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Photoexcited CRYPTOCHROME 1 Interacts Directly with G-Protein 13 Subunit AGB1 to Regulate the DNA-Binding Activity of HY5 and Photomorphogenesis in Arabidopsis

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Molecular Plant 2018年第10期11卷 1248-1263页

作者： Hongli Lian Pengbo Xu Shengbo He Jun Wu Jian Pan Wenxiu Wang Feng Xu Sheng Wang Junsong Pan Jirong Huang Hong-Quan Yang School of Agriculture and Biology Shanghai Jiao Tong University Shanghai 200240 China School of Life Sciences and biotechnology Shanghai Jiao Tong University Shanghai 200240 China College of Life and Environmental Sciences Shanghai Normal University Shanghai 200234 China State Key Laboratory of Genetic engineering Collaborative Innovation Center for Genetics and Development School of Life Sciences Fudan University Shanghai200438 China These authors contributed equally to this article.

Light and the heterotrimeric G-protein are known to antagonistically regulate photomorphogenesis in Arabidopsis. However, whether light and G-protein coordinate the regulation of photomorphogenesis is largely unknown. Here we show that the blue light photoreceptor cryptochrome 1 （CRY1） physically inter-acts with the G-protein β subunit, AGB1, in a blue light-dependent manner. We also show that AGB1 directly interacts with HY5, a basic leucine zipper transcriptional factor that acts as a critical positive regulator of photomorphogenesis, to inhibit its DNA-binding activity. Genetic studies suggest that CRY1 acts partially through AGB1, and AGB1 acts partially through HY5 to regulate photomorphogenesis. Moreover, we demonstrate that blue light-triggered interaction of CRY1 with AGB1 promotes the dissociation of HY5 from AGB1. Our results suggest that the CRY1 signaling mechanism involves positive regulation of the DNA-binding activity of HY5 mediated by the CRY1-AGB1 interaction, which inhibits the association of AGB1 with HY5. We propose that the antagonistic regulation of HY5 DNA-binding activity by CRY1 and AGB1 may allow plants to balance light and G-protein signaling and optimize photomorphogenesis.

关键词： Arabidopsis cryptochrome heterotrimeric G-protein β subunit AGB1 HY5 photomorphogenesis

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Epigenetics,a mode for plants to respond to abiotic stresses

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Frontiers in Biology 2011年第6期6卷 477-481页

作者： Weihua QIAO Liumin FAN Insititute of Crop Sciences/National Key Facility for Crop Resources and Genetic Improvement Chinese Academy of Agricultural SciencesBeijing 100081China State Key Laboratory of Protein and Plant Gene Research Genetic Engineering Peking-Yale Joint Center of Plant Molecular Genetics and Agro-biotechnologyCollege of Life SciencesPeking UniversityBeijing 100871China

Epigenetics has been becoming a hot topic in recent *** can be mechanisms that regulate gene expression without changing DNA base *** plants epigenetic regulation has been implicated to be a very important phenomenon and mechanism for the regulation of responses to environmental *** signals induce various epigenetic modifications in the genome,and these epigenetic modifications might likely be inherited to the next generation that behaves with enhanced ability to tolerate *** review highlights recent advances in the study of epigenetics in plant stress responses.

关键词： Epigenetics chromatin genome environmental stress histone modification

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Regulation of jasmonate signaling by reversible acetylation of TOPLESS in Arabidopsis

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Molecular Plant 2022年第8期15卷 1329-1346页

作者： Chunpeng An Lei Deng Huawei Zhai Yanrong You Fangming Wu Qingzhe Zhai Alain Goossens Chuanyou Li State Key Laboratory of Plant Genomics National Center for Plant Gene Research(Beijing)Institute of Genetics and Developmental BiologyInnovation Academy of Seed DesignChinese Academy of SciencesBeijing 100101China CAS center for Excellence in Biotic Interactions University of Chinese Academy of SciencesBeijing 100049China State Key Laboratory of Crop Biology College of Horticulture Science and EngineeringShandong Agricultural UniversityTai'an 271018China Department of Plant biotechnology and Bioinformatics Ghent UniversityTechnologiepark 719052 GhentBelgium VIB center for Plant Systems Biology Technologiepark 719052 GhentBelgium

