Structural and functional analysis of a MADS box containing genomic DNA sequence cloned from rice

作     者：孟征 刘国振 许智宏 唐广 王宁 陈润生 朱立煌 

作者机构：Institute of Genetics Chinese Academy of Sciences Beijing China Shanghai Institute of Plant Physiology Chinese Academy of Sciences Shanghai China Institute of Biophysics Chinese Academy of Sciences Beijing China 

出 版 物：《Science China(Life Sciences)》 (中国科学（生命科学英文版）)

年 卷 期：1998年第41卷第6期

页      面：561-568页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

主　　题：rice MADS box genes gene cloning homeotic gene. 

摘      要：A putative MADS box gene (RgMADS1) was cloned by screening a rice genomic library with a heterogeneous MADS box probe derived from \%Antirrhinum majus squamosa\% gene. Sequencing and Southern analysis showed that RgMADS1 contains a highly conserved MADS box sequence, whereas the rice genome contains numbers of MADS box genes. A chimera gene of RgMADS1 under the control of CaMV 35S promoter was constructed and used to transform \%Arabidopsis.\% Some transformants exhibited abnormal phenotypes in their flowers, which were reflected in the floral structure, number and setting position. Therefore, RgMADS1 is likely a member of rice MADS box gene family and may be involved in the floral establishment and function control in the rice developmental process.