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Clinicopathologic analysis of esophageal and cardiac cancers and survey of molecular expression on tissue arrays in Chaoshan littoral of China

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World Journal of Gastroenterology 2004年第15期10卷 2163-2167页

作者： MinSu Xiao-YunLi Dong-PingTian Ming-YaoWu Xian-YingWu Hai-HuaHuang De-RuiLi Zhi-ChaoZheng Xiao-HuXu DepartmentofPathology ShantouUniversityMedicalCollegeShantou515031GuangdongProvinceChina SecondAffiliatedHospitalofShantouUniversityMedicalCollege Shantou515031GuangdongProvinceChina TumorHospitalofShantouUniversityMedicalCollege Shantou515031GuangdongProvinceChina

AIM: To investigate clinical and pathologic data of esophageal carcinoma (EC) and cardiac carcinoma (CC) among residents in Chaoshan region of ***: Clinical and pathologic data of 9 650 patients with EC and 4 173 patients with CC in the Chaoshan population were collected and analyzed. Moreover,Chaoshan esophageal carcinoma tissue arrays were made for high-throughput ***: Male to female ratio was 3:1 in patients with EC and 4.75:1 in CC. The average age of the occurrenceof EC was 54.6 years, and of CC was 58.1 years. For both EC and CC, age at diagnosis was a little younger in Chaoshan region than in most other areas. The most commonly affected site of esophageal carcinoma was the middle third of esophagus (72.0%); the second was the lower third (15.3%). The main gross type of esophageal carcinoma was ulcerative type (41.50%); the medullary type was the second (39.6%). Squamous cell carcinoma accounted for the overwhelming majority of esophageal cancer (96.4%);adenocarcinoma accounted for the overwhelming majority of cardiac carcinoma (94.5%). Chaoshan esophageal carcinoma tissue arrays were easily for high-throughput study, and tissue cores with a diameter of 1.5 mm could better keep more structure for molecular expression ***: Both EC and CC are common in *** average occurrence age of EC and CC is younger in Chaoshan than in most other regions of China. The most commonly affected site of esophageal carcinoma was the middle third of esophagus (72.0%). Squamous cell carcinoma accounted for the overwhelming majority of esophageal cancer; adenocarcinoma accounted for the overwhelming majority of cardiac carcinoma. Tissue arrays technology is applicable for rapid molecular profiling of large numbers of cancers in a single experiment.

关键词：临床病理学食道癌强心剂肿瘤分子表达组织排列潮汕市中国鳞状细胞癌

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人肺上皮细胞表达蛋白酶激活受体

第四军医大学学报 2005年第17期26卷 1564-1566页

作者：王海燕何韶衡付意玲方泽曼汕头大学医学院变态反应学与炎症学研究所广东汕头515031

目的: 蛋白酶激活受体(protease-activated receptors, PARs) 广泛分布在多种组织细胞上,但4种PARs在肺上皮细胞的表达情况尚不清楚. 我们用A549细胞研究PARs在肺上皮细胞的表达. 方法:采用逆转录聚合酶链反应(RT-PCR)、流式细胞仪和免... 详细信息

目的: 蛋白酶激活受体(protease-activated receptors, PARs) 广泛分布在多种组织细胞上,但4种PARs在肺上皮细胞的表达情况尚不清楚. 我们用A549细胞研究PARs在肺上皮细胞的表达. 方法:采用逆转录聚合酶链反应(RT-PCR)、流式细胞仪和免疫荧光细胞染色分析技术分析肺上皮细胞系A549细胞PARs的表达情况. 结果:4种PARs在肺上皮细胞上均有不同程度的表达. 结论:肺上皮细胞同时表达PAR 1～4 4种受体,这为进一步研究PARs在肺上皮细胞的功能奠定了基础.

关键词：蛋白酶激活受体肺上皮细胞 RT—PCR 流式细胞术免疫荧光细胞染色

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EFFECTS OF INTENTIONAL HEMODILUTION ON PULMONARY STASIS AND EDEMA IN PATIENTS AND EXPERIMENTAL ANIMALS

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中华医学杂志(英文版) 1993年第1期106卷 17-21页

作者：陈少如郑鸿翱张少民许鸿举陈晴晖庄世才 Department of Pathophysiology Shantou University Medical College Shantou 515031 Department of Thoracic Surgery Second Affiliated Hospital Henan Medical University Zhengzhou 450052

