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The Use of Anti-VDAC2 Antibody for the Combined Assessment of Human Sperm Acrosome Integrity and Ionophore A23187-Induced Acrosome Reaction

中华医学会生殖医学分会人类精子库管理学组第三届年会全国男性生殖医学和精子库管理新进展第四次研讨会

作者： Zenqjun Wang Jiangsu Province hospital Sperm bank

Purpose To analyze the abundance and difference of voltagedependent anion channel(VDAC) mRNA in ejaculated spermatozoa from normozoospermic fertile donors and infertile patients with idiopathic *** High motile and low...

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The Use of Anti-VDAC2 Antibody for the Combined Assessment o...

Procaine Inhibiting Human Bladder Cancer Cell Proliferation by Inducing Apoptosis and Demethylating APAF1 CpG Island Hypermethylated

中华医学会生殖医学分会人类精子库管理学组第三届年会全国男性生殖医学和精子库管理新进展第四次研讨会

作者： Zengjun Wang Jiangsu Province hospital Sperm bank

Voltage-dependent anion channel(VDAC) is mainly located in the mitochondrial outer membrane and participates in many biological *** mammals,three VDAC subtypes (VDAC1,2 and 3) have been *** VDAC has been extensively s...

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Chemical Research in Chinese Universities 2012年第6期28卷 1017-1021页

作者： SUN Ran CHANG Li-ping WANG Kai-chen SUN Hong-yan CONG Xian-ling Tissue bank China-Japan Union Hospital Jilin University Changchun 130033 P. R. China Department of Cardiopathy the Affiliated Hospital of Changchun University of Chinese Medicine Changchun 130021 P. R. China Department of Urinary Surgery Department of Dermatology China-Japan Union Hospital Jilin University Changchun 130033 P. R. China

Studies have shown that aberrant DNA methylation of apoptotic protease activating factor-1（APAF1） is an important epigenetic mechanism of gene regulation in the progression of bladder *** this article,we have proved that procaine,an inhibitor of DNA methyltransferases,could inhibit the proliferation of T24 and 5637 human bladder cancer cells by inducing their *** mechanism studies reveal that procaine could induce demethylation of APAF1 gene in T24 or 5637 cells,subsequently activating caspase-3/*** was also shown that the serum soluble fas ligand（sFasL） was activated,and the expression of matrix metallopeptidase 9（MMP-9） was *** seems to induce cell death by different pathways,and it might be used as a potential agent for bladder cancer treatment.

关键词： Bladder cancer cell Epigenetics DNA methylation Apoptotic protease activating factor-1(APAF1) Procaine

Sperm DNA Fragmentation may Influence IUI Outcome but could be Treated by ICSI: Evidence from Human Sperm bank

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Journal of Reproduction and Contraception 2014年第3期25卷 165-176页

作者： Hui LU Yong LIU Zi-jue ZHU Xiao-rong CAO Yu-fang XIAO Yong ZHU Wen-bo SHI Can SUN Feng YAN Zheng LI Department of Urology Renji Hospital School of Medicine Shanghai Jiao Tong University Shanghai Human Sperm Bank Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics

Objective To determine the correlation between semen parameters, sperm DNA damage, progressive motility （PR）, morphology and intrauterine insemination （IUI）/ intracyto- plasmic sperm injection （ICSI） outcomes. Methods all the donors providing the samples in this study were recruited by Shang- hai Human Sperm bank. For IUI, 122 donors were divided into group A （n=60） and group B （n=62）. Group A had a higher pregnancy rate while group B had a lower pregnancy rate （3.86 ± 1.50% vs 0.18 ± 0.52%）. For ICSI, 45 donors were divided into group C with a higher pregnancy rate （77. 78 ± 17.21%, n=23）, group D with a lower pregnancy rate （40. 73 ± 19.19%, n=22） and group E with an average preg- nancy rate in the sperm bank （48.96 ± 12.08%, n=23）. Semen analysis, morphology and DNA damage were assessed on samples retained in the sperm bank. Fresh semen samples were also collected and corresponding semen analyses data was included along with the pregnancy rates. Results No significant difference was found in the population characteristics between groups A and B, while there was a significant difference in sperm DNA fragmetation index （DFI） and morphology between the two groups （P〈0.05）, but not in other semen parameters （P〉0.05）. There was no significant difference in population characteristics between groups C, D and E while the DFI of group D was significant higher than groups C and E （P〈0.01）, but there was no significant difference in other semen parameters （P〉0.05）.Conclusion DFI might be a good predictor for IUI outcomes. Infertile couples with a high DFI should choose ICSI treatment instead of IUI. DFI should be a routine screening marker used to screen for sperm donors.

