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Molecular characterization and functional analysis of two new lysozyme genes from soybean cyst nematode(Heterodera glycines)

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Journal of Integrative Agriculture 2019年第12期18卷 2806-2813页

作者： WANG Ning PENG Huan LIU Shi-ming HUANG Wen-kun Ricardo Holgado Jihong Liu-Clarke PENG De-liang state Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193P.R.China NIBIO Norwegian Institute of Bioeconomy ResearchPb 115N0-1431 AsNorway

Soybean cyst nematode(SCN, Heterodera glycines(I.)) is one of the most important soil-borne pathogens for soybeans. In plant parasitic nematodes, including SCN, lysozyme plays important roles in the innate defense system. In this study, two new lysozyme genes(Hg-lys1 and Hg-lys2) from SCN were cloned and characterized. The in situ hybridization analyses indicated that the transcripts of both Hg-lys1 and Hg-lys2 accumulated in the intestine of SCN. The q RT-PCR analyses showed that both Hg-lys1 and Hg-lys2 were upregulated after SCN second stage juveniles(J2 s) were exposed to the Grampositive bacteria Bacillus thuringiensis, Bacillus subtilis or Staphylococcus aureus. Knockdown of the identified lysozyme genes by in vitro RNA interference caused a significant decrease in the survival rate of SCN. All of the obtained results indicate that lysozyme is very important in the defense system and survival of SCN.

关键词： soybean cyst nematode lysozyme Hg-lys1 Hg-lys2 innate defense against bacteria

A Simple CRISPR/Cas9 System for Multiplex Genome Editing in Rice

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Journal of Genetics and Genomics 2015年第12期42卷 703-706页

作者： Chun Wang Lan Shen Yaping Fu Changjie Yan Kejian Wang state Key Laboratory of Rice Biology China National Rice Research Institute Chinese Academy of Agricultural Sciences Key Laboratory of Plant Functional Genomics Ministry of EducationYangzhou University

Generating mutants bearing multiple gene modifications is essential for determining the functions of gene family members with redundant functions, or for analyzing epistatic re- lationships in genetic pathways. Using conventional methods, mutants with multiple gene mutations are generated by several rounds of intercrossing plants carrying a single mutation and identification of the offspring. This process is both timeconsuming and labor-intensive. Moreover, if the genes of interest are closely linked, multiple mutations can not be generated （Wijnker and de Jong, 2008）.

关键词： A Simple CRISPR/Cas9 System for Multiplex Genome Editing in Rice PDS Kpn RNA gene PCR

Controlled fabrication of Fe_(3)N@NG composites as superior oxygen evolution reaction electrocatalysts

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Carbon Resources Conversion 2024年第2期7卷 13-16页

作者： Yang Song Tongya Tian Changming Li Xi Zeng Sen Zhang Li Chen Zhenghua Yang Qizhe Ji Xianglong Zhao Feiyong Chen Resources and Environment Innovation institute Shandong Jianzhu UniversityJinan 250101PR China School of Science Shandong Jianzhu UniversityJinan 250101PR China Huzhou Nanxun District Jianda Ecological Environment Innovation Center Shandong Jianzhu UniversityHuzhouZhejiang 313009PR China state Environmental Protection Key Laboratory of Food Chain Pollution Control Beijing Technology and Business UniversityBeijing 100048PR China

We report the controlled fabrication of nitrogen doped graphene(NG)nanoplates,which are uniformly decorated with iron nitride(Fe_(3)N)nanoparticles,via ball milling of mixtures of graphite and iron nitrates and the following ammonia *** obtained Fe_(3)N@NG composites demonstrate excellent electrocatalytic activity and durability for oxygen evolution reaction,both of which outperform those of the state-of-the-art iridium oxide *** may be attributed to nitrogen doping as well as the synergistic effect between Fe3N and graphene nanoplates.