The therapeutic effects of intentional hemodilution （IHD） on 64 patients of rheumatic mitral valvular disease with chronic pulmonary stasis were estimated. The mean hematocrit was decreased from 0.50±0.06 to 0.41±0.04 by withdrawing blood and replacing it with diluent. The whole blood ratio viscosity was decreased from 11.7±2.4 to 6.4±0.8 （P<0.01）, the pulmonary arterial pressure was reduced from 6.4±0.6 to 4.1±0.5 kPa （0.133 kPa=1mmHg）（P<0.01）, the pulmonary vascular resistance brought down from 536.0±108.0 to 315.0±62.0 dynes. s. cm（P<0.01）, the cardiac output increased from 3.4±0.4 to 5.6±0.5 L/min （P<0.01）, the cardiac index increased from 2.2±0.5 to 3.5±0.4 L/min/m（P<0.01）, and venous oxygen saturation and oxygen content increased from 28.5±3.4%, 5.1±0.5% to 56.0±5.0%, 11.3±1.2%, respectively （P<0.01）. The patients’ symptoms and signs improved significantly. The protective role of IHD in the early stage of lethal pulmonary edema induced by epinephrine in 39 rabbits and 27 rats was also observed. The experimental data show that intentional hemodilution increased the animals’ survival rate （P<0.01）, reduced the pulmonary coefficient, permeability index of Evans blue and protein content in broncho-alveolar lavage fluid （BALF）（all P<0.01）, and alleviated the pathomorphological changes of the lungs of animals in Group IHD were alleviated. The results suggest that HID acts well in both chronic pulmonary stasis and acute pulmonary edema.

关键词： VASCULAR-RESISTANCE BLOOD-VISCOSITY

Clinical significance of serum vascular endothelial growth factor in advanced malignant tumors

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The Chinese-German Journal of Clinical Oncology 2008年第10期7卷 611-614页

作者： Yingcheng Lin De Zeng Hongbiao Wang Wenzhao Lin Wen Lin Chaoqun Hong Department of Medical Oncology The Cancer Hospital of Shantou University Medical College Shantou 515031China

Objective： To elucidate the clinical significance of serum vascular endothelial growth factor （VEGF） level in pa- tients with advanced cancer. Methods： Enzyme linked immunosorbent assay （ELISA） was used to determine the serum VEGF concentration in 40 patients with advanced cancer [non-small cell rung cancer （NSCLC）, esophageal cancer （EC） and nasopharyngeal carcinoma （NPC）] before and after chemotherapy and 10 healthy volunteers as control group. Results： The serum VEGF concentrations in 40 cases of advanced cancer patients were significantly higher than those of 10 healthy control cases [（477.07 ± 374.10 ） pg/mL vs （139.09 ± 133.41 ） pg/mL; P = 0.016]. The serum VEGF concentrations in patients with NSCLC, EC and NPC were （518.53 _± 378.99） pg/mL, （399.21 ± 393.69） pg/mL and （500.68 ± 348.48） pg/mL, respectively. The differences were all statistically significant as compared with healthy control group （P values were 0.011,0.044 and 0.019, respectively）. The serum VEGF concentrations of the patients in response to chemotherapy was significantly lower than those of the same patients before they undergoing chemotherapy [（400.41 ± 332.84） pg/mL vs （777.10 ± 666.01） pg/mL; P = 0.034]. Conclusion： The serum VEGF level might be a novel and promising tumor marker of advanced malignancies and a predictor of disease progression, prognosis and therapeutic efficacy,

关键词： vascular endothelial growth factor (VEGF) advanced malignant tumor serum

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Study on expression of PTEN in esophagus carcinoma and SHEE, SHEEMT, E08712 cell lines

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中国组织化学与细胞化学杂志 2004年第3期13卷 351-352页

作者： XiaHuo XianfengXu Centrallaboratory MedicalColledgeofShantouUniversityShantouGuangdong515031P.R.China

Prognostic and clinicopathological features of E-cadherin,α-catenin,β-catenin,γ-catenin and cyclin D_1 expression in human esophageal squamous cell carcinoma

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World Journal of Gastroenterology 2004年第22期10卷 3235-3239页

作者： Ying-ChengLin Ming-YaoWu De-RuiLi Xian-YingWu Rui-MingZheng DepartmentofMedicalOncology TumorHospitalShantouUniversityMedicalCollegeShantou515031GuangdongProvinceChina DepartmentofPathology ShantouUniversityMedicalCollegeShantou515031GuangdongProvinceChina

