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The apoptosis of HEL cells induced by hydroxyurea

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Cell Research 1997年第1期7卷 91-97页

作者： GUI CHANG YUN CHU JIANG HENG YUE XIE RUO LAN QIAN(Shanghai Institute of Cell Biology, Chinese Academy of Sciences, Shanghai 200031) Shanghai Institute of Cell Biology Chinese Academy of Sciences Shanghai 200031&#x0002A Dedicated to Professor Lu Ji SHI's 80th birthday† The project was supported by National Natural Science Foundation of ChinaReceived 19March1997 Revised 9April1997 accepted 5june1997

Hydroxyurea has been used to synchronize cultured cells to S-phase and used to treat patients with sicklecell anemia. Recently, we found that hydroxyurea can induce the apoptosis of HEL (human erythroleukemia) *** induced HEL cells showed ultrastructurally chromatin condensation with regular crescents at the nuclear edges and apoptotic bodies. However, the cells of K562, another human erythroleukemia cell line, did not show such morphological changes. Under fluoroscope, the HEL cells after induction often displayed a clear reduction in nuclear diameter and nuclear chromatin cleavage and condensation and the presence of nuclear ring and apoptotic bodies. Analysis with flow cytometry showed that the percentage of apoptotic cells is about 30-40% after HEL cells were induced by hydroxyurea for 3 days. DNA ladder can be observed by electrophoretic analysis.

关键词： HEL cells hydroxyurea apoptosis

Increment of hFIX expression with endogenous intron 1 in vitro

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Cell Research 1997年第1期7卷 21-29页

作者： ZHENG BING XIAO YUN QIU MIN TAN YONG NA XING DARU LU JING LUN XUE XIN FANG QIU(Institute of Genetics, Fudan Univerisity, Shanghai 200433) The first two authors made equally significant contributions to this work.* This paper is dedicated to Professor Lu Ji SHI on the 80th anniversary of his birthdayReceived 27September1996 Revised 21April1997 accepted 29May1997

This paper probes into the feasibility of increasing expression level of hFIX gene with endogenous nitron 1 sequence. hFIX minigene was obtained with middle sequence truncated nitron 1 inserted into the relative site of hFIX cDNA, and plasmid vector pKG5i'IX, retroviral vector GINaCi'IX were constructed. These vectors were transduced into target cells of PA317, C2C12, primary rabbit skin fibroblasts (RSF) and primary human skin fibroblasts (HSF). The expression level of mixed colonies are PA317/pKG5i'IX, 151 "g/106 cells/24h; PA317/GINaCi'IX, 308ng/106 cells/24 h; C2C12/G1 NaCi'IX, 186 ng/106 cells/24 h; RSF/GINaCi'IX, 1929 ng/106 cells/24 h; HSF/GlNaCi'IX, 1646 ng/106 cells/ 24 h. These results indicated that hFIX minigene with nitron 1 is able to increase the expression level to about 3 times of that of hFIX cDNA. Meanwhile, in order to study the application of hFIX minigene in the retroviral-mediated gene transfer system and refrain from nitron splicing during viral production, a retroviral vector GlNaCi'IXR with reversely inserted hFIX minigene expression cassette was constructed. The expression level of reverse constructor in PA317 cells was 390 ng/106 cells/24 h with 79% of bioactivity. PCR detection of HT/GlNaCi'IXR cells infected with PA317/ClNaCi'IXR supernatant confirmed the existence of nitron 1 sequence. These results suggested that expression vector with forward-inserted intronl-carrying hFIX expression cassette can be used in directed gene Human factor IX expression with nitron transfer, but when using the retroviral-mediated gene transfer system, reversely-inserted intronl-carrying hFIX expression cassette should be considered.

关键词： Intron 1 human clotting factor IX (hFIX) gene transfer gene expression reverse inserted sequence

Association of DNA with nuclear matrix in in vitro assembled nuclei induced by rDNA from Tetrahymena shanghaiensis in Xenopus egg extracts

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Cell Research 1997年第1期7卷 107-117页

作者： CHEN YING BO ZHANG XIU FEN LI ZHONG HE ZHAI(Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing 100871) Department of Cell Biology and Genetics College of Life Sciences Peking University Beijing 100871On the occasion of Professor Lu Ji SHI's (L. C. Sze) eightieth birthday we present this paper and extend our sincere greetings to Professor SHI. As we mentioned in our paper in early 1950's it is Professor SHI who first studied the behavior of exogenous homologous desoxyribose nucleoprotein (chromatin) in amphibian eggs. His work later led to the study of nuclear assembly in cell-free system. No words can be said too much to describe Professor SHI's achievements in this field. Since the beginning of our research in this field in 1989 Professor SHI always shows high interests in our work exchanges ideas with us and encourages us. We want to express our deep indebtedness to Professor SHI for his many kindness.Received 31March1997 Revised 7May1997 accepted 6june1997

