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Neuroinflammation revisited through the microglial lens

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Neural Regeneration Research 2025年第7期20卷 1989-1990页

作者： Renato Socodato João B.Relvas institute of Research and Innovation in Health(i3.)and institute for Molecular and Cell Biology(IBMC) University of PortoPortoPortugal Department of Biomedicine Faculty of Medicine of the University of Porto(FMUP)PortoPortugal

Neuroimmunology is emerging as a pivotal field,shedding light on the intricate dialogues between the central nervous system(CNS)and the immune *** exploration is particularly significant in understanding microglia,the CNS’s innate immune cells,beyond the conventional conflation of“neuroinflammation”and“microglial activation.”This conflation has clouded the true complexity of these processes,potentially stalling scientific progress and the development of new *** challenge the long-standing perspectives that have oversimplified these interactions,advocating for a deeper exploration of the dynamic relationship between neuroinflammation and microglial *** dissecting specific molecular pathways,we aim to illuminate their elaborate roles in neuroinflammatory responses,especially in the context of Alzheimer’s disease(AD).Here,neuroinflammation is not merely a passive observer or a direct antagonist but a complex agent in the disease’s *** article calls for a significant paradigm shift towards an integrative,multi-omics approach to *** such a comprehensive framework is crucial for advancing our understanding of neuroinflammatory conditions and paving the way for targeted therapeutic strategies for brain diseases.

关键词： inflammation simplified activation

Structural and functional analysis of a MADS box containing genomic DNA sequence cloned from rice

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Science China(Life Sciences) 1998年第6期41卷 561-568页

作者：孟征刘国振许智宏唐广王宁陈润生朱立煌 1. institute of Genetics Chinese Academy of Sciences 100101 Beijing China 2. Shanghai institute of Plant Physiology Chinese Academy of Sciences 200032 Shanghai China 3. institute of biophysics Chinese Academy of Sciences 100101 Beijing China

A putative MADS box gene (RgMADS1) was cloned by screening a rice genomic library with a heterogeneous MADS box probe derived from \%Antirrhinum majus squamosa\% gene. Sequencing and Southern analysis showed that RgMADS1 contains a highly conserved MADS box sequence, whereas the rice genome contains numbers of MADS box genes. A chimera gene of RgMADS1 under the control of CaMV 3.S promoter was constructed and used to transform \%Arabidopsis.\% Some transformants exhibited abnormal phenotypes in their flowers, which were reflected in the floral structure, number and setting position. Therefore, RgMADS1 is likely a member of rice MADS box gene family and may be involved in the floral establishment and function control in the rice developmental process.

关键词： rice MADS box genes gene cloning homeotic gene.

In silico analysis of lncRNA-miRNA-mRNA signatures related to Sorafenib effectiveness in liver cancer cells

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World Journal of Gastroenterology 2025年第3期31卷 84-102页

作者： Patricia de la Cruz-Ojeda Ester Parras-Martínez Raquel Rey-Pérez Jordi Muntané Functional Genomics of Solid Tumors Laboratory Centre de Recherche des CordeliersParis 75006France Department of Oncology Surgery Cell Therapy and Organ TransplantationInstitute of Biomedicine of SevilleVirgen del Rocio University HospitalSeville 41013Spain Biomedical Research Center for Hepatic and Digestive Diseases CIBERehdMadrid 28029Spain Department of Medical Physiology and biophysics University of SevilleSeville 41009Spain

BACKGROUND Hepatocellular carcinoma(HCC)is the most common subtype of primary liver cancer with varied incidence and epidemiology *** is still a recommended treatment for a large proportion of patients with advanced *** patterns of treatment responsiveness have been identified in differentiated hepatoblastoma HepG2 cells and metastatic HCC SNU449 *** To define the long non-codingRNA-microRNA-mRNA(lncRNA-miRNA-mRNA)predicted signatures related to selected hallmarks of cancer(apoptosis,autophagy,cell stress,cell dedifferentiation and invasiveness)in RNAseq studies using Sorafenib-treated HepG2 and SNU449 *** available software analyses allowed us to establish the lncRNA-miRNA-mRNA regulatory axes following treatment in HepG2 and SNU449 *** HepG2 and SNU449 cells were treated with Sorafenib(10μmol/L)for 24 *** RNA,including small and long RNA,was extracted with a commercial miRNeasy *** was carried out for the identification of changes in lncRNA-miRNA-mRNA regulatory *** MALAT,THAP9-AS1 and SNGH17 appeared to coordinately regulate miR-3.4b-3. and miR-769-5p that led to upregulation of SMAD7,TIRARP,TFAP4 and FAXDC2 in HepG2 ***12,EPB41 L4A-AS1,LINC01578,SNHG12 and GAS5 interacted with let-7b-3.,miR-195-5p and VEGFA in SNU449 *** axes MALAT1/hsamir-3.4b-3./SMAD7 and MALAT1/hsa-mir-769-5p/TFAP4 were of high relevance for Sorafenib response in HepG2 cells,whereas PVT1/hsa-miR-195-5p/VEGFA was responsible for the differential response of SNU449 cells to Sorafenib *** Critical lncRNAs acting as sponges of miRNA were identified that regulated mRNA expression,whose proteins mainly increased the antitumor effectiveness of the treatment(SMAD7,TIRARP,TFAP4,FAXDC2 and ADRB2).However,the broad regulatory axis leading to increased VEGFA expression may be related to the side effect of Sorafenib in SNU449 cells.