AIM: To investigate the expression of E-cadherin, α-catenin,β-catenin, γ-catenin and cyclin D1 in patients with esophageal squamous cell carcinoma (ESCC), and analyze their interrelationship with clinicopathological variables and their effects on prognosis. METHODS: Expression of E-cadherin, α-catenin, β-catenin, γ-catenin and cyclin D1 was determined by EnVision or SABC immunohistochemical technique in patients with ESCC consecutively, their correlation with clinical characteristics was evaluated and analyzed by univariate analysis. RESULTS：The reduced expression rate of E-cadherin, α-catenin, β-catenin and γ-catenin was 88.7%, 69.4%, 35.5% and 53.2%, respectively. Cyclin D1 positive expression ratewas 56.5%. Expression of γ-catenin was inversely correlated with the degree of tumor differentiation and lymph node metastasis (x^2=4.183 and x^2=5.035, respectively, P<0.05), whereas the expression of E-cadherin was correlated only with the degree of differentiation (x^2=5.769, P<0.05). Reduced expression of E-cadherin and γ-catenin was associated with poor differentiation of tumor, reduced expression of γ-catenin was also associated with lymph node metastasis. There obviously existed an inverse correlation between level of E-cadherin and γ-catenin protein and survival. The 3-year survival rates were 100% and 56% in E-cadherin preserved expression group and inreduced expression one and were 78% and 48% in γ-catenin preserved expression group and in reduced expression one, respectively. The differences were both statistically significant. Correlation analysis showed the expression level of α-catenin correlated with that of E-cadherin and β-catenin (P<0.05). CONCLUSION: The reduced expression of E-cadherin and γ-catenin, but not α-catenin, β-catenin and cyclin D1, implies more aggressive malignant behaviors of esophageal carcinoma cells and predicts the poor prognosis of patients.

关键词：临床病理学临床特征 E-钙粘素 γ-链 β-链 α-链 D1表达食管鳞状细胞癌肿瘤

Population dynamics of Dremomys pernyi and Callosciurus erythraeus in protective and non-protective pine forests at different ages

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Frontiers in Biology 2007年第2期2卷 242-246页

作者： MEN Xingyuan GUO Xianguo DONG Wenge QIAN Tijun Medical College Shantou UniversityShantou 515031China Dali Medical College Dali UniversityDali 671000China

Four pine forests(6-10,11-15,16-20,and 31-40 year-old)located in the Cangshan Mountain and Erhai Lake National Reserve and 7 pine forests(1-5,6-10,11-15,1620,21-30,31-40,and more than 50 year-old)located in the non-protective area near the national reserve were *** replications of each forest was set and a total of 33 sites were *** each site,we quantified 6 habitat variables(species richness,abundance,and percentage of grasses and shrubs coverage respectively at the bottom layer of forests)within randomly determined 5 mx5 m *** hundred cages were set in five lines at each site to trap small mammals,whose species and numbers were ***-nance of Dremomys pernyi and Callosciurus erythraeus in small mammal communities,time niche breadth,and time niche overlap between the two small mammals were calcu-lated,***-wise regression was used to analyze the relationship between small mammals and habitat *** results indicated that *** occurred earlier than *** in protective pine *** was captured in 6-10 year-old forest initially,and *** was captured in 16-20 year-old forest *** and *** were captured in the 31-40 and 21-30 year-old forests initially in the non-protective area,*** of *** and *** in the 31-40 year-old protective forests were 3 and 3.75 times of those in the same-aged non-protective forests,*** significantly influenced the populations of the two small *** population of *** was positively correlated with the density of shrubs;the population of *** erythraeus was positively correlated with the coverage of shrubs,and negatively correlated with the coverage of *** pernyi and *** erythraeus were important for pine forests to scatter pine *** activities in the non-protective pine forests decreased the vegetation heterogeneity at the bottom layer of pi

关键词： Dremomys pernyi Callosciurus erythraeus population dynamics

Upregulated expression of Ezrin and invasive phenotype in malignantly transformed esophageal epithelial cells

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World Journal of Gastroenterology 2003年第6期9卷 1182-1186页

作者： Zhong-YingShen Li-YanXu Ming-HuaChen En-MinLi Jin-TaoLi Xian-YingWu YiZeng DepartmentofPathology MedicalCollegeofShantouUniversityShantou515031GuangdongProvinceChina DepartmentofBiochemistry MedicalCollegeofShantouUniversityShantou515031China InstituteofVirology ChineseAcademyofPreventiveMedicineBeijing100052China