关键词： DNA fragmentation intrauterine insemination (IUI) intracytoplasmic sperminjection (ICSI) semen analysis outcome

Evaluation of inactivated COVID-19 vaccine on semen parameters in reproductive-age males:a retrospective cohort study

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Asian Journal of Andrology 2022年第5期24卷 441-444页

作者： Hong Zhu Xiao Wang Feng Zhang Yong Zhu Mei-Rong Du Zhi-Wen Tao Can Sun Hai-Tao Ma Ya-Dong Li Guo-Qing Liang Jie Ren Bai-Lan Feng Feng Jiang Human Sperm bank Obstetrics and Gynecology HospitalFudan UniversityShanghai 200011China Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases Obstetrics and Gynecology HospitalFudan UniversityShanghai 200011China Department of Andrology Shanghai Ji Ai Genetics and IVF institute China-USA CenterShanghai 200011China

During the coronavirus disease(COVID-19)epidemic,there have been concerns about the impact of vaccines on people’s fertility,including the fertility of those who are currently preparing for pregnancy and those who might become pregnant in ***,there is still a lack of research on the effect of the COVID-19 vaccine on male fertility,and it is not surprising that couples and donors have concerns regarding *** this study,a retrospective cohort study was conducted to examine semen quality before and after receipt of the inactivated COVID-19 *** were no statistically significant changes in semen parameters(volume,sperm concentration,progressive motility,and total progressive motile count)after two doses of vaccine(all P>0.05).In summary,our study updates the most recent studies on the effects of the COVID-19 vaccine on male fertility,and the information from this study could be used to guide fertility recommendations for assisted reproductive technology(ART)patients and donors.

关键词： assisted reproductive technology coronavirus disease vaccine male fertility semen parameters sperm donation

MKK7-mediated phosphorylation of JNKs regulates the proliferation and apoptosis of human spermatogonial stem cells

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World Journal of Stem Cells 2021年第11期13卷 1797-1812页

作者： Zeng-Hui Huang Chuan Huang Xi-Ren Ji Wen-Jun Zhou Xue-Feng Luo Qian Liu Yu-Lin Tang Fei Gong Wen-Bing Zhu Institute of Reproductive and Stem Cell Engineering Central South UniversityChangsha 410008Hunan ProvinceChina Department of Reproductive Center Reproductive and Genetic Hospital of CITIC-XiangyaChangsha 410008Hunan ProvinceChina Department of Sperm bank Reproductive and Genetic Hospital of CITICXiangyaChangsha 410008Hunan ProvinceChina

BACKGROUND Human spermatogonial stem cells(SSCs)are the basis of ***,little is known about the developmental regulatory mechanisms of SSC due to sample origin and species *** To investigates the mechanisms involved in the proliferation of human *** The expression of mitogen-activated protein kinase kinase 7(MKK7)in human testis was identified using immunohistochemistry and western blotting(WB).MKK7 was knocked down using small interfering RNA,and cell proliferation and apoptosis were detected by WB,EdU,cell counting kit-8 and fluorescenceactivated cell *** bioinformatic analysis,the interaction of MKK7 with c-Jun N-terminal kinases(JNKs)was verified by protein co-immunoprecipitation and *** phosphorylation of JNKs was inhibited by SP600125,and the phenotypic changes were detected by WB,cell counting kit-8 and fluorescenceactivated cell *** MKK7 is mainly expressed in human SSCs,and MKK7 knockdown inhibits SSC proliferation and promotes their ***7 mediated the phosphorylation of JNKs,and after inhibiting the phosphorylation of JNKs,the phenotypic changes of the cells were similar to those after MKK7 *** expression of MKK7 was significantly downregulated in patients with abnormal spermatogenesis,suggesting that abnormal MKK7 may be associated with spermatogenesis *** MKK7 regulates the proliferation and apoptosis of human SSC by mediating the phosphorylation of JNKs.