The plant hormone jasmonate(JA)regulates plant immunity and adaptive growth by orchestrating a genome-wide transcriptional *** regulators of JA-responsive gene expression include the master transcription factor MYc2,which is repressed by the conserved Groucho/Tup1-like corepressor TOPLESS(TPL)in the resting ***,the mechanisms underlying TPL-mediated transcriptional repression of MYc2 activity and hormone-dependent switching between repression and de-repression ***,we report the regulation of TPLactivity and JAsignaling byreversibleacetylation of *** found that the histone acetyltransferase GCN5 could mediate TPL acetylation,which enhances its interaction with the NOVEL-INTERACTOR-OF-JAZ(NINJA)adaptor and promotes its recruitment to MYc2 target promoters,facilitating transcriptional ***,TPL deacetylation by the histonedeacetylase HDA6 weakens TPL-NINJA interaction and inhibitsTPL recruitmentto MYC2 target promoters,facilitating transcriptional *** the resting state,the opposing activities of GCN5 and HDA6 maintain TPL acetylation homeostasis,promoting transcriptional repression activity of *** response to JA elicitation,HDA6 expression is transiently induced,resulted in decreased TPL acetylation and repressor activity,thereby transcriptional activation of MYC2 target ***,the GCN5-TPL-HDA6 module main tains the homeostasis of acetylated TPL,thereby determining the transcriptional state of JA-responsive *** findings uncovered a mechanism by which the TPL corepressor activity in JA signaling is activelytuned inarapidandreversiblemanner.

关键词： jasmonate signaling transcriptional repression TOPLESS corepressor GCN5 HDA6 acetylation

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MS1 is essential for male fertility by regulating the microsporocyte cell plate expansion in soybean

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Science China(Life Sciences) 2021年第9期64卷 1533-1545页

作者： Xiaolong Fang Xiaoyuan Sun Xiangdong Yang Qing Li Chunjing Lin Jie Xu Wenjun Gong Yifan Wang Lu Liu Limei Zhao Baohui Liu Jun Qin Mengchen Zhang Chunbao Zhang Fanjiang Kong Meina Li Innovative center of Molecular genetics and Evolution School of Life SciencesGuangzhou UniversityGuangzhou 510006China Soybean Research Institute National Engineering Research Center for SoybeanJilin Academy of Agricultural SciencesChangchun 130033China State Key Laboratory of Rice Biology China National Rice Research InstituteChinese Academy of Agricultural SciencesHangzhou 311401China Core Facility and Technical Service center for SLSB School of Life Sciences and BiotechnologyShanghai Jiao Tong UniversityShanghai 200240China Cereal&Oil Crop Institute Hebei Academy of Agricultural and Forestry SciencesShijiazhuang 050031China

Male sterility is an essential trait in hybrid seed production,especially for monoclinous and autogamous food *** male-sterile ms1 mutant has been known for more than 50 years and could be instrumental in making hybrid ***,the gene responsible for the male-sterile phenotype has remained ***,we report the map-based cloning and characterization of the MS1 gene in ***1 encodes a kinesin protein and localizes to the nucleus,where it is required for the male meiotic cytokinesis after telophase Ⅱ.We further substantiated that MS1 colocalizes with microtubules and is essential for cell plate formation in soybean male gametogenesis through *** ms1 and CRISPR/Cas9 knockout mutants show complete male sterility but are otherwise phenotypically normal,making them perfect tools for producing hybrid *** identification of MS1 has the practical potential for assembling the sterility system and speeding up hybrid soybean breeding.

关键词： soybean male sterility ms1 kinesin cytokinesis hybrid breeding

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Exploration of sea anemone-inspired high-performance biomaterials with enhanced antioxidant activity

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Bioactive Materials 2022年第4期7卷 504-514页

作者： Lulu Wang Xiaokang Zhang Pingping Xu Jicheng Yan Yuzhong Zhang Hainan Su Chengjun Sun Qiang Lu Weizhi Liu Sars-Fang Centre MOE Key Laboratory of Marine Genetics and BreedingCollege of Marine Life SciencesOcean University of ChinaQingdao266003China Laboratory for Marine Biology and biotechnology Pilot National Laboratory for Marine Science and TechnologyQingdao266071China State Key Laboratory of Microbial Technology Marine Biotechnology Research CenterShandong UniversityQingdao266237China Key Laboratory of Marine Eco-environmental Science and Technology Marine Bioresource and Environment Research CenterFirst Institute of OceanographyMinistry of Natural Resources(MNR)Qingdao266061China National engineering Laboratory for Modern Silk Collaborative Innovation Center of Suzhou Nano Science and TechnologySoochow UniversitySuzhou215123China

