Wheat powdery mildew resistance gene Pm64 derived from wild emmer (Triticum turgidum ***) is tightly linked in repulsion with stripe rust resistance gene Yr5

作     者：Deyun Zhang Keyu Zhu Lingli Dong Yong Liang Genqiao Li Tilin Fang Guanghao Guo Qiuhong Wu Jingzhong Xie Yongxing Chen Ping Lu Miaomiao Li Huaizhi Zhang Zhenzhong Wang Yan Zhang Qixin Sun Zhiyong Liu 

作者机构：College of Agronomy and BiotechnologyChina Agricultural UniversityBeijing 100193China State Key Laboratory of Plant Cell and Chromosome EngineeringInstitute of Genetics and Developmental Biologythe Innovative Academy of Seed DesignChinese Academy of SciencesBeijing 100101China University of Chinese Academy of SciencesBeijing 100049China Department of Plant Soil SciencesOklahoma State UniversityStillwaterOK 74078USA China Rural Technology Development CenterBeijing 100045China 

出 版 物：《The Crop Journal》 (作物学报（英文版）)

年 卷 期：2019年第7卷第6期

页      面：761-770页

核心收录：

学科分类：0710[理学-生物学] 09[农学] 0901[农学-作物学] 0902[农学-园艺学] 

基　　金：supported by the National Key Research and Development Program of China (2017YFD0101004) the Science and Technology Service Network Initiative of Chinese Academy of Sciences (KFJ-STS-ZDTP-024) 

主　　题：Blumeria graminis Genetic linkage map Yellow rust Triticum aestivum Triticum dicoccoides 

摘      要：Stripe rust and powdery mildew are both devastating diseases for durum and common *** of genes conferring resistance to one or more diseases in a single cultivar is an important breeding approach to provide broader spectra of resistances in wheat improvement. A new powdery mildew resistance gene originating from wild emmer(Triticum turgidum ***) backcrossed into common wheat(T. aestivum) line WE35 was identified. It conferred an intermediate level of resistance to Blumeria graminis f. sp. tritici isolate E09 at the seedling stage and a high level of resistance at the adult plant stage. Genetic analysis showed that the powdery mildew resistance in WE35 was controlled by a dominant gene designated Pm64. Bulked segregant analysis(BSA) and molecular mapping indicated that Pm64 was located in chromosome bin 2 BL4-0.50–0.89. Polymorphic markers were developed from the corresponding genomic regions of Chinese Spring wheat and wild emmer accession Zavitan to delimit Pm64 to a 0.55 cM genetic interval between markers WGGBH1364 and WGGBH612, corresponding to a 15 Mb genomic region on Chinese Spring and Zavitan 2 BL, respectively. The genetic linkage map of Pm64 is critical for fine mapping and cloning. Pm64 was completely linked in repulsion with stripe rust resistance gene Yr5. Analysis of a larger segregating population might identify a recombinant line with both genes as a valuable resource in breeding for resistance to powdery mildew and stripe rust.