关键词： Cell culture Hepatocellular carcinoma Non-coding RNA RNAseq Sorafenib

Secretome of polarized macrophages:potential for targeting inflammatory dynamics in spinal cord injury

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Neural Regeneration Research 2025年第11期20卷 3231-3232页

作者： Andreia Monteiro Susana Monteiro Nuno A.Silva Life and Health Sciences Research institute(ICVS) School of MedicineUniversity of MinhoBragaPortugal ICVS/3.’s Associate Lab PT Government Associated LabBraga/GuimaraesPortugal

Spinal cord injury(SCI)involves an initial traumatic phase,followed by secondary events such as ischemia,increased blood-spinal cord barrier permeability,ionic disruption,glutamate excitotoxicity,and metabolic alterations.A pe rsistent and exagge rated inflammato ry response within the spinal cord accompanies these events(Lima et al.,2022).The complexity and interplay of these mechanisms exacerbate the initial injury,leading to a degenerative process at the injury *** the initial trauma is unavoidable,the secondary injury begins within minutes and can last for months,creating an optimal window for therapeutic intervention.

关键词： alterations injury companies

A STUDY OF DYNAMIC DEVELOPMENT IN RADIOLESION AND CELL DEATH OF EUKARYOTES AFTER EXPOSURE TO IONIZING RADIATION——THE VISUALIZATION OF THE DYNAMIC REACTION BY MEANS OF CYTOCHEMICAL METHOD WITH LACTATE DEHYDROGENASE (LDH) AS NUCLEAR MARKER AND THE RADIAT

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Science China Chemistry 1985年第6期28卷 584-598页

作者：陈德(山仑),徐国瑞,陈玉玲,彭玲,王辉 institute of biophysics Academia Sinica Beijing institute of biophysics Academia Sinica Beijing institute of biophysics Academia Sinica Beijing institute of biophysics Academia Sinica Beijing institute of biophysics Academia Sinica Beijing

The present work deals with the radiation effect on adult rats suffering from acute, whole-body irradiation with cobalt-60 gamma rays of dosages 100R and 3.0R respectively. The morphological alteration of the lymphocytic nucleus and exhibition of eytochemical reaction of LDH in intra- or extra-nuclear region has been observed at the end of predetermined time intervals after irradiation. A close relationship between the percentage of damage of the lymphocytic nucleus and that of positivity of LDH reaction is seen from their variations with time *** technical color microphotographs which exhibit cytochemical reactions, the existence of enzyme LDH in the nucleus so far obscure has been visually recognized. The events happening in enzymic reaction and the leakage of enzyme across the nuclear envelope reflect the dynamic nature. The ultrastructural alteration of nuclear anomalization confirms the dynamic development of inducing radiolesion. The experimental facts inspire us to attempt an explanatio

关键词： LDH AS NUCLEAR MARKER AND THE RADIAT THE VISUALIZATION OF THE DYNAMIC REACTION BY MEANS OF CYTOCHEMICAL METHOD WITH LACTATE DEHYDROGENASE A STUDY OF DYNAMIC DEVELOPMENT IN RADIOLESION AND CELL DEATH OF EUKARYOTES AFTER EXPOSURE TO IONIZING RADIATION

Creative Industries Agglomeration,Regional Innovation and Productivity Growth in China

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Chinese Geographical Science 2014年第2期24卷 258-268页

作者： HONG Jin YU Wentao GUO Xiumei ZHAO Dingtao School of Management University of Science and Technology of China Curtin University Sustainability Policy institute Curtin University 3. Pakenham St

This paper falls into the broad area of economic geography and economics of creativity,and it presents an alternative approach to explain why total factor productivity(TFP)growth is different across China′s *** establishes an empirical model to estimate the spatial agglomeration effects of creative industries on regional TFP growth,using China′s provincial panel data during the period of 2003.to *** found that the creative industries agglomeration(CIA)has significant and positive impact on regional TFP *** result also implies that the CIA can facilitate regional TFP growth through promoting regional innovation instead of improving regional ***,we argue that policy makers should take some measures to retain and establish more creative zones.