Antioxidant biomaterials have attracted much attention in various biomedical fields because of their effective inhibition and elimination of reactive oxygen species(ROS)in pathological ***,the difficulty in ensuring biocompatibility,biodegradability and bioavailability of antioxidant materials has limited their further *** bioavailable antioxidant materials that are derived from natural resources are urgently ***,an integrated multi-omics method was applied to fabricate antioxidant biomaterials.A key cysteine-rich thrombospondin-1 type Ⅰ repeat-like(TSRL)protein was efficiently discovered from among 1262 adhesive components and then used to create a recombinant protein with a yield of 500 mg L^(-1).The biocompatible TSRL protein was able to self-assemble into either a water-resistant coating through Ca^(2+)-mediated coordination or redox-responsive hydrogels with tunable physical *** TSRL-based hydrogels showed stronger 1,1-diphenyl-2-picrylhydrazyl(DPPH)radical scavenging rates than glutathione(GSH)and ascorbic acid(Aa)and protected cells against external oxidative stress significantly more *** topically applied to mice skin,TSRL alleviated epidermal hyperplasia and suppressed the degradation of collagen and elastic fibers caused by ultraviolet radiation B(UVB)irradiation,confirming that it enhanced antioxidant activity in *** is the first study to successfully characterize natural antioxidant biomaterials created from marine invertebrate adhesives,and the findings indicate the excellent prospects of these biomaterials for great applications in tissue regeneration and cosmeceuticals.

关键词： Sessile marine organisms Antioxidant biomaterials Water-resistant coatings Redox-responsive hydrogels Photoaging

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Robust Transcriptional Activation in Plants Using Multiplexed CRISPR-Act2.0 and mTALE-Act Systems

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Molecular Plant 2018年第2期11卷 245-256页

作者： Levi G. Lowder Jianping Zhou Yingxiao Zhang Aimee Malzahn Zhaohui Zhong Tzung-Fu Hsieh Daniel F. Voytas Yong Zhang Yiping Qi Department of Biology East Carolina University Greenville NC 27858 USA Department of biotechnology School of Life Science and Technology Center for informational Biology University of Electronic Science and Technology of ChinaChengdu 610054 China Department of Plant Science and Landscape Architecture University of Maryland College Park MD 20742 USA Department of Plant and Microbial Biology and Plants for Human Health Institute North Carolina State University North Carolina Research Campus KannapolisNC 28O81 USA Department of genetics Ceil Biology and Development Center for Genome Engineering University of Minnesota Minneapolis MN 55455 USA Institute for Bioscience and biotechnology Research University of Maryland Rockville MD 20850 USA

User-friendly tools for robust transcriptional activation of endogenous genes are highly demanded in plants. We previously showed that a dCas9-VP64 system consisting of the deactivated CRISPR- associated protein 9 （dCasg） fused with four tandem repeats of the transcriptional activator VP16 0/1=64） could be used for transcriptional activation of endogenous genes in plants. In this study, we developed a second generation of vector systems for enhanced transcriptional activation in plants. We tested multiple strategies for dCasg-based transcriptional activation, and found that simultaneous recruitment of VP64 by dCas9 and a modified guide RNA scaffold gRNA2.0 （designated CRISPR-Act2.0） yielded stronger transcrip- tional activation than the dCas9-VP64 system. Moreover, we developed a multiplex transcription activator- likeeffector activation （mTALE-Act） system for simultaneous activation of up to four genes in plants. Our results suggest that mTALE-Act is even more effective than CRISPR-Act2.0 in most cases tested. In addition, we explored tissue-specific gene activation using positive feedback loops. Interestingly, our study revealed that certain endogenous genes are more amenable than others to transcriptional activation, and tightly regulated genes may cause target gene silencing when perturbed by activation probes. Hence, these new tools could be used to investigate gene regulatory networks and their control mechanisms. Assembly of multiplex CRISPR-Act2.0 and mTALE-Act systems are both based on streamlined and PCR-independent Golden Gate and Gateway cloning strategies, which will facilitate transcriptional activation applications in both dicots and monocots.

关键词： CRISPR gRNA2.0 MS2-VP64 TALE-VP64 multiplex transcriptional activation

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Assessment of maintenance oral Etoposide following induction chemotherapy with gemcitabine and cisplating in chemonaive extensive small cell lung cancer

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The Chinese-German Journal of Clinical Oncology 2010年第10期9卷 569-573页

作者： Lobna R Ezz Elarab Manal M Abdel Wahab Menha Swellam Zakaria Mostafa Radiation Oncology&Nuclear Medicine Faculty of MedicineAin Shams University Biochemistry Department Genetic Engineering and Biotechnology Research DivisionNational Research Center Abbasia Chest Hospital