关键词： MKK7 Spermatogonial stem cell Proliferation Apoptosis JNKs

Comparison of intracytoplasmic sperm injection(ICSI)outcomes in infertile men with spermatogenic impairment of differing severity

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Asian Journal of Andrology 2022年第3期24卷 299-304页

作者： Ping Ping Zhong Zheng Yi Ma Sha-Sha Zou Xiang-Feng Chen Center for Reproductive Medicine Ren Ji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200135China Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics Shanghai 200135China Shanghai Human Sperm bank Shanghai 200135China

The extent of spermatogenic impairment on intracytoplasmic sperm injection(ICSI)outcomes and the risk of major birth defects have been little *** this study,we evaluated the relationship between various spermatogenic conditions,sperm origin on ICSI outcomes,and major birth defects.A total of 934 infertile men attending the Center for Reproductive Medicine of Ren Ji hospital(Shanghai,China)were classified into six groups:nonobstructive azoospermia(NOA;n=84),extremely severe oligozoospermia(esOZ;n=163),severe oligozoospermia(sOZ,n=174),mild oligozoospermia(mOZ;n=148),obstructive azoospermia(OAZ;n=155),and normozoospermia(NZ;n=210).Rates of fertilization,embryo cleavage,high-quality embryos,implantation,biochemical and clinical pregnancies,abortion,delivery,newborns,as well as major birth malformations,and other newborn outcomes were analyzed and compared among *** NOA group showed a statistically lower fertilization rate(68.2%vs esOZ 77.3%,sOZ 78.0%,mOZ 73.8%,OAZ 76.6%,and NZ 79.3%,all Pall Pally significant differences in rates of embryo cleavage,high-quality embryos,biochemical and clinical pregnancies,abortions,deliveries,major birth malformations,and other newborn outcomes in the six *** results showed that NOA only negatively affects some embryological outcomes such as fertilization *** was no evidence of differences in other embryological and clinical outcomes with respect to sperm source or spermatogenic *** failure and sperm origins do not impinge on the clinical outcomes in ICSI treatment.

关键词： intracytoplasmic sperm injection nonobstructive azoospermia normozoospermia obstructive azoospermia oligozoospermia

Omission of breakfast and risk of gastric cancer in Mexico

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World Journal of Gastrointestinal Oncology 2012年第11期4卷 223-229页

作者： Monserrat Verdalet-Olmedo Clara Luz Sampieri Jaime Morales-Romero Hilda Montero-L de Guevara lvaro Manuel Machorro-Castao Kenneth León-Córdoba Institute of Public Health University of Veracruz Xalapa Veracruz CP 91190 Mexico Blood bank Dr. Miguel Dorantes Hospital Xalapa Veracruz CP 91120 Mexico Department of Gastroenterology Dr. Miguel Dorantes Mesa Hospital Xalapa Veracruz CP 91120 Mexico