关键词： Graphene Iron nitrides Ball milling Oxygen evolution reaction Electrocatalysts

Reanalysis on the Spectral Characteristics and Materials of Jade Jue Unearthed from Lijiashan Ancient Tomb in Yunnan Province, China

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宝石和宝石学杂志（中英文） 2024年第2期26卷 48-55页

作者： Ting Fang Jifu Liu Mingxing Yang Gemmological institute China University of GeosciencesWuhan 430074China Hubei Key research Base of Humanities and Social Sciences--Center for Jewelry Heritage and Innovation Wuhan 430074China

Jade ware Jue is one of the most fashionable ornament in ancient China from the Neolithic Age to the Han *** this study,the remnants of jade ware Jue,from Lijiashan ancient tomb group have been tested and analyzed *** samples through a light etch surface showing glass luster and those with a severe etch surface showing earthen *** the aid of X-ray fluorescence Spectrometer(X-ray Fluorescence)test and X-ray diffraction(XRD)detection,the results show that tremolite is not the main mineral of the *** main components of the tested samples with light erosion are antigorite and talc,although the main components of the samples with severe erosion are calcite containing calcium carbonate ***,the research on jade ware Jue in Yunnan culture has not only contributed to our further understanding of the jade objects from Yunnan culture and the variety of jade material located in the Yunnan *** also provides a basis for the trace of the history and culture through the distribution and evolution of burial hierarchy *** the same time,the microscopic observation of the tested samples manifested a residual trace of“string”at the perforation,which has played a critical role in restoring the wearing of jade ware Jue from the tombs of the ancient Yunnan state and the handicraft skills at that time.

关键词： Dian culture jade Jue corroded ancient jade XRD erosion mechanism Yunnan,China

Phenylalanine deprivation inhibits multiple myeloma progression by perturbing endoplasmic reticulum homeostasis

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Acta Pharmaceutica Sinica B 2024年第8期14卷 3493-3512页

作者： Longhao Cheng Xiaoxue Wang Aijun Liu Ying Zhu Hu Cheng Jiangling Yu Lili Gong Honglin Liu Guolin Shen Lihong Liu institute of Clinical Medical Sciences ChinaeJapan Friendship HospitalCapital Medical UniversityBeijing 100029China Department of Pharmacy ChinaeJapan Friendship HospitalBeijing 100029China Beijing Chao-Yang Hospital Capital Medical UniversityBeijing 100020China institute of Chemicals Safety Chinese Academy of Inspection and QuarantineBeijing 100020China

Amino acid metabolic remodeling is a hallmark of cancer,driving an increased nutritional demand for amino *** acids are pivotal for energetic regulation,biosynthetic support,and homeostatic maintenance to stimulate cancer ***,the role of phenylalanine in multiple myeloma(MM)remains ***,we demonstrate that phenylalanine levels in MM patients are decreased in plasma but elevated in bone marrow(BM)*** the treatment,phenylalanine levels increase in plasma and decrease in *** suggests that changes in phenylalanine have diagnostic value and that phenylalanine in the BM microenvironment is an essential source of nutrients for MM *** requirement for phenylalanine by MM cells exhibits a similar *** phenylalanine utilization suppresses MM cell growth and provides a synergistic effect with Bortezomib(BTZ)treatment in vitro and murine ***,phenylalanine deprivation induces excessive endoplasmic reticulum stress and leads to MM cell apoptosis through the ATF3eCHOPeDR5 *** with ATF3 significantly affects phenylalanine deprivation *** conclusion,we have identified phenylalanine metabolism as a characteristic feature of MM metabolic *** is necessary for MM proliferation,and its aberrant demand highlights the importance of lowphenylalanine diets as an adjuvant treatment for MM.

