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Journal of environmental Sciences 2012年第5期24卷 926-933页

作者： Peter Christie agri-environment branch Agri-Food and Biosciences Institute Newforge Lane Belfast BT9 5PX United Kingdom

The contamination of soils by polycyclic aromatic hydrocarbons （PAHs） is a widespread environmental problem and the remediation of PAHs from these areas has been a major concern. The effectiveness of many in situ bioremediation systems may be constrained by low contaminant bioavailability due to limited aqueous solubility or a large magnitude of sorption. The objective of this research was to evaluate the effect of methyl-β-cyclodextrin （MCD） on bioaugmentation by Paracoccus sp. strain HPD-2 of an aged PAH-contaminated soil. When 10% （W/W） MCD amendment was combined with bioaugmentation by the PAH-degrading bacterium Paracoccus sp. strain HPD-2, the percentage degradation of total PAHs was significantly enhanced up to 34.8%. Higher counts of culturable PAH- degrading bacteria and higher soil dehydrogenase and soil polyphenol oxidase activities were observed in 10% （W/W） MCD-assisted bioaugmentation soil. This MCD-assisted bioaugmentation strategy showed significant increases （p 〈 0.05） in the average well color development （AWCD） obtained by the BIOLOG Eco plate assay, Shannon-Weaver index （H） and Simpson index （λ） compared with the controls, implying that this strategy at least partially restored the microbiological functioning of the PAH-contaminated soil. The results suggest that MCD-aided bioaugmentation by Paracoccus sp. strain HPD-2 may be a promising practical bioremediation strategy for aged PAH-contaminated soils.

关键词： polycyclic aromatic hydrocarbons methyl-β-cyclodextrin biodegradation Paracoccus sp. strain HPD-2 microbial activity

New glucocerebrosidase antibodies can advance research in the field of neurodegenerative disorders

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Neural Regeneration Research 2025年第4期20卷 1085-1086页

作者： Charis Ma Krystyna Rytel Yu Chen Ellen Sidransky Medical Genetics branch National Human Genome Research InstituteNational Institutes of HealthBethesdaMDUSA

In medical research,there are times when the introduction of a new tool can launch scientific discovery in new *** antibody development may be considered mundane,in the field of glucocerebrosidase(GCase)research,the dearth of validated antibodies for different applications has impeded progress in studies of disease pathogenesis and therapeutic *** recent introduction of new,rigorously evaluated antibodies can now propel research into the link between glucocerebrosidase and Parkinson’s disease(PD)as well as aspects of the pathobiology of Gaucher disease(Jong et al.,2024).

关键词： antibodies cerebro

Dissipation of polycyclic aromatic hydrocarbons and microbial activity in a field soil planted with perennial ryegrass

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Frontiers of environmental Science & Engineering 2012年第3期6卷 330-335页

作者： Dengqiang FU Ying TENG Yuanyuan SHEN Mingming SUN Chen TU Yongming LUO Zhengao LI Peter CHRISTIE Key Laboratory of Soil environment and Pollution Remediation Institute of Soil ScienceChinese Academy of SciencesNanjing 210008China agri-environment branch Agri-Food and Biosciences InstituteNewforge LaneBelfast BT95PXUK

Dissipation and plant uptake of polycyclic aromatic hydrocarbons （PAHs） in contaminated agricul- tural soil planted with perennial ryegrass were investigated in a field experiment. After two seasons of grass cultivation the mean concentration of 12 PAHs in soil decreased by 23.4% compared with the initial soil. The 3-, 4-, 5-, and 6- ring PAHs were dissipated by 30.9%, 25.5%, 21.2%, and 16.3% from the soil, respectively. Ryegrass shoots accumulated about 280 ***1, shoot dry matter biomass reached 2.48 x 104kg-ha1, and plant uptake accounted for about 0.99% of the decrease in PAHs in the soil. Significantly higher soil enzyme activities and microbial community functional diversity were observed in planted soil than that in the unplanted control. The results suggest that planting ryegrass may promote the dissipation of PAHs in long-term contaminated agricultural soil, and plant-promoted microbial degradation may be a main mechanism of phytoremediation.