Objective： The aim of our study was to evaluate the response and tolerability to treatment when using gemcitabine-cisplatin combination （GC）, followed by maintenance therapy of oral etoposide for non-progressive patients in trial to improve progression free survival and overall survival. Methods： Thirty nine patients with extensive small cell lung cancer （SCLC） and ECOG ≤ 2 received 4 cycles of chemotherapy consisting of gemcitabine 1000 mg/m^2 （day 1 and 8） cisplating 75 mgim2 （day 1） every three weeks. Twenty seven non-progressive patients after 4 cycles of chemotherapy were randomized either to receive oral etoposide 50 mg/m^2 for consecutive 15 days every 3 weeks vs no therapy for three months or progression. Results： Thirty nine eligible patients treated with GC, 27 non progressive patients were subsequently randomized to oral etoposide or observation. Median follow-up was 18 months. The overall response rate to GC was 59% and toxicity to oral etoposide was mild. There was improvement in median progression-free survival （PFS） favoring the maintenance arm of 10.5 months vs 7 months （P 〈 0.05）. Median overall survival （OS） had improved towards the maintenance arm （13 vs 11.5 months）. One year survival （60% vs 24%）, 18 months survival （20% vs 5%） favoring the maintenance. Multivariate analysis revealed that age, performance status, maintenance therapy, and response to treatment were independent prognostic factors for OS. Age, maintenance therapy, and response to treatment were highly significant factors for PFS. Conclusion： Gemcitabine-cisplatin is an effective and tolerable regiment for extensive disease of SCLC. The addition of 3 months of oral etoposide in non progressing patients was associated with a significant improvement of PFS and longer OS.

关键词： gemcitabine cisplatin small cell lung cancer

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A dual-RPA based lateral flow strip for sensitive,on-site detection of CP4-EPSPS and Cry1Ab/Ac genes in genetically modified crops

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Food Science and Human Wellness 2024年第1期13卷 183-190页

作者： Jinbin Wang Yu Wang Xiuwen Hu Yifan Chen Wei Jiang Xiaofeng Liu Juan Liu Lemei Zhu Haijuan Zeng Hua Liu Key Laboratory of Agricultural genetics and Breeding Institute of Biotechnology ResearchShanghai Academy of Agricultural SciencesShanghai 201106China Crops Ecological Environment Security Inspection and Supervision center(Shanghai) Ministry of Agriculture and Rural AffairsShanghai 201106China School of Life Science and engineering Lanzhou University of TechnologyLanzhou 730050China School of Public Health Changsha Medical UniversityChangsha 410219China Academician Workstation Changsha Medical UniversityChangsha 410219China

Traditional transgenic detection methods require high test conditions and struggle to be both sensitive and *** this study,a one-tube dual recombinase polymerase amplification(RPA)reaction system for CP4-EPSPS and Cry1Ab/Ac was proposed and combined with a lateral flow immunochromatographic assay,named“Dual-RPA-LFD”,to visualize the dual detection of genetically modified(GM)*** which,the herbicide tolerance gene CP4-EPSPS and the insect resistance gene Cry1Ab/Ac were selected as targets taking into account the current status of the most widespread application of insect resistance and herbicide tolerance traits and their stacked *** diluted plasmids,transgenic standards,and actual samples were used as templates to conduct sensitivity,specificity,and practicality assays,*** constructed method achieved the visual detection of plasmid at levels as low as 100 copies,demonstrating its high *** addition,good applicability to transgenic samples was observed,with no cross-interference between two test lines and no influence from other *** conclusion,this strategy achieved the expected purpose of simultaneous detection of the two popular targets in GM crops within 20 min at 37°C in a rapid,equipmentfree field manner,providing a new alternative for rapid screening for transgenic assays in the field.

关键词： Genetically modifi ed crops On-site detection Lateral fl ow test strips Dual recombinase polymerase amplification (RPA)

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Wheat powdery mildew resistance gene Pm64 derived from wild emmer (Triticum turgidum ***) is tightly linked in repulsion with stripe rust resistance gene Yr5

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The Crop Journal 2019年第6期7卷 761-770页

作者： Deyun Zhang Keyu Zhu Lingli Dong Yong Liang Genqiao Li Tilin Fang Guanghao Guo Qiuhong Wu Jingzhong Xie Yongxing Chen Ping Lu Miaomiao Li Huaizhi Zhang Zhenzhong Wang Yan Zhang Qixin Sun Zhiyong Liu College of Agronomy and biotechnology China Agricultural UniversityBeijing 100193China State Key Laboratory of Plant Cell and Chromosome engineering Institute of Genetics and Developmental Biologythe Innovative Academy of Seed DesignChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China Department of Plant Soil Sciences Oklahoma State UniversityStillwaterOK 74078USA China Rural Technology Development center Beijing 100045China

