Epidemiology Hematopathology Infectious Disease Clinical Pathology

Persistence of Anti-SARS-CoV-2 IgM Antibody up to 8 Months Post-COVID-19

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Case Reports in Clinical Medicine 2021年第9期10卷 227-231页

作者： Carlos David A. Bichara Ednelza S. G. Amoras Gergiane L. Vaz Cléa N. C. Bichara Isabella P. C. amaral amaral Antonio C. R. Vallinoto Laboratório de Virologia Instituto de Ciências Biológicas Universidade Federal do Pará (UFPA) Belém Brasil amaral costa medicina diagnóstica Belém Brasil Faculdade de medicina Centro de Ciências Biológicas e da Saúde Universidade do Estado do Pará (UEPA) Belém Brasil Programa de Pós-Graduaç ã o em Biologia de Agentes Infecciosos e Parasitários Instituto de Ciências Biológicas Universidade Federal do Pará (UFPA) Belém Brasil

Here, we present a longitudinal analysis of two patients who recovered from COVID-19 more than 8 months prior but showed persistent serological detection of anti-SARS-CoV-2 IgM. We still do not know the exact reason for this prolonged persistence of IgM in these patients. To our knowledge, these are the first reports of IgM persistence in the context of SARS-CoV-2 infection and point to the need for no longer using IgM as a diagnostic criterion for acute or recent COVID-19. One should opt for gold standard molecular methodologies due to their high sensitivity and specificity.

关键词： COVID-19 SARS-CoV-2 Antibodies IgM Persistence

Prevalence of Psittacine Beak and Feather Disease Virus and Avian Polyomavirus in Captivity Psittacines from costa Rica

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Open Journal of Veterinary Medicine 2013年第4期3卷 240-245页

作者： Gaby Dolz Jessica Sheleby-Elías Juan J.Romero-Zuniga Bernardo Vargas-Leiton Gustavo Gutiérrez-Espeleta Kenneth Madriz-Ordenana Laboratorio de Entomología y medicina Poblacional Programa MEDPOBEscuela de Medicina VeterinariaUniversidad NacionalHerediaCosta Rica Maestría en Enfermedades Tropicales Posgrado Regional en Ciencias Veterinarias TropicalesEscuela de Medicina VeterinariaUniversidad NacionalHerediaCosta Rica Escuela de Biología Universidad de Costa RicaSan JoseCosta Rica Biotécnica Análisis Moleculares San JoseCosta Rica

Psittacine beak and feather disease virus (PBFDV) and avian polyomavirus (APV) are the most common viral diseases in psittacine birds, both affecting feathers and physical appearance of birds. Between 2005 and 2009, a total of 269 samples were collected from birds presented at veterinary clinics, shelters and rescue centers of wildlife in costa Rica. They belonged to 19 species of psittacine birds. The most representative species in the sample were Ara macao (157), Ara ambigua (37), Amazona autumnalis (24), Amazon ochrocephala (21) and Ara ararauna (8). A prevalence of 19.7% (53/269) for PBFDV and 4.8% (13/269) for APV was determined using Polymerase Chain Reaction (PCR). In 3.3% (9/269) of the birds mixed infections were detected. Statistical analysis determined that psittacines living in shelters and rescue centers had a greater risk to be positive to PBFDV and APV than birds that were presented at veterinary clinics, while only for PBFDV it was determined, that it is more likely to detect it in feathers than in blood. Finally, birds infected with PBFDV had 6.24 times more probability to become infected with APV, than non-infected birds. This is the first report of prevalence of PBFDV and APV in captive psittacines from costa Rica.

关键词： Psittacine Beak and Feather Disease Virus Avian Polyomavirus Psittacine Birds costa Rica

Evaluation of a Voluntary Control Program for the Detection of Bovine Leukemia Virus Antibodies Based on Agar Gel Immunodiffusion Test in Dairy Farms in costa Rica

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Open Journal of Veterinary Medicine 2015年第12期5卷 229-233页

作者： Gaby Dolz Francine Huijsen Carlos Jiménez Luis L. Rodríguez Escuela de medicina Veterinaria Universidad Nacional Heredia Costa Rica

Cattle from 20 dairy farms were serologically tested over a five-year period using agar gel immunodiffusion test (AGIDT) as part of a voluntary Bovine Leukemia Virus (BLV) control program. After five years of removing infected animals from the herds based on BLV-AGIDT serological status, blood samples from 332 cattle in these farms were collected and analyzed side by side by AGIDT and enzyme-linked immunosorbent assay (ELISA) to detect antibodies against BLV. AGIDT detected 29.2% (97) and 16.0% (53) of the animals as positive and weak positive respectively, whereas ELISA detected 58.2% (193) cattle as positive. The prevalence of BLV-antibodies determined with AGIDT in the dairy farms oscillated between 0% and 86%, whereas prevalence determined by ELISA ranged between 28% and 100% in the same farms. Although both techniques showed similarly results in farms with high BLV-prevalence, ELISA detected a larger proportion of BLV-positive, especially in farms with low or no BLV-prevalence based on AGIDT, leading to wrong assumptions in terms of farm level control efforts. Our results strongly suggest that AGIDT alone is inadequate to implement BLV control programs and ELISA is a more adequate test for BLV surveillance and control programs.