AIM: To investigate factors associated with gastric cancer (GC) in the Mexican population using a validated questionnaire. METHODS: We designed and validated in Spanish a Questionnaire to Find Factors Associated with Diseases of the Digestive Tract using GC as a model. A cross-sectional study using 49 subjects, with confirmed histopathological GC diagnosis, and 162 individuals without GC participated. Odds ratio and 95% CIs were estimated in univariate and multivariate analysis adjusted for possible confounding factors. In order to match age groups, a multivariate sub-analysis was performed in subjects ≥ 39 years of age and in females and males separately. RESULTS: In the univariate analysis, we found an association between GC and education to primary level or below, low socioeconomic status, the use of dental prostheses, omission of breakfast, consumption of very hot food and drink, addition of salt to prepared foods, consumption of salt-preserved foods and the pattern of alcohol consumption. We found protection against GC associated with the use of mouthwash, food refrigeration and regular consumption of fruit and vegetables. In the multivariate sub-analysis with subjects of ≥ 39 years, the omission of breakfast was identified as a risk factor for GC. CONCLUSION: Our study suggests an association between the omission of breakfast and the failure to refrigerate food with GC in the Mexican population.

关键词： Gastric cancer Questionnaire Risk factors Omission of breakfast

ASSOCIATION OF HLA WITH GRAVES' DISEASE IN CHINESE

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中华医学杂志(英文版) 1987年第4期100卷 296-298页

作者：童钟杭朱艮严力行朱俊丰以贵徐根 Research Institute of Endocrinology Blood bank First Affiliated hospital Zhejiang Medical University Hangzhou

HLA-A, B typing was studied on 100 unrelatedGraves’ disease patients of Han Nationality in Hang-zhou. 100 healthy donors served as controls. A marked increase in the frequency of HLA-Aantigen was observed in patients with Graves’ diseasewhich was consistant and significantly differentfrom the normal, even after correction. This showeda close association between HLA-Aand Graves’disease. The frequency of Bantigen tended todecrease in patients with Graves’ disease as com-pared with the controls after correction. A negativecorrelation was found between HLA-Band Graves’disease.

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Sperm donation and its application in,China： a 7-year multicenter retrospective study

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Asian Journal of Andrology 2011年第4期13卷 644-648页

作者： Ping Ping Wen-Bing Zhu Xin-Zong Zhang Yu-Shan Li Quan-Xian Wang Xiao-Rong Cao Yong Liu Hui-Li Dai Yi-Ran Huang Zheng Li Department of Urology Renji Hospital Shanghai Jiao Tong University School of Medicine Shanghai Human Sperm Bank Shanghai 200001 China Institute of Human Reproduction and Stem Cell Engineering Human Sperm Bank Central South University Changsha 410078 China Zhejiang Institute of Planned Parenthood Research and Zhejiang Human Sperm bank Hangzhou 310012 China Human Sperm bank The Third Affiliated Hospital of Zhengzhou University Zhengzhou 450052 China

Sperm donation in China is different from that in other countries due to cultural, social and political factors. This research presents the current status of sperm donation in China's Mainland and highlights some problems. Between January 2003 and December 2009, 19 471 sperm donors were screened totally and 6467 donors （33.2%） were recruited. The primary reasons for non-recruitment were either inadequate semen parameters （55.0%） or positive results for sexually transmitted diseases （7.9%）. There were 327 （1.7%） qualified donors who withdrew from the program because of frustration related to failed semen parameters, participation merely for free medical tests or job transfer. A questionnaire investigating donor intention, as well as other concerns associated with sperm donation, was distributed to 516 potential donors. all potential donors indicated their primary motivation as altruism, while 90.9% mentioned monetary reward as a second motivating factor. Approximately 93.4% of donors expressed some apprehension about the risk of consanguineous mating and the protection of their identity. Over the past 7 years, 488 389 vials of donors＇ semen have been cryopreserved. In 36 438 artificial insemination with donor sperm （AID） cycles, the clinical pregnancy rate was 23.9% and the live birth rate was 16.6%, In 7148 in vitro fertilization cycles, the clinical pregnancy rate was 45.8% and the live birth rate was 35.2%. Human sperm banks have been strictly monitored to ensure that each sperm donor can only impregnate five women nationwide. There is still a large gap between the supply and demand for sperm donation which may be solved by updated guidelines.

关键词： artificial insemination with donor sperm donor in vitro fertilization sperm donation