The nuclei assembled from exogenous DNA or chromatin in egg extracts resemble their in vivo counterparts in many aspects. However, the distribution pattern of DNA in these nuclei remains unknown. We introduced rDNA from the macronuclei of Tetrabymena into Xenopus cellfree extracts to examine the association of specific DNA sequences with nuclear matrix (NM) in the nuclei assembled in vitro. Our previous works showed the 5'NTS (nontranscription sequences) of the rDNA specifically bind to the NM system in the macronuclei. We show now the rDNA could induce chromatin assembly and nuclear formation in Xenopus cell-free system. When we extracted the NM system and compared the binding affinity of different regions of rDNA with the NM system, we found that the 5'NTS still hold their binding affinity with insoluble structure of the assembled nuclei in the extracts of Xenopus eggs.

关键词： Nuclear assembly nuclear matrix Xenopus egg extracts Tetrahymena rDNA

Chromatin remodeling regulated by steroid and nuclear receptors

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Cell Research 1997年第2期7卷 127-142页

作者： ALAN P WOLFFE(Laboratory of Molecular Embroyology Nat’l Institute ofChild Health and Human Development NIH Bldg, 18T,Rm. 106 Bethesda, MD 20892-5431 USA. e-mail:awlme @helix. nih.gov) Laboratory of Molecular Embroyology Nat'l Institute of Child Health and Human Development NIH Bldg 18T Rm. 106 Bethesda MD 20892-5431 USA. e-mail: awlme@helix.nih.govReceived 27October1997 accepted 4November1997.

Coactivators and corepressors regulate transcriptionby controlling interactions between sequence-specific transcription factors, the basal transcriptional machinery andthe chromatin environment. This review consider the access of nuclear and steroid receptors to chromatin, theiruse of corepressors and coactivators to modify chromatinstructure and the implications for transcriptional *** assembly of specific nucleoprotein architectures andtargeted histone modification emerge as central controlling elements for gene expression.

关键词： Steroid receptor nuclear receptor coactitvator corepressor transcriptional activation nucleosome positioning histone modification transcriptional repression

Garnet-bearing Granulite Facies Rock Xenoliths from Late Mesozoic Volcaniclastic Breccia, Xinyang, Henan Province

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Acta Geologica Sinica(English Edition) 2001年第4期75卷 445-451页

作者： ZHENG Jianping, SUN Min, LU Fengxiang, WANG Chunyangand ZHONG Zengqiu Faculty of Earth Sciences, China University of Geosciences, Wuhan 430074, China Department of Earth Sciences, The University of Hong Kong, China Faculty of Earth Sciences China University of Geosciences Wuhan 430074 China Department of Earth Sciences The University of Hong Kong China [?]3Zheng Jianping Born in 1964 graduated from the China University of Geosciences (Wuhan) with a PhD degree in 1997 mainly engaged in teaching and scientific researches on petrology and chemical geodynamics. E-mail: jpzheng@***.

This paper presents the primary results of petrologic, mineralogical and petrochemical studies of garnet-bearing granulite facies rock xenoliths from Xinyang, Henan Province. These xenoliths, which are found in a pipe of late Mesozoic volcaniclastic breccia, are of high density (3.13–3.30 g/cm3) and high seismic velocity (Vp = 7.04–7.31 km/s), being products of underplating of basaltic magmas and had experienced granulite facies metamorphism. The underplating and metamorphism took place before the eruption of the host rock. Petrographical studies and equilibrium T-P calculations show that these xenoliths were captured at a 49 km depth and experienced at least a 16 km uplift before they were captured. The dynamics of the uplift could be related to the continent-continent collision between the North China plate and the Yangtze plate during the Triassic.

关键词： granulite facies mafic rock xenolith uplift Xinyang

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1997年生产资料市场综合分析

中国流通经济 1997年第1期11卷 5-8页

作者：胡少维 1997年生产资料市场综合分析

１９９７年生产资料市场综合分析胡少维国家信息中心经济预测部１９９６年以来，我国的生产资料市场比较稳定，主要物资产品保持了供求平衡或供略大于求的基本格局。物资企业经营仍然困难，亏损继续扩大，购销稳中偏淡，价格低迷，１－... 详细信息

１９９７年生产资料市场综合分析胡少维国家信息中心经济预测部１９９６年以来，我国的生产资料市场比较稳定，主要物资产品保持了供求平衡或供略大于求的基本格局。物资企业经营仍然困难，亏损继续扩大，购销稳中偏淡，价格低迷，１－１０月份生产资料价格总指数同比下降...