关键词： BLV Serological Testing Control Programs

Effect of Changes in Body Weight,Body Condition and Back Fat During Last Month of Pregnancy on the Reproductive Efficiency of Bos indicus Cows in the Tropics of costa Rica

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Open Journal of Veterinary Medicine 2013年第1期3卷 22-28页

作者： Jaime Galindo Carlos S.Galina Sandra Estrada Juan Jose Romero Marco Alarcon Martín Maquivar Escuela de Agronomía Instituto Tecnologico de Costa RicaAlajuelaCosta Rica Departamento de Reproduccion Facultad de Medicina Veterinaria y ZootecniaUniversidad Nacional Autonoma de MexicoMexico DFMexico Programa de Investigacion en medicina Poblacional Escuela de Medicina VeterinariaUniversidad Nacional HerediaCampus Benjamín NunezLagunillaCosta Rica Department of Animal Sciences The Ohio State UniversityColumbusUSA

With the aim of evaluating how changes in the metabolic status in the last month of pregnancy affects reproductive efficiency, forty six Bos indicus multiparous cows (5.5 ± 2.4 parturitions), were used. Measurements of body weight (BW), body condition score (BCS) and dorsal back fat (BF) were taken in the last month of pregnancy, postpartum period previous to synchronization (average 50 d) and at breeding by natural mating following synchronization with a progestin (average 70 d). Average postpartum days to resumption to ovarian activity were 79.96 ± 16.5 d, and average postpartum days to conception was 88.5 ± 14 d. Days postpartum to resumption of the ovarian activity was positively correlated (0.51, P 0.01) with days postpartum to conception, also, days postpartum to conception was positively correlated with prepartum back fat (14 d before parturition) (0.44, P 0.05). It was observed that BF at calving which is an objective measurement had a low correlation with other productive variables such as BCS and BW (always less than 0.39). Body fat might be a more reliable indicator of the current metabolic status of the animal particularly in the last month of pregnancy when the indicators of BCS are somehow more difficult to interpret and subjective.

关键词： Bos indicus Cattle Body Condition Body Weight Reproduction

Edaphoclimatic seasonal trends and variations of the Salmonella spp. infection in Northwestern Mexico

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Infectious Disease Modelling 2021年第1期6卷 805-819页

作者： Yasiri Mayeli Flores Monter Andrea Chaves Beatriz Arellano-Reynoso Andres Mauricio Lopez-Perez Humberto Suzan-Azpiri Gerardo Suzan Facultad de medicina Veterinaria y Zootecnia Universidad Nacional Autonoma de MexicoMexico City04510Mexico Escuela de Biología Universidad de Costa RicaSan Jose11501-2060Costa Rica Department of Medicine and Epidemiology School of Veterinary MedicineUniversity of CaliforniaDavisCA95695United States Laboratorio de Ecología Universidad Autonoma de Queretaro76230Mexico

Currently,Salmonella *** the bacterium causing the highest number of food-borne diseases(FADs)in the *** is primarily associated with contaminated water used to that irrigates crops from intensive livestock ***,literature emphasizes that the reservoirs for Salmonella *** in wildlife and there are unconventional sources or secondary reservoirs,such as *** soil-borne diseases have not been modeled in spatial scenarios,and therefore it is necessary to consider soil and other climatic factors to anticipate the emergence of new strains or serotypes with potential threat to public and animal *** objective of this research was to investigate whether edaphic and climatic factors are associated with the occurrence and prevalence of Salmonella *** Northwestern *** estimated the potential distribution of Salmonella *** an interpolation method of unsampled kriging areas for 15 environmental variables,considering that these factors have a seasonal dynamic of change during the year and modifications in longer ***,a database was generated with human salmonellosis cases reported in the epidemiological bulletins of the National System of Epidemiological Surveillance(SIVE).For the Northwest region,there were 30,595 human cases of paratyphoid and other salmonellosis reported have been reported in Baja California state,71,462 in Chihuahua,and 16,247 in Sonora from 2002 to *** highest prevalence was identified in areas with higher temperatures between 35 and 37℃,and precipitation greater than 1000 *** edaphic variables limited the prevalence and geographical distribution of Salmonella spp.,because the region is characterized by presenting a low percentage of organic matter(≤4.3),and most of the territory is classified as aridic and xeric,which implies that the humidity comprises≤180 days a ***,the seasonal time series indicated that in the states of Baja California and Chihuahua the rainy quarter of

关键词： Potential distribution Soil Temperature Precipitation Salmonella spp

Green Synthesis and Antibacterial Properties of Silver Nanoparticles from Eugenia uniflora Fruit Extract