Stripe rust and powdery mildew are both devastating diseases for durum and common *** of genes conferring resistance to one or more diseases in a single cultivar is an important breeding approach to provide broader spectra of resistances in wheat improvement. A new powdery mildew resistance gene originating from wild emmer(Triticum turgidum ***) backcrossed into common wheat(T. aestivum) line WE35 was identified. It conferred an intermediate level of resistance to Blumeria graminis f. sp. tritici isolate E09 at the seedling stage and a high level of resistance at the adult plant stage. Genetic analysis showed that the powdery mildew resistance in WE35 was controlled by a dominant gene designated Pm64. Bulked segregant analysis(BSA) and molecular mapping indicated that Pm64 was located in chromosome bin 2 BL4-0.50–0.89. Polymorphic markers were developed from the corresponding genomic regions of Chinese Spring wheat and wild emmer accession Zavitan to delimit Pm64 to a 0.55 cM genetic interval between markers WGGBH1364 and WGGBH612, corresponding to a 15 Mb genomic region on Chinese Spring and Zavitan 2 BL, respectively. The genetic linkage map of Pm64 is critical for fine mapping and cloning. Pm64 was completely linked in repulsion with stripe rust resistance gene Yr5. Analysis of a larger segregating population might identify a recombinant line with both genes as a valuable resource in breeding for resistance to powdery mildew and stripe rust.

关键词： Blumeria graminis Genetic linkage map Yellow rust Triticum aestivum Triticum dicoccoides

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Graphene enhances artemisinin production in the traditional medicinal plant Artemisia annua via dynamic physiological processes and miRNA regulation

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Plant Communications 2024年第3期5卷 31-46页

作者： Junfeng Cao Zhiwen Chen Luyao Wang Ning Yan Jialing Lin Lipan Hou Yongyan Zhao Chaochen Huang Tingting Wen Chenyi Li Saeed ur Rahman Zehui Liu Jun Qiao Jianguo Zhao Jie Wang Yannan Shi Wei Qin Tong Si Yuliang Wang Kexuan Tang Frontiers Science center for Transformative Molecules Joint International Research Laboratory of Metabolic and Developmental SciencesPlant Biotechnology Research CenterFudan-SJTU Nottingham Plant Biotechnology R&D CenterSchool of Agriculture and BiologyShanghai Jiao Tong UniversityShanghai 200240China engineering Research center of Coal-based Ecological Carbon Sequestration Technology of the Ministry of Education Key Laboratory of Graphene Forestry Application of National Forest and Grass AdministrationShanxi Datong UniversityDatong 037009China National Key Laboratory of Plant Molecular genetics Institute of Plant Physiology and Ecology/CAS Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghai 200032China Hainan Institute Zhejiang UniversityYongyou Industry ParkYazhou Bay Sci-Tech CitySanya 572000China College of Agriculture and biotechnology Zhejiang UniversityHangzhou 310058China Shandong Provincial Key Laboratory of Dryland Farming Technology College of AgronomyQingdao Agricultural UniversityQingdao 266109China Institute of Millet Crops Hebei Academy of Agriculture&Forestry Sciences/Hebei Branch of China National Sorghum Improvement CenterShijiazhuang 050035China

We investigated the effects of graphene on the model herb Artemisia annua,which is renowned for produc-ing artemisinin,a widely used pharmacological *** growth and biomass were promoted when *** was cultivated with low concentrations of graphene,an effect which was attributed to a 1.4-fold increase in nitrogen uptake,a 15%–22%increase in chlorophyllfluorescence,and greater abun-dance of carbon cycling–related *** to 10 or 20 mg/L graphene resulted in a�60%increase in H2O2,and graphene could act as a catalyst accelerator,leading to a 9-fold increase in catalase(CAT)ac-tivity in vitro and thereby maintaining reactive oxygen species(ROS)***,graphene exposure led to an 80%increase in the density of glandular secreting trichomes(GSTs),in which artemisinin is biosynthesized and *** contributed to a 5%increase in artemisinin content inmature ***-estingly,expression of miR828 was reduced by both graphene and H2O2 treatments,resulting in induction of its target gene AaMYB17,a positive regulator of GST *** molecular and genetic assays showed that graphene-induced H2O2 inhibits micro-RNA(miRNA)biogenesis through Dicers and regulates the miR828–AaMYB17 module,thus affecting GST *** results suggest that graphene may contribute to yield improvement in *** via dynamic physiological processes together with miRNA regulation,and it may thus represent a new cultivation strategy for increasing yield capacity through nanobiotechnology.

关键词： Artemisia annua artemisinin glandular secreting trichomes GSTs miRNA graphene

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