关键词： perennial ryegrass polycyclic aromatic hydrocarbon bioremediation plant uptake soil microbial activity

Estimating total nitrogen deposition in agroecosystems in northern China during the wheat cropping season

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Journal of Arid Land 2010年第1期2卷 2-8页

作者： Christie PETER Fangmeier ANDREAS College of Resources and environmental Sciences China Agricultural University agri-environment branch Agri-Food and Biosciences Institute Institute of Plant Ecology and Landscape University of Hohenheim

Atmospheric nitrogen (N) deposition has been poorly documented in northern China, an intensive agricultural and industrial region with large emissions of NHx and NOy. To quantify N deposition, total airborne N deposition was determined at three agricultural sites using a manual integrated total nitrogen input (ITNI) system during growth of winter wheat (Triticum aestivum L.) and Italian ryegrass (Lolium multiflorum Lam.) from September 2005 to May 2006. Total estimated N deposition averaged 54.9 and 43.2 kg N/hm2 across the three sites when wheat was grown to flowering and maturing, respectively. The average value was 50.2 kg N/hm2 when ryegrass was the indicator plant. Both indicator species gave similar total airborne N input results. The intermediate level of N supplied resulted in the highest N deposition, and the ratio of N acquired from deposition to total N content of the whole system decreased with increasing N supply to the roots. The contribution of atmospheric N to the total N content of the wheat and ryegrass sand culture systems ranged from 10% to 24%.

关键词： airborne nitrogen inputs 15N pool dilution method sand culture system biological indicators

Physiological and Antioxidant Responses of Germinating Mung Bean Seedlings to Phthalate Esters in Soil

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Pedosphere 2014年第1期24卷 107-115页

作者： MA Ting-Ting P.CHRISTIE LUO Yong-Ming TENG Ying Key Laboratory of Soil environment and Pollution Remediation Institute of Soil ScienceChinese Academy of Sciences University of Chinese Academy of Sciences agri-environment branch Agri-Food and Biosciences Institute Yantai Institute of Costal Zone Research Chinese Academy of Sciences

Single phytotoxicity of two representative phthalate esters(PAEs),di-n-butyl phthalate(DnBP)and bis(2-ethylhexyl)phthalate(DEHP),was tested in mung bean(Vigna radiata)seedlings germinated for 72 h in soils spiked with varying concentrations(0-500 mg kg^(-1)soil)of DnBP or *** added at up to 500 mg kg^(-1)soil exerted no significant effect on germination but both pollutants significantly inhibited root elongation(P<0.01),DEHP inhibited shoot elongation(P<0.01)and DnBP depressed biomass on a fresh weight basis(P<0.05).Seedling shoot and root malondialdehyde(MDA)contents tended to be stimulated by DnBP but inhibited by ***,increases in superoxide dismutase,peroxidase,ascorbate peroxidase and polyphenol oxidase activities,as well as glutathione(GSH)content,were induced at higher concentrations(e.g.,20 mg kg^(-1))of both *** of proline in both roots and shoots and the storage compounds,such as free amino acids and total soluble sugars,in whole plant was induced under the stress exerted by both *** general responses of mung bean seedlings indicated higher toxicity of DnBP than DEHP on primary growth,during which root elongation was a more responsive *** and GSH were more sensitive parameters in the roots than in the shoots and they might be recommended as physiologically sensitive parameters to assess the toxicity of PAE compounds in soils in future long-term studies.

关键词： glutathione malondialdehyde phytotoxicity root elongation storage compounds

Adsorption and desorption characteristics of diphenylarsenicals in two contrasting soils

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Journal of environmental Sciences 2013年第6期25卷 1172-1179页

作者： Anan Wang Shixin Li Ying Teng Wuxin Liu Longhua Wu Haibo Zhang Yujuan Huang Yongming Luo Peter Christie Key Laboratory of Soil environment and Pollution Remediation Institute of Soil ScienceChinese Academy of Sciences Graduate School of Chinese Academy of Sciences Yantai Institute of Coastal Zone Research Chinese Academy of Sciences agri-environment branch Agri-Food and Biosciences InstituteNewforge LaneBelfast BT9 5PXUK