关键词： creative industries spatial externality creativity industries agglomeration(CIA) total factor productivity(TFP) economic growth

Two-dimensional crystallization and preliminary structure analysis of LHC-II from cucumber and spinach

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Science China(Life Sciences) 1998年第3期41卷 265-271页

作者：徐伟张兴娄世庆王可玢黄有国匡廷云 1. institute of biophysics Chinese Academy of Sciences 100101 Beijing China 2. Beijing Laboratory of Electron Microscopy Center for Condensed Matter Physics Chinese Academy of Sciences 100080 Beijing China 3. institute of Botany Chinese Academy of Sciences 100093 Beijing China

Large and well ordered two dimensional (2D) crystals of the light harvesting chlorophyll a/b protein complexes (LHC II) from cucumber and spinach chloroplasts were produced by the so called batch method. The two dimensional structures of these crystals were examined at about 1.5 nm resolution by electron microscopy and image processing. The projection maps showed that there were similar, but not identical, structure features between two different LHC II complexes. A comparison between 2D crystal formations of the two different LHC II complexes was done and some factors affecting 2D crystallization of the membrane proteins were analyzed. The relations of the structures of the LHC II complexes to their polypeptide components and Chl a/b ratio were also discussed.

关键词： light harvesting chlorophyll a/b protein complex two dimensional crystallization electron microscopy image processing structure analysis cucumber spinach.

Structure-based simulations complemented by conventional all-atom simulations to provide new insights into the folding dynamics of human telomeric G-quadruplex

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Chinese Physics B 2021年第7期30卷 646-654页

作者： Yun-Qiang Bian Feng Song Zan-Xia Cao Jia-Feng Yu Ji-Hua Wang Shandong Key Laboratory of biophysics Institute of BiophysicsDezhou UniversityDezhou 253023China

The hybrid atomistic structure-based model has been validated to be effective in investigation of G-quadruplex *** this study,we performed large-scale conventional all-atom simulations to complement the folding mechanism of human telomeric sequence Htel24 revealed by a multi-basin hybrid atomistic structure-based ***,the real time-scale of folding rate,which cannot be obtained from the structure-based simulations,was estimated directly by constructing a Markov state *** results show that Htel24 may fold as fast as on the order of milliseconds when only considering the competition between the hybrid-1 and hybrid-2 G-quadruplex ***,in comparison with the results of structure-based simulations,more metastable states were identified to participate in the formation of hybrid-1 and hybrid-2 *** findings suggest that coupling the hybrid atomistic structure-based model and the conventional all-atom model can provide more insights into the folding dynamics of DNA *** a result,the multiscale computational framework adopted in this study may be useful to study complex processes of biomolecules involving large conformational changes.

关键词： molecular dynamics simulation structure-based model all-atom model DNA G-quadruplex

The use of electro-deoxidation in molten salts to reduce the energy consumption of solar grade silicon and increase the output of PV solar cells

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Progress in Natural Science:Materials International 2015年第6期25卷 583-590页

作者： Paul R.Coxon Michael Coto Eimutis Juzeliunas Derek J.Fray Department of Materials Science & Metallurgy University of Cambridge institute of Chemistry Centre for Physical Sciences & Technology 3. Klaip?da University

Solar photovoltaics,based upon silicon,are the most popular form of solar cell with efficiencies around 20%.These efficiencies can be further increased by employing light trapping schemes to minimise optical losses through scattering and reflection which enhances the amount of light absorbed and number of photo-carriers *** approaches employ antireflection coatings（ARCs） or texturise the surface of the silicon disks,so that the structure consists of an array of needles which can absorb most of the ***,these structures are created by leaching the silicon with hydrofluoric-based acids or by reactive ion etching（RIE） *** paper reviews some of the methods for improving the energy efficiency of silicon production,and describes the use of electro-deoxidation of SiO;layers,on silicon,in molten calcium chloride to form nanoporous black silicon（b-Si） *** coating b-Si surface with TiO;,a common ARC,extremely black surfaces with negligible reflectance of about 0.1%,are produced,which can have applications for low-cost high efficiency solar cells.

关键词： Black silicon Solar cell photovoltaics Silicon molten salt Electrochemistry