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Advances in Nanoparticles 2023年第3期12卷 94-105页

作者： Melissa Moya Vanessa Bagnarello Julio Mora Idalia Valerio Laboratorio de Investigación Universidad de Ciencias Médicas San José Costa Rica Facultad de Microbiología Universidad de Ciencias Médicas San José Costa Rica Facultad de Fisioterapia Universidad de Ciencias Médicas San José Costa Rica Facultad de medicina Universidad de Ciencias Médicas San José Costa Rica

The synthesis of nanoparticles by biological methods using microorganisms, enzymes, or plant extracts has been suggested as a possible ecofriendly alternative to chemical and physical methods that involve the use of harmful reducing agents. Green synthesis of silver nanoparticles (AgNPs) was achieved using Eugenia uniflora ripe fruit extract, which was characterized by phytochemical screening revealing the presence of polyphenols (quinones, flavonoids, and tannins), reducing compounds, and terpenes. These excellent antioxidants reduced silver nitrate to give the AgNPs, which were characterized by transmission electron microscopy (TEM), dynamic light scattering (DLS), and ζ potential analysis. The diameter of the AgNPs ranged from 10.56 ± 1.2 nm to 107.56 ± 5.7 nm. The antibacterial activity of the AgNPs was evaluated using a modification of the Kirby-Bauer technique with Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus. The inhibition halos were 11.12 ± 0.02 mm, 13.96 ± 0.07 mm, and 11.29 ± 0.76 mm, respectively. The synthesis using E. uniflora is an ecofriendly and low cost method of obtaining silver nanoparticles that could be used in health sciences because of their activity against bacteria with antibiotic resistance.

关键词： Eugenia uniflora Green Synthesis Silver Nanoparticles Antibacterial Activity Polyphenols

Effect of Aspergillus niger prolyl endopeptidase in patients with celiac disease on a long-term gluten-free diet

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World Journal of Gastroenterology 2024年第11期30卷 1545-1555页

作者： Juan Pablo Stefanolo Verónica Segura Martina Grizzuti Abel Heredia Isabel Comino Ana Florencia costa Roberto Puebla María Paz Temprano Sonia Isabel Niveloni Gabriel de Diego María E Oregui Edgardo Gustavo Smecuol Mauricio C de Marzi Elena F Verdú Carolina Sousa Julio César Bai Small Bowel Section Department of MedicineGastroenterology Hospital of Buenos Aires“Dr.C.Bonorino Udaondo”Buenos Aires 1264Argentina Department of Microbiology and Parasitology Faculty of PharmacyUniversity of SevilleSeville 41080Spain Department of Medicine Dr.C.Bonorino Udaondo Gastroenterology HospitalBuenos Aires 1264Argentina Basic and Applied Research Group in Immunology and Bioactives(GIBAIB) Institute of Ecology and Sustainable Development(INEDES)National University of LujanLuján 6700Buenos AiresArgentina Department of Medicine Farncombe Family Digestive Health Research InstituteMcMaster UniversityHamilton L8S 4L8OntarioCanada Facultad de medicina Universidad del SalvadorBuenos Aires C1051ABBArgentina

BACKGROUND The gluten-free diet(GFD)has limitations,and there is intense research in the development of adjuvant *** To examine the effects of orally administered Aspergillus niger prolyl endopeptidase protease(AN-PEP)on inadvertent gluten exposure and symptom prevention in adult celiac disease(CeD)patients following their usual *** This was an exploratory,double-blind,randomized,placebo-controlled trial that enrolled CeD patients on a long-term *** a 4-wk run-in period,patients were randomized to 4 wk of two AN-PEP capsules(GliadinX;AVI Research,LLC,United States)at each of three meals per day or *** endpoints were:(1)Average weekly stool gluten immunogenic peptides(GIP)between the run-in and end of treatments and between AN-PEP and placebo;(2)celiac symptom index(CSI);(3)CeD-specific serology;and(4)quality of *** samples were collected for GIP testing by ELISA every Tuesday and Friday during run-ins and *** Forty patients were randomized for the intention-to-treat analysis,and three were excluded from the per-protocol ***,628/640(98.1%)stool samples were *** was undetectable(0.32μg/g)to potentially cause mucosal *** GIP concentration in the AN-PEP arm was 44.7%lower than in the run-in ***-third of patients exhibiting GIP>0.08μg/g during run-in had lower or undetectable GIP after AN-PEP *** with the run-in period,the proportion of symptomatic patients(CSI>38)in the AN-PEP arm was significantly lower(P<0.03).AN-PEP did not result in changes in specific *** This exploratory study conducted in a real-life setting revealed high adherence to the *** AN-PEP treatment did not significantly reduce the overall GIP stool ***,given the observation of a significantly lower prevale

关键词： Celiac disease Aspergillus niger prolyl endoprotease Gluten immunogenic peptides Trial Symptoms Real-life trial