关键词： Multiple myeloma Phenylalanine Amino acid metabolism Nutrient deprivation Cancer therapy Endoplasmic reticulum stress ATF3 Apoptosis

Effects of GS3 and GL3.1 for Grain Size Editing by CRISPR/Cas9 in Rice

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Rice science 2020年第5期27卷 405-413,I0024页

作者： Chen Yuyu Zhu Aike Xue Pao Wen Xiaoxia Cao Yongrun Wang Beifang Zhang Yue Shah Liaqat Cheng Shihua Cao Liyong Zhang Yingxin state Key Laboratory of Rice Biology and Chinese National Center for Rice Improvement China National Rice Research InstituteHangzhou 311401China National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural UniversityWuhan 430070China Nanch ong Academy of agricultural Sciences Ncmchong 637000China

Grain size is one of key agronomic traits associated with grain yield and grain quality. Both major quantitative trait loci GS3 and GL3.1 play a predominant role in negative regulation of grain size. In this study, a CRISPR/Cas9-mediated multiplex genome editing system was used to simultaneously edit GS3 and GL3.1 in a typical japonica rice Nipponbare. In T1 generation, we found that gs3 formed slender grain with lower chalkiness percentage, while gs3gl3.1 produced larger grain with higher chalkiness percentage. In terms of other agronomic traits, flag leaf size, grain number and grain yield of both gs3 and gs3gl3.1 mutants were affected. It is noteworthy that gs3 and gs3gl3.1 mutants both led to dramatical reduction of grain number, thereby decreased grain yield. In conclusion, these results indicated that knockout of GS3 and GL3.1 could rapidly improve grain size, but probably have some negative influences on grain quality and grain yield.

关键词： rice GS3 GL3.1 grain size grain quality grain yield CRISPR/Cas9

Effects of different forms of yeast Saccharomyces cerevisiae on growth performance,intestinal development,and systemic immunity in early-weaned piglets

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Journal of Animal Science and Biotechnology 2016年第1期7卷 78-85页

作者： Zongyong Jiang Shaoyong Wei Zhilin Wang Cui Zhu Shenglan Hu Chuntian Zheng Zhuang Chen Youjun Hu Li Wang Xianyong Ma Xuefen Yang Key Laboratory of Animal Nutrition and Feed(South China) Ministry of Agriculture of ChinaState Key Laboratory of Livestock and Poultry BreedingGuangdong Public Laboratory of Animal Breeding and NutritionInstitute of Animal ScienceGuangdong Academy of Agricultural Sciences Agro-biological Gene research Center Guangdong Academy of Agricultural Sciences

The present study was conducted to determine effects of different forms of yeast （Saccharomyces cerevisiae, strain Y200007） on the growth performance, intestinal development, and systemic immunity in early-weaned piglets. A total of 96 piglets （14-d old, initial average body weight of 4.5 kg） were assigned to 4 dietary treatments：（1） basa diet without yeast （Control）; （2） basal diet supplemented with 3.00 g/kg live yeast （LY）; （3） basal diet supplemented with 2.66 g/kg heat-killed whole yeast （HKY）; and （4） basal diet supplemented with 3.00 g/kg superfine yeast powders （SFY）. Diets and water were provided ad libitum to the piglets during 3-week experiment. Growth performance of piglets was measured weekly. Samples of blood and small intestine were collected at days 7 and 21 of experiment. Dietary supplementation with LY and SFY improved G：F of piglets at days ]-21 of the experiment （P 〈 0.05） compared to Control group. Serum concentrations of growth hormone （GH）, triiodothyronine （T3）, tetraiodothyronine （T4）, and insulin growth factor 1 （iGF-1） in piglets at day 21 of the experiment were higher when fed diets supplemented with LY and SFY than those in Control group （P 〈 0.05）. Compared to Control group, contents of serum urea nitrogen of piglets were reduced by the 3 yeast-supplemented diets （P 〈 0.05）. Diets supplemented with LY increased villus height and villus-to-crypt ratio in duodenum and jejunum of piglets （P 〈 0.05） compared to other two groups at day 7 of the experiment. Feeding diets supplemented with LY and SFY increased （P 〈 0.05） serum concentrations of IgA, IL-2, and IL-6 levels in piglets compared to Control. The CD4＋/CD8＋ ratio and proliferation of T-lymphocytes in piglets fed diets supplemented with LY were increased compared to that of Control group at day 7 of the experiment （P 〈 0.05）. In conclusion, dietary supplementation with both LY and SFY enhanced feed conversion, small intestinal development, and systemic immunity in early-weaned piglets