Diphenylarsinic acid （DPAA） is formed during the leakage of aromatic arsenic chemical weapons in soils, is persistent in nature, and results in arsenic contamination in the field. The adsorption and desorption characteristics of DPAA were investigated in two typical Chinese soils, an Acrisol （a variable-charge soil） and a Phaeozem （a constant-charge soil）. Their thermodynamics and some of the factors influencing them （i.e., initial pH value, ionic strength and phosphate） were also evaluated using the batch method in order to understand the environmental fate of DPAA in soils. The results indicate that Acrisol had a stronger adsorption capacity for DPAA than Phaeozem. Soil DPAA adsorption was a spontaneous and endothermic process and the amount of DPAA adsorbed was affected significantly by variation in soil pH and phosphate. In contrast, soil organic matter and ionic strength had no significant effect on adsorption. This suggests that DPAA adsorption may be due to specific adsorption on soil mineral surfaces. Therefore, monitoring the fate of DPAA in soils is recommended in areas contaminated by leakage from chemical weapons.

关键词： diphenylarsinic acid adsorption and desorption chemical weapons residual soil contamination

Detection using mask adaptive transformers in unmanned aerial vehicle imagery

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Optoelectronics Letters 2025年第2期21卷 113-120页

作者： YE Huibiao FAN Weiming GUO Yuping WANG Xuna ZHOU Dalin China Telecommunication Corporation Zhejiang branch Innovation Center for Smart Medical Technologies & Devices Binjiang Institute of Zhejiang University School of Computing University of Portsmouth

Drone photography is an essential building block of intelligent transportation,enabling wide-ranging monitoring,precise positioning,and rapid ***,the high computational cost of transformer-based methods in object detection tasks hinders real-time result transmission in drone target detection ***,we propose mask adaptive transformer (MAT) tailored for such ***,we introduce a structure that supports collaborative token sparsification in support windows,enhancing fault tolerance and reducing computational *** structure comprises two modules:a binary mask strategy and adaptive window self-attention (A-WSA).The binary mask strategy focuses on significant objects in various complex *** A-WSA mechanism is employed to self-attend for balance perfomance and computational cost to select objects and isolate all contextual *** experiments on the challenging CarPK and VisDrone datasets demonstrate the effectiveness and superiority of the proposed ***,it achieves a mean average precision (mAP@0.5) improvement of 1.25%over car detector based on you only look once version 5 (CD-YOLOv5) on the CarPK dataset and a 3.75%average precision(AP@0.5) improvement over cascaded zoom-in detector (CZ Det) on the VisDrone dataset.

关键词： Drones

Proteomic response of wheat embryos to fosthiazate stress in a protected vegetable soil

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Journal of environmental Sciences 2012年第10期24卷 1843-1853页

作者： Chunyan Yin Ying Teng Yongming Luo Peter Christie Key Laboratory of Soil environment and Pollution Remediation Institute of Soil ScienceChinese Academy of SciencesNanjing 210008China Graduate University of Chinese Academy of Sciences Beijing 100049China Yantai Institute of Coastal Zone Research Chinese Academy of SciencesYantai 264003China agri-environment branch Agri-Food and Biosciences InstituteNewforge LaneBelfast BT95PXUnited Kingdom

A proteomic analysis of wheat defense response induced by the widely used organophosphorus nematicide fosthiazate is reported. Seed germination and two-dimensional gel electrophoresis （2-DE） experiments were performed using a Chinese wheat cultivar, Zhenmai No. 5. Root and shoot elongation decreased but thiobarbituric acid reactive substances （TBARS） content in embryos increased with increasing pesticide concentration. More than 1000 protein spots were reprodueibly detected in each silver-stained gel. Thirty-seven protein spots with at least 2-fold changes were identified using MALDI-TOF MS/MS analysis. Of these, 24 spots were up-regulated and 13 were down-regulated. Proteins identified included some well-known classical stress responsive proteins under abiotic or biotic stresses as well as some unusual responsive proteins. Ten responsive proteins were reported for the first time at the proteomic level, including fatty acyl CoA reductase, dihydrodipicolinate synthase, DEAD-box ATPase-RNA-helicase, fimbriata-like protein, waxy B 1, rust resistance kinase Lrl0, putative In2.1 protein, retinoblastoma-related protein 1, pollen allergen-like protein and S-adenosyl-L- methionine：phosphoethanolamine N-methyltransferase. The proteins identified were involved in several processes such as metabolism, defense/detoxification, cell structure/ceU growth, signal transduction]transcription, photosynthesis and energy. Seven candidate proteins were further analyzed at the mRNA level by RT-PCR to compare transcript and protein accumulation patterns, revealing that not all the genes were correlated well with the protein level. Identification of these responsive proteins may provide new insight into the molecular basis of the fosthiazate-stress response in the early developmental stages of plants and may be useful in stress monitoring or stress-tolerant crop breeding for environmentally friendly agricultural production.