AIM: To investigate the correlation between ezrin expression and invasive phenotype formation in malignantly transformed esophageal epithelial cells.METHODS: The experimental cell line employed in the present study was originated form the progressive induction of a human embryonic esophageal epithelial cell line (SHEE)by the E6E7 genes of human papillomavirus (HPV) type *** cells at the 35th passage after induction called SHEEIMM were in a state of immortalized phase and used as the control,while that of the 85th passage denominated as SHEEMT represented the status of cells that were malignantly transformed. The expression changes of ezrin and its mRNA in both cell passages were respectively analyzed by RT-PCR and Western blot. Invasive phenotype was assessed in vivo by inoculating these cells into the severe combined immunodeficient (SCID) mice via subcutaneous and intraperitoneal injection, and in vitro by inoculating them on the surface of the amnion membranes, which then was determined by light microscopy and scanning electron microscopy.RESULTS: Upregulated expression of ezrin protein and its mRNA was observed in SHEEMT compared with that in SHEEIMM cells. The SHEEMT cells inoculated in SCID mice were observed forming tumor masses in both visceral organs and soft tissues in a period of 40 days with a special propensity to invading mesentery and pancreas, but did not exhibit hepatic metastases. Pathologically, these tumor cells harboring larger nucleus, nucleolus and less cytoplasm could infiltrate and destroy adjacent tissues. In the in vitro study,the inoculated SHEEMT cells could grow in cluster on the amniotic epithelial surface and intrude into the amniotic stroma. In contrast, unrestricted growth and invasiveness were not found in SHEEIMM cells in both in vivo and in vitroexperiment.CONCLUSION: The upregulated ezrin expression is one of the important factors that are possibly associated with the invasive phenotype formation in malignantly transformed

关键词： Ezrin 基因表达食管上皮细胞人乳头瘤状病毒食管癌

Effects of hepatitis B virus infection on human sperm chromosomes

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World Journal of Gastroenterology 2003年第4期9卷 736-740页

作者： Jian-MinHuang Tian-HuaHuang Huan-YingQiu Xiao-WuFang Tian-GangZhuang Hong-XiLiu Yong-HuaWang, Li-ZhiDeng Jie-WenQiu DepartmentofCellBiologyandMedicalGenetics ShantouUniversityMedicalCollegeShantou515031GuangdongProvinceChina ShantouUniversityHospital Shantou515063GuangdongProvinceChina DepartmentofInternalMedicine SecondAffiliatedHospitalShantouUniversityMedicalCollegeShantou515041GuangdongProvinceChina

AIM: To evaluate the level of sperm chromosome aberrations in male patients with hepatitis B, and to directly detect whether there are HBV DNA integrations in sperm chromosomes of hepatitis B ***: Sperm chromosomes of 14 tested subjects (5healthy controls, 9 patients with HBV infection, including 1with acute hepatitis B, 2 with chronic active hepatitis B, 4with chronic persistent hepatitis B, 2 chronic HBsAg carriers with no clinical symptoms) were prepared using interspecific in vitro fertilization between zona-free golden hamster ova and human spermatozoa, and the frequencies of aberration spermatozoa were compared between subjects of HBV infection and controls. Fluorescence in situ hybridization (FISH) to sperm chromosome spreads was carried out with biotin-labeled full length HBV DNA probe to detect the specific HBV DNA sequences in the sperm ***: The total frequency of sperm chromosome aberrations in HBV infection group (14.8%, 33/223) was significantly higher than that in the control group (4.3%,5/116). Moreover, the sperm chromosomes in HBV infection patients commonly presented stickiness, clumping, failure to staining, etc, which would affect the analysis of sperm chromosomes. Specific fluorescent signal spots for HBV DNA were seen in sperm chromosomes of one patient with chronic persistent hepatitis. In 9 (9/42) sperm chromosome complements containing fluorescent signal spots, one presented 5 obvious FISH spots, others presented 2 to 4signals. There was significant difference of fluorescence intensity among the signal spots. The distribution of signal sites among chromosomes was ***: HBV infection can bring about mutagenic effects on sperm chromosomes. Integrations of viral DNA into sperm chromosomes which are multisites and nonspecific, can further increase the instability of sperm chromosomes. This study suggested that HBV infection can create extensively hereditary effects by alteration genetic constituent and/o

关键词： Adult Chromosomes, Human DNA, Viral Hepatitis B Antigens Hepatitis B Surface Antigens Hepatitis B, Chronic Humans In Situ Hybridization, Fluorescence Karyotyping Male Reference Values Research Support, Non-U.S. Gov't Semen Spermatozoa