关键词： Growth performance Immunity Intestinal development Piglets Saccharomyces cerevisiae Yeast

A novel CCCH-type zinc finger protein SAW1 activates OsGA20ox3 to regulate gibberellin homeostasis and anther development in rice

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Journal of Integrative Plant Biology 2020年第10期62卷 1594-1606页

作者： Bin Wang Ruiqiu Fang Faming Chen Jingluan Han Yao-Guang Liu Letian Chen Qinlong Zhu state Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources South China Agricultural UniversityGuangzhou 510642China Guangdong Laboratory for Lingnan Modern Agriculture Guangzhou 510642China Guangdong Provincial Key Laboratory of Protein Function and Regulation in agricultural Organisms South China Agricultural UniversityGuangzhou 510642China College of Life Sciences South China Agricultural UniversityGuangzhou 510642China Dongyang institute of Maize research Zhejiang Academy of Agricultural SciencesDongyang 322100China

Male sterility is a prerequisite for hybrid seed *** phytohormone gibberellin(GA)is in-volved in regulating male reproductive development,but the mechanism underlying GA homeostasis in anther development remains less ***,we report the isolation and characterization of a new positive regulator of GA homeostasis,swollen anther wall 1(SAW1),for anther development in rice(Oryza sativa L.).Rice plants carrying the recessive mutant allele saw1 produces abnormal anthers with swollen anther wall and aborted *** regularly interspaced short palindromic repeats(CRISPR)/CRIPSR-associated protein 9-mediated knockout of SAW1 in rice generated similar male sterile ***1 encodes a novel nucleus-localizing CCCH-tandem zinc finger protein,and this protein could directly bind to the promoter region of the GA synthesis gene OsGA20ox3 to induce its anther-specific *** the saw1 anther,the significantly decreased OsGA20ox3 expression resulted in lower bio-active GA content,which in turn caused the lower expression of the GA-inducible anther-regulator gene ***,our results disclose the mechanism of the SAW1-GA20ox3-GAMYB pathway in controlling rice anther development,and provide a new target gene for the rapid generation of male sterile lines by genome editing for hybrid breeding.

关键词： sterile homeostasis anther

Cloning and Expression of Calcium-Dependent Protein Kinase (CDPK) Gene Family in Common Tobacco (Nicotiana tabacum)

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agricultural Sciences in China 2009年第12期8卷 1448-1457页

作者： TAI Shuai-shuai LIU Guan-shan SUN Yu-he CHEN Jia Tobacco research institute Chinese Academy of Agricultural Sciences/Key Laboratory of Tobacco Quality Control Ministry of Agriculture Qingdao 266101 P.R. China state Key Laboratory of Plant Physiology and Biochemistry/College of Biological Sciences China Agricultural University Beijing 100193 P.R.China

To further study the function of calcium-dependent protein kinase （CDPK） gene family in common tobacco （Nicotiana tabacum）, it is necessary to isolate more CDPKs from common tobacco and describe the sequence characteristics, evolutionary relationship and gene expression. Reverse transcription-PCR （RT-PCR）, rapid amplification of cDNA （RACE） and bioinformatics methods were used to isolate CDPKs from common tobacco. A phylogenetic tree was created using the MEGA4.0 program and expression patterns of the three full-length CDPK genes were studied by RT-PCR. After all aforementioned efforts, we obtained eight additional common tobacco CDPK genes, of which three possessed complete open reading frames （ORFs）. Phylogenetic analysis divided 1 1 full-length Nicotiana CDPK genes into four subfamilies, and two putative common tobacco and Arabidopsis orthologous CDPK genes might correspond to well-conserved functions. Three full-length CDPK genes in common tobacco were detected in all tobacco organs tested, but their expression patterns were significantly different. Eight non-redundant common tobacco CDPK genes were isolated in this study. Along with the previously characterized CDPK genes, at least 15 members of the CDPK family exist in common tobacco. This work establishes a foundation for a genome-wide study of this important gene family in common tobacco.

关键词： common tobacco (Nicotiana tabacum) CDPK phylogenetic tree gene expression