Molecular characterization and functional analysis of two new lysozyme genes from soybean cyst nematode(Heterodera glycines)

作     者：WANG Ning PENG Huan LIU Shi-ming HUANG Wen-kun Ricardo Holgado Jihong Liu-Clarke PENG De-liang 

作者机构：State Key Laboratory for Biology of Plant Diseases and Insect PestsInstitute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193P.R.China NIBIONorwegian Institute of Bioeconomy ResearchPb 115N0-1431 AsNorway 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2019年第18卷第12期

页      面：2806-2813页

核心收录：

学科分类：0710[理学-生物学] 0832[工学-食品科学与工程（可授工学、农学学位）] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1002[医学-临床医学] 0905[农学-畜牧学] 0906[农学-兽医学] 0901[农学-作物学] 0703[理学-化学] 100214[医学-肿瘤学] 0902[农学-园艺学] 0713[理学-生态学] 10[医学] 

基　　金：supported the Central Public-Interest Scientific Institution Basal Research Fund, China (Y2019GH03) the Special Fund for Agro-Scientific Research in the Public Interest of China (210503114) SINOGRAIN Ⅱ (CHN-17/0019): Technological Innovation to Support Environmentally-Friendly Food Production and Food Safety Under a Changing Climate-Opportunities and Challenges for Norway-China Cooperation 

主　　题：soybean cyst nematode lysozyme Hg-lys1 Hg-lys2 innate defense against bacteria 

摘      要：Soybean cyst nematode(SCN, Heterodera glycines(I.)) is one of the most important soil-borne pathogens for soybeans. In plant parasitic nematodes, including SCN, lysozyme plays important roles in the innate defense system. In this study, two new lysozyme genes(Hg-lys1 and Hg-lys2) from SCN were cloned and characterized. The in situ hybridization analyses indicated that the transcripts of both Hg-lys1 and Hg-lys2 accumulated in the intestine of SCN. The q RT-PCR analyses showed that both Hg-lys1 and Hg-lys2 were upregulated after SCN second stage juveniles(J2 s) were exposed to the Grampositive bacteria Bacillus thuringiensis, Bacillus subtilis or Staphylococcus aureus. Knockdown of the identified lysozyme genes by in vitro RNA interference caused a significant decrease in the survival rate of SCN. All of the obtained results indicate that lysozyme is very important in the defense system and survival of SCN.