关键词： fosthiazate proteomic wheat embryo stress response biological indicator

Natural ^(15)N Abundance in Winter Wheat Amended with Urea and Compost: A Long-Term Experiment

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Pedosphere 2013年第6期23卷 835-843页

作者： ZHOU Wei HU Chun-Sheng LI Ji P.CHRISTIE HE Xin-Hua JU Xiao-Tang Key Laboratory of agricultural Water Resources Center for Agricultural Resources ResearchInstitute of Genetics and Developmental BiologyChinese Academy of Sciences University of Chinese Academy of Sciences College of Resources and environmental Sciences China Agricultural University agri-environment branch Agri-Food and Biosciences Institute Northern Research Station USDA Forest Service

We investigated 15N abundance （δ15N） of winter wheat （Triticum aestivum cv. Jinmai 1） plants and soil at different growth stages in a field with a 13-year fertilization history of urea and compost, to determine whether or not the δ15N of plant parts can be used as an indicator of organic amendment with compost. Plant parts （roots, leaves, stems and grains） and soil were sampled at re-greening, jointing, grain filling and mature growth stages of winter wheat. There were significant differences between the urea and compost treatments in 815N of whole plants, plant parts and soil over the whole growing season. Determination of the δ15N of plant parts was more convenient than that of whole plant to distinguish between the application of organic amendment and synthetic N fertilizer.

关键词： detection growth stages natural δ15N organic food tissue

Phytotoxicity in seven higher plant species exposed to di-n-butyl phthalate or bis （2-ethylhexyl） phthalate

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Frontiers of environmental Science & Engineering 2015年第2期9卷 259-268页

作者： Tingting MA Ying TENG Peter CHRISTIE Yongming LUO Key Laboratory of Soil environment and Pollution Remediation of Chinese Academy of Sciences Institute of Soil Science Nanjing 210008 China Graduate University of Chinese Academy of Sciences Beijing 100049 China agri-environment branch Agri-Food and Biosciences Institute Newforge Lane Belfast BT9 5PX United Kingdom Yantai Institute of Costal Zone Research Chinese Academy of Sciences Yantai 264003 China

We investigated phytotoxicity in seven plant species exposed to a range of concentrations （0- 500 mg· kg^-1 soil） of di-n-butyl phthalate （DnBP） or his （2- ethylhexyl） phthalate （DEHP）, two representative phthalate esters （PAEs） nominated by USEPA as priority pollutants and known environmental estrogens. We studied seed germination, root elongation, seedling growth, biomass （fresh weight, FW） and malondialdehyde （MDA） content of shoots and roots of wheat （Triticum aestivum L.）, alfalfa （Medicago sativa L.）, perennial ryegrass （Lolium perenne）, radish （Raphanus sativus L.）, cucumber （Cucumis sativus L.）, oat （Avena sativa） and onion （Allium cepa L.）, together with monitoring of plant pigment content （chlorophyll a, b and earotinoids） in alfalfa, radish and onion shoots. Root elongation, seedling growth and biomass of the test species were generally inhibited by DnBP but not by DEHP, indicating a lower level of phytotoxicity of DEHP than of DnBP. MDA contents of four species were promoted by PAE exposure, but not in alfalfa, ryegrass or onion shoots, indicating lower sensitivity of these three species to PAE pollutants. Plant pigment contents were clearly affected under the stress of both pollutants, implying the potential damage to the photosynthetic system of test plants, mainly by decreasing the content of chlorophyll a and b. Results of DnBP and DEHP phytotoxicity to the primary growth of test plants has provided information for the assessment of their environmental risk in the soil and also forms a basis for the further analysis of their toxic effects over the whole growth period of different plant species.

关键词： phytotoxicity di-n-butyl phthalate bis(2- ethylhexyl) phthalate malondialdehyde plant pigments(chlorophyll a b and carotinoids)