关键词：生产资料市场物资市场物资经济物资流通

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Analysis of Mechanical Properties of Injection Molded Short Glass Fibre (SGF)/Calcite/ABS Composites

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Journal of Materials Science & Technology 1997年第5期13卷 389-396页

作者： Fu, S.Y. Lauke, B. Institute for Polymer Research Dresden e.V Hohe Str.6 01069 Dresden Germany

Acrylonitrile-butadiene-Styrene (ABS), with and without calcium carbonate (calcite) particles,was used as the matrix for reinforcement with as-received short-glass fibres (were originallytreated by the manufacturer) and sized short-glass fibres with two amino-silane coupling *** calcite particle content is 0, 11.7 and 23.5 vol. pct for the matrices. The glass fiber contentis 0, 10 and 15 vol. pct. The matrix materials and corresponding composites were compoundedusing a twin screw extruder and dumbbell-shaped tensile bars were prepared with an injectionmolding process. The tensile and flexural properties as well as the unnotched and notchedCharpy impact energies of short glass fibre/calcite/ABS composites were studied in this *** effects of fibres, fibre surface treatments and particles on these mechanical properties ofthe composites were discussed in detail. An importarit information was obtained, which is thatthe tensile and flexural strengths of hybrid SGF/calcite/ABS composites are the same as thoseof corresponding fibre composites when the ratio of the interfacial adhesion strength betweenparticles and matrix to that between fibres and matrix is higher than certain value. otherwise theformer are lower than the latter.

关键词： ABS Calcite/ABS Composites SGF Analysis of Mechanical Properties of Injection Molded Short Glass Fibre

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理性的呼唤:中国《行政诉讼法》实施现状调查报告--纪念《行政诉讼法》实施十周年

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苏州大学学报（哲学社会科学版） 2001年第Z1期17卷 301-312页

作者：方宁孙静齐建东徐汉松储莹苏州大学法学院1997级学生苏州大学法学院1997级学生苏州大学法学院1997级学生苏州大学法学院1997级学生苏州大学法学院1997级学生

　　一、概述　　纵观人类历史,人类几乎所有的悲与喜、泪水与欢笑都和行政权力有关.我国自夏启建立第一个国家开始,逐渐形成高度集权的行政体制,创造了令世人惊讶的文明.但是,我们的历史却呈现出盛与衰的循环往复的现象,而这一切无不... 详细信息

　　一、概述　　纵观人类历史,人类几乎所有的悲与喜、泪水与欢笑都和行政权力有关.我国自夏启建立第一个国家开始,逐渐形成高度集权的行政体制,创造了令世人惊讶的文明.但是,我们的历史却呈现出盛与衰的循环往复的现象,而这一切无不与我们高度集中的行政权力有关,与我们所奉行的《天下大权,唯一人操之"、"圣躬独裁"等行政观念有关.可以说,行政权力犹如一柄双刃剑,既可造福于亿万人民,也可使天下生灵涂炭,真是有一点"成也萧何,败也萧何'的意味.……

关键词：中国行政诉讼法实施现状行政权力行政体制行政观念历史高度集权双刃剑盛与衰悲与喜循环文明人民国家独裁

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德育在邓小平教育思想中的地位

山东干部函授大学学报(理论学习) 1999年第10期 46-47页

作者：盖敬刚东营分校1997级经管专业学员

邓小平教育思想是以提高整个中华民族素质,多出人才,出好人才,发展经济,增强综合国力为目的的,主张智育与德育并重,通过学科教育渗透思想教育,使学生德、智、体全面协调发展。教育的真谛是育人,育人的核心是人格的教育。人的质量取决... 详细信息

邓小平教育思想是以提高整个中华民族素质,多出人才,出好人才,发展经济,增强综合国力为目的的,主张智育与德育并重,通过学科教育渗透思想教育,使学生德、智、体全面协调发展。教育的真谛是育人,育人的核心是人格的教育。人的质量取决于德育的质量。在邓小平教育思想中,德育工作占有非常重要的地位。所谓德育,是根据一定社会道德规范的要求,对青少年进行的思

关键词：邓小平教育思想智育社会道德规范地位