Methyl-β-cyclodextrin enhanced biodegradation of polycyclic aromatic hydrocarbons and associated microbial activity in contaminated soil

作     者：Peter Christie 

作者机构：Agri-Environment Branch Agri-Food and Biosciences Institute Newforge Lane Belfast BT9 5PX United Kingdom 

出 版 物：《Journal of Environmental Sciences》 (环境科学学报（英文版）)

年 卷 期：2012年第24卷第5期

页      面：926-933页

核心收录：

学科分类：12[管理学] 1204[管理学-公共管理] 082803[工学-农业生物环境与能源工程] 07[理学] 08[工学] 0828[工学-农业工程] 09[农学] 0903[农学-农业资源与环境] 120405[管理学-土地资源管理] 0713[理学-生态学] 

基　　金：supported by the Natural Science Foundation of Jiangsu Province (No. BK2009016) the Chinese National Environmental Protection Special Funds for Scientific Research on Public Causes (No. 2010467016) the National High Technology Research and Development Program (863) of China (No. 2007AA061101) 

主　　题：polycyclic aromatic hydrocarbons methyl-β-cyclodextrin biodegradation Paracoccus sp. strain HPD-2 microbial activity 

摘      要：The contamination of soils by polycyclic aromatic hydrocarbons （PAHs） is a widespread environmental problem and the remediation of PAHs from these areas has been a major concern. The effectiveness of many in situ bioremediation systems may be constrained by low contaminant bioavailability due to limited aqueous solubility or a large magnitude of sorption. The objective of this research was to evaluate the effect of methyl-β-cyclodextrin （MCD） on bioaugmentation by Paracoccus sp. strain HPD-2 of an aged PAH-contaminated soil. When 10% （W/W） MCD amendment was combined with bioaugmentation by the PAH-degrading bacterium Paracoccus sp. strain HPD-2, the percentage degradation of total PAHs was significantly enhanced up to 34.8%. Higher counts of culturable PAH- degrading bacteria and higher soil dehydrogenase and soil polyphenol oxidase activities were observed in 10% （W/W） MCD-assisted bioaugmentation soil. This MCD-assisted bioaugmentation strategy showed significant increases （p 〈 0.05） in the average well color development （AWCD） obtained by the BIOLOG Eco plate assay, Shannon-Weaver index （H） and Simpson index （λ） compared with the controls, implying that this strategy at least partially restored the microbiological functioning of the PAH-contaminated soil. The results suggest that MCD-aided bioaugmentation by Paracoccus sp. strain HPD-2 may be a promising practical bioremediation strategy for aged PAH-contaminated soils.